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The map label for this gene is rfbD [H]

Identifier: 78355479

GI number: 78355479

Start: 437955

End: 439103

Strand: Reverse

Name: rfbD [H]

Synonym: Dde_0432

Alternate gene names: 78355479

Gene position: 439103-437955 (Counterclockwise)

Preceding gene: 78355480

Following gene: 78355470

Centisome position: 11.77

GC content: 55.61

Gene sequence:

>1149_bases
ATGAAAAAAGCACTCATCACAGGCATCACCGGACAGGACGGTGCCTATCTTGCCGAATTTCTGCTGAACAAAGGCTACGA
GGTGCACGGTATCAAGCGCCGCGCTTCCTCGTTCAACACCGACCGCATCGACCACCTGTATCAGGACCCGCATGTGGACG
GGCGGAAGTTTATTCTGCACTACGGCGACCTGACCGATTCCACAAACCTGATACGGATCATTCAGGAAGTGCGGCCGCAT
GAAATATACAATCTGGCCGCACAGAGCCATGTAAAGGTCTCATTCGACTCTCCGGAATACACGGCCAACACCGATGCGCT
GGGTACTCTGCGCATTCTCGAAGCCATCCGCATACTGGGACTGGAAAAGCATACCCGTTTCTATCAGGCTTCCACTTCAG
AACTATTCGGGCAGGTTCAGGAAATTCCGCAAAAGGAAACAACGCCGTTCTACCCCCGTTCTCCCTACGCCTGCGCCAAG
CTGTACGCATACTGGATTACCGTTAACTACCGTGAAGCATACGGCATTTACGGATGCAACGGCATTCTGTTCAACCATGA
ATCGCCCGTACGCGGCGAAACGTTTGTCACCCGCAAGATAACCCGTGCTCTGGCCCGCATAGCGCTGGGACTGCAGCAAA
AGGTCTATCTGGGCAACATGAACGCCAAACGCGACTGGGGCCATGCCCGCGACTATGTGGAAATGCAGTGGCTCATGCTG
CAGCAGGAACACCCCGAGGACTTTGTCATCGCCACGGGCAGACAGCATTCCGTTCGTGATTTTGTCAATATTTCGGCAGC
TGAAGTCGGTATTTTTCTGGACTGGCAGGGCAGCGGTGCCGAAGAAAAAGGTGTGGTACGGGCCGTGGACGAAACCCGGC
TTGCCGCATGCCTGAACGGTAAAAGCGGCACGGCACGCATGCCGTCGCCCGGTGATGTCATAGTTGAAGTAGACCGGCGT
TATTTCCGCCCGACGGAAGTGGAAACACTGCTGGGCGACCCCTCGCGGGCCAAGGAAAAACTCGGCTGGACGCCGCGCAT
CAGCTTTGAAGAGATGGTCCGTGAAATGGTGCAGACAGATGTGAACATCGCCGCGCGCGACCGCGTTTGCCTGGACAGCG
GCTTCACCGTTTTTGAATACAACGACTAG

Upstream 100 bases:

>100_bases
GCTGCGGCATTCTTTATGCTATTGTCACGGTGGCAACTACGTTGTATGTCCAGAACCTGACAATACCGCATAAAAAGCAA
CACAAACTTTCCGGAACATT

Downstream 100 bases:

>100_bases
ACGCGCCGCAACGTACGCCCGGCGCACGCTGCAGACACATCCGCAATTTTTTTCTGTTTTTCCGGCACGGCCGCATGCAA
CGACGGGCAGGATGCACCGC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKKALITGITGQDGAYLAEFLLNKGYEVHGIKRRASSFNTDRIDHLYQDPHVDGRKFILHYGDLTDSTNLIRIIQEVRPH
EIYNLAAQSHVKVSFDSPEYTANTDALGTLRILEAIRILGLEKHTRFYQASTSELFGQVQEIPQKETTPFYPRSPYACAK
LYAYWITVNYREAYGIYGCNGILFNHESPVRGETFVTRKITRALARIALGLQQKVYLGNMNAKRDWGHARDYVEMQWLML
QQEHPEDFVIATGRQHSVRDFVNISAAEVGIFLDWQGSGAEEKGVVRAVDETRLAACLNGKSGTARMPSPGDVIVEVDRR
YFRPTEVETLLGDPSRAKEKLGWTPRISFEEMVREMVQTDVNIAARDRVCLDSGFTVFEYND

Sequences:

>Translated_382_residues
MKKALITGITGQDGAYLAEFLLNKGYEVHGIKRRASSFNTDRIDHLYQDPHVDGRKFILHYGDLTDSTNLIRIIQEVRPH
EIYNLAAQSHVKVSFDSPEYTANTDALGTLRILEAIRILGLEKHTRFYQASTSELFGQVQEIPQKETTPFYPRSPYACAK
LYAYWITVNYREAYGIYGCNGILFNHESPVRGETFVTRKITRALARIALGLQQKVYLGNMNAKRDWGHARDYVEMQWLML
QQEHPEDFVIATGRQHSVRDFVNISAAEVGIFLDWQGSGAEEKGVVRAVDETRLAACLNGKSGTARMPSPGDVIVEVDRR
YFRPTEVETLLGDPSRAKEKLGWTPRISFEEMVREMVQTDVNIAARDRVCLDSGFTVFEYND
>Mature_382_residues
MKKALITGITGQDGAYLAEFLLNKGYEVHGIKRRASSFNTDRIDHLYQDPHVDGRKFILHYGDLTDSTNLIRIIQEVRPH
EIYNLAAQSHVKVSFDSPEYTANTDALGTLRILEAIRILGLEKHTRFYQASTSELFGQVQEIPQKETTPFYPRSPYACAK
LYAYWITVNYREAYGIYGCNGILFNHESPVRGETFVTRKITRALARIALGLQQKVYLGNMNAKRDWGHARDYVEMQWLML
QQEHPEDFVIATGRQHSVRDFVNISAAEVGIFLDWQGSGAEEKGVVRAVDETRLAACLNGKSGTARMPSPGDVIVEVDRR
YFRPTEVETLLGDPSRAKEKLGWTPRISFEEMVREMVQTDVNIAARDRVCLDSGFTVFEYND

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=362, Percent_Identity=61.0497237569061, Blast_Score=455, Evalue=1e-128,
Organism=Escherichia coli, GI1788366, Length=376, Percent_Identity=68.8829787234042, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI48994969, Length=367, Percent_Identity=26.9754768392371, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788353, Length=261, Percent_Identity=24.5210727969349, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=362, Percent_Identity=59.9447513812155, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17507723, Length=368, Percent_Identity=59.5108695652174, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI133901788, Length=362, Percent_Identity=59.9447513812155, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17539422, Length=362, Percent_Identity=59.9447513812155, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI133901790, Length=362, Percent_Identity=59.9447513812155, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI133901786, Length=362, Percent_Identity=59.9447513812155, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24158427, Length=370, Percent_Identity=58.1081081081081, Blast_Score=450, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 43483; Mature: 43483

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALITGITGQDGAYLAEFLLNKGYEVHGIKRRASSFNTDRIDHLYQDPHVDGRKFILH
CCCCEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEE
YGDLTDSTNLIRIIQEVRPHEIYNLAAQSHVKVSFDSPEYTANTDALGTLRILEAIRILG
ECCCCCCHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
LEKHTRFYQASTSELFGQVQEIPQKETTPFYPRSPYACAKLYAYWITVNYREAYGIYGCN
CHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHHCCEEECC
GILFNHESPVRGETFVTRKITRALARIALGLQQKVYLGNMNAKRDWGHARDYVEMQWLML
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHH
QQEHPEDFVIATGRQHSVRDFVNISAAEVGIFLDWQGSGAEEKGVVRAVDETRLAACLNG
HCCCCCCEEEECCCCCCHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEHHHHHHHHHCCC
KSGTARMPSPGDVIVEVDRRYFRPTEVETLLGDPSRAKEKLGWTPRISFEEMVREMVQTD
CCCCCCCCCCCCEEEEECHHHCCCCHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHCC
VNIAARDRVCLDSGFTVFEYND
CCEEECCCEEECCCCEEEEECC
>Mature Secondary Structure
MKKALITGITGQDGAYLAEFLLNKGYEVHGIKRRASSFNTDRIDHLYQDPHVDGRKFILH
CCCCEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEE
YGDLTDSTNLIRIIQEVRPHEIYNLAAQSHVKVSFDSPEYTANTDALGTLRILEAIRILG
ECCCCCCHHHHHHHHHHCCHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
LEKHTRFYQASTSELFGQVQEIPQKETTPFYPRSPYACAKLYAYWITVNYREAYGIYGCN
CHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHHCCEEECC
GILFNHESPVRGETFVTRKITRALARIALGLQQKVYLGNMNAKRDWGHARDYVEMQWLML
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHH
QQEHPEDFVIATGRQHSVRDFVNISAAEVGIFLDWQGSGAEEKGVVRAVDETRLAACLNG
HCCCCCCEEEECCCCCCHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEHHHHHHHHHCCC
KSGTARMPSPGDVIVEVDRRYFRPTEVETLLGDPSRAKEKLGWTPRISFEEMVREMVQTD
CCCCCCCCCCCCEEEEECHHHCCCCHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHCC
VNIAARDRVCLDSGFTVFEYND
CCEEECCCEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9098074; 10521656 [H]