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The map label for this gene is yqiR [H]

Identifier: 78224476

GI number: 78224476

Start: 3694914

End: 3696743

Strand: Direct

Name: yqiR [H]

Synonym: Gmet_3285

Alternate gene names: 78224476

Gene position: 3694914-3696743 (Clockwise)

Preceding gene: 78224475

Following gene: 78224477

Centisome position: 92.43

GC content: 55.03

Gene sequence:

>1830_bases
ATGCAGGCAGGTGAACCTCGAAACGGAAAACAGGTTGTCATGGATCAAGAGGAAATAATGGAGAGAAACGGCGCTTCTGA
AAATATGCACAAAAAGGCCGGGGAGGCTGGAATTATCCGACTTGACGCCGAACTGGGGATCGTCTCCCTGGACGAAACCT
CCGGAAGCCTGATCGGAAGCCCTCCCGAATCTCTTCTGGGACGGAGAATCGACGGGATAGTCGACTTGGCGAACCTGGGC
AAACTGATGCACAACGGCATCAGCTTCAGCAATCAGGTCATCGTTGCCGGTGACCGGCGTCTCGCCTGCGATTACGTCCC
CATCGTGGAGGATGACAGGATTGTCGGCGGAGTATTGACGGTACTCGGCGTACTCCCCAAGGAGACCGACGGCTCTTCGG
ACGACCTCAAGGAACTTCTTCGCTCTGCCGGCGCTTTCATGGACCTCGACTATCACGGCATCATCATCCTCGACCGCAAC
GGCACGGTCGTAATGGTAAACCAGTCCTTTGCCGAGGTGCTCGACGCCACCCCGCAGACCATGATCGGCAAGCATGTCCA
TCAGGCTTATCATAATTCACAACCCTCCCGGATGCCGGTCGTGATGGAAACCGGCAAACCGGAGATCAGCTCCCACTACA
TGAACGGCAAAGAGGTCTTTGCCAGCCGCTACCCTCTTATCAAGCAGGGGAAAGTGATCGGTTGCGTGGGAAAGATTCTT
TTCAAGGATGTCCGCGAGATCTCACTCCTCGCGAATCGGCTGCAGAGTGCCCGAGAGAAAAAAACTCCCGCGAGAGCAAT
TGCGGAAAAAGGTTCCCTTTTCAAATACGACATTGACAGCATTGTGGGGCAGAGCGCCAAAATCGCCGAACTGAAGGAGA
CCCTTCTCAGGGTAGCCCCCAAGAACTCCAACGTTCTCTTGAGGGGAGAGAGCGGGACGGGGAAGGAACTCTGCGCCCAT
GCCATCCACGCTGCCAGCACACGACGCTACGCCCCCTTCGTCAAGGTGAACTGCGCCGCCATCCCTGACCACCTCCTGGA
ATCGGAACTCTTCGGTTACGCGGACGGGGCCTTTACCGGGGCCAAAAAAGGAGGGCAGGTCGGCAAGTTCGAGCTGGCCG
ACACCGGCACCATCTTTCTTGACGAGATCGGTGACATGCCCCTTGCCATGCAGGCAAAACTTCTCCGAGTTCTTCAAGAG
CGGGAACTGGTCCCCTTGGGAAGCACCGTCCCAAAGGTAGTTGATGTGCGAGTCGTGGCGGCAACCAACAGCAACCTCGA
ACAGTTGGTCAGCGAAGGGAAATTCAGGGAGGACCTCTATTATCGGCTTAATGTGGTCGCCCTTACCATTCCTGCGCTGA
AGGACAGAATGGAAGACATCTACATCATCACGAAAAATTTCATTGATCATTTCAACGCTGAATTCGGCCTCTCCGTCCAG
GGAATCGACAGTGAAGCGTGGGAGGTCCTCAAGCACCACAACTGGCCCGGCAATATCCGCGAGCTTCGCAACGTGCTCGA
AAGCGCCTTCAATGCAGTAAGCGGCCCTCTCATCACCAAAGAAAACCTCCCAATCCGTCTCGCCCAACTCTGCCCACATT
CCACGCTGCCCCATGAGTTTGCGCCTGGAGAGGATCTGGAAAGTTACATTCGACAGCACCTGGGAAAGAAAAACATTAGT
GATATGATGGACGACCTTGAACGGGTAATCCTGGAAAAAGCCCTTGAAATCTGCAACGGCAACAAGCTCCACGCCGCTCA
ACTCCTCGGCATATCCCGCCCAGGTCTCTACAAAAAGCTCCAGAAGTACCCCTCACTGGAAAAAACATAA

Upstream 100 bases:

>100_bases
GATTTCCTCGATCTGTGCGCCTGTTTCCAAGGGATGGCGCACCATACCGACGATCACCGAGAGGCCATCAATGCCTTCCT
CGAAAAAAGGGCTCCCGTCT

Downstream 100 bases:

>100_bases
GACGTATATGAGTATACCGTCGCCGAATTGCCATTCAATGGCCATACAATAACGTTCTATTCATTTCACACATCCGAAGC
CCTACATTTCAAACCTTTTC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MQAGEPRNGKQVVMDQEEIMERNGASENMHKKAGEAGIIRLDAELGIVSLDETSGSLIGSPPESLLGRRIDGIVDLANLG
KLMHNGISFSNQVIVAGDRRLACDYVPIVEDDRIVGGVLTVLGVLPKETDGSSDDLKELLRSAGAFMDLDYHGIIILDRN
GTVVMVNQSFAEVLDATPQTMIGKHVHQAYHNSQPSRMPVVMETGKPEISSHYMNGKEVFASRYPLIKQGKVIGCVGKIL
FKDVREISLLANRLQSAREKKTPARAIAEKGSLFKYDIDSIVGQSAKIAELKETLLRVAPKNSNVLLRGESGTGKELCAH
AIHAASTRRYAPFVKVNCAAIPDHLLESELFGYADGAFTGAKKGGQVGKFELADTGTIFLDEIGDMPLAMQAKLLRVLQE
RELVPLGSTVPKVVDVRVVAATNSNLEQLVSEGKFREDLYYRLNVVALTIPALKDRMEDIYIITKNFIDHFNAEFGLSVQ
GIDSEAWEVLKHHNWPGNIRELRNVLESAFNAVSGPLITKENLPIRLAQLCPHSTLPHEFAPGEDLESYIRQHLGKKNIS
DMMDDLERVILEKALEICNGNKLHAAQLLGISRPGLYKKLQKYPSLEKT

Sequences:

>Translated_609_residues
MQAGEPRNGKQVVMDQEEIMERNGASENMHKKAGEAGIIRLDAELGIVSLDETSGSLIGSPPESLLGRRIDGIVDLANLG
KLMHNGISFSNQVIVAGDRRLACDYVPIVEDDRIVGGVLTVLGVLPKETDGSSDDLKELLRSAGAFMDLDYHGIIILDRN
GTVVMVNQSFAEVLDATPQTMIGKHVHQAYHNSQPSRMPVVMETGKPEISSHYMNGKEVFASRYPLIKQGKVIGCVGKIL
FKDVREISLLANRLQSAREKKTPARAIAEKGSLFKYDIDSIVGQSAKIAELKETLLRVAPKNSNVLLRGESGTGKELCAH
AIHAASTRRYAPFVKVNCAAIPDHLLESELFGYADGAFTGAKKGGQVGKFELADTGTIFLDEIGDMPLAMQAKLLRVLQE
RELVPLGSTVPKVVDVRVVAATNSNLEQLVSEGKFREDLYYRLNVVALTIPALKDRMEDIYIITKNFIDHFNAEFGLSVQ
GIDSEAWEVLKHHNWPGNIRELRNVLESAFNAVSGPLITKENLPIRLAQLCPHSTLPHEFAPGEDLESYIRQHLGKKNIS
DMMDDLERVILEKALEICNGNKLHAAQLLGISRPGLYKKLQKYPSLEKT
>Mature_609_residues
MQAGEPRNGKQVVMDQEEIMERNGASENMHKKAGEAGIIRLDAELGIVSLDETSGSLIGSPPESLLGRRIDGIVDLANLG
KLMHNGISFSNQVIVAGDRRLACDYVPIVEDDRIVGGVLTVLGVLPKETDGSSDDLKELLRSAGAFMDLDYHGIIILDRN
GTVVMVNQSFAEVLDATPQTMIGKHVHQAYHNSQPSRMPVVMETGKPEISSHYMNGKEVFASRYPLIKQGKVIGCVGKIL
FKDVREISLLANRLQSAREKKTPARAIAEKGSLFKYDIDSIVGQSAKIAELKETLLRVAPKNSNVLLRGESGTGKELCAH
AIHAASTRRYAPFVKVNCAAIPDHLLESELFGYADGAFTGAKKGGQVGKFELADTGTIFLDEIGDMPLAMQAKLLRVLQE
RELVPLGSTVPKVVDVRVVAATNSNLEQLVSEGKFREDLYYRLNVVALTIPALKDRMEDIYIITKNFIDHFNAEFGLSVQ
GIDSEAWEVLKHHNWPGNIRELRNVLESAFNAVSGPLITKENLPIRLAQLCPHSTLPHEFAPGEDLESYIRQHLGKKNIS
DMMDDLERVILEKALEICNGNKLHAAQLLGISRPGLYKKLQKYPSLEKT

Specific function: Unknown

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789233, Length=526, Percent_Identity=34.9809885931559, Blast_Score=288, Evalue=7e-79,
Organism=Escherichia coli, GI1788550, Length=362, Percent_Identity=42.8176795580111, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1790437, Length=322, Percent_Identity=42.8571428571429, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1786524, Length=346, Percent_Identity=39.3063583815029, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1790299, Length=372, Percent_Identity=39.5161290322581, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI87082117, Length=333, Percent_Identity=44.4444444444444, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788905, Length=330, Percent_Identity=40, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1789087, Length=277, Percent_Identity=44.043321299639, Blast_Score=220, Evalue=3e-58,
Organism=Escherichia coli, GI87082152, Length=229, Percent_Identity=46.7248908296943, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=43.9655172413793, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=41.1764705882353, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1789828, Length=356, Percent_Identity=32.3033707865169, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87081858, Length=299, Percent_Identity=30.4347826086957, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR002197
- InterPro:   IPR016040
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 66978; Mature: 66978

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50112 PAS ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAGEPRNGKQVVMDQEEIMERNGASENMHKKAGEAGIIRLDAELGIVSLDETSGSLIGS
CCCCCCCCCCEEEECHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCC
PPESLLGRRIDGIVDLANLGKLMHNGISFSNQVIVAGDRRLACDYVPIVEDDRIVGGVLT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCCHHHHHHH
VLGVLPKETDGSSDDLKELLRSAGAFMDLDYHGIIILDRNGTVVMVNQSFAEVLDATPQT
HHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEECCCCEEEECCHHHHHHCCCHHH
MIGKHVHQAYHNSQPSRMPVVMETGKPEISSHYMNGKEVFASRYPLIKQGKVIGCVGKIL
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHCCHHHHHHHCCCCHHCCCCHHHHHHHH
FKDVREISLLANRLQSAREKKTPARAIAEKGSLFKYDIDSIVGQSAKIAELKETLLRVAP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHCC
KNSNVLLRGESGTGKELCAHAIHAASTRRYAPFVKVNCAAIPDHLLESELFGYADGAFTG
CCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCC
AKKGGQVGKFELADTGTIFLDEIGDMPLAMQAKLLRVLQERELVPLGSTVPKVVDVRVVA
CCCCCCCCEEEECCCCEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEE
ATNSNLEQLVSEGKFREDLYYRLNVVALTIPALKDRMEDIYIITKNFIDHFNAEFGLSVQ
ECCCCHHHHHHCCCHHHHHHHEEEEEEEEEHHHHHHHHHEEEEHHHHHHHCCCCCCCEEE
GIDSEAWEVLKHHNWPGNIRELRNVLESAFNAVSGPLITKENLPIRLAQLCPHSTLPHEF
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCCCCC
APGEDLESYIRQHLGKKNISDMMDDLERVILEKALEICNGNKLHAAQLLGISRPGLYKKL
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCHHHHHH
QKYPSLEKT
HHCCCCCCC
>Mature Secondary Structure
MQAGEPRNGKQVVMDQEEIMERNGASENMHKKAGEAGIIRLDAELGIVSLDETSGSLIGS
CCCCCCCCCCEEEECHHHHHHHCCCCCHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCC
PPESLLGRRIDGIVDLANLGKLMHNGISFSNQVIVAGDRRLACDYVPIVEDDRIVGGVLT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCCHHHHHHH
VLGVLPKETDGSSDDLKELLRSAGAFMDLDYHGIIILDRNGTVVMVNQSFAEVLDATPQT
HHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEECCCCEEEECCHHHHHHCCCHHH
MIGKHVHQAYHNSQPSRMPVVMETGKPEISSHYMNGKEVFASRYPLIKQGKVIGCVGKIL
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHCCHHHHHHHCCCCHHCCCCHHHHHHHH
FKDVREISLLANRLQSAREKKTPARAIAEKGSLFKYDIDSIVGQSAKIAELKETLLRVAP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEHHHHHCCCCHHHHHHHHHHHHCC
KNSNVLLRGESGTGKELCAHAIHAASTRRYAPFVKVNCAAIPDHLLESELFGYADGAFTG
CCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCC
AKKGGQVGKFELADTGTIFLDEIGDMPLAMQAKLLRVLQERELVPLGSTVPKVVDVRVVA
CCCCCCCCEEEECCCCEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEE
ATNSNLEQLVSEGKFREDLYYRLNVVALTIPALKDRMEDIYIITKNFIDHFNAEFGLSVQ
ECCCCHHHHHHCCCHHHHHHHEEEEEEEEEHHHHHHHHHEEEEHHHHHHHCCCCCCCEEE
GIDSEAWEVLKHHNWPGNIRELRNVLESAFNAVSGPLITKENLPIRLAQLCPHSTLPHEF
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHCCCCCCCCCC
APGEDLESYIRQHLGKKNISDMMDDLERVILEKALEICNGNKLHAAQLLGISRPGLYKKL
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCHHHHHH
QKYPSLEKT
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]