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The map label for this gene is nadA

Identifier: 78224727

GI number: 78224727

Start: 3976623

End: 3977537

Strand: Direct

Name: nadA

Synonym: Gmet_3544

Alternate gene names: 78224727

Gene position: 3976623-3977537 (Clockwise)

Preceding gene: 78224725

Following gene: 78224728

Centisome position: 99.48

GC content: 64.7

Gene sequence:

>915_bases
ATGCAGGCCGATACCATCAAGCAGGAAATCAGGCGGCTCCTCAAGGAGCGCAACGCCGTCCTCCTGGCTCACAACTACAT
GCGGGACGAGGTGCAGGAAATCGCCGACATCACCGGCGACTCCCTGGGGCTCTCCCAAGAGGCGGCCAAAACCGCTGCGG
ACGTGATCGTCTTCTGCGGTGTTCACTTCATGGCCGAGTCGGCATCCATCCTCTCGCCCCATAAGACGGTGCTCCTGCCG
CGGCGCGACGCCGGCTGCCCCATGGCCGACATGGTGACGGTGGAGGGACTCCTGGAGCTGAAGGCCAGGCATCCGGGCGT
GCCGGTTGTCACCTACGTGAACTCCTCGGCGGCAGTGAAGGCCGTGAGCGACATCTGCTGCACCTCCGCCAACGCCGTGA
AGGTGGTCAACTCCCTCCCGGACCGGGAGGTGATCTTCGTCCCCGACCGGAACCTGGGCCAGTTCGTGGCGAAGCAGTCG
GACAAGGCCTTCCACTTCTGGGACGGCTACTGCCCCACCCACGAGCGGCTCAAGGCCGACGTGGTGGCGCGCCTGAAGGC
AGAGAATCCCGATGCCCTTTTCATCTGCCACCCCGAATGCAACCCGGCGGTGGTGGCCCTGGCCGACCACGCCTGCTCCA
CGAGCGGCATGTACGATTACTGCCGCAAGAGTCCGGCCAAACGCTTCATCATCGGCACCGAGGCGGGAATCCTCTACAAG
CTCCGCCTGGAGAACCCCGACAAGGAGTTCATCCTCGCCTCGCCGGCCCTGGTCTGCCCCAACATGAAGCTCACCTCCCT
GGAGGACATCCTCGATGCCCTCACGACCATGGCGCCGGTGGTGCAGGTGCCTGAGGATATCCGGGTGCAAGCCAAGCGGG
CACTGGACCGGATGATCGCCATACCGAGGGACTGA

Upstream 100 bases:

>100_bases
CCGCCCATGGACGGGCAAAGGGTTTGACATCGGGGCGAACGTTGATTTAGTATCATCCAATTTCGCCCGTATACGGGAAA
ACGGGAAAGGAAACACACCC

Downstream 100 bases:

>100_bases
GATGCGGGGACCGGGGACCCGTGATCGGAAAAATCCAACCTGTCGAGGTCTATGATGATACGTTCTTTTCAGGGGATGAA
CCCCCGGATCGACTCCTCCG

Product: quinolinate synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MQADTIKQEIRRLLKERNAVLLAHNYMRDEVQEIADITGDSLGLSQEAAKTAADVIVFCGVHFMAESASILSPHKTVLLP
RRDAGCPMADMVTVEGLLELKARHPGVPVVTYVNSSAAVKAVSDICCTSANAVKVVNSLPDREVIFVPDRNLGQFVAKQS
DKAFHFWDGYCPTHERLKADVVARLKAENPDALFICHPECNPAVVALADHACSTSGMYDYCRKSPAKRFIIGTEAGILYK
LRLENPDKEFILASPALVCPNMKLTSLEDILDALTTMAPVVQVPEDIRVQAKRALDRMIAIPRD

Sequences:

>Translated_304_residues
MQADTIKQEIRRLLKERNAVLLAHNYMRDEVQEIADITGDSLGLSQEAAKTAADVIVFCGVHFMAESASILSPHKTVLLP
RRDAGCPMADMVTVEGLLELKARHPGVPVVTYVNSSAAVKAVSDICCTSANAVKVVNSLPDREVIFVPDRNLGQFVAKQS
DKAFHFWDGYCPTHERLKADVVARLKAENPDALFICHPECNPAVVALADHACSTSGMYDYCRKSPAKRFIIGTEAGILYK
LRLENPDKEFILASPALVCPNMKLTSLEDILDALTTMAPVVQVPEDIRVQAKRALDRMIAIPRD
>Mature_304_residues
MQADTIKQEIRRLLKERNAVLLAHNYMRDEVQEIADITGDSLGLSQEAAKTAADVIVFCGVHFMAESASILSPHKTVLLP
RRDAGCPMADMVTVEGLLELKARHPGVPVVTYVNSSAAVKAVSDICCTSANAVKVVNSLPDREVIFVPDRNLGQFVAKQS
DKAFHFWDGYCPTHERLKADVVARLKAENPDALFICHPECNPAVVALADHACSTSGMYDYCRKSPAKRFIIGTEAGILYK
LRLENPDKEFILASPALVCPNMKLTSLEDILDALTTMAPVVQVPEDIRVQAKRALDRMIAIPRD

Specific function: Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate

COG id: COG0379

COG function: function code H; Quinolinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the quinolinate synthase A family. Type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786964, Length=310, Percent_Identity=38.0645161290323, Blast_Score=197, Evalue=8e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADA_GEOMG (Q39PS5)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386474.1
- ProteinModelPortal:   Q39PS5
- SMR:   Q39PS5
- STRING:   Q39PS5
- GeneID:   3739803
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3544
- NMPDR:   fig|269799.3.peg.3512
- eggNOG:   COG0379
- HOGENOM:   HBG291157
- OMA:   CHPECNP
- PhylomeDB:   Q39PS5
- ProtClustDB:   PRK09375
- BioCyc:   GMET269799:GMET_3544-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00568
- InterPro:   IPR003473
- InterPro:   IPR023066
- TIGRFAMs:   TIGR00550

Pfam domain/function: PF02445 NadA

EC number: =2.5.1.72

Molecular weight: Translated: 33381; Mature: 33381

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 41-41 BINDING 112-112 BINDING 129-129 BINDING 197-197 BINDING 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQADTIKQEIRRLLKERNAVLLAHNYMRDEVQEIADITGDSLGLSQEAAKTAADVIVFCG
CCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VHFMAESASILSPHKTVLLPRRDAGCPMADMVTVEGLLELKARHPGVPVVTYVNSSAAVK
HHHHHCCCHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
AVSDICCTSANAVKVVNSLPDREVIFVPDRNLGQFVAKQSDKAFHFWDGYCPTHERLKAD
HHHHHHHCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHH
VVARLKAENPDALFICHPECNPAVVALADHACSTSGMYDYCRKSPAKRFIIGTEAGILYK
HHHHHCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCEEEE
LRLENPDKEFILASPALVCPNMKLTSLEDILDALTTMAPVVQVPEDIRVQAKRALDRMIA
EEECCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
IPRD
CCCC
>Mature Secondary Structure
MQADTIKQEIRRLLKERNAVLLAHNYMRDEVQEIADITGDSLGLSQEAAKTAADVIVFCG
CCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
VHFMAESASILSPHKTVLLPRRDAGCPMADMVTVEGLLELKARHPGVPVVTYVNSSAAVK
HHHHHCCCHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
AVSDICCTSANAVKVVNSLPDREVIFVPDRNLGQFVAKQSDKAFHFWDGYCPTHERLKAD
HHHHHHHCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHH
VVARLKAENPDALFICHPECNPAVVALADHACSTSGMYDYCRKSPAKRFIIGTEAGILYK
HHHHHCCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCEEEE
LRLENPDKEFILASPALVCPNMKLTSLEDILDALTTMAPVVQVPEDIRVQAKRALDRMIA
EEECCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
IPRD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA