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The map label for this gene is btuB [H]

Identifier: 78223931

GI number: 78223931

Start: 3091132

End: 3093084

Strand: Direct

Name: btuB [H]

Synonym: Gmet_2735

Alternate gene names: 78223931

Gene position: 3091132-3093084 (Clockwise)

Preceding gene: 78223929

Following gene: 78223932

Centisome position: 77.33

GC content: 63.34

Gene sequence:

>1953_bases
ATGCCCGAATCGCCATCAATCAACGACGTTATTGAATCCTCCCACCCCCCCTTGGGGAGCCGGGCAAATACGTCGTTGAT
TCGCATGGTTATCTCGGCACTTCTCGTCTGTTTCACCCTTCTTTTCACCGGTGCCCCCCCCCTCCACGCCGCAGAGGAGA
GTGACTCAAATTTGTTGGAGCTCTACTACGAGCCCAGCGATTTGGCGGTATCAGCCGCCACCCGCTCCCCCCGCCCCCTT
TCCCGCAGCGCCGAAGACATCACCGTGGTCACCGCCGCGGAGATCGAGGCCCAGAACGCCCATAACCTGGCAGAGGTGCT
GAACAGGGTCCCCGGGGTCCAGGTCGACCTGCGGGGTGGACTCGTCACCGACTACTCGCTGCTGGGCTCCAGTTTCCGCC
ATGTCCTGGTCCTCGTGGATGGCGTCTCCATGAGCAATCTGTCCGACAACGCCGCGGTCATCGCCCAGATCCCGGTGGGG
AACATCGAACGGATCGAAATCGTGAAGGGGCCGGCCTCCACGGCCTGGGGCTCCGCCCTGGGGGGGGTCATCAACGTTAT
CACTAAATCCCCCCGGGAAACTGCCATCCCCGGCGGGTCCGCCTCCTTCTCCGGGGGGGAGCGCGGCACCCGCGACGCCC
AAGGCGAGCTGACCGGGACCATGGGACGGCTGGGCTACTACCTCTCGGGCGGCAACGTCCGGTCCGACGGCTTCCGCCCC
CATAATGCCTCCGACAGCAACAACCTCTATGCCAAGCTCCTCTGGAGCCTCCGCGACAAGGGGAGCATCCGCTTCACCAT
CAACTACCTCAAGGGTGCAGCGGGCGAAGGGGTCTTCCCGCTCCCCGCCCGGCTGCTCCCCATTCTGTCCGAAGGCACAG
TCACCTCCGATTTAGAGACAAGCCGACTCACCTCGACCCTTGCCCTGAATTACCACCTCACCGAGCGGCTCGCCCTTGAC
CTTGCTCTCAAAGGCTCCCTCCTTGACGCGGATAGCAGGCGTTACTTCCTGACTTCCGGCGACACATTCCAACTCGTCGA
CAATAAGGAGGAAGCCGCCGGAGCGAGTGCTCTCCTCACGTGGCGGCAGGGGCTCCACACGGTGGTCGGCGGCATGGATT
TCGACCACGGCCACACAAAAACCAGTAGCGACACCTTCACCCTGCTCGGCACCTTCCCCGCGCCGAGCGAGGGGACCAAC
GACAAGTGGGGCTTCTTCCTGAACGACACCATCGCCTGGGAGCGGTTCACCCTCACCCCGGCGCTGCGTTATGACCGGAC
CAGCCGCGATGGCGAGTTCTGGAGCCAGAGCCTCGGGGCAACCGTCAACCTCACCGACCGGACCCTCCTGCGGGCCAACT
ACGCCCGGGGGTACAGCCTGTCGACCATCAATTTCGACCCGGCCTTCACCAACGAACGGATCTGGGCAATCCACGCGGGA
ATCGAGACGACGGAAATCCCCTTCCTCTGGCTCAAGGCGGGATGGTTCCGCAACACGGCCCGGGATGTCGCCGTCAACGG
TGAACGGCTGAGACTGCTCAAGGAAGGGTTCGAGGTGGAGGCCCGCACCACCCCCTTCTTTGATACCTGGCTCTCCCTGG
GCTACGTCTTCATCGACGCCAGGAACGCCGACACCGACCGGCGGCTTCAGGAAATTCCCCGCCAGACGTGGGACCTGGGG
CTCCACTATGACGACCGCACCTTCCGGGGCGCCCTCCTGGGGCACTATATCTGGTGGAACGAACCCTCGACGAGGGGCGG
CATTGCGTTCAATGCCCGCTACGATGACTTCATCTGGGACCTGAACCTGGGGTGGAAGGCCTACAAGGGTCAAAACGCCG
AAGCGGAGCTCTTCTTCACGGGCCACAACCTGTTCAACGGCAGGCAGTACACGAACGAAATCTTCGTCAACCCCAGCCGC
TGGTTCGAAGGCGGCCTCCGCATCAAGTGGTAA

Upstream 100 bases:

>100_bases
GGGTGACACAACAAAACTTTCCTGCTAAAAATCCGGAAAATTGTTCTCACCTCGCGCAACGTCAAATAACCAGGACCCAT
AACATTCACAAGCAACTCAC

Downstream 100 bases:

>100_bases
CGGAAACTATTTAGGAACGCATGAACCGACTGATCCTCCACATACTCGCTGTACTGCTCCTCTGGGGCTTCCCGGCTTTC
GCCGCGGAAGTCGTGGTGGT

Product: TonB-dependent receptor, plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 650; Mature: 649

Protein sequence:

>650_residues
MPESPSINDVIESSHPPLGSRANTSLIRMVISALLVCFTLLFTGAPPLHAAEESDSNLLELYYEPSDLAVSAATRSPRPL
SRSAEDITVVTAAEIEAQNAHNLAEVLNRVPGVQVDLRGGLVTDYSLLGSSFRHVLVLVDGVSMSNLSDNAAVIAQIPVG
NIERIEIVKGPASTAWGSALGGVINVITKSPRETAIPGGSASFSGGERGTRDAQGELTGTMGRLGYYLSGGNVRSDGFRP
HNASDSNNLYAKLLWSLRDKGSIRFTINYLKGAAGEGVFPLPARLLPILSEGTVTSDLETSRLTSTLALNYHLTERLALD
LALKGSLLDADSRRYFLTSGDTFQLVDNKEEAAGASALLTWRQGLHTVVGGMDFDHGHTKTSSDTFTLLGTFPAPSEGTN
DKWGFFLNDTIAWERFTLTPALRYDRTSRDGEFWSQSLGATVNLTDRTLLRANYARGYSLSTINFDPAFTNERIWAIHAG
IETTEIPFLWLKAGWFRNTARDVAVNGERLRLLKEGFEVEARTTPFFDTWLSLGYVFIDARNADTDRRLQEIPRQTWDLG
LHYDDRTFRGALLGHYIWWNEPSTRGGIAFNARYDDFIWDLNLGWKAYKGQNAEAELFFTGHNLFNGRQYTNEIFVNPSR
WFEGGLRIKW

Sequences:

>Translated_650_residues
MPESPSINDVIESSHPPLGSRANTSLIRMVISALLVCFTLLFTGAPPLHAAEESDSNLLELYYEPSDLAVSAATRSPRPL
SRSAEDITVVTAAEIEAQNAHNLAEVLNRVPGVQVDLRGGLVTDYSLLGSSFRHVLVLVDGVSMSNLSDNAAVIAQIPVG
NIERIEIVKGPASTAWGSALGGVINVITKSPRETAIPGGSASFSGGERGTRDAQGELTGTMGRLGYYLSGGNVRSDGFRP
HNASDSNNLYAKLLWSLRDKGSIRFTINYLKGAAGEGVFPLPARLLPILSEGTVTSDLETSRLTSTLALNYHLTERLALD
LALKGSLLDADSRRYFLTSGDTFQLVDNKEEAAGASALLTWRQGLHTVVGGMDFDHGHTKTSSDTFTLLGTFPAPSEGTN
DKWGFFLNDTIAWERFTLTPALRYDRTSRDGEFWSQSLGATVNLTDRTLLRANYARGYSLSTINFDPAFTNERIWAIHAG
IETTEIPFLWLKAGWFRNTARDVAVNGERLRLLKEGFEVEARTTPFFDTWLSLGYVFIDARNADTDRRLQEIPRQTWDLG
LHYDDRTFRGALLGHYIWWNEPSTRGGIAFNARYDDFIWDLNLGWKAYKGQNAEAELFFTGHNLFNGRQYTNEIFVNPSR
WFEGGLRIKW
>Mature_649_residues
PESPSINDVIESSHPPLGSRANTSLIRMVISALLVCFTLLFTGAPPLHAAEESDSNLLELYYEPSDLAVSAATRSPRPLS
RSAEDITVVTAAEIEAQNAHNLAEVLNRVPGVQVDLRGGLVTDYSLLGSSFRHVLVLVDGVSMSNLSDNAAVIAQIPVGN
IERIEIVKGPASTAWGSALGGVINVITKSPRETAIPGGSASFSGGERGTRDAQGELTGTMGRLGYYLSGGNVRSDGFRPH
NASDSNNLYAKLLWSLRDKGSIRFTINYLKGAAGEGVFPLPARLLPILSEGTVTSDLETSRLTSTLALNYHLTERLALDL
ALKGSLLDADSRRYFLTSGDTFQLVDNKEEAAGASALLTWRQGLHTVVGGMDFDHGHTKTSSDTFTLLGTFPAPSEGTND
KWGFFLNDTIAWERFTLTPALRYDRTSRDGEFWSQSLGATVNLTDRTLLRANYARGYSLSTINFDPAFTNERIWAIHAGI
ETTEIPFLWLKAGWFRNTARDVAVNGERLRLLKEGFEVEARTTPFFDTWLSLGYVFIDARNADTDRRLQEIPRQTWDLGL
HYDDRTFRGALLGHYIWWNEPSTRGGIAFNARYDDFIWDLNLGWKAYKGQNAEAELFFTGHNLFNGRQYTNEIFVNPSRW
FEGGLRIKW

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=160, Percent_Identity=33.75, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788478, Length=125, Percent_Identity=37.6, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787723, Length=242, Percent_Identity=28.099173553719, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 71808; Mature: 71677

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESPSINDVIESSHPPLGSRANTSLIRMVISALLVCFTLLFTGAPPLHAAEESDSNLLE
CCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
LYYEPSDLAVSAATRSPRPLSRSAEDITVVTAAEIEAQNAHNLAEVLNRVPGVQVDLRGG
EEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCC
LVTDYSLLGSSFRHVLVLVDGVSMSNLSDNAAVIAQIPVGNIERIEIVKGPASTAWGSAL
EEEHHHHHHHCCEEEEEEEECCEECCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHH
GGVINVITKSPRETAIPGGSASFSGGERGTRDAQGELTGTMGRLGYYLSGGNVRSDGFRP
HHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEECHHCCEEEEECCCCCCCCCCC
HNASDSNNLYAKLLWSLRDKGSIRFTINYLKGAAGEGVFPLPARLLPILSEGTVTSDLET
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCHH
SRLTSTLALNYHLTERLALDLALKGSLLDADSRRYFLTSGDTFQLVDNKEEAAGASALLT
HHHEEEEEEEEEEHHHHEEEEEECCCEECCCCCEEEEECCCEEEEECCCHHHCCHHHHHH
WRQGLHTVVGGMDFDHGHTKTSSDTFTLLGTFPAPSEGTNDKWGFFLNDTIAWERFTLTP
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECEEEEEEEEECH
ALRYDRTSRDGEFWSQSLGATVNLTDRTLLRANYARGYSLSTINFDPAFTNERIWAIHAG
HHHCCCCCCCHHHHHHHCCCEEEECCCEEEEECCCCCEEEEEEECCCCCCCCEEEEEECC
IETTEIPFLWLKAGWFRNTARDVAVNGERLRLLKEGFEVEARTTPFFDTWLSLGYVFIDA
CCCCCCCEEEEECCCCCCCCCCEEECCHHHHHHHCCCEEEECCCCHHHHHHCCCEEEEEC
RNADTDRRLQEIPRQTWDLGLHYDDRTFRGALLGHYIWWNEPSTRGGIAFNARYDDFIWD
CCCCHHHHHHHCCHHHHHCCEEECCCHHHHHHEEEEEEECCCCCCCCEEEECCCCCEEEE
LNLGWKAYKGQNAEAELFFTGHNLFNGRQYTNEIFVNPSRWFEGGLRIKW
ECCCEEEECCCCCCEEEEEECCCCCCCCEECCEEEECCHHHHCCCCEEEC
>Mature Secondary Structure 
PESPSINDVIESSHPPLGSRANTSLIRMVISALLVCFTLLFTGAPPLHAAEESDSNLLE
CCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEE
LYYEPSDLAVSAATRSPRPLSRSAEDITVVTAAEIEAQNAHNLAEVLNRVPGVQVDLRGG
EEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCC
LVTDYSLLGSSFRHVLVLVDGVSMSNLSDNAAVIAQIPVGNIERIEIVKGPASTAWGSAL
EEEHHHHHHHCCEEEEEEEECCEECCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHH
GGVINVITKSPRETAIPGGSASFSGGERGTRDAQGELTGTMGRLGYYLSGGNVRSDGFRP
HHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCEEECHHCCEEEEECCCCCCCCCCC
HNASDSNNLYAKLLWSLRDKGSIRFTINYLKGAAGEGVFPLPARLLPILSEGTVTSDLET
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCHH
SRLTSTLALNYHLTERLALDLALKGSLLDADSRRYFLTSGDTFQLVDNKEEAAGASALLT
HHHEEEEEEEEEEHHHHEEEEEECCCEECCCCCEEEEECCCEEEEECCCHHHCCHHHHHH
WRQGLHTVVGGMDFDHGHTKTSSDTFTLLGTFPAPSEGTNDKWGFFLNDTIAWERFTLTP
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECEEEEEEEEECH
ALRYDRTSRDGEFWSQSLGATVNLTDRTLLRANYARGYSLSTINFDPAFTNERIWAIHAG
HHHCCCCCCCHHHHHHHCCCEEEECCCEEEEECCCCCEEEEEEECCCCCCCCEEEEEECC
IETTEIPFLWLKAGWFRNTARDVAVNGERLRLLKEGFEVEARTTPFFDTWLSLGYVFIDA
CCCCCCCEEEEECCCCCCCCCCEEECCHHHHHHHCCCEEEECCCCHHHHHHCCCEEEEEC
RNADTDRRLQEIPRQTWDLGLHYDDRTFRGALLGHYIWWNEPSTRGGIAFNARYDDFIWD
CCCCHHHHHHHCCHHHHHCCEEECCCHHHHHHEEEEEEECCCCCCCCEEEECCCCCEEEE
LNLGWKAYKGQNAEAELFFTGHNLFNGRQYTNEIFVNPSRWFEGGLRIKW
ECCCEEEECCCCCCEEEEEECCCCCCCCEECCEEEECCHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA