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The map label for this gene is trpA

Identifier: 78223680

GI number: 78223680

Start: 2817743

End: 2818552

Strand: Reverse

Name: trpA

Synonym: Gmet_2477

Alternate gene names: 78223680

Gene position: 2818552-2817743 (Counterclockwise)

Preceding gene: 78223681

Following gene: 78223679

Centisome position: 70.51

GC content: 61.36

Gene sequence:

>810_bases
ATGAGTAGAATTACCGGTACATTTGCCGAGTTGAAACGAACCGGAAAAAAGGCCTTGGTGACCTTTATCACGGCCGGTGA
TCCGGACCTTGCAACGACAGAAGAGCTGATACCGCTCCTGGCGGAGAACGGCGCCGACATCATCGAGCTGGGGGTACCCT
TTTCCGACCCCATGGCCGATGGCCCCACGATCCAACTCTCCTCCGAGCGTGCCCTTGCCGCCGGCACAACCCTGTCGAGG
ATTTTAGCTACAGTAAAATCCGTCCGGGGTCGCACCCAGGTCCCCATCGTCCTGATGGGATACTTCAACCCGATCTTCAG
CTACGGCCTCGAACGGTTTGCCGCCGATGCGGCCGCGGCAGGGGTCGACGGCGTCCTGCTGGTGGATCTTCCCCCCGAAG
AGGCGGGGGAGTTCAAGGCGTGCGCCGACCGGCACGGGATCGACGTCATTTTCCTCCTCACTCCCACGTCCGACGAGACG
CGGATCCGCACGGTGACCAACCGTGCCCGTGGCTTCATCTACTATGTCTCCGTAACCGGCGTGACCGGTGTCCGCAGCGG
CATAGAGGCATCGGTGGCCGGCAACGTGAACATCATCAAGGAGTGCTCTAAGGTGCCGGTGGCAGTAGGATTCGGTATCG
CCACCCCCGAACAGGCGGGGGAGGTGGCTGCCACGGCCGACGGCGTGGTGGTCGGCAGCGCCATTGTGAAGCTTTTCGAG
AAGCACCGGGGTGAGGAACTCAAGGGAGCACTGGCAACCTTTGTCTCATCGCTGAAGCAGGCAGTGGACCGGGGAAGATA
CGACAATTGA

Upstream 100 bases:

>100_bases
TTCAGGGAGAAATGAACAGCTTCAAGGTTGAAAATCAGGGAGCAGTGCCCAAGGGTTAGGGCCAGCAATGTAGTTGTCAC
CACCTCCGAAAGGTTTAACA

Downstream 100 bases:

>100_bases
CTTTGTCGTCGATTTCTGGTACCAGAGGACTTCATTTTTACTAAAAATATCCGGGGCGTTGCTTGTCCCGACGGAGTGAA
AGATATGGCGTGGTTCAAAC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSRITGTFAELKRTGKKALVTFITAGDPDLATTEELIPLLAENGADIIELGVPFSDPMADGPTIQLSSERALAAGTTLSR
ILATVKSVRGRTQVPIVLMGYFNPIFSYGLERFAADAAAAGVDGVLLVDLPPEEAGEFKACADRHGIDVIFLLTPTSDET
RIRTVTNRARGFIYYVSVTGVTGVRSGIEASVAGNVNIIKECSKVPVAVGFGIATPEQAGEVAATADGVVVGSAIVKLFE
KHRGEELKGALATFVSSLKQAVDRGRYDN

Sequences:

>Translated_269_residues
MSRITGTFAELKRTGKKALVTFITAGDPDLATTEELIPLLAENGADIIELGVPFSDPMADGPTIQLSSERALAAGTTLSR
ILATVKSVRGRTQVPIVLMGYFNPIFSYGLERFAADAAAAGVDGVLLVDLPPEEAGEFKACADRHGIDVIFLLTPTSDET
RIRTVTNRARGFIYYVSVTGVTGVRSGIEASVAGNVNIIKECSKVPVAVGFGIATPEQAGEVAATADGVVVGSAIVKLFE
KHRGEELKGALATFVSSLKQAVDRGRYDN
>Mature_268_residues
SRITGTFAELKRTGKKALVTFITAGDPDLATTEELIPLLAENGADIIELGVPFSDPMADGPTIQLSSERALAAGTTLSRI
LATVKSVRGRTQVPIVLMGYFNPIFSYGLERFAADAAAAGVDGVLLVDLPPEEAGEFKACADRHGIDVIFLLTPTSDETR
IRTVTNRARGFIYYVSVTGVTGVRSGIEASVAGNVNIIKECSKVPVAVGFGIATPEQAGEVAATADGVVVGSAIVKLFEK
HRGEELKGALATFVSSLKQAVDRGRYDN

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=265, Percent_Identity=38.1132075471698, Blast_Score=180, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6321412, Length=238, Percent_Identity=44.9579831932773, Blast_Score=205, Evalue=8e-54,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_GEOMG (Q39SS2)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385427.1
- HSSP:   P00929
- ProteinModelPortal:   Q39SS2
- SMR:   Q39SS2
- STRING:   Q39SS2
- GeneID:   3740153
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2477
- NMPDR:   fig|269799.3.peg.2645
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q39SS2
- ProtClustDB:   PRK13111
- BioCyc:   GMET269799:GMET_2477-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28301; Mature: 28169

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRITGTFAELKRTGKKALVTFITAGDPDLATTEELIPLLAENGADIIELGVPFSDPMAD
CCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCC
GPTIQLSSERALAAGTTLSRILATVKSVRGRTQVPIVLMGYFNPIFSYGLERFAADAAAA
CCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC
GVDGVLLVDLPPEEAGEFKACADRHGIDVIFLLTPTSDETRIRTVTNRARGFIYYVSVTG
CCCCEEEEECCCCCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEEECC
VTGVRSGIEASVAGNVNIIKECSKVPVAVGFGIATPEQAGEVAATADGVVVGSAIVKLFE
CHHHHCCCCEECCCCHHHHHHHHCCCEEEECCCCCCHHCCCEEECCCCCHHHHHHHHHHH
KHRGEELKGALATFVSSLKQAVDRGRYDN
HHCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRITGTFAELKRTGKKALVTFITAGDPDLATTEELIPLLAENGADIIELGVPFSDPMAD
CCCHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCC
GPTIQLSSERALAAGTTLSRILATVKSVRGRTQVPIVLMGYFNPIFSYGLERFAADAAAA
CCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC
GVDGVLLVDLPPEEAGEFKACADRHGIDVIFLLTPTSDETRIRTVTNRARGFIYYVSVTG
CCCCEEEEECCCCCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEEEEECC
VTGVRSGIEASVAGNVNIIKECSKVPVAVGFGIATPEQAGEVAATADGVVVGSAIVKLFE
CHHHHCCCCEECCCCHHHHHHHHCCCEEEECCCCCCHHCCCEEECCCCCHHHHHHHHHHH
KHRGEELKGALATFVSSLKQAVDRGRYDN
HHCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA