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The map label for this gene is hyfB [H]

Identifier: 78223798

GI number: 78223798

Start: 2950996

End: 2952978

Strand: Reverse

Name: hyfB [H]

Synonym: Gmet_2601

Alternate gene names: 78223798

Gene position: 2952978-2950996 (Counterclockwise)

Preceding gene: 78223802

Following gene: 78223797

Centisome position: 73.87

GC content: 60.56

Gene sequence:

>1983_bases
ATGTTCCTCCTTGATCCAACGTTATTTCTACTGGTTGCGGCGTCCTGTGTTGCTCTCTCGGGTATTCCGGGGCTGTTGGC
GCGCCGCTCTGCCTGGGGGCAACGGATCGCGGCCATACTTGTAATCGCTGCGTCGTGCCTTGGCGAGGTGGCTGTCATCT
CGCTCCTGTCCGGCATTCCCGCTTCATCATACCAGATTGACTGGCCCCTTCCTTTTGGACCGGCGCTCTTTTCCGCCGAT
CATCTTTCCACAATATTCCTCTTTCCGCTCTTCCTGGTTACCGGTGCTGTCGCTCTGTATTCCGTGGCCTACTGGCCGGC
CAGGGAAAAGGCGAGCGCCGGAAGGCTCACCTTCTTTCTCGGACTCTTTGCCGCCGCGATGATCATCGTGGTCATGGCCC
GTAACGGGGTGCTGTTTCTCATGGCCTGGGAGGTCATGGCGCTCTCTGCCTATTTCCTGCTGACCACCGAGCAGCAGAGC
TCCGATGTTCAGCGAGTCGGAACGGTCTACCTTCTTGCCACCCACACCGGCACCATGGCGCTGTTTGTCATGTTTTCCCT
GCTGCGCGGGACTGTCGGCACGTTCGCGTTTCCAGCCACGCATGCCCTTTCCTTGACCGTGCCTGTTGCGACAACGGTCA
TCATAACCGCCCTGATCGGGTTTGGCGGCAAGGCGGGCCTCATGCCCCTGCATTTCTGGCTTCCCGGCGCCCATGCCAAT
GCCCCCAGTCACGTTTCGGCGATGATGTCGGGGTTGATGCTCAAGATGGGTGTGTACGGCATCCTGCGGACCTTGTCGTT
TTTCGATCAGCTGCCGGTATGGCTCGGCTGGCTCATACTCCTGCTCGGTGCCTGGTCCGCCATTAACGGGATTGCCCTGG
CAGCAGGACAGCGCGACCTGAAACGCCTGCTGGCCTGCAGCAGTATCGAGAATATCGGCATTGTCTTTATCGGCATCGGC
CTGGCTCTGGTCGGCCTGAACCAGCACGCGCCATATCTGGTGGTGTGCGGCCTTGCGGGCGCCTTTATCCATATCGTCAA
CCATGGCCTGTTCAAGTCGCTCCTCTTCTTGGGAAGCGGGGTGCTGATACATGGCACCGGAACCCGGGAGATCGACCGGA
TGGGGGGGCTTGCCCGGCGCATGCCGGTAACATCGCCGCTCTTTCTGGTCGGGTCGCTCGCCATATGCGGTTTGCCGCCG
CTCAATGGATTTGTCGGCGAATTTTTTCTCTATATCGGTGCCATCACCGATGGCATCGTCGCTCCGTTGCCGCTGGCGGC
CCTGGTTGCGCCTGTCCTGGCGCTGGTTGGCGGGCTGGCGGTCCTGACCTTCGTCAAGCTTTTCGGGATCGTCTTTCTCG
GCTCTCCCCGCTCGGTCGAATCTGCCCATGGCCATGAAGCATCGGCGGTGATGACCGGACCAATGATGTTGCTGGCCGGC
GGATGCCTGGTTGCAGGTATGGCACCGGTGCTGCTGGTGCGGCTGGTTACCCCGGTTGTCGGCTTCTATGCCGGGCTCAC
CCCCCAGGATAGTGCCCAGGTTATGGGGCAGATCCCCCTTGTGGCGCTCACCTGCGCCAACGTGCTCCTTCTTGTGGTCA
TTCTGGCTGTCGGCGCGGCATACCGCCGGCGGCTGCGTACATCTCCCCGTTCCCAGGGAAGCACGTGGGGGTGCGGCTAT
CTCGAGCCTACACCCCGGATGCAATATACCGGCACATCGTTCAGCGAGATGGCGGTAAGCCTGCTGGGGAGTATCGTTGC
CCCCAACCGTCGCAGGCCCACGCTTGATACGCCCATTCCTCCCGCCGACGCCCGTTTCAGCTATGAGACAACCGAAACCG
TGCTGGACCGGATTCTGATTCCCGTCTTCCGGGTCGTCGGCACGGCATTTTCCTATCTCAGGAAAGTGCAGCACGGCCAG
CTTCATATTTACGTGTTGTATATATTCGCCACGCTCTGCGTCCTGATGATCTGGAAGCACTGA

Upstream 100 bases:

>100_bases
AAAGTGTTATAAAAATGTAGTAACTCATAATGTTTTTGTGTCGCCCAGTAATCAAGTCAGCAGTTGACGTGAGATAGGGG
GCAGGTGTGAGGCAGGCGTG

Downstream 100 bases:

>100_bases
ACGAGGACAGCGGCGTTCCCGTCTCCTTCGAGGATGGCACGTTATGACAGACATTATCATCCACTGCGCAGTGGCGCTCC
TGTTGCCGCCACTGTTGCTC

Product: NADH/ubiquinone/plastoquinone (complex I)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MFLLDPTLFLLVAASCVALSGIPGLLARRSAWGQRIAAILVIAASCLGEVAVISLLSGIPASSYQIDWPLPFGPALFSAD
HLSTIFLFPLFLVTGAVALYSVAYWPAREKASAGRLTFFLGLFAAAMIIVVMARNGVLFLMAWEVMALSAYFLLTTEQQS
SDVQRVGTVYLLATHTGTMALFVMFSLLRGTVGTFAFPATHALSLTVPVATTVIITALIGFGGKAGLMPLHFWLPGAHAN
APSHVSAMMSGLMLKMGVYGILRTLSFFDQLPVWLGWLILLLGAWSAINGIALAAGQRDLKRLLACSSIENIGIVFIGIG
LALVGLNQHAPYLVVCGLAGAFIHIVNHGLFKSLLFLGSGVLIHGTGTREIDRMGGLARRMPVTSPLFLVGSLAICGLPP
LNGFVGEFFLYIGAITDGIVAPLPLAALVAPVLALVGGLAVLTFVKLFGIVFLGSPRSVESAHGHEASAVMTGPMMLLAG
GCLVAGMAPVLLVRLVTPVVGFYAGLTPQDSAQVMGQIPLVALTCANVLLLVVILAVGAAYRRRLRTSPRSQGSTWGCGY
LEPTPRMQYTGTSFSEMAVSLLGSIVAPNRRRPTLDTPIPPADARFSYETTETVLDRILIPVFRVVGTAFSYLRKVQHGQ
LHIYVLYIFATLCVLMIWKH

Sequences:

>Translated_660_residues
MFLLDPTLFLLVAASCVALSGIPGLLARRSAWGQRIAAILVIAASCLGEVAVISLLSGIPASSYQIDWPLPFGPALFSAD
HLSTIFLFPLFLVTGAVALYSVAYWPAREKASAGRLTFFLGLFAAAMIIVVMARNGVLFLMAWEVMALSAYFLLTTEQQS
SDVQRVGTVYLLATHTGTMALFVMFSLLRGTVGTFAFPATHALSLTVPVATTVIITALIGFGGKAGLMPLHFWLPGAHAN
APSHVSAMMSGLMLKMGVYGILRTLSFFDQLPVWLGWLILLLGAWSAINGIALAAGQRDLKRLLACSSIENIGIVFIGIG
LALVGLNQHAPYLVVCGLAGAFIHIVNHGLFKSLLFLGSGVLIHGTGTREIDRMGGLARRMPVTSPLFLVGSLAICGLPP
LNGFVGEFFLYIGAITDGIVAPLPLAALVAPVLALVGGLAVLTFVKLFGIVFLGSPRSVESAHGHEASAVMTGPMMLLAG
GCLVAGMAPVLLVRLVTPVVGFYAGLTPQDSAQVMGQIPLVALTCANVLLLVVILAVGAAYRRRLRTSPRSQGSTWGCGY
LEPTPRMQYTGTSFSEMAVSLLGSIVAPNRRRPTLDTPIPPADARFSYETTETVLDRILIPVFRVVGTAFSYLRKVQHGQ
LHIYVLYIFATLCVLMIWKH
>Mature_660_residues
MFLLDPTLFLLVAASCVALSGIPGLLARRSAWGQRIAAILVIAASCLGEVAVISLLSGIPASSYQIDWPLPFGPALFSAD
HLSTIFLFPLFLVTGAVALYSVAYWPAREKASAGRLTFFLGLFAAAMIIVVMARNGVLFLMAWEVMALSAYFLLTTEQQS
SDVQRVGTVYLLATHTGTMALFVMFSLLRGTVGTFAFPATHALSLTVPVATTVIITALIGFGGKAGLMPLHFWLPGAHAN
APSHVSAMMSGLMLKMGVYGILRTLSFFDQLPVWLGWLILLLGAWSAINGIALAAGQRDLKRLLACSSIENIGIVFIGIG
LALVGLNQHAPYLVVCGLAGAFIHIVNHGLFKSLLFLGSGVLIHGTGTREIDRMGGLARRMPVTSPLFLVGSLAICGLPP
LNGFVGEFFLYIGAITDGIVAPLPLAALVAPVLALVGGLAVLTFVKLFGIVFLGSPRSVESAHGHEASAVMTGPMMLLAG
GCLVAGMAPVLLVRLVTPVVGFYAGLTPQDSAQVMGQIPLVALTCANVLLLVVILAVGAAYRRRLRTSPRSQGSTWGCGY
LEPTPRMQYTGTSFSEMAVSLLGSIVAPNRRRPTLDTPIPPADARFSYETTETVLDRILIPVFRVVGTAFSYLRKVQHGQ
LHIYVLYIFATLCVLMIWKH

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=431, Percent_Identity=25.9860788863109, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI251831116, Length=300, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1788827, Length=610, Percent_Identity=34.7540983606557, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI2367154, Length=498, Percent_Identity=33.3333333333333, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1788831, Length=355, Percent_Identity=32.3943661971831, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1788829, Length=384, Percent_Identity=29.6875, Blast_Score=129, Evalue=8e-31,
Organism=Escherichia coli, GI1788613, Length=378, Percent_Identity=29.6296296296296, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788614, Length=392, Percent_Identity=30.3571428571429, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 70161; Mature: 70161

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLLDPTLFLLVAASCVALSGIPGLLARRSAWGQRIAAILVIAASCLGEVAVISLLSGIP
CCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASSYQIDWPLPFGPALFSADHLSTIFLFPLFLVTGAVALYSVAYWPAREKASAGRLTFFL
CCCEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
GLFAAAMIIVVMARNGVLFLMAWEVMALSAYFLLTTEQQSSDVQRVGTVYLLATHTGTMA
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHEEEECCHHHHHHHHCEEEEEEECCHHHH
LFVMFSLLRGTVGTFAFPATHALSLTVPVATTVIITALIGFGGKAGLMPLHFWLPGAHAN
HHHHHHHHHHHHHHHCCCCHHHEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
APSHVSAMMSGLMLKMGVYGILRTLSFFDQLPVWLGWLILLLGAWSAINGIALAAGQRDL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHH
KRLLACSSIENIGIVFIGIGLALVGLNQHAPYLVVCGLAGAFIHIVNHGLFKSLLFLGSG
HHHHHHHCCCCCCCCEEEHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
VLIHGTGTREIDRMGGLARRMPVTSPLFLVGSLAICGLPPLNGFVGEFFLYIGAITDGIV
EEEECCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHH
APLPLAALVAPVLALVGGLAVLTFVKLFGIVFLGSPRSVESAHGHEASAVMTGPMMLLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCHHHHHHH
GCLVAGMAPVLLVRLVTPVVGFYAGLTPQDSAQVMGQIPLVALTCANVLLLVVILAVGAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YRRRLRTSPRSQGSTWGCGYLEPTPRMQYTGTSFSEMAVSLLGSIVAPNRRRPTLDTPIP
HHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
PADARFSYETTETVLDRILIPVFRVVGTAFSYLRKVQHGQLHIYVLYIFATLCVLMIWKH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFLLDPTLFLLVAASCVALSGIPGLLARRSAWGQRIAAILVIAASCLGEVAVISLLSGIP
CCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASSYQIDWPLPFGPALFSADHLSTIFLFPLFLVTGAVALYSVAYWPAREKASAGRLTFFL
CCCEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
GLFAAAMIIVVMARNGVLFLMAWEVMALSAYFLLTTEQQSSDVQRVGTVYLLATHTGTMA
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHEEEECCHHHHHHHHCEEEEEEECCHHHH
LFVMFSLLRGTVGTFAFPATHALSLTVPVATTVIITALIGFGGKAGLMPLHFWLPGAHAN
HHHHHHHHHHHHHHHCCCCHHHEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
APSHVSAMMSGLMLKMGVYGILRTLSFFDQLPVWLGWLILLLGAWSAINGIALAAGQRDL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHH
KRLLACSSIENIGIVFIGIGLALVGLNQHAPYLVVCGLAGAFIHIVNHGLFKSLLFLGSG
HHHHHHHCCCCCCCCEEEHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
VLIHGTGTREIDRMGGLARRMPVTSPLFLVGSLAICGLPPLNGFVGEFFLYIGAITDGIV
EEEECCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHH
APLPLAALVAPVLALVGGLAVLTFVKLFGIVFLGSPRSVESAHGHEASAVMTGPMMLLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCHHHHHHH
GCLVAGMAPVLLVRLVTPVVGFYAGLTPQDSAQVMGQIPLVALTCANVLLLVVILAVGAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YRRRLRTSPRSQGSTWGCGYLEPTPRMQYTGTSFSEMAVSLLGSIVAPNRRRPTLDTPIP
HHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
PADARFSYETTETVLDRILIPVFRVVGTAFSYLRKVQHGQLHIYVLYIFATLCVLMIWKH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]