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The map label for this gene is hofB [H]

Identifier: 78224591

GI number: 78224591

Start: 3815246

End: 3816868

Strand: Reverse

Name: hofB [H]

Synonym: Gmet_3401

Alternate gene names: 78224591

Gene position: 3816868-3815246 (Counterclockwise)

Preceding gene: 78224593

Following gene: 78224590

Centisome position: 95.48

GC content: 69.32

Gene sequence:

>1623_bases
ATGGAAAGCATCGTACGGCCTGGGTCTCTGGGGGACATACTCTTCCGGTGCCAGATCATCAGCGAGAACGATATCCGCGC
CGCCCTCGACGAGCAGCAGACGACGGGGTGCCGCTTCGGCGAGGCCCTGGTGAAACTGGGGGTCGTGGCCCAGGAGGACA
TCGACTGGGCCCTCTCCAACCAGCTCAACATCCCCTACGTACGGCTCAAACCGACCATGGTGGACACAGAGGCCGTGGCC
CTGATCCCGGCGGCACTGGCGCGCCAGCACAACCTTATCCCCCTCATCGTCACCGGGGACGAGATCAGCATCGCCATCGC
CGATCCCCTCAACACCGCGGCCCTGGCAGCGGTGGAGAAGGCTGCCGGCTGCCCCGTATCGGTGTCGGTGGGGCTCCTGC
GGGAGATCCGCGAGATGCAGGAGATCTTCTACGGCCCGCCGGAGGAGACCGACATCCTCGGCTTCGAGTCGGCCGCCTTC
CCGGCCAGCGTCCTCAGCGCCATCAACCACGACCTGACCGGCGCGAAGTTCGTCGATTACCTCCTCCTCTTCGTGGTCCA
GCAGAAACTCTCCTCCCTCTCCCTTCAGCCCCTGGGGGACGGGATCGTGGTGGTCTGCCGCCGGGGCGGGGTCGGACGCG
AAGCGGGGCGGCTCTCCCCCACCCATTACCCCGAGGTGGTCATGCGGGTGAAAAAGCTGGCCAAGCTGGCAGGAGGCGAA
TTCACCGCCAAGGGGGCATTCGCCTTCGGGTGGAAGGGGCGGATGATCCCGTTCCAGGTGGCGCTCCTGCGGGGGGAGGG
GGGCGATTACCTCACCTTCAGGATGCGGGTCGACGCCTCCTTCCCCGACTCCGTGGCCGACATGGGGCTTCCGGAGGCCA
CCACGGCCCGCTTTGCGGAACTGGCCGCGGCGGGGCGGGGGATGGTGGTCGTGGGCGCCCGGGAAGAGGAGATGCGGGGA
CGGCTCATGGAGCTGTACCTTCAGGAGTGCGACACCGCCGGCAAGACCGTCATCATGGTGGGGAGCGGCTCGCCCCAGGG
GGGGCGGCGCTTTCCCCGGGTGCCGGTGCCGGCCGGCACCGAAGTGGGAGCCGTGGTCATGGCAGCCCTCGAACACGACC
CCGACATCCTGATCCTGGAGGATGTGGCCGACGGCAAGGTCTTCGCGGCCGCCTGCCGGGCCGCCCAGCGGGGGACCCTC
GTGGTTGCGGGGTTCTCCTGCGGCGACGGGGCCGGTGCTCTGCAGCAGCTCATCACCTTCCGGGACCGGCACCTCCTCAC
CCCGGCCCATCTGCGGGGGATCATCGTCTGTGCCGGGGTCCGCACCCTCTGCCCCGACTGCCGTGCGGCCGTGCCGTCGC
CTCCCAACGAGGCGGAGCCCTCCTTCCGGTCGGCGGGGTGCCCGGCCTGCGGCCAGACCGGCTACGCGGGTACCCGCTTC
CTCATGGACATCATCGCCTTCGACCAGCGGGTGCGGGAGCTCTTCGAGTCCGCCCGCGACGCCGGCGACGTGCTCGGACA
CCTGCGGGAGCACGGCTGGCGGGGGATCGCCGAGGACGGACGGTCACTGGTCGCGCAGGGAGTAATCTCCCGGGAGGATT
TCATGGCCTCCCTACTCGGCTGA

Upstream 100 bases:

>100_bases
AGGGCCGTCTGTTGTGTAAAGGATGAGAACCGGGGCAAGCCGAAAATATTCCTCAAGAATTGAGCTGACCCGACCGATGT
AGTCAAAAGGGAGGATTACC

Downstream 100 bases:

>100_bases
CGGAGAAACCACATGGCACGCATCGACACACTCTTTAAAATGCTCAAGGATCAGGGGGCTTCCGACCTCCACCTCTCCTC
CGGGGCGCCCCCCATCTTCC

Product: general secretory system II, protein E-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MESIVRPGSLGDILFRCQIISENDIRAALDEQQTTGCRFGEALVKLGVVAQEDIDWALSNQLNIPYVRLKPTMVDTEAVA
LIPAALARQHNLIPLIVTGDEISIAIADPLNTAALAAVEKAAGCPVSVSVGLLREIREMQEIFYGPPEETDILGFESAAF
PASVLSAINHDLTGAKFVDYLLLFVVQQKLSSLSLQPLGDGIVVVCRRGGVGREAGRLSPTHYPEVVMRVKKLAKLAGGE
FTAKGAFAFGWKGRMIPFQVALLRGEGGDYLTFRMRVDASFPDSVADMGLPEATTARFAELAAAGRGMVVVGAREEEMRG
RLMELYLQECDTAGKTVIMVGSGSPQGGRRFPRVPVPAGTEVGAVVMAALEHDPDILILEDVADGKVFAAACRAAQRGTL
VVAGFSCGDGAGALQQLITFRDRHLLTPAHLRGIIVCAGVRTLCPDCRAAVPSPPNEAEPSFRSAGCPACGQTGYAGTRF
LMDIIAFDQRVRELFESARDAGDVLGHLREHGWRGIAEDGRSLVAQGVISREDFMASLLG

Sequences:

>Translated_540_residues
MESIVRPGSLGDILFRCQIISENDIRAALDEQQTTGCRFGEALVKLGVVAQEDIDWALSNQLNIPYVRLKPTMVDTEAVA
LIPAALARQHNLIPLIVTGDEISIAIADPLNTAALAAVEKAAGCPVSVSVGLLREIREMQEIFYGPPEETDILGFESAAF
PASVLSAINHDLTGAKFVDYLLLFVVQQKLSSLSLQPLGDGIVVVCRRGGVGREAGRLSPTHYPEVVMRVKKLAKLAGGE
FTAKGAFAFGWKGRMIPFQVALLRGEGGDYLTFRMRVDASFPDSVADMGLPEATTARFAELAAAGRGMVVVGAREEEMRG
RLMELYLQECDTAGKTVIMVGSGSPQGGRRFPRVPVPAGTEVGAVVMAALEHDPDILILEDVADGKVFAAACRAAQRGTL
VVAGFSCGDGAGALQQLITFRDRHLLTPAHLRGIIVCAGVRTLCPDCRAAVPSPPNEAEPSFRSAGCPACGQTGYAGTRF
LMDIIAFDQRVRELFESARDAGDVLGHLREHGWRGIAEDGRSLVAQGVISREDFMASLLG
>Mature_540_residues
MESIVRPGSLGDILFRCQIISENDIRAALDEQQTTGCRFGEALVKLGVVAQEDIDWALSNQLNIPYVRLKPTMVDTEAVA
LIPAALARQHNLIPLIVTGDEISIAIADPLNTAALAAVEKAAGCPVSVSVGLLREIREMQEIFYGPPEETDILGFESAAF
PASVLSAINHDLTGAKFVDYLLLFVVQQKLSSLSLQPLGDGIVVVCRRGGVGREAGRLSPTHYPEVVMRVKKLAKLAGGE
FTAKGAFAFGWKGRMIPFQVALLRGEGGDYLTFRMRVDASFPDSVADMGLPEATTARFAELAAAGRGMVVVGAREEEMRG
RLMELYLQECDTAGKTVIMVGSGSPQGGRRFPRVPVPAGTEVGAVVMAALEHDPDILILEDVADGKVFAAACRAAQRGTL
VVAGFSCGDGAGALQQLITFRDRHLLTPAHLRGIIVCAGVRTLCPDCRAAVPSPPNEAEPSFRSAGCPACGQTGYAGTRF
LMDIIAFDQRVRELFESARDAGDVLGHLREHGWRGIAEDGRSLVAQGVISREDFMASLLG

Specific function: Unknown

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=377, Percent_Identity=24.9336870026525, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789723, Length=422, Percent_Identity=21.3270142180095, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 57639; Mature: 57639

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIVRPGSLGDILFRCQIISENDIRAALDEQQTTGCRFGEALVKLGVVAQEDIDWALSN
CCCCCCCCCCCCEEEEEEEECCCCHHHHHCCHHCCCCHHHHHHHHHCCHHHCCCCHHHCC
QLNIPYVRLKPTMVDTEAVALIPAALARQHNLIPLIVTGDEISIAIADPLNTAALAAVEK
CCCCCEEEEECCEECCHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCHHHHHHHHH
AAGCPVSVSVGLLREIREMQEIFYGPPEETDILGFESAAFPASVLSAINHDLTGAKFVDY
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHCCCCCHHHHHHH
LLLFVVQQKLSSLSLQPLGDGIVVVCRRGGVGREAGRLSPTHYPEVVMRVKKLAKLAGGE
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
FTAKGAFAFGWKGRMIPFQVALLRGEGGDYLTFRMRVDASFPDSVADMGLPEATTARFAE
EEECCCEEECCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHCCCCCHHHHHHHH
LAAAGRGMVVVGAREEEMRGRLMELYLQECDTAGKTVIMVGSGSPQGGRRFPRVPVPAGT
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
EVGAVVMAALEHDPDILILEDVADGKVFAAACRAAQRGTLVVAGFSCGDGAGALQQLITF
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
RDRHLLTPAHLRGIIVCAGVRTLCPDCRAAVPSPPNEAEPSFRSAGCPACGQTGYAGTRF
HHCCCCCHHHHHHEEEECCHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LMDIIAFDQRVRELFESARDAGDVLGHLREHGWRGIAEDGRSLVAQGVISREDFMASLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MESIVRPGSLGDILFRCQIISENDIRAALDEQQTTGCRFGEALVKLGVVAQEDIDWALSN
CCCCCCCCCCCCEEEEEEEECCCCHHHHHCCHHCCCCHHHHHHHHHCCHHHCCCCHHHCC
QLNIPYVRLKPTMVDTEAVALIPAALARQHNLIPLIVTGDEISIAIADPLNTAALAAVEK
CCCCCEEEEECCEECCHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCHHHHHHHHH
AAGCPVSVSVGLLREIREMQEIFYGPPEETDILGFESAAFPASVLSAINHDLTGAKFVDY
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHCCCCCHHHHHHH
LLLFVVQQKLSSLSLQPLGDGIVVVCRRGGVGREAGRLSPTHYPEVVMRVKKLAKLAGGE
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
FTAKGAFAFGWKGRMIPFQVALLRGEGGDYLTFRMRVDASFPDSVADMGLPEATTARFAE
EEECCCEEECCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHCCCCCHHHHHHHH
LAAAGRGMVVVGAREEEMRGRLMELYLQECDTAGKTVIMVGSGSPQGGRRFPRVPVPAGT
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
EVGAVVMAALEHDPDILILEDVADGKVFAAACRAAQRGTLVVAGFSCGDGAGALQQLITF
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
RDRHLLTPAHLRGIIVCAGVRTLCPDCRAAVPSPPNEAEPSFRSAGCPACGQTGYAGTRF
HHCCCCCHHHHHHEEEECCHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LMDIIAFDQRVRELFESARDAGDVLGHLREHGWRGIAEDGRSLVAQGVISREDFMASLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959070; 8202364; 9278503 [H]