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The map label for this gene is hupD [H]

Identifier: 78224520

GI number: 78224520

Start: 3743266

End: 3743784

Strand: Reverse

Name: hupD [H]

Synonym: Gmet_3329

Alternate gene names: 78224520

Gene position: 3743784-3743266 (Counterclockwise)

Preceding gene: 78224521

Following gene: 78224519

Centisome position: 93.66

GC content: 66.47

Gene sequence:

>519_bases
ATGTCGATTCTCGTGCTCGGCATCGGGAACCTGATCATGACCGACGACGGCGCCGGCGTACGGGTGGCGCAGCGGCTCAT
GGACGGGTGCCGCTTCCCCGAAGGAGTGACGGTCATGGACGGCGGCACCCTCGGCCTCGACCTGCTCCCTTACCTGGACG
GCGTGGAGCGGCTCCTCCTGGTGGATGCCGTGGAGACCGGCGGACCACCGGGGACGGTGGTGCGGCTGGCGGGGGAGGAG
ATTCCGGTGGCCTTCCGGACCAAGCTCTCTCCCCACCAGATGGGGCTCCAGGATCTTTTGGCCGTGGCCGAGTTCCAGGG
GAGCTGTCCCGTCGAGATGGTCCTGTGGGGGGTGCAGCCGGAGTCGGTGGAACTGGGGATGGAGCTCACGCCGGCGGTGG
CGGCGCAGGTGGACCATCTTGTGGAGAAGGTCCTCGCGGAGCTTGCCCTGTGGGGAGTGGTTCCCGAGCCGCTTGATCCT
GAAAAATGCACTCGAAACACTGAGCAACAGAGCAACTGA

Upstream 100 bases:

>100_bases
CATCACATCTACAGCGCTTGGCTCATGGACGTGAAGGAACGGGGCGGGGTCATGTCGAGCATCTTCGGCGGCTACAAGCC
GGTGAAGCGGGGGGAGTAGA

Downstream 100 bases:

>100_bases
GCAAAATCTGGGAAAAACCTTTCAGACTGTTTTTGATAAATTCTCAGTTGCTCTGTGTTATTCATGCTGACGTTTTCATG
AGGTGAAGGGGCGTCCGGAC

Product: peptidase M52, hydrogenase maturation peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSILVLGIGNLIMTDDGAGVRVAQRLMDGCRFPEGVTVMDGGTLGLDLLPYLDGVERLLLVDAVETGGPPGTVVRLAGEE
IPVAFRTKLSPHQMGLQDLLAVAEFQGSCPVEMVLWGVQPESVELGMELTPAVAAQVDHLVEKVLAELALWGVVPEPLDP
EKCTRNTEQQSN

Sequences:

>Translated_172_residues
MSILVLGIGNLIMTDDGAGVRVAQRLMDGCRFPEGVTVMDGGTLGLDLLPYLDGVERLLLVDAVETGGPPGTVVRLAGEE
IPVAFRTKLSPHQMGLQDLLAVAEFQGSCPVEMVLWGVQPESVELGMELTPAVAAQVDHLVEKVLAELALWGVVPEPLDP
EKCTRNTEQQSN
>Mature_171_residues
SILVLGIGNLIMTDDGAGVRVAQRLMDGCRFPEGVTVMDGGTLGLDLLPYLDGVERLLLVDAVETGGPPGTVVRLAGEEI
PVAFRTKLSPHQMGLQDLLAVAEFQGSCPVEMVLWGVQPESVELGMELTPAVAAQVDHLVEKVLAELALWGVVPEPLDPE
KCTRNTEQQSN

Specific function: Not known. Could be involved in the processing of hydrogenase [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=154, Percent_Identity=42.8571428571429, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1787209, Length=163, Percent_Identity=39.8773006134969, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 18341; Mature: 18209

Theoretical pI: Translated: 4.00; Mature: 4.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILVLGIGNLIMTDDGAGVRVAQRLMDGCRFPEGVTVMDGGTLGLDLLPYLDGVERLLL
CEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
VDAVETGGPPGTVVRLAGEEIPVAFRTKLSPHQMGLQDLLAVAEFQGSCPVEMVLWGVQP
HHHHHCCCCCCEEEEECCCCCCEEEECCCCHHHHCHHHHHHHHHCCCCCCCEEEEECCCC
ESVELGMELTPAVAAQVDHLVEKVLAELALWGVVPEPLDPEKCTRNTEQQSN
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
SILVLGIGNLIMTDDGAGVRVAQRLMDGCRFPEGVTVMDGGTLGLDLLPYLDGVERLLL
EEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
VDAVETGGPPGTVVRLAGEEIPVAFRTKLSPHQMGLQDLLAVAEFQGSCPVEMVLWGVQP
HHHHHCCCCCCEEEEECCCCCCEEEECCCCHHHHCHHHHHHHHHCCCCCCCEEEEECCCC
ESVELGMELTPAVAAQVDHLVEKVLAELALWGVVPEPLDPEKCTRNTEQQSN
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]