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The map label for this gene is ligA

Identifier: 78223924

GI number: 78223924

Start: 3083292

End: 3085307

Strand: Direct

Name: ligA

Synonym: Gmet_2728

Alternate gene names: 78223924

Gene position: 3083292-3085307 (Clockwise)

Preceding gene: 304570572

Following gene: 78223925

Centisome position: 77.13

GC content: 66.07

Gene sequence:

>2016_bases
ATGGACAGAAACGCCGCCGAGCGGCGCATTGCTGAGTTGCGCGCCGAGATCAGGCGCCATGACCACCTCTATTACGTCCT
CGACCGCCCCGAGCTCACCGACGCGGAATACGACGCTCTCTATCGCGAACTGCTGAAGCTGGAAGAGGAGCATCCCGAGC
TGGTCACCCCCGACTCCCCTAGCCGGCGGGTGGGGGGGGCACCCCTGGAGAAGTTCAAGCAGGTGACCCACCGGATCCCG
ATGCTCTCACTGGAAAACGCCTTTACCGATGGCGATATCGCCGAGTTTGACGCCCGGGTGAAGCGCGCCCTGGCGCTTCC
GGCCGGGGAAGAGATCGCCTACGTGTGCGAACCGAAGCTGGACGGGCTTGCGGTGGAACTGGTCTATGAGTACGGCACGC
TCACCGTGGGCTCCACAAGGGGCGACGGGGTCGTGGGGGAGAACGTTACCCAGAACCTGAAAACCGTCAAGAGCATCCCC
CTGCGGCTCGAAGGGGAGAATCCCCCGGAGCTCCTGGAGGTGCGGGGCGAAGTGTTTCTCCCCCTGGCGGCCTTCCAGCG
GCTGAACGCCCAGCGGGAGGAGGAGGGAGAGCCCCCCTTCGCCAACCCCCGCAACGCGGCGGCGGGCTCCCTCCGGCAGT
TGGACTCGCGCATCACGGCCCGGCGCCCCCTCACCATGTTCTGCTATGCGCCGGGAGAGATTCGCGGCGCCGATTTCGGC
AGCCAAGGCGAGTTCCTCTCCGCCCTCCGGCAGCGGGGGCTTCCGGTGACCTCCCTGGCACGTCAGGTGACGGGAGTCGC
CGGGGTCCTCGCCTACTATCGCGAAATGACCGAAAAGCGCGACACCCTCCCCTACGAGATCGACGGGGTGGTGGTGAAGG
TGGACTCCTTTCCCCTCCAGCGAGAACTGGGAGAAAAGAGCCGCTCTCCCCGCTGGGCCGTGGCGGTAAAGTTCCCCCCC
CGCCAGGCGGTCACGGTCATCGAGGACATCGTCCCGTCCGTGGGGCGCACCGGGGTCATCACCCCCACGGCGAACCTGCG
CCCCGTGGAGGTTTCCGGGGTCACCGTCTCCCGGGCCACCCTCCACAACTGGGAGGAGATGGAGCGCAAGGACATCCGCA
TCGGCGACACGGTAGTGATCGAGCGGGCCGGCGACGTGATCCCGGCGGTGGTTAAGGTGCTGACGGAGAAACGCTCGGGG
AGCGAGCGGTTCCTCCCCATTCCCGCCGCCTGCCCCGAGTGCGGCTCCGAGGTGGTGAAGATCCCCGACGAGGTGGCGGT
CCGCTGCATGGGACTCTCCTGCCCGGCCCAGATCCGGGAGTCCATCATCCATTTCGCCTCCCGCAACGCCATGGACATGG
AGGGGCTCGGCGAGAAGTACATCGAGCAGCTCCTGCGGCTGGGGCTCGTGGGTAACGTGGCCGACCTCTACACCCTCACC
AGGGACGATTTCATGAAATTCGACCGGATGGGGGAGAAGCTGGCGGAGAACCTCCTGAACGCCATCGAGGCCAGCAAGAA
ACGGGAACTCTCCCGTTTCATCTTCGCCCTCGGCATCCGCCACGTGGGGGAGCACACGGCAAAGCTCCTGGCCACGGCCT
TCGGGAGCATCGACAACCTGGCCCGGGCCACGGAGGCGGAGCTCCTCTCCATCCGGGAGATCGGCCCCCAAGTGGCCCAA
AGCATCACCACCTTCTTCCACAACGAGGGGAACCGGGAGACCATCCGGCGGATGGTGGAGGCGGGGGTGGAGCCGACGGT
GGAAGAGAAAAAGGTGGGGGGAAAGTTCACCGGCAAGACCTTCGTCTTCACCGGTACGCTGATCCGCTTCAGCCGGAGCG
AGGCCCAGAAGATGGTGGAGAGCGAGGGGGGCCATGCGGCCGGGTCGGTATCGAAGAAGACCGACTACGTGGTGGCCGGG
GACGAAGCAGGGAGCAAGCTGGACAAGGCCCGGCAGCTGGGGGTCACGGTTCTCGCCGAGGATGAATTTCTGCAGATGCT
GGAGGGAGAGCAATGA

Upstream 100 bases:

>100_bases
TGTAACCAATCCCTGCACAGCCCTGTTAACCATCCCGACAAATTGCCATGACATCCCCGCTATGCTATAAGCACCTATCA
TCAAACCACGGAGATTCACC

Downstream 100 bases:

>100_bases
CCATGAAGATTCGCGCCATCGTCACCGTCAAGGGGCTTGTGCAGGGGGTCGCATTCCGCCATCACACGGTACTCCAGGGA
AATCAGCTCAGGGTGACCGG

Product: NAD-dependent DNA ligase C4-type

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MDRNAAERRIAELRAEIRRHDHLYYVLDRPELTDAEYDALYRELLKLEEEHPELVTPDSPSRRVGGAPLEKFKQVTHRIP
MLSLENAFTDGDIAEFDARVKRALALPAGEEIAYVCEPKLDGLAVELVYEYGTLTVGSTRGDGVVGENVTQNLKTVKSIP
LRLEGENPPELLEVRGEVFLPLAAFQRLNAQREEEGEPPFANPRNAAAGSLRQLDSRITARRPLTMFCYAPGEIRGADFG
SQGEFLSALRQRGLPVTSLARQVTGVAGVLAYYREMTEKRDTLPYEIDGVVVKVDSFPLQRELGEKSRSPRWAVAVKFPP
RQAVTVIEDIVPSVGRTGVITPTANLRPVEVSGVTVSRATLHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSG
SERFLPIPAACPECGSEVVKIPDEVAVRCMGLSCPAQIRESIIHFASRNAMDMEGLGEKYIEQLLRLGLVGNVADLYTLT
RDDFMKFDRMGEKLAENLLNAIEASKKRELSRFIFALGIRHVGEHTAKLLATAFGSIDNLARATEAELLSIREIGPQVAQ
SITTFFHNEGNRETIRRMVEAGVEPTVEEKKVGGKFTGKTFVFTGTLIRFSRSEAQKMVESEGGHAAGSVSKKTDYVVAG
DEAGSKLDKARQLGVTVLAEDEFLQMLEGEQ

Sequences:

>Translated_671_residues
MDRNAAERRIAELRAEIRRHDHLYYVLDRPELTDAEYDALYRELLKLEEEHPELVTPDSPSRRVGGAPLEKFKQVTHRIP
MLSLENAFTDGDIAEFDARVKRALALPAGEEIAYVCEPKLDGLAVELVYEYGTLTVGSTRGDGVVGENVTQNLKTVKSIP
LRLEGENPPELLEVRGEVFLPLAAFQRLNAQREEEGEPPFANPRNAAAGSLRQLDSRITARRPLTMFCYAPGEIRGADFG
SQGEFLSALRQRGLPVTSLARQVTGVAGVLAYYREMTEKRDTLPYEIDGVVVKVDSFPLQRELGEKSRSPRWAVAVKFPP
RQAVTVIEDIVPSVGRTGVITPTANLRPVEVSGVTVSRATLHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSG
SERFLPIPAACPECGSEVVKIPDEVAVRCMGLSCPAQIRESIIHFASRNAMDMEGLGEKYIEQLLRLGLVGNVADLYTLT
RDDFMKFDRMGEKLAENLLNAIEASKKRELSRFIFALGIRHVGEHTAKLLATAFGSIDNLARATEAELLSIREIGPQVAQ
SITTFFHNEGNRETIRRMVEAGVEPTVEEKKVGGKFTGKTFVFTGTLIRFSRSEAQKMVESEGGHAAGSVSKKTDYVVAG
DEAGSKLDKARQLGVTVLAEDEFLQMLEGEQ
>Mature_671_residues
MDRNAAERRIAELRAEIRRHDHLYYVLDRPELTDAEYDALYRELLKLEEEHPELVTPDSPSRRVGGAPLEKFKQVTHRIP
MLSLENAFTDGDIAEFDARVKRALALPAGEEIAYVCEPKLDGLAVELVYEYGTLTVGSTRGDGVVGENVTQNLKTVKSIP
LRLEGENPPELLEVRGEVFLPLAAFQRLNAQREEEGEPPFANPRNAAAGSLRQLDSRITARRPLTMFCYAPGEIRGADFG
SQGEFLSALRQRGLPVTSLARQVTGVAGVLAYYREMTEKRDTLPYEIDGVVVKVDSFPLQRELGEKSRSPRWAVAVKFPP
RQAVTVIEDIVPSVGRTGVITPTANLRPVEVSGVTVSRATLHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSG
SERFLPIPAACPECGSEVVKIPDEVAVRCMGLSCPAQIRESIIHFASRNAMDMEGLGEKYIEQLLRLGLVGNVADLYTLT
RDDFMKFDRMGEKLAENLLNAIEASKKRELSRFIFALGIRHVGEHTAKLLATAFGSIDNLARATEAELLSIREIGPQVAQ
SITTFFHNEGNRETIRRMVEAGVEPTVEEKKVGGKFTGKTFVFTGTLIRFSRSEAQKMVESEGGHAAGSVSKKTDYVVAG
DEAGSKLDKARQLGVTVLAEDEFLQMLEGEQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=668, Percent_Identity=48.3532934131737, Blast_Score=623, Evalue=1e-179,
Organism=Escherichia coli, GI87082305, Length=522, Percent_Identity=24.904214559387, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_GEOMG (Q39S28)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385671.1
- ProteinModelPortal:   Q39S28
- STRING:   Q39S28
- GeneID:   3740035
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2728
- NMPDR:   fig|269799.3.peg.2275
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q39S28
- ProtClustDB:   CLSK828084
- BioCyc:   GMET269799:GMET_2728-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74226; Mature: 74226

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 119-119 BINDING 117-117 BINDING 140-140 BINDING 177-177 BINDING 293-293 BINDING 317-317

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRNAAERRIAELRAEIRRHDHLYYVLDRPELTDAEYDALYRELLKLEEEHPELVTPDSP
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
SRRVGGAPLEKFKQVTHRIPMLSLENAFTDGDIAEFDARVKRALALPAGEEIAYVCEPKL
CCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCC
DGLAVELVYEYGTLTVGSTRGDGVVGENVTQNLKTVKSIPLRLEGENPPELLEVRGEVFL
CCEEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHCCCHHH
PLAAFQRLNAQREEEGEPPFANPRNAAAGSLRQLDSRITARRPLTMFCYAPGEIRGADFG
HHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
SQGEFLSALRQRGLPVTSLARQVTGVAGVLAYYREMTEKRDTLPYEIDGVVVKVDSFPLQ
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCHH
RELGEKSRSPRWAVAVKFPPRQAVTVIEDIVPSVGRTGVITPTANLRPVEVSGVTVSRAT
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCEEEEHHH
LHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSGSERFLPIPAACPECGSEVVK
HHCHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHCHHHHC
IPDEVAVRCMGLSCPAQIRESIIHFASRNAMDMEGLGEKYIEQLLRLGLVGNVADLYTLT
CCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCCHHHHHHHH
RDDFMKFDRMGEKLAENLLNAIEASKKRELSRFIFALGIRHVGEHTAKLLATAFGSIDNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARATEAELLSIREIGPQVAQSITTFFHNEGNRETIRRMVEAGVEPTVEEKKVGGKFTGKT
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCE
FVFTGTLIRFSRSEAQKMVESEGGHAAGSVSKKTDYVVAGDEAGSKLDKARQLGVTVLAE
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCEEEEH
DEFLQMLEGEQ
HHHHHHHCCCC
>Mature Secondary Structure
MDRNAAERRIAELRAEIRRHDHLYYVLDRPELTDAEYDALYRELLKLEEEHPELVTPDSP
CCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
SRRVGGAPLEKFKQVTHRIPMLSLENAFTDGDIAEFDARVKRALALPAGEEIAYVCEPKL
CCCCCCCCHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCC
DGLAVELVYEYGTLTVGSTRGDGVVGENVTQNLKTVKSIPLRLEGENPPELLEVRGEVFL
CCEEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHCCCHHH
PLAAFQRLNAQREEEGEPPFANPRNAAAGSLRQLDSRITARRPLTMFCYAPGEIRGADFG
HHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
SQGEFLSALRQRGLPVTSLARQVTGVAGVLAYYREMTEKRDTLPYEIDGVVVKVDSFPLQ
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCHH
RELGEKSRSPRWAVAVKFPPRQAVTVIEDIVPSVGRTGVITPTANLRPVEVSGVTVSRAT
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCEEEEHHH
LHNWEEMERKDIRIGDTVVIERAGDVIPAVVKVLTEKRSGSERFLPIPAACPECGSEVVK
HHCHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHCHHHHC
IPDEVAVRCMGLSCPAQIRESIIHFASRNAMDMEGLGEKYIEQLLRLGLVGNVADLYTLT
CCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHCCCHHHHHHHH
RDDFMKFDRMGEKLAENLLNAIEASKKRELSRFIFALGIRHVGEHTAKLLATAFGSIDNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARATEAELLSIREIGPQVAQSITTFFHNEGNRETIRRMVEAGVEPTVEEKKVGGKFTGKT
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCE
FVFTGTLIRFSRSEAQKMVESEGGHAAGSVSKKTDYVVAGDEAGSKLDKARQLGVTVLAE
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCEEEEH
DEFLQMLEGEQ
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA