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The map label for this gene is priA [H]

Identifier: 78224528

GI number: 78224528

Start: 3751107

End: 3753347

Strand: Direct

Name: priA [H]

Synonym: Gmet_3337

Alternate gene names: 78224528

Gene position: 3751107-3753347 (Clockwise)

Preceding gene: 78224515

Following gene: 78224529

Centisome position: 93.84

GC content: 65.19

Gene sequence:

>2241_bases
ATGAACCATTCCACGACGGACATCATTGAGGTTGCCATCCCGCTCCCCCTTGACACCACCTTCCACTACCGGGTGCCGGA
AGGGCTTATGCCGCGCATCGCCCCGGGAAAGCGGGTCCTGGTCCCCTTCGGCCGGCGGAAGGTGACCGGCTATATCCTCG
GCTTCGCGACCGGCGAGGAACGGGAACTGCGGGAGGTGGCAGACGTCCTGGATGACGCTCCCCTCTTCACGCCTCGGGAA
CTGGAGATCTACCGCTGGGCCGCTGCCTACTACCTCCATCCCCTGGGGGAGGTGATCAAGGCGGCTCTTCCTGCCGGAAT
CAATGTCCAGAGCCGGCGGGGCACCTCCACCGACGGCGATGCCATGACCGGGGGAAGGAGAGTGCTGACGGAGACCGTCT
ACCACCCCGCAACACCCCCTCCGGCTGTGGAGGTGCCGCGGGGAAAAGCATCCTCCATCCTCTCCCATATCCAGCAGGAA
GGGAAAGCCACGGCGGCGGAACTGCGCGGTATTTTTGCCGACTGCGCCCCCCAGCTACGCCGGCTCCGGGAGCTTGGCCT
CGTGGTGACGGAGGAGCGAGAGGTCTACCGGGACCCGTTCCGGGAGGAGTCCGTCACCCCCGACGCCCCCCTTCCCCTGA
ACCCTGGTCAAACCGAGGCCCGAGACCGCATCGTCGCAGCCATCGATACGGGGCGTTTTGCCCCCTTCCTCCTCCACGGC
GTCACCGGCAGCGGCAAGACCGAGGTCTATCTCCAGACCATCGCCCACCTCCTGGATGCCGGACGGACCGCCCTGGTCCT
CGTTCCGGAAATCGCCCTCACCCCCCAGCTCGTGAACCGCTTCCGGCGCCGGTTCCGGTGCGGCATCGCGGTGCTCCACA
GCGGCCTCTCCGACGGGGAGCGCTACGACGAGTGGCGACGGATCAGGCGAGGTGAAGTGGCCATCGTCATCGGTGCCCGT
TCGGCCATCTTTGCCCCCCTGGAGCGGATCGGCATGATCGTGGTGGATGAGGAGCACGAGTCCTCTTACAAGCAGTCGGA
GGGGTTCCGTTACAACGCACGGGACCTGGCCCTCGTACGGGGAAAGATGGAGAAAGCCTCAGTGGTCCTTGGGTCCGCCA
CCCCCCAGGTTACCACGTGGCATGCCGCCGTGACCGGGAAGCTCACCCTCCTCTCCCTTCCCGAACGGGTGAATGGCCTT
CCGGTCCCCCATGCCGAGATTATCGATGCCCGGGGGAAAAAAGCCGATGCAATACTCCCGCTGCTGGCCGATGCCACGGC
CGCCAACCTGGCCAACGGCGGGCAGACTTTGGTCTTTCTGAACCGCCGCGGCTTTGCCACCTGGCTCACCTGCGAGGAGT
GCGGCCACGTGCTCCGCTGCCCCAACTGCGCCGTCACCCTCACCTATCACCAGCGCAAGAACCGCCACGTCTGCCACTAC
TGCGACTACGCCATCCCGGCCCCTTCTGTCTGCCCCGACTGCGGAAGCACCCGGGTCACGCACCTCGGCCTTGGCACCGA
ACAGGTGGAAGAACTTGTGCGGGAACGCTTTCCCGAAGCCCGGGTCGACCGGATGGACCGGGACACAACGCGGGGGAGAG
GGGGCCACGCACGGATTTTGCGCAAGGTGGCCGAGCGGAAGACCGACATCCTCATTGGCACCCAGATGGTTGCCAAAGGG
CACGACTTCCCCGGCATCACCCTCGTGGGGATCGTGTCGGTGGAGTCAACTCTCAACATCCCCGACTACCGGAGCGCCGA
ACGGACCTATCAGCTGATGACACAGCTCATGGGGCGGGCCGGACGGGGCGAGGATGCGGGGAGGGTCATCATCCAGACCC
TCAATCCCGACCACTATGCTCTCATCCACGCCGGAACCGCATCCATGGAGGAGTTTTACACGACTGAGCTTGCCTTTCGT
CAGGAAACCGGCTATCCACCATTCGTTCACCTGGGGGCCATCTACCTGACCGGCACTGCTGCATCAGCCGTGGAACAGGA
GAGCAGGCGCCTTGCCGGACAGATCCGCACCCTGCGCCGTGAAGCGGGTGGACATGTCGAGATACTCGGCCCCTCCCCCG
CCCCCCTGCTGAAGCTGCGGGGGAGGTACCGGTGGCAGTTCCTACTCAAGGCACCGGTACGTTCGGCGCTCCACCGCCTC
CTTGCCCGTCTGCGGAGAACCTATACCCCTCCTTCCGGCATACGGATCCTGATCGATGTTGACCCGGTGGATCTCATGTG
A

Upstream 100 bases:

>100_bases
ACCGGTCTTGCCCAAGTCAAGACAGTGTGCCCCTCCCTTTCCCTTGTTCCCCCGGGACCGTCCTGCTATTATTCCCGTAA
CGCCATCAGCATCGGATCCC

Downstream 100 bases:

>100_bases
CTTCGCCGTCCCGTCAGGAAGGGTTTGATTTTTTCGCCCTATCTCTCTATATTCATACGAATAACTATCGTTGAAGGTGC
ATGAACCATGGTGCGTACAA

Product: primosomal protein n

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MNHSTTDIIEVAIPLPLDTTFHYRVPEGLMPRIAPGKRVLVPFGRRKVTGYILGFATGEERELREVADVLDDAPLFTPRE
LEIYRWAAAYYLHPLGEVIKAALPAGINVQSRRGTSTDGDAMTGGRRVLTETVYHPATPPPAVEVPRGKASSILSHIQQE
GKATAAELRGIFADCAPQLRRLRELGLVVTEEREVYRDPFREESVTPDAPLPLNPGQTEARDRIVAAIDTGRFAPFLLHG
VTGSGKTEVYLQTIAHLLDAGRTALVLVPEIALTPQLVNRFRRRFRCGIAVLHSGLSDGERYDEWRRIRRGEVAIVIGAR
SAIFAPLERIGMIVVDEEHESSYKQSEGFRYNARDLALVRGKMEKASVVLGSATPQVTTWHAAVTGKLTLLSLPERVNGL
PVPHAEIIDARGKKADAILPLLADATAANLANGGQTLVFLNRRGFATWLTCEECGHVLRCPNCAVTLTYHQRKNRHVCHY
CDYAIPAPSVCPDCGSTRVTHLGLGTEQVEELVRERFPEARVDRMDRDTTRGRGGHARILRKVAERKTDILIGTQMVAKG
HDFPGITLVGIVSVESTLNIPDYRSAERTYQLMTQLMGRAGRGEDAGRVIIQTLNPDHYALIHAGTASMEEFYTTELAFR
QETGYPPFVHLGAIYLTGTAASAVEQESRRLAGQIRTLRREAGGHVEILGPSPAPLLKLRGRYRWQFLLKAPVRSALHRL
LARLRRTYTPPSGIRILIDVDPVDLM

Sequences:

>Translated_746_residues
MNHSTTDIIEVAIPLPLDTTFHYRVPEGLMPRIAPGKRVLVPFGRRKVTGYILGFATGEERELREVADVLDDAPLFTPRE
LEIYRWAAAYYLHPLGEVIKAALPAGINVQSRRGTSTDGDAMTGGRRVLTETVYHPATPPPAVEVPRGKASSILSHIQQE
GKATAAELRGIFADCAPQLRRLRELGLVVTEEREVYRDPFREESVTPDAPLPLNPGQTEARDRIVAAIDTGRFAPFLLHG
VTGSGKTEVYLQTIAHLLDAGRTALVLVPEIALTPQLVNRFRRRFRCGIAVLHSGLSDGERYDEWRRIRRGEVAIVIGAR
SAIFAPLERIGMIVVDEEHESSYKQSEGFRYNARDLALVRGKMEKASVVLGSATPQVTTWHAAVTGKLTLLSLPERVNGL
PVPHAEIIDARGKKADAILPLLADATAANLANGGQTLVFLNRRGFATWLTCEECGHVLRCPNCAVTLTYHQRKNRHVCHY
CDYAIPAPSVCPDCGSTRVTHLGLGTEQVEELVRERFPEARVDRMDRDTTRGRGGHARILRKVAERKTDILIGTQMVAKG
HDFPGITLVGIVSVESTLNIPDYRSAERTYQLMTQLMGRAGRGEDAGRVIIQTLNPDHYALIHAGTASMEEFYTTELAFR
QETGYPPFVHLGAIYLTGTAASAVEQESRRLAGQIRTLRREAGGHVEILGPSPAPLLKLRGRYRWQFLLKAPVRSALHRL
LARLRRTYTPPSGIRILIDVDPVDLM
>Mature_746_residues
MNHSTTDIIEVAIPLPLDTTFHYRVPEGLMPRIAPGKRVLVPFGRRKVTGYILGFATGEERELREVADVLDDAPLFTPRE
LEIYRWAAAYYLHPLGEVIKAALPAGINVQSRRGTSTDGDAMTGGRRVLTETVYHPATPPPAVEVPRGKASSILSHIQQE
GKATAAELRGIFADCAPQLRRLRELGLVVTEEREVYRDPFREESVTPDAPLPLNPGQTEARDRIVAAIDTGRFAPFLLHG
VTGSGKTEVYLQTIAHLLDAGRTALVLVPEIALTPQLVNRFRRRFRCGIAVLHSGLSDGERYDEWRRIRRGEVAIVIGAR
SAIFAPLERIGMIVVDEEHESSYKQSEGFRYNARDLALVRGKMEKASVVLGSATPQVTTWHAAVTGKLTLLSLPERVNGL
PVPHAEIIDARGKKADAILPLLADATAANLANGGQTLVFLNRRGFATWLTCEECGHVLRCPNCAVTLTYHQRKNRHVCHY
CDYAIPAPSVCPDCGSTRVTHLGLGTEQVEELVRERFPEARVDRMDRDTTRGRGGHARILRKVAERKTDILIGTQMVAKG
HDFPGITLVGIVSVESTLNIPDYRSAERTYQLMTQLMGRAGRGEDAGRVIIQTLNPDHYALIHAGTASMEEFYTTELAFR
QETGYPPFVHLGAIYLTGTAASAVEQESRRLAGQIRTLRREAGGHVEILGPSPAPLLKLRGRYRWQFLLKAPVRSALHRL
LARLRRTYTPPSGIRILIDVDPVDLM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=754, Percent_Identity=38.7267904509284, Blast_Score=457, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82538; Mature: 82538

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSTTDIIEVAIPLPLDTTFHYRVPEGLMPRIAPGKRVLVPFGRRKVTGYILGFATGEE
CCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCEEEEECCCEEEEEEEEEECCCCH
RELREVADVLDDAPLFTPRELEIYRWAAAYYLHPLGEVIKAALPAGINVQSRRGTSTDGD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCC
AMTGGRRVLTETVYHPATPPPAVEVPRGKASSILSHIQQEGKATAAELRGIFADCAPQLR
CCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RLRELGLVVTEEREVYRDPFREESVTPDAPLPLNPGQTEARDRIVAAIDTGRFAPFLLHG
HHHHCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEE
VTGSGKTEVYLQTIAHLLDAGRTALVLVPEIALTPQLVNRFRRRFRCGIAVLHSGLSDGE
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCH
RYDEWRRIRRGEVAIVIGARSAIFAPLERIGMIVVDEEHESSYKQSEGFRYNARDLALVR
HHHHHHHHCCCCEEEEEECCHHHHHHHHHCCEEEEECCHHHHHHHCCCCEECCCHHHHHH
GKMEKASVVLGSATPQVTTWHAAVTGKLTLLSLPERVNGLPVPHAEIIDARGKKADAILP
CCCCHHEEEEECCCCCEEEEEEEEECCEEEEECHHHHCCCCCCCHHHHHCCCCCCCHHHH
LLADATAANLANGGQTLVFLNRRGFATWLTCEECGHVLRCPNCAVTLTYHQRKNRHVCHY
HHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHCCCEEECCCCEEEEEEECCCCCCEEEE
CDYAIPAPSVCPDCGSTRVTHLGLGTEQVEELVRERFPEARVDRMDRDTTRGRGGHARIL
ECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHH
RKVAERKTDILIGTQMVAKGHDFPGITLVGIVSVESTLNIPDYRSAERTYQLMTQLMGRA
HHHHHHCCCEEEEHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCC
GRGEDAGRVIIQTLNPDHYALIHAGTASMEEFYTTELAFRQETGYPPFVHLGAIYLTGTA
CCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEECEEEEECCH
ASAVEQESRRLAGQIRTLRREAGGHVEILGPSPAPLLKLRGRYRWQFLLKAPVRSALHRL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCEEEEEEEHHHHHHHHHHH
LARLRRTYTPPSGIRILIDVDPVDLM
HHHHHHHCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MNHSTTDIIEVAIPLPLDTTFHYRVPEGLMPRIAPGKRVLVPFGRRKVTGYILGFATGEE
CCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCEEEEECCCEEEEEEEEEECCCCH
RELREVADVLDDAPLFTPRELEIYRWAAAYYLHPLGEVIKAALPAGINVQSRRGTSTDGD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCC
AMTGGRRVLTETVYHPATPPPAVEVPRGKASSILSHIQQEGKATAAELRGIFADCAPQLR
CCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RLRELGLVVTEEREVYRDPFREESVTPDAPLPLNPGQTEARDRIVAAIDTGRFAPFLLHG
HHHHCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEE
VTGSGKTEVYLQTIAHLLDAGRTALVLVPEIALTPQLVNRFRRRFRCGIAVLHSGLSDGE
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCH
RYDEWRRIRRGEVAIVIGARSAIFAPLERIGMIVVDEEHESSYKQSEGFRYNARDLALVR
HHHHHHHHCCCCEEEEEECCHHHHHHHHHCCEEEEECCHHHHHHHCCCCEECCCHHHHHH
GKMEKASVVLGSATPQVTTWHAAVTGKLTLLSLPERVNGLPVPHAEIIDARGKKADAILP
CCCCHHEEEEECCCCCEEEEEEEEECCEEEEECHHHHCCCCCCCHHHHHCCCCCCCHHHH
LLADATAANLANGGQTLVFLNRRGFATWLTCEECGHVLRCPNCAVTLTYHQRKNRHVCHY
HHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHCCCEEECCCCEEEEEEECCCCCCEEEE
CDYAIPAPSVCPDCGSTRVTHLGLGTEQVEELVRERFPEARVDRMDRDTTRGRGGHARIL
ECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHH
RKVAERKTDILIGTQMVAKGHDFPGITLVGIVSVESTLNIPDYRSAERTYQLMTQLMGRA
HHHHHHCCCEEEEHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCC
GRGEDAGRVIIQTLNPDHYALIHAGTASMEEFYTTELAFRQETGYPPFVHLGAIYLTGTA
CCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEECEEEEECCH
ASAVEQESRRLAGQIRTLRREAGGHVEILGPSPAPLLKLRGRYRWQFLLKAPVRSALHRL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCEEEEEEEHHHHHHHHHHH
LARLRRTYTPPSGIRILIDVDPVDLM
HHHHHHHCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]