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The map label for this gene is tilS/hprT [H]

Identifier: 78223912

GI number: 78223912

Start: 3068209

End: 3068766

Strand: Reverse

Name: tilS/hprT [H]

Synonym: Gmet_2716

Alternate gene names: 78223912

Gene position: 3068766-3068209 (Counterclockwise)

Preceding gene: 78223913

Following gene: 78223911

Centisome position: 76.77

GC content: 63.26

Gene sequence:

>558_bases
ATGTCCGTCACCCTCGACGAGGTAAAACAAGTCTACGCGGAGGCCGACTGTCTCTACACCGCTCCCCAGGTTGAAGAGAC
CATCGACCGGATGGCGCGGGAGATCACCGCACGGCTGGCCGATGCCAACCCCGTCGTCTTCTGCGTCATGAAGGGGGGGA
TCATCGTTACCGGCAAACTCCTGCCGAAGCTGGAATTCCCCCTGGAGGCGGAGTACCTCCACGCCACCCGTTACGGCCAC
AAGCTCTACGGCACCGTCCTCGACTGGAAGGTGCGGCCGACCAAGGAACTGAAGGACCGCACCGTCCTCATCATCGACGA
CATCCTGGACGAGGGGGAGACCCTGGGCGCCATTGCCGACTGGTGCCGGGGCATGGAGGCGAAGGAGGTGCTGACGGCCG
TGCTGATCGACAAGGAGCATGACCGCAAGGCCCGTCCCGACCTGGTCCCCGAGTTCGTGGGGCTCAGGACGCCGGATCGC
TATCTCTTCGGCTTCGGCATGGACTACAAGGGGTATTGGCGGAACGCACCGGGGATTTTCGCTCTCAAGGGGTACTGA

Upstream 100 bases:

>100_bases
CTACGGCGCGGCGTTTCTTGAGGCGATTCGGAACAATTCGTGAGTATTTGTTTCTGAGCGAAATTTTCATTTCCACTATT
TTCAACGCCCGGAGGTTCCC

Downstream 100 bases:

>100_bases
AACGCGGCACCGGCCCCTCACCCCCAACCCCTCTCCCCGGGGGAGAGGGTGGCCGCAAGGCCGGGTGAGGGGAATTGTTC
GGGGTTTTTTTCATACATGA

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSVTLDEVKQVYAEADCLYTAPQVEETIDRMAREITARLADANPVVFCVMKGGIIVTGKLLPKLEFPLEAEYLHATRYGH
KLYGTVLDWKVRPTKELKDRTVLIIDDILDEGETLGAIADWCRGMEAKEVLTAVLIDKEHDRKARPDLVPEFVGLRTPDR
YLFGFGMDYKGYWRNAPGIFALKGY

Sequences:

>Translated_185_residues
MSVTLDEVKQVYAEADCLYTAPQVEETIDRMAREITARLADANPVVFCVMKGGIIVTGKLLPKLEFPLEAEYLHATRYGH
KLYGTVLDWKVRPTKELKDRTVLIIDDILDEGETLGAIADWCRGMEAKEVLTAVLIDKEHDRKARPDLVPEFVGLRTPDR
YLFGFGMDYKGYWRNAPGIFALKGY
>Mature_184_residues
SVTLDEVKQVYAEADCLYTAPQVEETIDRMAREITARLADANPVVFCVMKGGIIVTGKLLPKLEFPLEAEYLHATRYGHK
LYGTVLDWKVRPTKELKDRTVLIIDDILDEGETLGAIADWCRGMEAKEVLTAVLIDKEHDRKARPDLVPEFVGLRTPDRY
LFGFGMDYKGYWRNAPGIFALKGY

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=164, Percent_Identity=26.219512195122, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI87081689, Length=162, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17510817, Length=160, Percent_Identity=25.625, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20986; Mature: 20854

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTLDEVKQVYAEADCLYTAPQVEETIDRMAREITARLADANPVVFCVMKGGIIVTGKL
CCCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECC
LPKLEFPLEAEYLHATRYGHKLYGTVLDWKVRPTKELKDRTVLIIDDILDEGETLGAIAD
CCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCHHHCCCEEEEEEHHHCCCCHHHHHHH
WCRGMEAKEVLTAVLIDKEHDRKARPDLVPEFVGLRTPDRYLFGFGMDYKGYWRNAPGIF
HHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCEE
ALKGY
EECCC
>Mature Secondary Structure 
SVTLDEVKQVYAEADCLYTAPQVEETIDRMAREITARLADANPVVFCVMKGGIIVTGKL
CCCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECC
LPKLEFPLEAEYLHATRYGHKLYGTVLDWKVRPTKELKDRTVLIIDDILDEGETLGAIAD
CCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCHHHCCCEEEEEEHHHCCCCHHHHHHH
WCRGMEAKEVLTAVLIDKEHDRKARPDLVPEFVGLRTPDRYLFGFGMDYKGYWRNAPGIF
HHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCEE
ALKGY
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]