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The map label for this gene is purA

Identifier: 78224451

GI number: 78224451

Start: 3668920

End: 3670212

Strand: Direct

Name: purA

Synonym: Gmet_3260

Alternate gene names: 78224451

Gene position: 3668920-3670212 (Clockwise)

Preceding gene: 78224450

Following gene: 78224455

Centisome position: 91.78

GC content: 60.87

Gene sequence:

>1293_bases
ATGGCAAACGTGGTAGTGGTTGGCGCCCAGTGGGGCGATGAAGGAAAAGGCAAGGTTGTCGATATCTACACCGAGCATGC
AGACGACGTGGTCCGCTACCAGGGAGGAAACAACGCCGGCCATACGCTGGTCGTAAACGGCGAGAAGACCGTCCTCCACC
TCATTCCGTCCGGGATTCTCCACAAGGGAAAGCGGTGCATCATCGGCAACGGCGTGGTGCTCGACCCCGAGGTCTTCATC
ATGGAGATCAACCGACTGAAGGAAAAGGGTCGCCTCCAGGACGACAGCGCCCTTTTGGTCAGCGAATCGGTCCACATCAT
TATGCCGTATCACAAGCAGATCGACCTGGCCCGTGAAGCCAAGAGCGGCGACAAGAAGATCGGCACCACCGGCCGGGGCA
TTGGTCCCACCTACGAGGACAAGATCGGCCGTCGGGGGATCCGCCTCATGGATTTGCTCGACCCCGAAATATTCGCCCGC
AAGCTCCGCGAGAACCTGGAGGAGAAAAACTTCATCCTCGAAAAGCTCCTGGGCGAAAAACCTCTCTCCTTCGATGAGAT
CCACAAAACCTACAGCGGCTACGCCGATATCCTCCGGAAGTATGTTGCCAACACTTCCCTGATTCTCTGCAAGGACATCG
CGGCGGGGAAGAAGCTCCTCTTCGAGGGTGCCCAGGGAACCCTTCTCGACGTGGACCACGGCACCTATCCGTTCGTCACC
TCTTCGTCCACCTGCGCGGGAGGCGCCTGTACGGGAACAGGGGTTGGCCCCCGGGATATCCACGAAATTATCGGCATCTC
CAAGGCGTACGTGACGCGTGTCGGCAGCGGCCCCTTCCCCACCGAGCTGCTGGACGCCGACGGCGAGCAACTGCGCCAGA
CCGGCGGCGAGTTTGGCGCCACCACCGGCAGGCCCCGGCGCTGCGGCTGGTTCGATGCCATGGTGGTCCGCTTCGCCGTG
CGGGTCAACGGCCTTACCGGCGTGGCGCTCACCAAGCTGGACGTCCTGAACGATTTCGATACCATCAAGATCTGCACCGG
CTACACCTACGAGGGGAAACCGGTTGAGGACCTGCCGGCCAACCTGGCGGTATTCGAGAAGTGCCAGCCGGTCTACGAGG
AAATGCCCGGCTGGAAGAGCGACATCCGCGGCGTTCGCCGGTTCGAGGATCTCCCGGAGAAGGCCCGGCTCTATGTCCAT
CGGCTCCAGGAGCTGATCAGCTGCCCCATTGTCCTTGTTTCGATCGGTCCAGGCCGCGACGAAACCATAACGATCAGAAA
CCCGTTTGCGTAA

Upstream 100 bases:

>100_bases
GTTGTACGGGTGGCAGACAAACGGGGGACGGCCGTCAAGAAGGCCGATCTCATGCGGAACGATTTTTCGCTCAATACCCT
TCCCTGACAAGGAGAAGAAC

Downstream 100 bases:

>100_bases
AAAATTGAGCGTTTGGAAATTTTTTCTTGACGCATCGCAATAGGTTTGATAAAAGATGTTTTCTCTTTGAGAGCCGCTAG
CTCAGCTGGTAGAGCACCTG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVNGEKTVLHLIPSGILHKGKRCIIGNGVVLDPEVFI
MEINRLKEKGRLQDDSALLVSESVHIIMPYHKQIDLAREAKSGDKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDPEIFAR
KLRENLEEKNFILEKLLGEKPLSFDEIHKTYSGYADILRKYVANTSLILCKDIAAGKKLLFEGAQGTLLDVDHGTYPFVT
SSSTCAGGACTGTGVGPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEQLRQTGGEFGATTGRPRRCGWFDAMVVRFAV
RVNGLTGVALTKLDVLNDFDTIKICTGYTYEGKPVEDLPANLAVFEKCQPVYEEMPGWKSDIRGVRRFEDLPEKARLYVH
RLQELISCPIVLVSIGPGRDETITIRNPFA

Sequences:

>Translated_430_residues
MANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVNGEKTVLHLIPSGILHKGKRCIIGNGVVLDPEVFI
MEINRLKEKGRLQDDSALLVSESVHIIMPYHKQIDLAREAKSGDKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDPEIFAR
KLRENLEEKNFILEKLLGEKPLSFDEIHKTYSGYADILRKYVANTSLILCKDIAAGKKLLFEGAQGTLLDVDHGTYPFVT
SSSTCAGGACTGTGVGPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEQLRQTGGEFGATTGRPRRCGWFDAMVVRFAV
RVNGLTGVALTKLDVLNDFDTIKICTGYTYEGKPVEDLPANLAVFEKCQPVYEEMPGWKSDIRGVRRFEDLPEKARLYVH
RLQELISCPIVLVSIGPGRDETITIRNPFA
>Mature_429_residues
ANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVNGEKTVLHLIPSGILHKGKRCIIGNGVVLDPEVFIM
EINRLKEKGRLQDDSALLVSESVHIIMPYHKQIDLAREAKSGDKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDPEIFARK
LRENLEEKNFILEKLLGEKPLSFDEIHKTYSGYADILRKYVANTSLILCKDIAAGKKLLFEGAQGTLLDVDHGTYPFVTS
SSTCAGGACTGTGVGPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEQLRQTGGEFGATTGRPRRCGWFDAMVVRFAVR
VNGLTGVALTKLDVLNDFDTIKICTGYTYEGKPVEDLPANLAVFEKCQPVYEEMPGWKSDIRGVRRFEDLPEKARLYVHR
LQELISCPIVLVSIGPGRDETITIRNPFA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=41.6861826697892, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=40.8878504672897, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=39.6984924623116, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=52.1028037383178, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=44.6261682242991, Blast_Score=350, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=44.6261682242991, Blast_Score=350, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=41.5704387990762, Blast_Score=345, Evalue=6e-96,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=41.0377358490566, Blast_Score=331, Evalue=7e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_GEOMG (Q39QK1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386198.1
- ProteinModelPortal:   Q39QK1
- SMR:   Q39QK1
- STRING:   Q39QK1
- GeneID:   3741279
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3260
- NMPDR:   fig|269799.3.peg.2495
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q39QK1
- ProtClustDB:   PRK01117
- BioCyc:   GMET269799:GMET_3260-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47281; Mature: 47150

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVNGEKTVLHLIPSGIL
CCCEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEEECCCCEEEEECCHHHH
HKGKRCIIGNGVVLDPEVFIMEINRLKEKGRLQDDSALLVSESVHIIMPYHKQIDLAREA
HCCCEEEEECCEEECCCEEEHHHHHHHHHCCCCCCCEEEEECCEEEEECCHHHHHHHHHH
KSGDKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDPEIFARKLRENLEEKNFILEKLLGEK
CCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PLSFDEIHKTYSGYADILRKYVANTSLILCKDIAAGKKLLFEGAQGTLLDVDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCEEEECCCCCEEECCCCCCCEEE
SSSTCAGGACTGTGVGPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEQLRQTGGEFGA
CCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCHHHHCCCCHHHHHCCCCCCC
TTGRPRRCGWFDAMVVRFAVRVNGLTGVALTKLDVLNDFDTIKICTGYTYEGKPVEDLPA
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCC
NLAVFEKCQPVYEEMPGWKSDIRGVRRFEDLPEKARLYVHRLQELISCPIVLVSIGPGRD
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
ETITIRNPFA
CEEEEECCCC
>Mature Secondary Structure 
ANVVVVGAQWGDEGKGKVVDIYTEHADDVVRYQGGNNAGHTLVVNGEKTVLHLIPSGIL
CCEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCEEEECCCCEEEEECCHHHH
HKGKRCIIGNGVVLDPEVFIMEINRLKEKGRLQDDSALLVSESVHIIMPYHKQIDLAREA
HCCCEEEEECCEEECCCEEEHHHHHHHHHCCCCCCCEEEEECCEEEEECCHHHHHHHHHH
KSGDKKIGTTGRGIGPTYEDKIGRRGIRLMDLLDPEIFARKLRENLEEKNFILEKLLGEK
CCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PLSFDEIHKTYSGYADILRKYVANTSLILCKDIAAGKKLLFEGAQGTLLDVDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCEEEECCCCCEEECCCCCCCEEE
SSSTCAGGACTGTGVGPRDIHEIIGISKAYVTRVGSGPFPTELLDADGEQLRQTGGEFGA
CCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCHHHHCCCCHHHHHCCCCCCC
TTGRPRRCGWFDAMVVRFAVRVNGLTGVALTKLDVLNDFDTIKICTGYTYEGKPVEDLPA
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCC
NLAVFEKCQPVYEEMPGWKSDIRGVRRFEDLPEKARLYVHRLQELISCPIVLVSIGPGRD
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
ETITIRNPFA
CEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA