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The map label for this gene is ntrC [H]

Identifier: 78223760

GI number: 78223760

Start: 2909886

End: 2911328

Strand: Direct

Name: ntrC [H]

Synonym: Gmet_2563

Alternate gene names: 78223760

Gene position: 2909886-2911328 (Clockwise)

Preceding gene: 78223759

Following gene: 78223761

Centisome position: 72.79

GC content: 62.58

Gene sequence:

>1443_bases
ATGTTATTGCACCGCATACTCGTAGCCGACGACGAAGAAAGCATGCGCTGGGTCCTCTCCAAGGCCCTGCGGAAAAAGGG
ATTTTCCGTGGATCTCGCCCGGGACGGCGAAGAGGCCCTGCGCCTCATCCAGTCCGGCGACTATGATCTGGCGATCCTTG
ACATCAAGATGCCCGGCATTTCGGGGCTCGACCTCCTGGACCGGGTGAAGGAGCTGAAGAGCGACCTCCTCATGGTGATC
ATGACCGCCGAGGCAAGCATGAAGAACGCCGTGGAGGCCATGAAGCGCGGCGCCTACGACTACATCACCAAGCCCTTCGA
CCTGGACGTGATCGACGCCATCATCGAGAAGGTGAACCGGGCCAGGGAGATCACCTCCCAGATGTCGATCCTCCGGGAGG
AACTGAAGGACCGCTATCTCCTGGAGAAGAACATCGTCGGTAACTCCCCGGCCATGCGGGAGGTCTACAAGACCATCGGC
AAGGTGGCTCCCAGCGACGTAACGGTGCTGGTGCAGGGGGAGTCGGGGACCGGCAAGGAGCTCATCGCCCGGGCCATCCA
TTTCAACTCCAAACGGATCGGCAAGCCGTTCATTGCCCTCAACTGCGCAGCGATCCCCAAGGAACTCCTGGAGAGCGAGC
TCTTCGGCTTCGAGAAGGGGGCCTTCACCGGCGCCGCGGAGCGCAAACTCGGCAAGTTCGAGCAGGCCAACGGCGGCACT
ATCTTCCTGGACGAGATCGGCGACATGCCCCTGGACCTCCAGGCGAAGATCCTGCGGGTCCTCCAGGAGAAGGAGGTGAC
CCGCACCGGTGGGAGCCAGAACATCGCCGTGGACGTGCGGATCGTGGCGGCCACCAACCAGAACCTGGAGGAACTGGCCC
GGAACAAACAGTTCCGGGAAGACCTCTACTACCGCCTGAACGTGGTGCCCATCCAGCTGGTGCCGCTGCGGGAGCGCAAG
GAGGACATCCCGGCCCTGGTGGAGTACTTCCTCCACAAGACCTGTGCCGAACTGGAGGTCCCCGGCAGGACCTGCTCCCC
CGAGACCATGGCGCTCCTCACCGCCTACTCCTGGCCCGGCAACGTGCGGGAGCTGGAGAACACCATCAAAAGGGCCGTGA
TCCTCTCCTCCGACCCGCTCCTCACGCCGGCAGACTTTCCGGGGCTCCGCATCCAGCGCACCGGCGAGGCCGTCGCCACC
GACGAGCTGTCGCTTGAGGCCCTGGTGGACATGAAGCTGCGGGCGAGCCTCACCAATCTCGACAAGATGGAGACCGGCGA
CATCTACAACCTGGTCCTCAAACAGATCGAACGCCCCCTCATCCGCTTCGTCCTGGAAAAGACCCGGGGGAACCAAGTGA
AGGGCGCCGAAATCCTCGGCATCAACCGGAACACACTCCGGAAGAAGATCCAGGAACTGGGCATCGAAATCAGGAGAGAC
TGA

Upstream 100 bases:

>100_bases
TAACCAGGGACCAGGAACCGGGGACCAGTGGAATCAAAGTCAAGCGACTTTCGGTTTTACCGGTCCCCGGTCCCCGGTCC
CGGATTTCCGAGGTTTTATC

Downstream 100 bases:

>100_bases
AAGCCGGGACCGGGGATCAGGGACCGGGGATCAGGAAAAATCAAAAACAGCAACAAGCGAATCGGTACCATGGCCATCTA
CGGAGCATTACCGGGTTTTC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLLHRILVADDEESMRWVLSKALRKKGFSVDLARDGEEALRLIQSGDYDLAILDIKMPGISGLDLLDRVKELKSDLLMVI
MTAEASMKNAVEAMKRGAYDYITKPFDLDVIDAIIEKVNRAREITSQMSILREELKDRYLLEKNIVGNSPAMREVYKTIG
KVAPSDVTVLVQGESGTGKELIARAIHFNSKRIGKPFIALNCAAIPKELLESELFGFEKGAFTGAAERKLGKFEQANGGT
IFLDEIGDMPLDLQAKILRVLQEKEVTRTGGSQNIAVDVRIVAATNQNLEELARNKQFREDLYYRLNVVPIQLVPLRERK
EDIPALVEYFLHKTCAELEVPGRTCSPETMALLTAYSWPGNVRELENTIKRAVILSSDPLLTPADFPGLRIQRTGEAVAT
DELSLEALVDMKLRASLTNLDKMETGDIYNLVLKQIERPLIRFVLEKTRGNQVKGAEILGINRNTLRKKIQELGIEIRRD

Sequences:

>Translated_480_residues
MLLHRILVADDEESMRWVLSKALRKKGFSVDLARDGEEALRLIQSGDYDLAILDIKMPGISGLDLLDRVKELKSDLLMVI
MTAEASMKNAVEAMKRGAYDYITKPFDLDVIDAIIEKVNRAREITSQMSILREELKDRYLLEKNIVGNSPAMREVYKTIG
KVAPSDVTVLVQGESGTGKELIARAIHFNSKRIGKPFIALNCAAIPKELLESELFGFEKGAFTGAAERKLGKFEQANGGT
IFLDEIGDMPLDLQAKILRVLQEKEVTRTGGSQNIAVDVRIVAATNQNLEELARNKQFREDLYYRLNVVPIQLVPLRERK
EDIPALVEYFLHKTCAELEVPGRTCSPETMALLTAYSWPGNVRELENTIKRAVILSSDPLLTPADFPGLRIQRTGEAVAT
DELSLEALVDMKLRASLTNLDKMETGDIYNLVLKQIERPLIRFVLEKTRGNQVKGAEILGINRNTLRKKIQELGIEIRRD
>Mature_480_residues
MLLHRILVADDEESMRWVLSKALRKKGFSVDLARDGEEALRLIQSGDYDLAILDIKMPGISGLDLLDRVKELKSDLLMVI
MTAEASMKNAVEAMKRGAYDYITKPFDLDVIDAIIEKVNRAREITSQMSILREELKDRYLLEKNIVGNSPAMREVYKTIG
KVAPSDVTVLVQGESGTGKELIARAIHFNSKRIGKPFIALNCAAIPKELLESELFGFEKGAFTGAAERKLGKFEQANGGT
IFLDEIGDMPLDLQAKILRVLQEKEVTRTGGSQNIAVDVRIVAATNQNLEELARNKQFREDLYYRLNVVPIQLVPLRERK
EDIPALVEYFLHKTCAELEVPGRTCSPETMALLTAYSWPGNVRELENTIKRAVILSSDPLLTPADFPGLRIQRTGEAVAT
DELSLEALVDMKLRASLTNLDKMETGDIYNLVLKQIERPLIRFVLEKTRGNQVKGAEILGINRNTLRKKIQELGIEIRRD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=478, Percent_Identity=43.5146443514644, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1790299, Length=482, Percent_Identity=43.3609958506224, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1790437, Length=465, Percent_Identity=42.5806451612903, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI1788905, Length=464, Percent_Identity=37.7155172413793, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082117, Length=338, Percent_Identity=46.1538461538462, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1789087, Length=335, Percent_Identity=44.1791044776119, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789233, Length=336, Percent_Identity=45.8333333333333, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1786524, Length=336, Percent_Identity=44.047619047619, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=49.3617021276596, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=39.3846153846154, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1787583, Length=336, Percent_Identity=39.2857142857143, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1789828, Length=287, Percent_Identity=31.3588850174216, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081858, Length=330, Percent_Identity=29.6969696969697, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786784, Length=102, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=36.8852459016393, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=186, Percent_Identity=29.5698924731183, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789402, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082012, Length=103, Percent_Identity=33.0097087378641, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787375, Length=116, Percent_Identity=32.7586206896552, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789809, Length=104, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=33.8983050847458, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789661, Length=67, Percent_Identity=41.7910447761194, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1790552, Length=116, Percent_Identity=29.3103448275862, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=113, Percent_Identity=30.0884955752212, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53888; Mature: 53888

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLHRILVADDEESMRWVLSKALRKKGFSVDLARDGEEALRLIQSGDYDLAILDIKMPGI
CCEEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCEEEEEEECCCC
SGLDLLDRVKELKSDLLMVIMTAEASMKNAVEAMKRGAYDYITKPFDLDVIDAIIEKVNR
CCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHH
AREITSQMSILREELKDRYLLEKNIVGNSPAMREVYKTIGKVAPSDVTVLVQGESGTGKE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHH
LIARAIHFNSKRIGKPFIALNCAAIPKELLESELFGFEKGAFTGAAERKLGKFEQANGGT
HHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCE
IFLDEIGDMPLDLQAKILRVLQEKEVTRTGGSQNIAVDVRIVAATNQNLEELARNKQFRE
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
DLYYRLNVVPIQLVPLRERKEDIPALVEYFLHKTCAELEVPGRTCSPETMALLTAYSWPG
HHHEEEEEEEEEEEECHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEECCCC
NVRELENTIKRAVILSSDPLLTPADFPGLRIQRTGEAVATDELSLEALVDMKLRASLTNL
CHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
DKMETGDIYNLVLKQIERPLIRFVLEKTRGNQVKGAEILGINRNTLRKKIQELGIEIRRD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCEECCC
>Mature Secondary Structure
MLLHRILVADDEESMRWVLSKALRKKGFSVDLARDGEEALRLIQSGDYDLAILDIKMPGI
CCEEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCEEEEEEECCCC
SGLDLLDRVKELKSDLLMVIMTAEASMKNAVEAMKRGAYDYITKPFDLDVIDAIIEKVNR
CCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHH
AREITSQMSILREELKDRYLLEKNIVGNSPAMREVYKTIGKVAPSDVTVLVQGESGTGKE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHH
LIARAIHFNSKRIGKPFIALNCAAIPKELLESELFGFEKGAFTGAAERKLGKFEQANGGT
HHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCE
IFLDEIGDMPLDLQAKILRVLQEKEVTRTGGSQNIAVDVRIVAATNQNLEELARNKQFRE
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
DLYYRLNVVPIQLVPLRERKEDIPALVEYFLHKTCAELEVPGRTCSPETMALLTAYSWPG
HHHEEEEEEEEEEEECHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEEECCCC
NVRELENTIKRAVILSSDPLLTPADFPGLRIQRTGEAVATDELSLEALVDMKLRASLTNL
CHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
DKMETGDIYNLVLKQIERPLIRFVLEKTRGNQVKGAEILGINRNTLRKKIQELGIEIRRD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]