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The map label for this gene is pilT [H]

Identifier: 78224590

GI number: 78224590

Start: 3814163

End: 3815233

Strand: Reverse

Name: pilT [H]

Synonym: Gmet_3400

Alternate gene names: 78224590

Gene position: 3815233-3814163 (Counterclockwise)

Preceding gene: 78224591

Following gene: 78224589

Centisome position: 95.44

GC content: 64.61

Gene sequence:

>1071_bases
ATGGCACGCATCGACACACTCTTTAAAATGCTCAAGGATCAGGGGGCTTCCGACCTCCACCTCTCCTCCGGGGCGCCCCC
CATCTTCCGGCTCCACGGCGAGATGGCCCGCCAGAACTTCAAGCCTCTCACCCATGACGAGCTGAAGGCCATCCTCTACG
AGATCCTCACCGAGAAGCAGAAGGCCGACTTCGAGGAGCGCCACGACCTCGACTTCGCCTACGCCATCCCGGGGCTCGCC
CGGTTCCGGGGGAACTACATGCTGACCCACCGGGGGATCGCCGCGGTCTTCCGGATCATCCCCAGCAAGATCCTCTCGGC
CGACGATCTCAGCCTCCCCGACGGGGTCCGGCGCATGACCCTGTTCAAGAAGGGGCTCGTGCTCGTCACCGGCCCCACCG
GCTCGGGGAAATCCACCACCCTGGCCGCCATGATCGACCTCATCAACGCAACCCGCAAGGAGCACATCCTCACCCTGGAG
GACCCCCTCGAATTCATCCACGAGAACAAGATGTCGCTCCTGAACCAGCGGCAGATCGGCGAACACTCCATGACCTTCAC
CGCTGCCCTGCGGGCAGCCCTGCGGGAAGACCCCGACGTCATCCTCGTGGGGGAGATGCGGGACCTGGAGACCATCGGCC
TCGCCATGAGTGCCGCCGAGACCGGCCACCTGGTCTTCGGGACGCTGCACACCAACTCGGCGGCCAAGACCATCGACCGG
ATCATCGACGTCTTCCCCACCGACCAGCAGGAGCAGACCCGGGCCATGCTCTCCGAATCCCTCAAGGGGGTCGTCTGCCA
GCAGCTCCTGAAGACCGCCGACGGCAAGGGGCGGGTGGCGGCCCTGGAGATCATGCTCGGCACCCCGGCCATCGGCAACC
TGATCCGCGAGGGGAAGACCTTCCAGATCCCCTCCATCATCCAGACCGCCAAGCGGGACGGGATGCAGCTCATGGACCAG
CACCTCCTGGACCTCTTCAAGACCAAGCGGATCACCGCCGAAGAGGCCTACCGCTGCGCTCAGGACAAGAAACAGTTCGA
GCAGTATTTGGCCGAGAAGCCGGCCCAGTAA

Upstream 100 bases:

>100_bases
CTGGCGGGGGATCGCCGAGGACGGACGGTCACTGGTCGCGCAGGGAGTAATCTCCCGGGAGGATTTCATGGCCTCCCTAC
TCGGCTGACGGAGAAACCAC

Downstream 100 bases:

>100_bases
GGGGGACCGTGTTCCGGAAATAATGTGACGGTGTGGTTGTGCGGAAGGGCAGGGTTATTGAGATTGGTTTGATTTAACAA
TTGACCGGTACAGGCGATAT

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MARIDTLFKMLKDQGASDLHLSSGAPPIFRLHGEMARQNFKPLTHDELKAILYEILTEKQKADFEERHDLDFAYAIPGLA
RFRGNYMLTHRGIAAVFRIIPSKILSADDLSLPDGVRRMTLFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLE
DPLEFIHENKMSLLNQRQIGEHSMTFTAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDR
IIDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIGNLIREGKTFQIPSIIQTAKRDGMQLMDQ
HLLDLFKTKRITAEEAYRCAQDKKQFEQYLAEKPAQ

Sequences:

>Translated_356_residues
MARIDTLFKMLKDQGASDLHLSSGAPPIFRLHGEMARQNFKPLTHDELKAILYEILTEKQKADFEERHDLDFAYAIPGLA
RFRGNYMLTHRGIAAVFRIIPSKILSADDLSLPDGVRRMTLFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLE
DPLEFIHENKMSLLNQRQIGEHSMTFTAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDR
IIDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIGNLIREGKTFQIPSIIQTAKRDGMQLMDQ
HLLDLFKTKRITAEEAYRCAQDKKQFEQYLAEKPAQ
>Mature_355_residues
ARIDTLFKMLKDQGASDLHLSSGAPPIFRLHGEMARQNFKPLTHDELKAILYEILTEKQKADFEERHDLDFAYAIPGLAR
FRGNYMLTHRGIAAVFRIIPSKILSADDLSLPDGVRRMTLFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLED
PLEFIHENKMSLLNQRQIGEHSMTFTAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDRI
IDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIGNLIREGKTFQIPSIIQTAKRDGMQLMDQH
LLDLFKTKRITAEEAYRCAQDKKQFEQYLAEKPAQ

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=299, Percent_Identity=46.4882943143813, Blast_Score=270, Evalue=9e-74,
Organism=Escherichia coli, GI1789723, Length=148, Percent_Identity=45.945945945946, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786296, Length=149, Percent_Identity=46.9798657718121, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39709; Mature: 39577

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIDTLFKMLKDQGASDLHLSSGAPPIFRLHGEMARQNFKPLTHDELKAILYEILTEKQ
CCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KADFEERHDLDFAYAIPGLARFRGNYMLTHRGIAAVFRIIPSKILSADDLSLPDGVRRMT
HCCHHHHHCCCHHHHCCHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLEDPLEFIHENKMSLLNQRQIG
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHCC
EHSMTFTAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDR
CHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHH
IIDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIGNLIREGKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHCCCC
FQIPSIIQTAKRDGMQLMDQHLLDLFKTKRITAEEAYRCAQDKKQFEQYLAEKPAQ
EECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARIDTLFKMLKDQGASDLHLSSGAPPIFRLHGEMARQNFKPLTHDELKAILYEILTEKQ
CHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
KADFEERHDLDFAYAIPGLARFRGNYMLTHRGIAAVFRIIPSKILSADDLSLPDGVRRMT
HCCHHHHHCCCHHHHCCHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLEDPLEFIHENKMSLLNQRQIG
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHHHHHHCC
EHSMTFTAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDR
CHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHH
IIDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIGNLIREGKT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHCCCC
FQIPSIIQTAKRDGMQLMDQHLLDLFKTKRITAEEAYRCAQDKKQFEQYLAEKPAQ
EECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]