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The map label for this gene is hisZ

Identifier: 78224450

GI number: 78224450

Start: 3667602

End: 3668906

Strand: Direct

Name: hisZ

Synonym: Gmet_3259

Alternate gene names: 78224450

Gene position: 3667602-3668906 (Clockwise)

Preceding gene: 78224449

Following gene: 78224451

Centisome position: 91.75

GC content: 63.37

Gene sequence:

>1305_bases
TTGACAAATCCCGCTCCCATAGAAGCGCCCCTTCCCAAGGGGGTAACCGACTTTCTTCCCGAAAAGGCCGACAAGATCGG
CTACATCGAAGGGAAAATCCGCAGGGTGTTCGAACTCTGGGGCTTCCGGCGGATCATCACCCCGCTCCTGGAGTTCCAGG
ACGTCATGGCCGCGGGCCTCGGCGAGGATCTCAAGGAGCGGACCTTCCGCTTCGACGACCGCCAAACCGGTAAGCTCCTG
GCCATTCCCTCGGACATCACCCCCCAGGTGGCCCGTATCGTCGCAACCCGCATGCGGGGCTGCCCCCTTCCCCACCGACT
TTACTATATCGGCCGGGTGCTCAGGCACGTGGAACTCCAGTCGGGTCGAAGCCGCGAGACGTTTCAGGCTGGGGTCGAGC
TGATCGGGCTCGATTCCCCCGAGGCTGATGCCGAGATGGTTGCCATGGCGGTGGAAATCCTGAAGGGGCTCGGGTTCGAA
GAATTCAAGGTGGATCTGGGGCATACGGGGTTCATCCGCGGGGTCATGGCGGCTTCGGGGCTCGGCGGCGATGCGCGCCG
GCGGCTCCAGGAGGCAGTGGGGAAGAAGGACTCGTCGGCGGTCAGGGCAATCCTCGAAACCGAGCCGGTCGCGGACCGGA
TCAAGGAAGAGCTCGCCGCGCTGCCGCGCCTATTTGGCGGGCGCGAGGTGCTGGCCGAAGCGGCGCGGGTTGCTACTTCC
GATTCATCCCGCCGGGCACTGGACAATATCGCGCAGGTGCTGGACATTCTGGATATCCACGGCGTTTCCGACCACCTGAC
CCTCGACCTGGGGGAGATCCGGGGGCTCGATTACCACAGCGGCCTCACGTTCGAGGGGTTCGTGACCGGAATCGGCGAGG
CGGTCTGCAGCGGCGGGCGCTACGACAACCTGACACAGCGCTACGGATATCCAGCCCCGGCCACCGGCTTCGCCTTCAAT
ATCCTGGCCCTCCTGAACGCACTGGAAAAGCGGCCCGATGTGGAGGCGAGCAAGACCCGCGATCTCCTCATCTTCAACCT
CAAGGACGACCGCCGGGAGGCCCTCGAAATCGCCCAGCACCTGCGGGCACTGGGTTACTCCACCGCCCGCGACATCATCC
ACAGGGACTTCAACGACTCCCTCGATTATGCCCGGCGGATGAATATCCTCAGAATGATGGTAATCGGCGGCGACTACTGC
GCAGCCGACGAAGCATACGTTGTACGGGTGGCAGACAAACGGGGGACGGCCGTCAAGAAGGCCGATCTCATGCGGAACGA
TTTTTCGCTCAATACCCTTCCCTGA

Upstream 100 bases:

>100_bases
CGAGGGGATATATTTCGTTGACATCGAACCCGTATGCTGCTACGATTTTTCCCGTTTTGCTTACGGTTTAGCACTTTTTT
TCACTTCACAGGACACGCGT

Downstream 100 bases:

>100_bases
CAAGGAGAAGAACATGGCAAACGTGGTAGTGGTTGGCGCCCAGTGGGGCGATGAAGGAAAAGGCAAGGTTGTCGATATCT
ACACCGAGCATGCAGACGAC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTNPAPIEAPLPKGVTDFLPEKADKIGYIEGKIRRVFELWGFRRIITPLLEFQDVMAAGLGEDLKERTFRFDDRQTGKLL
AIPSDITPQVARIVATRMRGCPLPHRLYYIGRVLRHVELQSGRSRETFQAGVELIGLDSPEADAEMVAMAVEILKGLGFE
EFKVDLGHTGFIRGVMAASGLGGDARRRLQEAVGKKDSSAVRAILETEPVADRIKEELAALPRLFGGREVLAEAARVATS
DSSRRALDNIAQVLDILDIHGVSDHLTLDLGEIRGLDYHSGLTFEGFVTGIGEAVCSGGRYDNLTQRYGYPAPATGFAFN
ILALLNALEKRPDVEASKTRDLLIFNLKDDRREALEIAQHLRALGYSTARDIIHRDFNDSLDYARRMNILRMMVIGGDYC
AADEAYVVRVADKRGTAVKKADLMRNDFSLNTLP

Sequences:

>Translated_434_residues
MTNPAPIEAPLPKGVTDFLPEKADKIGYIEGKIRRVFELWGFRRIITPLLEFQDVMAAGLGEDLKERTFRFDDRQTGKLL
AIPSDITPQVARIVATRMRGCPLPHRLYYIGRVLRHVELQSGRSRETFQAGVELIGLDSPEADAEMVAMAVEILKGLGFE
EFKVDLGHTGFIRGVMAASGLGGDARRRLQEAVGKKDSSAVRAILETEPVADRIKEELAALPRLFGGREVLAEAARVATS
DSSRRALDNIAQVLDILDIHGVSDHLTLDLGEIRGLDYHSGLTFEGFVTGIGEAVCSGGRYDNLTQRYGYPAPATGFAFN
ILALLNALEKRPDVEASKTRDLLIFNLKDDRREALEIAQHLRALGYSTARDIIHRDFNDSLDYARRMNILRMMVIGGDYC
AADEAYVVRVADKRGTAVKKADLMRNDFSLNTLP
>Mature_433_residues
TNPAPIEAPLPKGVTDFLPEKADKIGYIEGKIRRVFELWGFRRIITPLLEFQDVMAAGLGEDLKERTFRFDDRQTGKLLA
IPSDITPQVARIVATRMRGCPLPHRLYYIGRVLRHVELQSGRSRETFQAGVELIGLDSPEADAEMVAMAVEILKGLGFEE
FKVDLGHTGFIRGVMAASGLGGDARRRLQEAVGKKDSSAVRAILETEPVADRIKEELAALPRLFGGREVLAEAARVATSD
SSRRALDNIAQVLDILDIHGVSDHLTLDLGEIRGLDYHSGLTFEGFVTGIGEAVCSGGRYDNLTQRYGYPAPATGFAFNI
LALLNALEKRPDVEASKTRDLLIFNLKDDRREALEIAQHLRALGYSTARDIIHRDFNDSLDYARRMNILRMMVIGGDYCA
ADEAYVVRVADKRGTAVKKADLMRNDFSLNTLP

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI15029520, Length=353, Percent_Identity=24.3626062322946, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI6996014, Length=354, Percent_Identity=25.7062146892655, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1788861, Length=365, Percent_Identity=27.9452054794521, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71993693, Length=400, Percent_Identity=25.25, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71993686, Length=400, Percent_Identity=25.25, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325290, Length=363, Percent_Identity=26.1707988980716, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24643061, Length=369, Percent_Identity=28.4552845528455, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24643059, Length=388, Percent_Identity=27.0618556701031, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_GEOMG (Q39QK2)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386197.1
- ProteinModelPortal:   Q39QK2
- SMR:   Q39QK2
- STRING:   Q39QK2
- GeneID:   3741278
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3259
- NMPDR:   fig|269799.3.peg.2494
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   YREHVKS
- PhylomeDB:   Q39QK2
- ProtClustDB:   PRK12292
- BioCyc:   GMET269799:GMET_3259-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004517
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 48055; Mature: 47924

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPAPIEAPLPKGVTDFLPEKADKIGYIEGKIRRVFELWGFRRIITPLLEFQDVMAAGL
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEDLKERTFRFDDRQTGKLLAIPSDITPQVARIVATRMRGCPLPHRLYYIGRVLRHVELQ
CHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SGRSRETFQAGVELIGLDSPEADAEMVAMAVEILKGLGFEEFKVDLGHTGFIRGVMAASG
CCCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCHHHHHHHHHHCC
LGGDARRRLQEAVGKKDSSAVRAILETEPVADRIKEELAALPRLFGGREVLAEAARVATS
CCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DSSRRALDNIAQVLDILDIHGVSDHLTLDLGEIRGLDYHSGLTFEGFVTGIGEAVCSGGR
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
YDNLTQRYGYPAPATGFAFNILALLNALEKRPDVEASKTRDLLIFNLKDDRREALEIAQH
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
LRALGYSTARDIIHRDFNDSLDYARRMNILRMMVIGGDYCAADEAYVVRVADKRGTAVKK
HHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHH
ADLMRNDFSLNTLP
HHHHHCCCCCCCCC
>Mature Secondary Structure 
TNPAPIEAPLPKGVTDFLPEKADKIGYIEGKIRRVFELWGFRRIITPLLEFQDVMAAGL
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEDLKERTFRFDDRQTGKLLAIPSDITPQVARIVATRMRGCPLPHRLYYIGRVLRHVELQ
CHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SGRSRETFQAGVELIGLDSPEADAEMVAMAVEILKGLGFEEFKVDLGHTGFIRGVMAASG
CCCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCHHEEEECCCHHHHHHHHHHCC
LGGDARRRLQEAVGKKDSSAVRAILETEPVADRIKEELAALPRLFGGREVLAEAARVATS
CCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DSSRRALDNIAQVLDILDIHGVSDHLTLDLGEIRGLDYHSGLTFEGFVTGIGEAVCSGGR
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
YDNLTQRYGYPAPATGFAFNILALLNALEKRPDVEASKTRDLLIFNLKDDRREALEIAQH
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
LRALGYSTARDIIHRDFNDSLDYARRMNILRMMVIGGDYCAADEAYVVRVADKRGTAVKK
HHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHH
ADLMRNDFSLNTLP
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA