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The map label for this gene is echA8 [H]

Identifier: 78224482

GI number: 78224482

Start: 3703790

End: 3704581

Strand: Direct

Name: echA8 [H]

Synonym: Gmet_3291

Alternate gene names: 78224482

Gene position: 3703790-3704581 (Clockwise)

Preceding gene: 78224481

Following gene: 78224483

Centisome position: 92.65

GC content: 68.81

Gene sequence:

>792_bases
ATGACCACTGAAACCATCCTGACCCAGATCGACGACCGGGGCGTGGCGACCCTCACCATGAACCGGCCCGAGCTCCACAA
CGCCTTTGACGATCTCCTCATCGCCGCCCTCACCGCGGAACTGCGGCGGCTCGAAGCGGACGACAGCGTGCGGGTCGTGA
TCCTCGCCGCCTCGGGCCGAAGCTTCTCCGCCGGGGCGGACCTCTCCTGGATGCGCCGCATGGCCGACTACACCCGGGAG
GAAAACCTTACCGACGCCCAGGTGCTGGCCGAGCTGATGCGGACCCTGGCCACCCTGAAGAAGCCGACTATCGCCCAGGT
CCAGGGGGCCGCCTTTGGCGGGGGGGTGGGGCTTGTGGCCTGCTGCGACGTGGCCATCGCCAGCCGGCGGGCCACCTTCT
GCCTCTCGGAGGTGAAACTGGGGCTCATCCCGGCGGTCATCTCCCCCTACGTGGTGGAGGCACTAGGTCCCCGGGCCGCG
CGCCGCTACTTCCAGAGCGCCGAGATATTCGACGCCGCCGAGGCCCGCGGCCTCGGTCTCGTCCACGAGGTGACGACGGA
GGAGGAGCTTCCCGCCGCCGTGGAACGCCTTGCCGCAACTCTTCTCCTCAACGGTCCCCGGGCCATGGCCGCCGCCAAGG
AGCTCGTGGCCCGGGTCGCCTCCGGCCCCATCGACGAGGCGATGATACGCGACACCGCCGAACGGATCGCCGACACCCGC
GCCTCCGCCGAGGGGAAAGAGGGGCTCTCCGCCTTCCTGGAAAAAAGAAAACCTCGCTGGGTGAAAGGATAG

Upstream 100 bases:

>100_bases
GCTTGTGCGACGTAGCGCTGCTACGCCTCCGCGCAATTCCTAGATCGCCTTGACCTTGCGAAAAATCCACGTTCACCGGA
AGACCAAAGGACACAGAACA

Downstream 100 bases:

>100_bases
ATACCATGTTCGATACGATTCTCATTGCAAACCGGGGCGAAATTGCCTGCCGCGTCATCAGCACCGCCCGCCGCCTCGGC
ATCCGCACCGTGGCCGTCTA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTTETILTQIDDRGVATLTMNRPELHNAFDDLLIAALTAELRRLEADDSVRVVILAASGRSFSAGADLSWMRRMADYTRE
ENLTDAQVLAELMRTLATLKKPTIAQVQGAAFGGGVGLVACCDVAIASRRATFCLSEVKLGLIPAVISPYVVEALGPRAA
RRYFQSAEIFDAAEARGLGLVHEVTTEEELPAAVERLAATLLLNGPRAMAAAKELVARVASGPIDEAMIRDTAERIADTR
ASAEGKEGLSAFLEKRKPRWVKG

Sequences:

>Translated_263_residues
MTTETILTQIDDRGVATLTMNRPELHNAFDDLLIAALTAELRRLEADDSVRVVILAASGRSFSAGADLSWMRRMADYTRE
ENLTDAQVLAELMRTLATLKKPTIAQVQGAAFGGGVGLVACCDVAIASRRATFCLSEVKLGLIPAVISPYVVEALGPRAA
RRYFQSAEIFDAAEARGLGLVHEVTTEEELPAAVERLAATLLLNGPRAMAAAKELVARVASGPIDEAMIRDTAERIADTR
ASAEGKEGLSAFLEKRKPRWVKG
>Mature_262_residues
TTETILTQIDDRGVATLTMNRPELHNAFDDLLIAALTAELRRLEADDSVRVVILAASGRSFSAGADLSWMRRMADYTREE
NLTDAQVLAELMRTLATLKKPTIAQVQGAAFGGGVGLVACCDVAIASRRATFCLSEVKLGLIPAVISPYVVEALGPRAAR
RYFQSAEIFDAAEARGLGLVHEVTTEEELPAAVERLAATLLLNGPRAMAAAKELVARVASGPIDEAMIRDTAERIADTRA
SAEGKEGLSAFLEKRKPRWVKG

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=30, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI31542718, Length=259, Percent_Identity=26.6409266409266, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4502327, Length=263, Percent_Identity=31.1787072243346, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI213417737, Length=205, Percent_Identity=25.3658536585366, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI157694516, Length=205, Percent_Identity=25.3658536585366, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI221136756, Length=240, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221136753, Length=240, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=29.8165137614679, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=29.8165137614679, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221307494, Length=240, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=29.8165137614679, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI70995211, Length=272, Percent_Identity=25.7352941176471, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=29.8342541436464, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=32.824427480916, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=31.9838056680162, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=28.3524904214559, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=30.7392996108949, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082183, Length=250, Percent_Identity=29.2, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=30.3149606299213, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=30.0395256916996, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=204, Percent_Identity=31.3725490196078, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=181, Percent_Identity=28.7292817679558, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=246, Percent_Identity=23.5772357723577, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24653139, Length=273, Percent_Identity=32.2344322344322, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129971, Length=264, Percent_Identity=28.030303030303, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653477, Length=264, Percent_Identity=28.030303030303, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=22.7091633466135, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19921018, Length=203, Percent_Identity=31.0344827586207, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24583165, Length=206, Percent_Identity=29.126213592233, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28279; Mature: 28148

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTETILTQIDDRGVATLTMNRPELHNAFDDLLIAALTAELRRLEADDSVRVVILAASGR
CCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
SFSAGADLSWMRRMADYTREENLTDAQVLAELMRTLATLKKPTIAQVQGAAFGGGVGLVA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHH
CCDVAIASRRATFCLSEVKLGLIPAVISPYVVEALGPRAARRYFQSAEIFDAAEARGLGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCH
VHEVTTEEELPAAVERLAATLLLNGPRAMAAAKELVARVASGPIDEAMIRDTAERIADTR
HHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ASAEGKEGLSAFLEKRKPRWVKG
HCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTETILTQIDDRGVATLTMNRPELHNAFDDLLIAALTAELRRLEADDSVRVVILAASGR
CCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
SFSAGADLSWMRRMADYTREENLTDAQVLAELMRTLATLKKPTIAQVQGAAFGGGVGLVA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHH
CCDVAIASRRATFCLSEVKLGLIPAVISPYVVEALGPRAARRYFQSAEIFDAAEARGLGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCH
VHEVTTEEELPAAVERLAATLLLNGPRAMAAAKELVARVASGPIDEAMIRDTAERIADTR
HHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ASAEGKEGLSAFLEKRKPRWVKG
HCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]