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The map label for this gene is dnaN [H]

Identifier: 78355049

GI number: 78355049

Start: 1642

End: 2796

Strand: Direct

Name: dnaN [H]

Synonym: Dde_0002

Alternate gene names: 78355049

Gene position: 1642-2796 (Clockwise)

Preceding gene: 78355048

Following gene: 78355050

Centisome position: 0.04

GC content: 51.6

Gene sequence:

>1155_bases
ATGTATTTAAAAGTTTTCAAAGAGGACGTGATCGACGGACTTCAGAAAGCAGCCAACATCATTCCCGCCAAAACAGGAGC
GGCTTATCTGCGTTCCATCTGGCTGAAAGCTGAAGCGGGTATGGTGCATGTTCTTTCCACCGACTCCAATATCGAGTTCA
GAGGATCGTATAAGGCGGAAGTGACCGAGGAAGGTCTTGCCGGAGTTCAGGGGCGTGCCTTTGTGGATCTTATCCGCAAG
CTTCCTGCCGGAGAGATCACCCTGCATCTTGACCCCAAGACCGGTACGCTGGGCATTGAACAGGGACGCCGCAAATATAC
GCTGCCCACCAATGACACCGCATGGTTCCAGAATTTTTCTGATTATCCGGAAAGCGGCAGTGTTTTCTGGTCCGGTGATT
TTCTGCAGGAACTCATCGACAAGCTGTCTTTCTGCATAAGCGACGAAGACACCATGGAAGCCATTGCCTGCATGAGCATG
AAACCCCGTCAGGACAACGCCATTGAAGCCTGCGGACTGAACGGCCACCAGTTTGCCATGCAGCGGTTCCTCAATGACGA
TATTCATGCGCTGCTGCCTGCCGAAGGCATTCTGGTGCAGAAAAAATATCTTATGGAGCTGAAGAAATGGCTCGGTGCCG
ATGAGATAGAACTGAACATTGACAACAAGCGTCTTTTCTTCCGCACGGGTGATAAGCGTGAAACATTCAGTTTGCCTCTT
TCGTATTACCAGTATCCGGACTATACCAGCTTTGTTTCCAAACTGAGCACACCCGATGCCGGAGCGCTGGAAGTAAACCG
CAAGGATATGATGGCCGCTCTGGACCGCGTGCTTATTTTCAATACCGACAACAACCGCTGCACCTACTTTGATCTTGAAG
CAGGTGAAGTTTCTCTGTCCTCTCAGGGACAGGAGGTCGGTTCCGCGTCCGAATCGCTGGAAGGCACCTACGACGGCAGC
CTTAAGCGCATAGCCTTTCCCACGCGTAATCTTATCGAGATAATGAACCACTATCAGTCGGACTCCATGCGCTTCACGCT
GACCGGTACCGAAGGCCCCTGCGGTCTGACCGGTGAAAACGACCCCGACTATCTGGTAATCGTCATGCCCATGAAGATAG
TGGAAGAGACGTACTACAGCGAGGAAGAAGTCTAA

Upstream 100 bases:

>100_bases
CACAATACAGGCACTTAAAATAATCATATATAAAAAGTAGTTATGCAGGTTGCCTACATAGGAACCGCCCCTACTACATA
ATACAAAGGGAGAATACCTT

Downstream 100 bases:

>100_bases
ATGACCAACGAGACACAAAAGCAGAGCTACGACGCGGCGAACATCACCGTTCTTGAGGGGTTGTCCGCTGTGCGCATGCG
CCCTGCCATGTACATAGGCA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MYLKVFKEDVIDGLQKAANIIPAKTGAAYLRSIWLKAEAGMVHVLSTDSNIEFRGSYKAEVTEEGLAGVQGRAFVDLIRK
LPAGEITLHLDPKTGTLGIEQGRRKYTLPTNDTAWFQNFSDYPESGSVFWSGDFLQELIDKLSFCISDEDTMEAIACMSM
KPRQDNAIEACGLNGHQFAMQRFLNDDIHALLPAEGILVQKKYLMELKKWLGADEIELNIDNKRLFFRTGDKRETFSLPL
SYYQYPDYTSFVSKLSTPDAGALEVNRKDMMAALDRVLIFNTDNNRCTYFDLEAGEVSLSSQGQEVGSASESLEGTYDGS
LKRIAFPTRNLIEIMNHYQSDSMRFTLTGTEGPCGLTGENDPDYLVIVMPMKIVEETYYSEEEV

Sequences:

>Translated_384_residues
MYLKVFKEDVIDGLQKAANIIPAKTGAAYLRSIWLKAEAGMVHVLSTDSNIEFRGSYKAEVTEEGLAGVQGRAFVDLIRK
LPAGEITLHLDPKTGTLGIEQGRRKYTLPTNDTAWFQNFSDYPESGSVFWSGDFLQELIDKLSFCISDEDTMEAIACMSM
KPRQDNAIEACGLNGHQFAMQRFLNDDIHALLPAEGILVQKKYLMELKKWLGADEIELNIDNKRLFFRTGDKRETFSLPL
SYYQYPDYTSFVSKLSTPDAGALEVNRKDMMAALDRVLIFNTDNNRCTYFDLEAGEVSLSSQGQEVGSASESLEGTYDGS
LKRIAFPTRNLIEIMNHYQSDSMRFTLTGTEGPCGLTGENDPDYLVIVMPMKIVEETYYSEEEV
>Mature_384_residues
MYLKVFKEDVIDGLQKAANIIPAKTGAAYLRSIWLKAEAGMVHVLSTDSNIEFRGSYKAEVTEEGLAGVQGRAFVDLIRK
LPAGEITLHLDPKTGTLGIEQGRRKYTLPTNDTAWFQNFSDYPESGSVFWSGDFLQELIDKLSFCISDEDTMEAIACMSM
KPRQDNAIEACGLNGHQFAMQRFLNDDIHALLPAEGILVQKKYLMELKKWLGADEIELNIDNKRLFFRTGDKRETFSLPL
SYYQYPDYTSFVSKLSTPDAGALEVNRKDMMAALDRVLIFNTDNNRCTYFDLEAGEVSLSSQGQEVGSASESLEGTYDGS
LKRIAFPTRNLIEIMNHYQSDSMRFTLTGTEGPCGLTGENDPDYLVIVMPMKIVEETYYSEEEV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=377, Percent_Identity=22.0159151193634, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 43160; Mature: 43160

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLKVFKEDVIDGLQKAANIIPAKTGAAYLRSIWLKAEAGMVHVLSTDSNIEFRGSYKAE
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCEEEEEECCCCEEEECCCCCC
VTEEGLAGVQGRAFVDLIRKLPAGEITLHLDPKTGTLGIEQGRRKYTLPTNDTAWFQNFS
CCHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCEEEECCCCCHHHHCCCC
DYPESGSVFWSGDFLQELIDKLSFCISDEDTMEAIACMSMKPRQDNAIEACGLNGHQFAM
CCCCCCCEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHH
QRFLNDDIHALLPAEGILVQKKYLMELKKWLGADEIELNIDNKRLFFRTGDKRETFSLPL
HHHHCCCHHHHCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCEEECCH
SYYQYPDYTSFVSKLSTPDAGALEVNRKDMMAALDRVLIFNTDNNRCTYFDLEAGEVSLS
HHCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHCEEEEEECCCCEEEEEECCCCCEECC
SQGQEVGSASESLEGTYDGSLKRIAFPTRNLIEIMNHYQSDSMRFTLTGTEGPCGLTGEN
CCCCHHCCHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
DPDYLVIVMPMKIVEETYYSEEEV
CCCEEEEEECHHHHHHHHCCCCCC
>Mature Secondary Structure
MYLKVFKEDVIDGLQKAANIIPAKTGAAYLRSIWLKAEAGMVHVLSTDSNIEFRGSYKAE
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCEEEEEECCCCEEEECCCCCC
VTEEGLAGVQGRAFVDLIRKLPAGEITLHLDPKTGTLGIEQGRRKYTLPTNDTAWFQNFS
CCHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCEEEECCCCCHHHHCCCC
DYPESGSVFWSGDFLQELIDKLSFCISDEDTMEAIACMSMKPRQDNAIEACGLNGHQFAM
CCCCCCCEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEECCCCHHHHHH
QRFLNDDIHALLPAEGILVQKKYLMELKKWLGADEIELNIDNKRLFFRTGDKRETFSLPL
HHHHCCCHHHHCCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCEEECCH
SYYQYPDYTSFVSKLSTPDAGALEVNRKDMMAALDRVLIFNTDNNRCTYFDLEAGEVSLS
HHCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHCEEEEEECCCCEEEEEECCCCCEECC
SQGQEVGSASESLEGTYDGSLKRIAFPTRNLIEIMNHYQSDSMRFTLTGTEGPCGLTGEN
CCCCHHCCHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
DPDYLVIVMPMKIVEETYYSEEEV
CCCEEEEEECHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]