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The map label for this gene is mak [H]

Identifier: 78224001

GI number: 78224001

Start: 3167886

End: 3168779

Strand: Reverse

Name: mak [H]

Synonym: Gmet_2805

Alternate gene names: 78224001

Gene position: 3168779-3167886 (Counterclockwise)

Preceding gene: 78224002

Following gene: 78224000

Centisome position: 79.27

GC content: 68.9

Gene sequence:

>894_bases
GTGAATCCTTACCGCATCGGCATAGACCTGGGGGGGACCAAGATCGAGGCCCTGCTCCTCGGCCCCGAGGGGGAGGAGCA
GTCCCGGCGGCGGATTCCGTCCCCGGCCGTCATGGGGTACGAAGCGGTCGTCGCCGCCGTGGCTGGGCTGGTCGATGAGG
CGGCCGGCCGGATTCCGGAGGGTGCCCGCCGGACCATCGGCATCGGCATTCCCGGCTCCGTCGATCCGGTCACTGGGCTC
GTGCGCAATGCCAACTCCACCTGCCTCATCGGCAAGCCGCTCCAGGGGGACCTGGAGTCCCGCCTGGGGTGCTCCGTTGC
GCTTCGAAACGATGCCGACTGCTTCACCATGGCCGAGTGCAGGATGGGGGCCGGCCGCGGGTACGGCCTCGTCTTCGGCG
TCATCATGGGGACCGGCTGCGGCGGCGGTATCTGTATCGACGGCGTGGTGCGCGAGGGGCCCCACCGGATCGCCGGCGAG
TGGGGGCACATCTCGGTGGACCCTGCCGGCGCCCCTTGTTACTGCGGCAACCGGGGGTGCGTGGAGACGAAGATAAGCGG
CTCCGGCGTGGAGCGGGCCTTTTGTGCCGACCATGGTGAAGGGATTGTCATGGAGGAAATCGTCCGGTTGGCCCGTGAGG
GAGAGCCCCGCTGCACCGCCGCCTTCGACCGCTTCCTGGACGACTTCGGCCGCTGCCTCGGGGGGCTCATCTCCATCCTC
GACCCTGATGCCGTGGTCCTTGGCGGCGGGCTCTCCAATATCGACGAACTCTATACCATCGGCGTCGAGCGGGTGCACCG
GTACGCATTCCACGACGCGCTCCGGACGCCGATTCTCAGGAACCGGCTCGGCGATTCGGCCGGCGTCATCGGCGCCGCGT
GGATCGGGAGGTGA

Upstream 100 bases:

>100_bases
CTACACCGTGAAGCGGGCAGGGGGGTAGGATGAGGCGCTGGATCGTCCTTGCGGCTGCGACCCTGCGGCCGGCGCCGCTT
ACCGCTGGGGAGGAGGCGCC

Downstream 100 bases:

>100_bases
CACCATGAAACGTATCTGCGTCTTCTGCGGTTCCAGCCCCGGCAGGAACCCCGTCTATCGCGCCGCCGCCGAGGCGATGG
GCCGGCTTCTGGCCCGGGAG

Product: N-acetylglucosamine kinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNPYRIGIDLGGTKIEALLLGPEGEEQSRRRIPSPAVMGYEAVVAAVAGLVDEAAGRIPEGARRTIGIGIPGSVDPVTGL
VRNANSTCLIGKPLQGDLESRLGCSVALRNDADCFTMAECRMGAGRGYGLVFGVIMGTGCGGGICIDGVVREGPHRIAGE
WGHISVDPAGAPCYCGNRGCVETKISGSGVERAFCADHGEGIVMEEIVRLAREGEPRCTAAFDRFLDDFGRCLGGLISIL
DPDAVVLGGGLSNIDELYTIGVERVHRYAFHDALRTPILRNRLGDSAGVIGAAWIGR

Sequences:

>Translated_297_residues
MNPYRIGIDLGGTKIEALLLGPEGEEQSRRRIPSPAVMGYEAVVAAVAGLVDEAAGRIPEGARRTIGIGIPGSVDPVTGL
VRNANSTCLIGKPLQGDLESRLGCSVALRNDADCFTMAECRMGAGRGYGLVFGVIMGTGCGGGICIDGVVREGPHRIAGE
WGHISVDPAGAPCYCGNRGCVETKISGSGVERAFCADHGEGIVMEEIVRLAREGEPRCTAAFDRFLDDFGRCLGGLISIL
DPDAVVLGGGLSNIDELYTIGVERVHRYAFHDALRTPILRNRLGDSAGVIGAAWIGR
>Mature_297_residues
MNPYRIGIDLGGTKIEALLLGPEGEEQSRRRIPSPAVMGYEAVVAAVAGLVDEAAGRIPEGARRTIGIGIPGSVDPVTGL
VRNANSTCLIGKPLQGDLESRLGCSVALRNDADCFTMAECRMGAGRGYGLVFGVIMGTGCGGGICIDGVVREGPHRIAGE
WGHISVDPAGAPCYCGNRGCVETKISGSGVERAFCADHGEGIVMEEIVRLAREGEPRCTAAFDRFLDDFGRCLGGLISIL
DPDAVVLGGGLSNIDELYTIGVERVHRYAFHDALRTPILRNRLGDSAGVIGAAWIGR

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=268, Percent_Identity=27.2388059701493, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI298566315, Length=268, Percent_Identity=27.2388059701493, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI190014632, Length=272, Percent_Identity=29.0441176470588, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4885285, Length=268, Percent_Identity=27.2388059701493, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI87081733, Length=300, Percent_Identity=42.6666666666667, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1787363, Length=299, Percent_Identity=36.7892976588629, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1787878, Length=217, Percent_Identity=27.6497695852535, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786891, Length=310, Percent_Identity=23.2258064516129, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790522, Length=255, Percent_Identity=27.4509803921569, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082230, Length=249, Percent_Identity=31.3253012048193, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31106; Mature: 31106

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPYRIGIDLGGTKIEALLLGPEGEEQSRRRIPSPAVMGYEAVVAAVAGLVDEAAGRIPE
CCCEEEEEEECCCEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GARRTIGIGIPGSVDPVTGLVRNANSTCLIGKPLQGDLESRLGCSVALRNDADCFTMAEC
CCCCEEEECCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHCCCCEEEECCCCCCEEHHHH
RMGAGRGYGLVFGVIMGTGCGGGICIDGVVREGPHRIAGEWGHISVDPAGAPCYCGNRGC
HCCCCCCHHHHHHHHHHCCCCCCEEECCHHCCCCCHHCCCCCEEEECCCCCCEEECCCCC
VETKISGSGVERAFCADHGEGIVMEEIVRLAREGEPRCTAAFDRFLDDFGRCLGGLISIL
EEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DPDAVVLGGGLSNIDELYTIGVERVHRYAFHDALRTPILRNRLGDSAGVIGAAWIGR
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCEEHHCCCC
>Mature Secondary Structure
MNPYRIGIDLGGTKIEALLLGPEGEEQSRRRIPSPAVMGYEAVVAAVAGLVDEAAGRIPE
CCCEEEEEEECCCEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GARRTIGIGIPGSVDPVTGLVRNANSTCLIGKPLQGDLESRLGCSVALRNDADCFTMAEC
CCCCEEEECCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHCCCCEEEECCCCCCEEHHHH
RMGAGRGYGLVFGVIMGTGCGGGICIDGVVREGPHRIAGEWGHISVDPAGAPCYCGNRGC
HCCCCCCHHHHHHHHHHCCCCCCEEECCHHCCCCCHHCCCCCEEEECCCCCCEEECCCCC
VETKISGSGVERAFCADHGEGIVMEEIVRLAREGEPRCTAAFDRFLDDFGRCLGGLISIL
EEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
DPDAVVLGGGLSNIDELYTIGVERVHRYAFHDALRTPILRNRLGDSAGVIGAAWIGR
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]