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The map label for this gene is purT

Identifier: 78224384

GI number: 78224384

Start: 3595319

End: 3596497

Strand: Direct

Name: purT

Synonym: Gmet_3193

Alternate gene names: 78224384

Gene position: 3595319-3596497 (Clockwise)

Preceding gene: 78224383

Following gene: 78224385

Centisome position: 89.94

GC content: 64.89

Gene sequence:

>1179_bases
ATGATTGGAACCCCTCTCAAGAAAAGCGCCACGCGCGTCATGCTCCTCGGCTCCGGCGAGCTCGGGAAAGAGGTTGTGCT
CGAAGCCCAGCGGCTCGGCGTCGAGGTAATCGCCGTCGACCGCTACGCCGATGCGCCCGCCATGCAGGTGGCCCACCGCG
CCCATGTCGTCAACATGCTCGACCGGGTCGAACTCGGCCGGATCGTGGCCCGGGAACGGCCCCACCTTATCGTCCCGGAA
ATCGAGGCGATCGACACCCCCTACCTCCTGGAACTGGAGCAGGAGGGGTACACCGTGATCCCCACGGCCCGGGCCGCCAA
CCTGACCATGAACCGGGAAGGCATCCGGCGCCTGGCGGCGGAAGAGCTGGGCCTGCCAACGGCTGCCTATCGCTTTGCCG
CGAGTATCGAATCGTTCCGGGCGGCGGTGAAGGATATCGGCCTTCCCTGCGTGGTGAAACCAATCATGAGTTCCTCCGGC
AAGGGACAAAGCGTTGTAAAGAGTATGGAAGAAATCGACGGCGCCTGGACCTACGCCATGGAAGGGGGCCGGGGGGCCTC
CGATACCGTAATCGTTGAGGAATTCATTCCCTTTGACTACGAGATCACGCTCCTGACCGTGCGCCACGCCGGGGGCACCA
CCTTCTGCCCTCCCATTGGCCACGTGCAGATCAAGGGGGACTACCACGAGTCATGGCAGCCCATGGCCATGACGCCGGCC
GCCCTGGCGGAATCCCAGCGCCAGGCCAAAGCAGTGACCGACGCTCTCGGCGGCTCCGGGATCTTCGGGGTGGAACTCTT
CATCAAGGGAGATCGGGTCTGGTTCAGCGAGGTTTCACCCCGCCCCCACGACACCGGCATGGTCACGATGATCTCCCAGA
ACCTCTCCGAGTTCGAGCTTCACGTCCGGGCAATCCTTGGCCTTCCCGTTCCGGAGGTGGCAAACCTGGCCCCGGCGGCC
TCCCATGTCGTCCTGGCCTCGGAAGCAGCCGAGGAGGTCACCTTCTCCGGCCTGGACGCCGCCCTCTCTGTCCCGGAAAC
GAAGCTGCGCCTCTTTGGAAAACCCGACACCCGGCCGGGACGACGGATGGGGGTCGCACTCTCCTTCGGCGCCGACACCG
ACGAGGCGAGAAACCGGGCCGAGCAGGCAGCCCATGCAGTGAAGATCGTCACCCTCTGA

Upstream 100 bases:

>100_bases
GGAGAAGAAATTCAAGAAGCGGATCACCGTCCGGGCCAAGCCGGGCTTCCACCAGGAGCAGTTCGAGATCGCCATCAGCT
AGGAACAAAGGAATTCTTCC

Downstream 100 bases:

>100_bases
CCATCATTCAAAAGGAGGTTTGTCATGGATTTCGCGGCCAAAGTTTCCGATGTACCCGACTGGGGAAAGAAAATTGTCAC
CGTCAAGGGCCAGGAGATCC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MIGTPLKKSATRVMLLGSGELGKEVVLEAQRLGVEVIAVDRYADAPAMQVAHRAHVVNMLDRVELGRIVARERPHLIVPE
IEAIDTPYLLELEQEGYTVIPTARAANLTMNREGIRRLAAEELGLPTAAYRFAASIESFRAAVKDIGLPCVVKPIMSSSG
KGQSVVKSMEEIDGAWTYAMEGGRGASDTVIVEEFIPFDYEITLLTVRHAGGTTFCPPIGHVQIKGDYHESWQPMAMTPA
ALAESQRQAKAVTDALGGSGIFGVELFIKGDRVWFSEVSPRPHDTGMVTMISQNLSEFELHVRAILGLPVPEVANLAPAA
SHVVLASEAAEEVTFSGLDAALSVPETKLRLFGKPDTRPGRRMGVALSFGADTDEARNRAEQAAHAVKIVTL

Sequences:

>Translated_392_residues
MIGTPLKKSATRVMLLGSGELGKEVVLEAQRLGVEVIAVDRYADAPAMQVAHRAHVVNMLDRVELGRIVARERPHLIVPE
IEAIDTPYLLELEQEGYTVIPTARAANLTMNREGIRRLAAEELGLPTAAYRFAASIESFRAAVKDIGLPCVVKPIMSSSG
KGQSVVKSMEEIDGAWTYAMEGGRGASDTVIVEEFIPFDYEITLLTVRHAGGTTFCPPIGHVQIKGDYHESWQPMAMTPA
ALAESQRQAKAVTDALGGSGIFGVELFIKGDRVWFSEVSPRPHDTGMVTMISQNLSEFELHVRAILGLPVPEVANLAPAA
SHVVLASEAAEEVTFSGLDAALSVPETKLRLFGKPDTRPGRRMGVALSFGADTDEARNRAEQAAHAVKIVTL
>Mature_392_residues
MIGTPLKKSATRVMLLGSGELGKEVVLEAQRLGVEVIAVDRYADAPAMQVAHRAHVVNMLDRVELGRIVARERPHLIVPE
IEAIDTPYLLELEQEGYTVIPTARAANLTMNREGIRRLAAEELGLPTAAYRFAASIESFRAAVKDIGLPCVVKPIMSSSG
KGQSVVKSMEEIDGAWTYAMEGGRGASDTVIVEEFIPFDYEITLLTVRHAGGTTFCPPIGHVQIKGDYHESWQPMAMTPA
ALAESQRQAKAVTDALGGSGIFGVELFIKGDRVWFSEVSPRPHDTGMVTMISQNLSEFELHVRAILGLPVPEVANLAPAA
SHVVLASEAAEEVTFSGLDAALSVPETKLRLFGKPDTRPGRRMGVALSFGADTDEARNRAEQAAHAVKIVTL

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=60.9254498714653, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1786733, Length=260, Percent_Identity=27.3076923076923, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=369, Percent_Identity=26.0162601626016, Blast_Score=116, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_GEOMG (Q39QR8)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386131.1
- ProteinModelPortal:   Q39QR8
- SMR:   Q39QR8
- STRING:   Q39QR8
- GeneID:   3741103
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3193
- NMPDR:   fig|269799.3.peg.2427
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   Q39QR8
- ProtClustDB:   PRK09288
- BioCyc:   GMET269799:GMET_3193-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42283; Mature: 42283

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 80-80 BINDING 112-112 BINDING 153-153 BINDING 201-201 BINDING 265-265 BINDING 277-277 BINDING 284-284 BINDING 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGTPLKKSATRVMLLGSGELGKEVVLEAQRLGVEVIAVDRYADAPAMQVAHRAHVVNML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
DRVELGRIVARERPHLIVPEIEAIDTPYLLELEQEGYTVIPTARAANLTMNREGIRRLAA
HHHHHHHHHHCCCCCEECCCHHHCCCCEEEEECCCCCEEEECCCCCCEEECHHHHHHHHH
EELGLPTAAYRFAASIESFRAAVKDIGLPCVVKPIMSSSGKGQSVVKSMEEIDGAWTYAM
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCHHHHHHHHHHCCEEEEEE
EGGRGASDTVIVEEFIPFDYEITLLTVRHAGGTTFCPPIGHVQIKGDYHESWQPMAMTPA
CCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCEECHH
ALAESQRQAKAVTDALGGSGIFGVELFIKGDRVWFSEVSPRPHDTGMVTMISQNLSEFEL
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHHHH
HVRAILGLPVPEVANLAPAASHVVLASEAAEEVTFSGLDAALSVPETKLRLFGKPDTRPG
HHHHHHCCCCHHHHHCCCCHHEEEEEHHHHHHHHHCCCCHHHCCCCHHEEEECCCCCCCC
RRMGVALSFGADTDEARNRAEQAAHAVKIVTL
CEEEEEEEECCCCHHHHHHHHHHHHEEEEEEC
>Mature Secondary Structure
MIGTPLKKSATRVMLLGSGELGKEVVLEAQRLGVEVIAVDRYADAPAMQVAHRAHVVNML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
DRVELGRIVARERPHLIVPEIEAIDTPYLLELEQEGYTVIPTARAANLTMNREGIRRLAA
HHHHHHHHHHCCCCCEECCCHHHCCCCEEEEECCCCCEEEECCCCCCEEECHHHHHHHHH
EELGLPTAAYRFAASIESFRAAVKDIGLPCVVKPIMSSSGKGQSVVKSMEEIDGAWTYAM
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCHHHHHHHHHHCCEEEEEE
EGGRGASDTVIVEEFIPFDYEITLLTVRHAGGTTFCPPIGHVQIKGDYHESWQPMAMTPA
CCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCEECHH
ALAESQRQAKAVTDALGGSGIFGVELFIKGDRVWFSEVSPRPHDTGMVTMISQNLSEFEL
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHHHH
HVRAILGLPVPEVANLAPAASHVVLASEAAEEVTFSGLDAALSVPETKLRLFGKPDTRPG
HHHHHHCCCCHHHHHCCCCHHEEEEEHHHHHHHHHCCCCHHHCCCCHHEEEECCCCCCCC
RRMGVALSFGADTDEARNRAEQAAHAVKIVTL
CEEEEEEEECCCCHHHHHHHHHHHHEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA