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The map label for this gene is hemL

Identifier: 78224546

GI number: 78224546

Start: 3772882

End: 3774165

Strand: Direct

Name: hemL

Synonym: Gmet_3356

Alternate gene names: 78224546

Gene position: 3772882-3774165 (Clockwise)

Preceding gene: 78224531

Following gene: 78224547

Centisome position: 94.38

GC content: 53.5

Gene sequence:

>1284_bases
ATGAACACATCCCATTCCAAAGAACTTTTTGCCAAGGCACAAGAAATTATCCCCGGCGGTGTTAACAGCCCAGTACGAGC
ATTCAAATCAGTAGGAGCAGAACCGGTTTTCATTAAGAAAGCTTCAGGCTCTACCATTATTGATGCCGACGATAATGCCT
ATATAGATTATGTTGGTTCCTGGGGACCGATGATTCTCGGCCATTGTCACCCCCGGATCGTGGCTTCCGTTCAGCGCGCC
ACGGAGAACGGCAGCAGCTTTGGTGCGCCGACCGAACTCGAAATCACTTTGGCGGAGATGGTGGTTGCAGCAGTCCCATC
GATCGAAATGGTCCGCATGGTAAGCTCAGGCACAGAAGCAACCATGAGCGCCATCCGCCTGGCACGCGGTTATACAGGAC
GAGACAAGATCATCAAATTTTCAGGATGTTACCATGGCCACGCCGACTCCCTCCTCGTCAAGGCAGGTTCCGGCGCGGCG
ACTTTTGGCGTTCCCGACTCACCTGGCGTTCCAAGGGACTTTGCTCAACATACCCTCACTGCAACCTTCAATGACCTTGA
ATCGGTCGAGGAACTCATTTGCGCCCATTCCAGAGAGGTCGCGTGCATTATTGTCGAGCCAATTGCTGGCAATATGGGTA
CCGTCCCTCCCCGCGCGGGATTTCTTGAAGGACTCCGAGAGATCTGCACCAACGAGGGAATTATCCTCATCTTCGACGAA
GTCATGACTGGTTTCCGCGTTGCATACGGCGGCGCCCAGGAACGCTATGGCATAACACCTGACATGACCACCTTGGGGAA
AATTATCGGCGGAGGCCTTCCTGTCGGAGCATTTGGTGGCAAAAGAGAGATAATGCAACAACTTTCGCCCTCGGGCGGGG
TTTATCAGGCAGGAACTCTTTCTGGCAATCCACTTGCCATGACCGCGGGCATAGAAACACTCAAGCTACTCCAGGAAGAC
GGCTTCTACGGTCGCCTCGAGGAAAAGAGTCGTGCGCTGGCCGAAGGGATTGCAGACGCAGCTCGTCGTGCAGGCTACCC
AATCTACTCGACACGGGTCGGCAGCATGTTTTGCGCTTTCTTCACAAACGGCGAAGTGCACGACTGGACGTCGGCGGCCA
TGTGCGACACCAAGTCTTTCGCCACCTTCTTCCGATCGATGCTTGAAGAAGGTATCTACCTGGCACCATCGCAGTTTGAA
ACCGCTTTTGTCTCCATTGCCCACTCCAGTGAAGACATCGAAAAGACCATTGCAGCGGCATTCTCATGTTTCAAAAAACT
CTAG

Upstream 100 bases:

>100_bases
CCCTGGCCGCAACCTTTTGCCCCTTGACACCTGACTTGCCACATGCTAATAACATATTAATTTTCCAGCTAAATTAGACA
CAATAAGGGGCCACACGACG

Downstream 100 bases:

>100_bases
CCGTTAATTGTCATTGACACCGTTATAGATGCTGTGGTAAGAAGCTGAGGTTTCCGTGCCACATTCCACTATACGTTGCC
CCATGGACGAAGAAAAACGA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNTSHSKELFAKAQEIIPGGVNSPVRAFKSVGAEPVFIKKASGSTIIDADDNAYIDYVGSWGPMILGHCHPRIVASVQRA
TENGSSFGAPTELEITLAEMVVAAVPSIEMVRMVSSGTEATMSAIRLARGYTGRDKIIKFSGCYHGHADSLLVKAGSGAA
TFGVPDSPGVPRDFAQHTLTATFNDLESVEELICAHSREVACIIVEPIAGNMGTVPPRAGFLEGLREICTNEGIILIFDE
VMTGFRVAYGGAQERYGITPDMTTLGKIIGGGLPVGAFGGKREIMQQLSPSGGVYQAGTLSGNPLAMTAGIETLKLLQED
GFYGRLEEKSRALAEGIADAARRAGYPIYSTRVGSMFCAFFTNGEVHDWTSAAMCDTKSFATFFRSMLEEGIYLAPSQFE
TAFVSIAHSSEDIEKTIAAAFSCFKKL

Sequences:

>Translated_427_residues
MNTSHSKELFAKAQEIIPGGVNSPVRAFKSVGAEPVFIKKASGSTIIDADDNAYIDYVGSWGPMILGHCHPRIVASVQRA
TENGSSFGAPTELEITLAEMVVAAVPSIEMVRMVSSGTEATMSAIRLARGYTGRDKIIKFSGCYHGHADSLLVKAGSGAA
TFGVPDSPGVPRDFAQHTLTATFNDLESVEELICAHSREVACIIVEPIAGNMGTVPPRAGFLEGLREICTNEGIILIFDE
VMTGFRVAYGGAQERYGITPDMTTLGKIIGGGLPVGAFGGKREIMQQLSPSGGVYQAGTLSGNPLAMTAGIETLKLLQED
GFYGRLEEKSRALAEGIADAARRAGYPIYSTRVGSMFCAFFTNGEVHDWTSAAMCDTKSFATFFRSMLEEGIYLAPSQFE
TAFVSIAHSSEDIEKTIAAAFSCFKKL
>Mature_427_residues
MNTSHSKELFAKAQEIIPGGVNSPVRAFKSVGAEPVFIKKASGSTIIDADDNAYIDYVGSWGPMILGHCHPRIVASVQRA
TENGSSFGAPTELEITLAEMVVAAVPSIEMVRMVSSGTEATMSAIRLARGYTGRDKIIKFSGCYHGHADSLLVKAGSGAA
TFGVPDSPGVPRDFAQHTLTATFNDLESVEELICAHSREVACIIVEPIAGNMGTVPPRAGFLEGLREICTNEGIILIFDE
VMTGFRVAYGGAQERYGITPDMTTLGKIIGGGLPVGAFGGKREIMQQLSPSGGVYQAGTLSGNPLAMTAGIETLKLLQED
GFYGRLEEKSRALAEGIADAARRAGYPIYSTRVGSMFCAFFTNGEVHDWTSAAMCDTKSFATFFRSMLEEGIYLAPSQFE
TAFVSIAHSSEDIEKTIAAAFSCFKKL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=356, Percent_Identity=27.5280898876405, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI37574042, Length=323, Percent_Identity=26.9349845201238, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI226442709, Length=290, Percent_Identity=27.9310344827586, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI24119277, Length=305, Percent_Identity=26.2295081967213, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI226442705, Length=321, Percent_Identity=24.6105919003115, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI284507298, Length=273, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=60.4705882352941, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1789016, Length=355, Percent_Identity=29.5774647887324, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787560, Length=351, Percent_Identity=29.9145299145299, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788044, Length=323, Percent_Identity=30.030959752322, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI145693181, Length=337, Percent_Identity=29.9703264094956, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1789759, Length=276, Percent_Identity=32.9710144927536, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786991, Length=328, Percent_Identity=29.8780487804878, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=343, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=342, Percent_Identity=28.6549707602339, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32564660, Length=341, Percent_Identity=28.7390029325513, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=254, Percent_Identity=28.740157480315, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323470, Length=308, Percent_Identity=29.5454545454545, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=319, Percent_Identity=25.3918495297806, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21357415, Length=298, Percent_Identity=27.5167785234899, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_GEOMG (Q39QA6)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386293.1
- ProteinModelPortal:   Q39QA6
- SMR:   Q39QA6
- STRING:   Q39QA6
- GeneID:   3739100
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3356
- NMPDR:   fig|269799.3.peg.3331
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   NENAVRD
- PhylomeDB:   Q39QA6
- ProtClustDB:   PRK00062
- BioCyc:   GMET269799:GMET_3356-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45479; Mature: 45479

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSHSKELFAKAQEIIPGGVNSPVRAFKSVGAEPVFIKKASGSTIIDADDNAYIDYVGS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEEEECC
WGPMILGHCHPRIVASVQRATENGSSFGAPTELEITLAEMVVAAVPSIEMVRMVSSGTEA
CCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHCCHHH
TMSAIRLARGYTGRDKIIKFSGCYHGHADSLLVKAGSGAATFGVPDSPGVPRDFAQHTLT
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHH
ATFNDLESVEELICAHSREVACIIVEPIAGNMGTVPPRAGFLEGLREICTNEGIILIFDE
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHH
VMTGFRVAYGGAQERYGITPDMTTLGKIIGGGLPVGAFGGKREIMQQLSPSGGVYQAGTL
HHHHHEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEECCC
SGNPLAMTAGIETLKLLQEDGFYGRLEEKSRALAEGIADAARRAGYPIYSTRVGSMFCAF
CCCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHEEE
FTNGEVHDWTSAAMCDTKSFATFFRSMLEEGIYLAPSQFETAFVSIAHSSEDIEKTIAAA
ECCCCCCCCCHHHHHCHHHHHHHHHHHHHCCEEECCCCHHHHHEEECCCHHHHHHHHHHH
FSCFKKL
HHHHHCC
>Mature Secondary Structure
MNTSHSKELFAKAQEIIPGGVNSPVRAFKSVGAEPVFIKKASGSTIIDADDNAYIDYVGS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEEEECC
WGPMILGHCHPRIVASVQRATENGSSFGAPTELEITLAEMVVAAVPSIEMVRMVSSGTEA
CCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHCCHHH
TMSAIRLARGYTGRDKIIKFSGCYHGHADSLLVKAGSGAATFGVPDSPGVPRDFAQHTLT
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHH
ATFNDLESVEELICAHSREVACIIVEPIAGNMGTVPPRAGFLEGLREICTNEGIILIFDE
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHH
VMTGFRVAYGGAQERYGITPDMTTLGKIIGGGLPVGAFGGKREIMQQLSPSGGVYQAGTL
HHHHHEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEECCC
SGNPLAMTAGIETLKLLQEDGFYGRLEEKSRALAEGIADAARRAGYPIYSTRVGSMFCAF
CCCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHEEE
FTNGEVHDWTSAAMCDTKSFATFFRSMLEEGIYLAPSQFETAFVSIAHSSEDIEKTIAAA
ECCCCCCCCCHHHHHCHHHHHHHHHHHHHCCEEECCCCHHHHHEEECCCHHHHHHHHHHH
FSCFKKL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA