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The map label for this gene is 78223649

Identifier: 78223649

GI number: 78223649

Start: 2770019

End: 2771572

Strand: Reverse

Name: 78223649

Synonym: Gmet_2446

Alternate gene names: NA

Gene position: 2771572-2770019 (Counterclockwise)

Preceding gene: 78223650

Following gene: 78223648

Centisome position: 69.33

GC content: 61.9

Gene sequence:

>1554_bases
ATGAACATTAACCGTCGTGATTTTCTGAAACTAGCCGGTGCAGGGGGGCTTGCCCTCGCCCTGCCCGGCTGCGCGGGGAA
TCTGCCTCTACCAAAGGAGGTCTTTCCCGACTTTGGCGACTCTGGGCGCCCCTGGCTCGGCCTTGCCACGTCGCTTCGCG
AGGAGCACGACTACGAGGCGCGCATCGAGGGGACGATTCCCTCCGCCCTCAGGGGGACTCTCTACCGCAACGGCCCGGCG
CTTTTCGACCGAGGCGGGCTGCGCAAGCGGACTTTGCTGGACGGGGATGGAATGGTGCAGTCCTTCACAGTCGGCGACGG
CGGGGTCCGCTACCGCAACCGCTACGTCCGGACGGAGAAATTCGTGGCCGAAGAGGCCGCCGGCCGTTTTCTGTATCCCA
CGTGGAGCACCCAGAAGCCGGGAGGTTTCTGGGGCAATTTCTGGCAGGCGGGGTCGGTTCCGAACCAGGCGGGCATAACA
GTTTTCTGGTGGCGCGGAAAACTCTACGCCTTCGACGAGGGGAACATTCCGTACGAGCTCGACCCGGAGTCCCTGGCCAC
CGCCGGTCCTTCGAATCTGGGGCTGACCCCCGGCACTGCCTTCTATTCGGCCCATCCCAAGCTTGATCCCCACACCGGGG
AATGGCTCCACTTCGGGGTGAAGTACGGCCCGAGGCCGCTCCTCCATCTCTCTGCCTTCGCGGCCGACGGAAGTCTGAAG
CGACACCGGACGTTTCCTCTCCCCCGCTATGTGTACATGCATGACTGGATCGTTTCCGGCCGGTACCTTATCCTCTCCCT
TCACCCTGTCGAAATCGAATTCTGGGGATTTCTCCTGGGATTTCGAAGCCTTTTTGCCTCGCTTCGCTGGCGGCCGGAAT
GGGGGAACCTGCTGATGATCGTCGAACGGGAAGGGGAGGGGAAGCCGCGCCTGGTGGAGACCGAGGCGTGTTACATGTGG
CATTCCTTCAACGCCTATGAGGAGGGAGGCGAAACCGTTGCCGACTTCATCGGCTACCGCAATCCGGACCACTTCGTGGG
AGATGATCCGGTCATCTCCGCCGTCATGGAAGGGCGTAGGGGACACTACGATTTTCCCGGAGAGATCATGCGGTACCGGA
TCGACCCCGTCCGTTGCACGGTTCGTCGGGAGACCCTGAACCCGGGGAGCTGCGAGTGGCCACGCATCGACGAACGCCTC
CGCTGCCGTCGCCATCGCATCGGCTACGCGGTGAAGTGCCTTCCCGGCGAGTTCTTCTGGTCCATTGTCATGCGCGTCGA
TTTCCTGAGCGGAACCACCGCCGAATATTCTTTCGGACGGGGGAAATACTGCACCGAAGCGGTCTTCGTGCCCAAACCGG
GAACATCTGCGGACGCAAGCCAATCGGCCGGAGAGGGGTGGCTCCTGACGGAGGTCTACGACAGCCATACCCGAAAAAGC
TTTCTTGCCATCTTGGACGCGGGAAGGGTTGCCGACGGCCCCCTTGCCATTGCCCATCTTACCCATCACGTTCCTTTCAG
CTACCATGGTTGGTGGCATCCTTCACCCGTTTAA

Upstream 100 bases:

>100_bases
ATGGGGCAAGATGTTCCATATGAGGTGATAGTAGCAATAAACTAACCATCGTTCCATTGATACTGATGGGAATAAATCTC
GTCTATTTCATGATTCTGCC

Downstream 100 bases:

>100_bases
TCATCAGGGGATATTAAAGGAGTTCGCGACGGTTCCGGAATTTACATGCAGAAAGAGATTGACAGAATGCGTGTCCGTGT
CCTATATTGAAAATCACTTT

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MNINRRDFLKLAGAGGLALALPGCAGNLPLPKEVFPDFGDSGRPWLGLATSLREEHDYEARIEGTIPSALRGTLYRNGPA
LFDRGGLRKRTLLDGDGMVQSFTVGDGGVRYRNRYVRTEKFVAEEAAGRFLYPTWSTQKPGGFWGNFWQAGSVPNQAGIT
VFWWRGKLYAFDEGNIPYELDPESLATAGPSNLGLTPGTAFYSAHPKLDPHTGEWLHFGVKYGPRPLLHLSAFAADGSLK
RHRTFPLPRYVYMHDWIVSGRYLILSLHPVEIEFWGFLLGFRSLFASLRWRPEWGNLLMIVEREGEGKPRLVETEACYMW
HSFNAYEEGGETVADFIGYRNPDHFVGDDPVISAVMEGRRGHYDFPGEIMRYRIDPVRCTVRRETLNPGSCEWPRIDERL
RCRRHRIGYAVKCLPGEFFWSIVMRVDFLSGTTAEYSFGRGKYCTEAVFVPKPGTSADASQSAGEGWLLTEVYDSHTRKS
FLAILDAGRVADGPLAIAHLTHHVPFSYHGWWHPSPV

Sequences:

>Translated_517_residues
MNINRRDFLKLAGAGGLALALPGCAGNLPLPKEVFPDFGDSGRPWLGLATSLREEHDYEARIEGTIPSALRGTLYRNGPA
LFDRGGLRKRTLLDGDGMVQSFTVGDGGVRYRNRYVRTEKFVAEEAAGRFLYPTWSTQKPGGFWGNFWQAGSVPNQAGIT
VFWWRGKLYAFDEGNIPYELDPESLATAGPSNLGLTPGTAFYSAHPKLDPHTGEWLHFGVKYGPRPLLHLSAFAADGSLK
RHRTFPLPRYVYMHDWIVSGRYLILSLHPVEIEFWGFLLGFRSLFASLRWRPEWGNLLMIVEREGEGKPRLVETEACYMW
HSFNAYEEGGETVADFIGYRNPDHFVGDDPVISAVMEGRRGHYDFPGEIMRYRIDPVRCTVRRETLNPGSCEWPRIDERL
RCRRHRIGYAVKCLPGEFFWSIVMRVDFLSGTTAEYSFGRGKYCTEAVFVPKPGTSADASQSAGEGWLLTEVYDSHTRKS
FLAILDAGRVADGPLAIAHLTHHVPFSYHGWWHPSPV
>Mature_517_residues
MNINRRDFLKLAGAGGLALALPGCAGNLPLPKEVFPDFGDSGRPWLGLATSLREEHDYEARIEGTIPSALRGTLYRNGPA
LFDRGGLRKRTLLDGDGMVQSFTVGDGGVRYRNRYVRTEKFVAEEAAGRFLYPTWSTQKPGGFWGNFWQAGSVPNQAGIT
VFWWRGKLYAFDEGNIPYELDPESLATAGPSNLGLTPGTAFYSAHPKLDPHTGEWLHFGVKYGPRPLLHLSAFAADGSLK
RHRTFPLPRYVYMHDWIVSGRYLILSLHPVEIEFWGFLLGFRSLFASLRWRPEWGNLLMIVEREGEGKPRLVETEACYMW
HSFNAYEEGGETVADFIGYRNPDHFVGDDPVISAVMEGRRGHYDFPGEIMRYRIDPVRCTVRRETLNPGSCEWPRIDERL
RCRRHRIGYAVKCLPGEFFWSIVMRVDFLSGTTAEYSFGRGKYCTEAVFVPKPGTSADASQSAGEGWLLTEVYDSHTRKS
FLAILDAGRVADGPLAIAHLTHHVPFSYHGWWHPSPV

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI74027270, Length=522, Percent_Identity=23.5632183908046, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32564756, Length=328, Percent_Identity=23.1707317073171, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24646669, Length=350, Percent_Identity=22.8571428571429, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 58287; Mature: 58287

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINRRDFLKLAGAGGLALALPGCAGNLPLPKEVFPDFGDSGRPWLGLATSLREEHDYEA
CCCCHHHHHEECCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCEE
RIEGTIPSALRGTLYRNGPALFDRGGLRKRTLLDGDGMVQSFTVGDGGVRYRNRYVRTEK
EEECCCCHHHHHHEECCCCCEECCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHH
FVAEEAAGRFLYPTWSTQKPGGFWGNFWQAGSVPNQAGITVFWWRGKLYAFDEGNIPYEL
HHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEE
DPESLATAGPSNLGLTPGTAFYSAHPKLDPHTGEWLHFGVKYGPRPLLHLSAFAADGSLK
CHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCC
RHRTFPLPRYVYMHDWIVSGRYLILSLHPVEIEFWGFLLGFRSLFASLRWRPEWGNLLMI
CCCCCCCCEEEEEEEEEECCEEEEEEEECEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VEREGEGKPRLVETEACYMWHSFNAYEEGGETVADFIGYRNPDHFVGDDPVISAVMEGRR
EEECCCCCCCEEECCEEEEEECCCCHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCC
GHYDFPGEIMRYRIDPVRCTVRRETLNPGSCEWPRIDERLRCRRHRIGYAVKCLPGEFFW
CCCCCCHHHEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHH
SIVMRVDFLSGTTAEYSFGRGKYCTEAVFVPKPGTSADASQSAGEGWLLTEVYDSHTRKS
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
FLAILDAGRVADGPLAIAHLTHHVPFSYHGWWHPSPV
HHHHHCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNINRRDFLKLAGAGGLALALPGCAGNLPLPKEVFPDFGDSGRPWLGLATSLREEHDYEA
CCCCHHHHHEECCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCEE
RIEGTIPSALRGTLYRNGPALFDRGGLRKRTLLDGDGMVQSFTVGDGGVRYRNRYVRTEK
EEECCCCHHHHHHEECCCCCEECCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHH
FVAEEAAGRFLYPTWSTQKPGGFWGNFWQAGSVPNQAGITVFWWRGKLYAFDEGNIPYEL
HHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEE
DPESLATAGPSNLGLTPGTAFYSAHPKLDPHTGEWLHFGVKYGPRPLLHLSAFAADGSLK
CHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCC
RHRTFPLPRYVYMHDWIVSGRYLILSLHPVEIEFWGFLLGFRSLFASLRWRPEWGNLLMI
CCCCCCCCEEEEEEEEEECCEEEEEEEECEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEE
VEREGEGKPRLVETEACYMWHSFNAYEEGGETVADFIGYRNPDHFVGDDPVISAVMEGRR
EEECCCCCCCEEECCEEEEEECCCCHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCC
GHYDFPGEIMRYRIDPVRCTVRRETLNPGSCEWPRIDERLRCRRHRIGYAVKCLPGEFFW
CCCCCCHHHEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHH
SIVMRVDFLSGTTAEYSFGRGKYCTEAVFVPKPGTSADASQSAGEGWLLTEVYDSHTRKS
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
FLAILDAGRVADGPLAIAHLTHHVPFSYHGWWHPSPV
HHHHHCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]