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The map label for this gene is proB

Identifier: 78224389

GI number: 78224389

Start: 3598666

End: 3599787

Strand: Direct

Name: proB

Synonym: Gmet_3198

Alternate gene names: 78224389

Gene position: 3598666-3599787 (Clockwise)

Preceding gene: 78224388

Following gene: 78224390

Centisome position: 90.02

GC content: 66.31

Gene sequence:

>1122_bases
ATGCGCACAGCCATCCTCAAAAAGGTCAAACGGATCGTCATCAAGATCGGGAGCCGTGTCCTCACCGGCGACGAGAACGG
CCTCAACCACGCCTTCATCGCCCGGCTTGCCGCCGAGACCGCATCGTTGCGACAGCAGGGGCGCCAGGTCATCGTCGTTT
CATCGGGTGCCGTGGCTGCCGGCAGGCGCGAACTCGGCATCGAGGGACGGCCCAAGAGCATTCCCCAGAAGCAGGCCGCT
GCCGCCATCGGCCAGTCACGCCTCATGCGGGCCTACGAAGAGGACTTCGGCCGCTTCGGCCACAAGGTGGCACAGATACT
CCTCACCCGGGACGATCTCGCCAACCGCCGCCGTTTCCTGAACGCCCGGGCAACCCTTGACACGCTCCTGGACTTCGGCG
TCATTCCCGTCATCAACGAGAACGACACCGTGGTGGTGGACGAGATCAAGTTCGGCGACAACGACAACCTGTCGGCCCTG
GTGACCAACCTGGCCGAGGCCCACCTCCTGGTGATCCTGACCGACATCGACGGCTTCTACGACTCCGACCCCCGGGCGAA
CCCCGATGCCAGGCTGATCCACCTGGTGAAGAGCATCACCCGCGAAACGGAGCGGGCCGCCGGCGACAGCGGCTCCAGCG
TGGGCACCGGCGGCATGGTGACGAAGCTGGCCGCAGCCAAGAAGGCGGGGCAGTTCGGCGTCCCGACCCTCATGCTCAAC
GGCACGGTGCCGGGCATTCTCACCAAGGCCTTTGCCGGCCGGGAGGTGGGAACCCTCTTCCTTCCCGCCCGGGAGAGCCT
CAACCGCCGCAAGCACTGGATCGCCCACACCCTGCGCCCCAGCGGCCGAGTCATCGTGGACGACGGAGCCCGGACGGTCC
TCTCCCGTCAGGGGAAAAGTCTCCTCCCCTCGGGGGTAATCCGAGTGGAGGGAGAGTTCGACCGAGGGGCCTGTGTCCGG
GTTTGCGGGGCCGACGGCGCCGAAATCGCCCGGGGGGTGGTGGACTACTCCCACGCCGAAATCGAGCGGATCCTCGGCCA
CCGGAGCGGCGAGATCGAGGCGATCCTCGGCTACAAGTACGGAGACGAGATCATCCACCGGGACAACCTGGTGGTGCTAT
AA

Upstream 100 bases:

>100_bases
CCCATCTCCGCCGCCACCGGCGAGGGAATCCCGACCCTCCTCGACGAAATCGCCCGGCATCTCTGGGGCCAGGCCGAGGA
AGAGTGGTAGCGTGGTACCA

Downstream 100 bases:

>100_bases
AGAAAGAATTCAGGAGTCAGAATTCAGAATCCAGTAAGTTTTTCTCCTGAATTCTGACTCCTGTCTCCTGACTACTCATA
ACGGAGGTACCTCCCGTGAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MRTAILKKVKRIVIKIGSRVLTGDENGLNHAFIARLAAETASLRQQGRQVIVVSSGAVAAGRRELGIEGRPKSIPQKQAA
AAIGQSRLMRAYEEDFGRFGHKVAQILLTRDDLANRRRFLNARATLDTLLDFGVIPVINENDTVVVDEIKFGDNDNLSAL
VTNLAEAHLLVILTDIDGFYDSDPRANPDARLIHLVKSITRETERAAGDSGSSVGTGGMVTKLAAAKKAGQFGVPTLMLN
GTVPGILTKAFAGREVGTLFLPARESLNRRKHWIAHTLRPSGRVIVDDGARTVLSRQGKSLLPSGVIRVEGEFDRGACVR
VCGADGAEIARGVVDYSHAEIERILGHRSGEIEAILGYKYGDEIIHRDNLVVL

Sequences:

>Translated_373_residues
MRTAILKKVKRIVIKIGSRVLTGDENGLNHAFIARLAAETASLRQQGRQVIVVSSGAVAAGRRELGIEGRPKSIPQKQAA
AAIGQSRLMRAYEEDFGRFGHKVAQILLTRDDLANRRRFLNARATLDTLLDFGVIPVINENDTVVVDEIKFGDNDNLSAL
VTNLAEAHLLVILTDIDGFYDSDPRANPDARLIHLVKSITRETERAAGDSGSSVGTGGMVTKLAAAKKAGQFGVPTLMLN
GTVPGILTKAFAGREVGTLFLPARESLNRRKHWIAHTLRPSGRVIVDDGARTVLSRQGKSLLPSGVIRVEGEFDRGACVR
VCGADGAEIARGVVDYSHAEIERILGHRSGEIEAILGYKYGDEIIHRDNLVVL
>Mature_373_residues
MRTAILKKVKRIVIKIGSRVLTGDENGLNHAFIARLAAETASLRQQGRQVIVVSSGAVAAGRRELGIEGRPKSIPQKQAA
AAIGQSRLMRAYEEDFGRFGHKVAQILLTRDDLANRRRFLNARATLDTLLDFGVIPVINENDTVVVDEIKFGDNDNLSAL
VTNLAEAHLLVILTDIDGFYDSDPRANPDARLIHLVKSITRETERAAGDSGSSVGTGGMVTKLAAAKKAGQFGVPTLMLN
GTVPGILTKAFAGREVGTLFLPARESLNRRKHWIAHTLRPSGRVIVDDGARTVLSRQGKSLLPSGVIRVEGEFDRGACVR
VCGADGAEIARGVVDYSHAEIERILGHRSGEIEAILGYKYGDEIIHRDNLVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=295, Percent_Identity=35.2542372881356, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI62912457, Length=293, Percent_Identity=35.1535836177474, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=46.7213114754098, Blast_Score=319, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17569969, Length=294, Percent_Identity=35.7142857142857, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17569967, Length=294, Percent_Identity=35.7142857142857, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320506, Length=425, Percent_Identity=37.1764705882353, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321822, Length=419, Percent_Identity=34.8448687350835, Blast_Score=222, Evalue=7e-59,
Organism=Drosophila melanogaster, GI21357643, Length=295, Percent_Identity=34.9152542372881, Blast_Score=151, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_GEOMG (Q39QR3)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386136.1
- ProteinModelPortal:   Q39QR3
- STRING:   Q39QR3
- GeneID:   3741108
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3198
- NMPDR:   fig|269799.3.peg.2432
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q39QR3
- ProtClustDB:   PRK05429
- BioCyc:   GMET269799:GMET_3198-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40359; Mature: 40359

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTAILKKVKRIVIKIGSRVLTGDENGLNHAFIARLAAETASLRQQGRQVIVVSSGAVAA
CCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GRRELGIEGRPKSIPQKQAAAAIGQSRLMRAYEEDFGRFGHKVAQILLTRDDLANRRRFL
CCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NARATLDTLLDFGVIPVINENDTVVVDEIKFGDNDNLSALVTNLAEAHLLVILTDIDGFY
HHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHCCEEEEEEEECCCCC
DSDPRANPDARLIHLVKSITRETERAAGDSGSSVGTGGMVTKLAAAKKAGQFGVPTLMLN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
GTVPGILTKAFAGREVGTLFLPARESLNRRKHWIAHTLRPSGRVIVDDGARTVLSRQGKS
CCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCC
LLPSGVIRVEGEFDRGACVRVCGADGAEIARGVVDYSHAEIERILGHRSGEIEAILGYKY
CCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
GDEIIHRDNLVVL
CCHHEECCCEEEC
>Mature Secondary Structure
MRTAILKKVKRIVIKIGSRVLTGDENGLNHAFIARLAAETASLRQQGRQVIVVSSGAVAA
CCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GRRELGIEGRPKSIPQKQAAAAIGQSRLMRAYEEDFGRFGHKVAQILLTRDDLANRRRFL
CCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NARATLDTLLDFGVIPVINENDTVVVDEIKFGDNDNLSALVTNLAEAHLLVILTDIDGFY
HHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHCCEEEEEEEECCCCC
DSDPRANPDARLIHLVKSITRETERAAGDSGSSVGTGGMVTKLAAAKKAGQFGVPTLMLN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
GTVPGILTKAFAGREVGTLFLPARESLNRRKHWIAHTLRPSGRVIVDDGARTVLSRQGKS
CCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCC
LLPSGVIRVEGEFDRGACVRVCGADGAEIARGVVDYSHAEIERILGHRSGEIEAILGYKY
CCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
GDEIIHRDNLVVL
CCHHEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA