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The map label for this gene is ywlF [H]

Identifier: 78223818

GI number: 78223818

Start: 2966066

End: 2966518

Strand: Reverse

Name: ywlF [H]

Synonym: Gmet_2621

Alternate gene names: 78223818

Gene position: 2966518-2966066 (Counterclockwise)

Preceding gene: 78223819

Following gene: 78223817

Centisome position: 74.21

GC content: 66.67

Gene sequence:

>453_bases
ATGCGGATAGGCATCGCCGCCGACCACGGCGGTTTCACCATGAAGGAGCGGATGGCTGCCGCCCTCAGGGAGGCGGGGTA
CGAGTTGACGGACTTCGGCGCTGAACGGCTGGACCCGGACGATGATTACCCCGATTTCGTCATCCCCTTGGCGCAAGCGG
TTGGCGAAGGGAAGCTGGATCGGGGGATCGCCTTCTGCTCGAGCGGCGTGGGGGCATCCATTGCCGCCAACAAGGTCCCC
GGAGCACGGGCGGCCCTGATTCACGACGACTACTCCGCCCACCAGGGTGTGGAGCACGACGACATGAACATCATCTGCCT
CGGCAGTCTGGTGGTGGGGTACGCTCAATCCTGGGAGCTGGTCAGGGCCTTCCTCGCCGCCACCTTCAGCGGGGCCGAGC
GCCACCGTCGCCGCCTGGCCAAGGTGGCGGCCCTGGAACAGTGCGGGAGGTGA

Upstream 100 bases:

>100_bases
GACAGCCCCTGGCTGACAAGGGCAGATCCTTACAAATTGTTGAAGAGATGACCCTGAAACTTACGCCGCTCATTCTGAAA
AGTGGCCAGGAGTGACGACC

Downstream 100 bases:

>100_bases
CCGATGCAAATTGGCATGATTGGCCTTGGACGGATGGGTGCGGATATGGTGCGGCGCCTGCGGAAGGGGGGACATGAGTG
CGTCGTGTATGATCTGAATG

Product: ribose/galactose isomerase

Products: D-Ribulose 5-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MRIGIAADHGGFTMKERMAAALREAGYELTDFGAERLDPDDDYPDFVIPLAQAVGEGKLDRGIAFCSSGVGASIAANKVP
GARAALIHDDYSAHQGVEHDDMNIICLGSLVVGYAQSWELVRAFLAATFSGAERHRRRLAKVAALEQCGR

Sequences:

>Translated_150_residues
MRIGIAADHGGFTMKERMAAALREAGYELTDFGAERLDPDDDYPDFVIPLAQAVGEGKLDRGIAFCSSGVGASIAANKVP
GARAALIHDDYSAHQGVEHDDMNIICLGSLVVGYAQSWELVRAFLAATFSGAERHRRRLAKVAALEQCGR
>Mature_150_residues
MRIGIAADHGGFTMKERMAAALREAGYELTDFGAERLDPDDDYPDFVIPLAQAVGEGKLDRGIAFCSSGVGASIAANKVP
GARAALIHDDYSAHQGVEHDDMNIICLGSLVVGYAQSWELVRAFLAATFSGAERHRRRLAKVAALEQCGR

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=146, Percent_Identity=39.7260273972603, Blast_Score=102, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6 [C]

Molecular weight: Translated: 16077; Mature: 16077

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIAADHGGFTMKERMAAALREAGYELTDFGAERLDPDDDYPDFVIPLAQAVGEGKLD
CEEEEECCCCCCHHHHHHHHHHHHCCCCHHHCCHHHCCCCCCCCHHHHHHHHHHCCCCHH
RGIAFCSSGVGASIAANKVPGARAALIHDDYSAHQGVEHDDMNIICLGSLVVGYAQSWEL
HHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCEEEHHHHHHHHHHHHHH
VRAFLAATFSGAERHRRRLAKVAALEQCGR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGIAADHGGFTMKERMAAALREAGYELTDFGAERLDPDDDYPDFVIPLAQAVGEGKLD
CEEEEECCCCCCHHHHHHHHHHHHCCCCHHHCCHHHCCCCCCCCHHHHHHHHHHCCCCHH
RGIAFCSSGVGASIAANKVPGARAALIHDDYSAHQGVEHDDMNIICLGSLVVGYAQSWEL
HHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCEEEHHHHHHHHHHHHHH
VRAFLAATFSGAERHRRRLAKVAALEQCGR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: alpha-D-Ribose 5-phosphate [C]

Specific reaction: alpha-D-Ribose 5-phosphate <==> D-Ribulose 5-phosphate [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]