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The map label for this gene is istB [H]

Identifier: 78224472

GI number: 78224472

Start: 3690629

End: 3691423

Strand: Reverse

Name: istB [H]

Synonym: Gmet_3281

Alternate gene names: 78224472

Gene position: 3691423-3690629 (Counterclockwise)

Preceding gene: 78224473

Following gene: 78224471

Centisome position: 92.35

GC content: 58.49

Gene sequence:

>795_bases
ATGAGCGACATCCAGCACCAGCGTATGGTCACCCTCTGCGACGACTTGAAGTTTCTGGCGGTGACCGATGTGTACGCCGA
CCTTGCCGATGCTGCGGCAAAGCAGGAGTCGTCATACATCGACTATCTCGAGCAGGTACTCAAGGCCGAGAACGACGTCC
GGCAGGGGCGCTCGCGCCACACCATGGCAAAGCTCGCGGGCTTTCCCGCGATCAAGACCCTTGAGGATTATGACTTCGAT
TTTGCCACCGGCGCCCCGAAGCAGCGAATCCTGGACCTATCGGCAATGGCGTTTCTGGAGCGTCGGGAGAATGTAATCCT
GCTCGGTCCCAGCGGTACCGGCAAGACTCACCTCGCCATTGCCCTCGGCTACCGGGCAACCCAGTGCGGCGTGAAGGTGC
GCTTCATTTCCGCTGCGGACCTGATGCTGCAACTCGAAAGCGCCCAGCGCCAGGGGCGGTACAAGGAAGTAATGCGGCGC
AGTGTCCTGGGGCCGAGACTACTCATCATCGACGAGATTGGATACCTTCCGTTCAGCGAAACACAGGCAAACCTGTTCTT
TCAGGTGATCGCCAAAAGGTACGAAACAGGTTCCGTCATCCTCACCTCGAACCTGAGCTTTGGGGAATGGGAACAGGCTT
TCGGCGGCAACACGGCACTCACATCAGCAATGCTCGACCGGTTGCTGCACCACGCCCATGTCATTCAGATCAGGGGTGAC
AGCTACCGATTGAAAGAGAAGCGCCGAGCTGGCATTCTCGGGCAGCAACTGCCGACACCCCAGATGGATGATTAA

Upstream 100 bases:

>100_bases
AGCAGGGCAAGCCGGAAGTCATCACTCCCATGCCGTGGCCGGTGATCCCACTTCAGCGCCCCGCCTCCTTCTATGATCAG
ATCCTTCAGGAGGGGCGGCT

Downstream 100 bases:

>100_bases
CCCAGGAAGGTGGGTCATTTTTCAATTGCCGATCAACCGGTAAAGTGGGTCAATTTTCGATTGCCGTTGACAACTGGTAC
TTCACCATGGCCATTGCGGC

Product: transposase/IS protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSDIQHQRMVTLCDDLKFLAVTDVYADLADAAAKQESSYIDYLEQVLKAENDVRQGRSRHTMAKLAGFPAIKTLEDYDFD
FATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAIALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRR
SVLGPRLLIIDEIGYLPFSETQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGD
SYRLKEKRRAGILGQQLPTPQMDD

Sequences:

>Translated_264_residues
MSDIQHQRMVTLCDDLKFLAVTDVYADLADAAAKQESSYIDYLEQVLKAENDVRQGRSRHTMAKLAGFPAIKTLEDYDFD
FATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAIALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRR
SVLGPRLLIIDEIGYLPFSETQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGD
SYRLKEKRRAGILGQQLPTPQMDD
>Mature_263_residues
SDIQHQRMVTLCDDLKFLAVTDVYADLADAAAKQESSYIDYLEQVLKAENDVRQGRSRHTMAKLAGFPAIKTLEDYDFDF
ATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAIALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRRS
VLGPRLLIIDEIGYLPFSETQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGDS
YRLKEKRRAGILGQQLPTPQMDD

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 29615; Mature: 29484

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIQHQRMVTLCDDLKFLAVTDVYADLADAAAKQESSYIDYLEQVLKAENDVRQGRSRH
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
TMAKLAGFPAIKTLEDYDFDFATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAI
HHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEE
ALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRRSVLGPRLLIIDEIGYLPFSE
EECCEEHHCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCC
TQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGD
HHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHEEEEECCC
SYRLKEKRRAGILGQQLPTPQMDD
CCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDIQHQRMVTLCDDLKFLAVTDVYADLADAAAKQESSYIDYLEQVLKAENDVRQGRSRH
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
TMAKLAGFPAIKTLEDYDFDFATGAPKQRILDLSAMAFLERRENVILLGPSGTGKTHLAI
HHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEE
ALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRRSVLGPRLLIIDEIGYLPFSE
EECCEEHHCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCCCCCC
TQANLFFQVIAKRYETGSVILTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGD
HHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHEEEEECCC
SYRLKEKRRAGILGQQLPTPQMDD
CCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]