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The map label for this gene is tyrS

Identifier: 78355275

GI number: 78355275

Start: 213803

End: 214990

Strand: Reverse

Name: tyrS

Synonym: Dde_0228

Alternate gene names: 78355275

Gene position: 214990-213803 (Counterclockwise)

Preceding gene: 78355276

Following gene: 78355274

Centisome position: 5.76

GC content: 57.15

Gene sequence:

>1188_bases
ATGGATCAACAACTCAAGACGATTCTGCGCGGCAGTGCGGAGTTCATCGATGAAGAAGAACTGAAAAAGAAGCTTGCCAA
AGGCAGACCGCTGCGCGTCAAGGCCGGATTCGACCCCACCGCGCCCGACCTGCATCTGGGACACACCGTCCTTATTCACA
AGCTGCGTCATTTTCAGGAGCTGGGCCACACGGTCATCTTTCTAATCGGCGACTTCACCGGCATGATCGGCGACCCTTCG
GGCCGTTCCGAAACCCGCCCGCCGCTCACCCGCGAACAGGTTCTGCAGAACGCCGAGACCTATAAAAAACAGGTCTTCAA
AATTCTGGATCCTGAAAAAACCGAGGTGCGGTTCAACTCCGAATGGATGGACAAATTCGGCGCAGCCGATTTCATCCGTC
TTGCTTCGCGATATACCGTTGCCCGCATGCTGGAACGCGACGACTTTGAAAAGCGCTATCGCAGCAACATGACCATTTCC
ATCCATGAATTTCTGTATCCCCTCGTGCAGGGCTATGACTCCGTGGCGCTTGAAGCCGATGTGGAACTGGGCGGCACCGA
CCAGAAATTCAACCTGCTGGTGGGCAGACATCTGCAGAGCCAGGAAGGTCAGGAACCGCAGTGCGTGCTCACTGTGCCCA
TCCTCGAAGGGCTTGACGGCGTCAAAAAGATGTCAAAGTCGCTGGGCAACTATGTGGGCATAGATGAACCGCCCGCCGAC
ATGTTCGGCAAACTCATGTCCGTTTCCGACGAACTGATGTGGCGCTATTACGAACTTATCACCACCAAAACGCTGGACGA
AATTGCCGAGCTGCAGCGCAGGGTGGCAAGCGGCGAGCTGCATCCCAAAACAGCCAAGGAAGAGCTGGCCGAACACATCA
CCGCGCAGTACCACGGCAGCGATAAAGCCGCCGAAGCGCGTCAGGGCTTCAATGCCGTGTTTGCGCAGGGCTGTGTGCCC
GACGACATGCCCGAGTTCTCGTGTTCCTGCGGCGAAGACAGTACTCCGCCGGCGTTTCTGGCCGATTCCGGGCTTGCCGG
TTCACGCGGCGAGGCAAAACGCCTCATCAAACAGGGAGCCCTTTCGCTGGACGGCGAAAAACTGGACGACCCCAACACGC
CCCTGACTGCCGGCGAGTATGTCGTGCGGCTGGGTAAAAAACGTTTCCTCAGACTTATTGTGCGCTGA

Upstream 100 bases:

>100_bases
TGCGGGGCAGGTGCGCGGCAGACGGCGTCTTTGACATCAGTGCCGTTCTCTGCAACACCAGCCCGATCAAATACAACTAC
ATGGAAGTGTGACAATGGAC

Downstream 100 bases:

>100_bases
GCAGCCTTTACCGGCAGCATATGACACAAAAAACGGAGCATCCGCCCATGGCGGATGCTCTTTTTTTATGCACCGATGCA
ACTGACACCCCCTGACCGGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MDQQLKTILRGSAEFIDEEELKKKLAKGRPLRVKAGFDPTAPDLHLGHTVLIHKLRHFQELGHTVIFLIGDFTGMIGDPS
GRSETRPPLTREQVLQNAETYKKQVFKILDPEKTEVRFNSEWMDKFGAADFIRLASRYTVARMLERDDFEKRYRSNMTIS
IHEFLYPLVQGYDSVALEADVELGGTDQKFNLLVGRHLQSQEGQEPQCVLTVPILEGLDGVKKMSKSLGNYVGIDEPPAD
MFGKLMSVSDELMWRYYELITTKTLDEIAELQRRVASGELHPKTAKEELAEHITAQYHGSDKAAEARQGFNAVFAQGCVP
DDMPEFSCSCGEDSTPPAFLADSGLAGSRGEAKRLIKQGALSLDGEKLDDPNTPLTAGEYVVRLGKKRFLRLIVR

Sequences:

>Translated_395_residues
MDQQLKTILRGSAEFIDEEELKKKLAKGRPLRVKAGFDPTAPDLHLGHTVLIHKLRHFQELGHTVIFLIGDFTGMIGDPS
GRSETRPPLTREQVLQNAETYKKQVFKILDPEKTEVRFNSEWMDKFGAADFIRLASRYTVARMLERDDFEKRYRSNMTIS
IHEFLYPLVQGYDSVALEADVELGGTDQKFNLLVGRHLQSQEGQEPQCVLTVPILEGLDGVKKMSKSLGNYVGIDEPPAD
MFGKLMSVSDELMWRYYELITTKTLDEIAELQRRVASGELHPKTAKEELAEHITAQYHGSDKAAEARQGFNAVFAQGCVP
DDMPEFSCSCGEDSTPPAFLADSGLAGSRGEAKRLIKQGALSLDGEKLDDPNTPLTAGEYVVRLGKKRFLRLIVR
>Mature_395_residues
MDQQLKTILRGSAEFIDEEELKKKLAKGRPLRVKAGFDPTAPDLHLGHTVLIHKLRHFQELGHTVIFLIGDFTGMIGDPS
GRSETRPPLTREQVLQNAETYKKQVFKILDPEKTEVRFNSEWMDKFGAADFIRLASRYTVARMLERDDFEKRYRSNMTIS
IHEFLYPLVQGYDSVALEADVELGGTDQKFNLLVGRHLQSQEGQEPQCVLTVPILEGLDGVKKMSKSLGNYVGIDEPPAD
MFGKLMSVSDELMWRYYELITTKTLDEIAELQRRVASGELHPKTAKEELAEHITAQYHGSDKAAEARQGFNAVFAQGCVP
DDMPEFSCSCGEDSTPPAFLADSGLAGSRGEAKRLIKQGALSLDGEKLDDPNTPLTAGEYVVRLGKKRFLRLIVR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=423, Percent_Identity=26.4775413711584, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1787925, Length=383, Percent_Identity=31.0704960835509, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25145802, Length=372, Percent_Identity=28.2258064516129, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI25145805, Length=307, Percent_Identity=24.7557003257329, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6325160, Length=329, Percent_Identity=25.8358662613982, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI20130383, Length=403, Percent_Identity=26.0545905707196, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_DESDG (Q316W7)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386724.1
- ProteinModelPortal:   Q316W7
- SMR:   Q316W7
- STRING:   Q316W7
- GeneID:   3755088
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0228
- NMPDR:   fig|207559.3.peg.1018
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   DDES207559:DDE_0228-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 44304; Mature: 44304

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQLKTILRGSAEFIDEEELKKKLAKGRPLRVKAGFDPTAPDLHLGHTVLIHKLRHFQE
CCHHHHHHHCCCHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LGHTVIFLIGDFTGMIGDPSGRSETRPPLTREQVLQNAETYKKQVFKILDPEKTEVRFNS
HCCEEEEEEECHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEECH
EWMDKFGAADFIRLASRYTVARMLERDDFEKRYRSNMTISIHEFLYPLVQGYDSVALEAD
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCEEEEEC
VELGGTDQKFNLLVGRHLQSQEGQEPQCVLTVPILEGLDGVKKMSKSLGNYVGIDEPPAD
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHCCHHHHHHHHHHCCCCCCCCCCHH
MFGKLMSVSDELMWRYYELITTKTLDEIAELQRRVASGELHPKTAKEELAEHITAQYHGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
DKAAEARQGFNAVFAQGCVPDDMPEFSCSCGEDSTPPAFLADSGLAGSRGEAKRLIKQGA
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCC
LSLDGEKLDDPNTPLTAGEYVVRLGKKRFLRLIVR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDQQLKTILRGSAEFIDEEELKKKLAKGRPLRVKAGFDPTAPDLHLGHTVLIHKLRHFQE
CCHHHHHHHCCCHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LGHTVIFLIGDFTGMIGDPSGRSETRPPLTREQVLQNAETYKKQVFKILDPEKTEVRFNS
HCCEEEEEEECHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEECH
EWMDKFGAADFIRLASRYTVARMLERDDFEKRYRSNMTISIHEFLYPLVQGYDSVALEAD
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCEEEEEC
VELGGTDQKFNLLVGRHLQSQEGQEPQCVLTVPILEGLDGVKKMSKSLGNYVGIDEPPAD
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHCCHHHHHHHHHHCCCCCCCCCCHH
MFGKLMSVSDELMWRYYELITTKTLDEIAELQRRVASGELHPKTAKEELAEHITAQYHGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
DKAAEARQGFNAVFAQGCVPDDMPEFSCSCGEDSTPPAFLADSGLAGSRGEAKRLIKQGA
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCC
LSLDGEKLDDPNTPLTAGEYVVRLGKKRFLRLIVR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA