Click here to switch to the map view.

The map label for this gene is nirB [C]

Identifier: 78224413

GI number: 78224413

Start: 3622483

End: 3623148

Strand: Direct

Name: nirB [C]

Synonym: Gmet_3222

Alternate gene names: 78224413

Gene position: 3622483-3623148 (Clockwise)

Preceding gene: 78224412

Following gene: 78224415

Centisome position: 90.62

GC content: 63.21

Gene sequence:

>666_bases
ATGAAAAAGGACATCCTCGAAAAGGGGGCAATCGTCCAGCGGGACCGGGAAACCTACGCCATCGCCCCCCACATCCCCGG
CGGCATCACCGATACCGCCACCCTCCGCAAGATCTGCGACGTGGCCGACCGCTACGGCGTCAAGGAACTGAAACTCACGT
CGGCCCAGCGCATCGCCCTCATGGGGGTGAAGGAAGAGGACCTGGACACCATCTGGAGCGACCTGGACCAGCAACCAGGG
GCGGCCATCGGCCTCTGCGTCAGGAGCGTCAAGATCTGCCCCGGCACCACCTGGTGCAAGCGGGGGGTGCAGGATTCAGT
GGCCCTCGGCCTCAAGATCGACACCATCTACCACGCCATGAAGCTTCCCAACAAGATGAAGATGGGGATTTCGGGGTGCA
TGGTCAACTGCGCCGAAACGCTGCTGAAGGACATCGGGGTGGTGGGGACGCCGAAAGGGTGGCGGATCTACGTGGGGGGA
AACGCCGGCGCCCGGCCCCGCATCGGGGAGGTATTCACCGATACCGCTCCTGACGATGACGAGGTTCTGGCTGTTGTTGC
ACGGATCGTCGACTACTACAAAGGCTCCGGAAGCGAGCAGAGGCTGGGGCGGATCGTGGAGCAGATGGGGATCGAGACGT
TCCGGCAGGCAGTTCTCGGCACCTGA

Upstream 100 bases:

>100_bases
CATGGCAAAGGCTGAGTAGCCTCTGGCATCGCCAATCCCCTTCCGTTCCCCCCTGAACTATGCTAATGAAAGGGTTATTT
TCCAATCCAGGAGTCAGTCA

Downstream 100 bases:

>100_bases
CGGAACACCCCTCAGAACAGCTCCAGTTGCTCCCCCTTCTTCCCCGTGCGCACGGTATCCTGAACGGCGCGGCTGACAAG
CCGCGCCGGAATCTCCTCCA

Product: nitrite and sulphite reductase 4Fe-4S region

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKKDILEKGAIVQRDRETYAIAPHIPGGITDTATLRKICDVADRYGVKELKLTSAQRIALMGVKEEDLDTIWSDLDQQPG
AAIGLCVRSVKICPGTTWCKRGVQDSVALGLKIDTIYHAMKLPNKMKMGISGCMVNCAETLLKDIGVVGTPKGWRIYVGG
NAGARPRIGEVFTDTAPDDDEVLAVVARIVDYYKGSGSEQRLGRIVEQMGIETFRQAVLGT

Sequences:

>Translated_221_residues
MKKDILEKGAIVQRDRETYAIAPHIPGGITDTATLRKICDVADRYGVKELKLTSAQRIALMGVKEEDLDTIWSDLDQQPG
AAIGLCVRSVKICPGTTWCKRGVQDSVALGLKIDTIYHAMKLPNKMKMGISGCMVNCAETLLKDIGVVGTPKGWRIYVGG
NAGARPRIGEVFTDTAPDDDEVLAVVARIVDYYKGSGSEQRLGRIVEQMGIETFRQAVLGT
>Mature_221_residues
MKKDILEKGAIVQRDRETYAIAPHIPGGITDTATLRKICDVADRYGVKELKLTSAQRIALMGVKEEDLDTIWSDLDQQPG
AAIGLCVRSVKICPGTTWCKRGVQDSVALGLKIDTIYHAMKLPNKMKMGISGCMVNCAETLLKDIGVVGTPKGWRIYVGG
NAGARPRIGEVFTDTAPDDDEVLAVVARIVDYYKGSGSEQRLGRIVEQMGIETFRQAVLGT

Specific function: This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789765, Length=187, Percent_Identity=35.2941176470588, Blast_Score=129, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR017220 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 24039; Mature: 24039

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDILEKGAIVQRDRETYAIAPHIPGGITDTATLRKICDVADRYGVKELKLTSAQRIAL
CCHHHHHCCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHEE
MGVKEEDLDTIWSDLDQQPGAAIGLCVRSVKICPGTTWCKRGVQDSVALGLKIDTIYHAM
ECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHCCHHHHHHHHH
KLPNKMKMGISGCMVNCAETLLKDIGVVGTPKGWRIYVGGNAGARPRIGEVFTDTAPDDD
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHCCCCCCHH
EVLAVVARIVDYYKGSGSEQRLGRIVEQMGIETFRQAVLGT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKDILEKGAIVQRDRETYAIAPHIPGGITDTATLRKICDVADRYGVKELKLTSAQRIAL
CCHHHHHCCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHEE
MGVKEEDLDTIWSDLDQQPGAAIGLCVRSVKICPGTTWCKRGVQDSVALGLKIDTIYHAM
ECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHCCHHHHHHHHH
KLPNKMKMGISGCMVNCAETLLKDIGVVGTPKGWRIYVGGNAGARPRIGEVFTDTAPDDD
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHCCCCCCHH
EVLAVVARIVDYYKGSGSEQRLGRIVEQMGIETFRQAVLGT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1911781 [H]