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The map label for this gene is 78223619

Identifier: 78223619

GI number: 78223619

Start: 2729096

End: 2730523

Strand: Reverse

Name: 78223619

Synonym: Gmet_2416

Alternate gene names: NA

Gene position: 2730523-2729096 (Counterclockwise)

Preceding gene: 78223620

Following gene: 78223618

Centisome position: 68.31

GC content: 61.97

Gene sequence:

>1428_bases
ATGAAGCCTCTGTTTCTCAATCCACCCACCTACGAAGATTTCGACGGCGGAGCCGGCGCCCGCTACCAGGCATCCCGGGA
GGTGACCTCCTTCTGGTACCCCACGTGGCTCTGCTTCCCCGCCGGGATGATCGACGGCTCCCGGGTGGTGGACGCGCCGG
TGCAACGTTTCACCATCGACGACTGCCTGGCCATTGCCCGTGATTTCGACATGGTGGTGATGTACACCTCCACGCCGACC
CTGGCCCTCGACATCGCCACCGCCCGCCGCGTCAAGGAGCAGAAGCCGGCCACGGTTACGGTCCTCACGGGCCCCCACGT
TTCCGTTCTCCCCGAGGAGACGCTGCGCCAGGGCGAAGGGGTCATCGACATCGTCTGCCGCGGCGAATTCGATTACACCA
CCAAGGAACTCTGCGAGGGCCGCGAGTGGGCAAAAGTGGACGGGATCAGCTTCTGGAAGGACGGGAAGATCCACCACACC
CCGGACCGCCCGCCGATCCAAGACCTGGACGCGCTCCCCTTCGTGGCTCCGGTTTACAAGCGGGATCTTCCCATTGCGGA
ATACGTCATACCCCACTTCAAGAACCCCTACGTCTCCATCTACTCCAGCCGTGGCTGCCCCTCCAAGTGCATCTACTGCC
TCTGGCCCCAGACCTTCTCGGGCCGGGCCATGCGGACCCGCTCGCCCCAGAACGTCTACGAAGAGGTGAAGTGGATCGTC
GACAACATCCCCGAGATGCGGGAGCTCTCCTTTGACGACGACACCTTCACCGCCGACCGCAAACACGCCCGCGAAGTGGC
GGCCAAGCTGAAGCCCCTGGGGATTTCCTGGACCATCAATGCCCGGGCAAACTGCGACTACGAAACCCTCAAGATCATGC
GCGAGGCAGGTCTCCGCCACGTGGTGGTGGGGTACGAGACCGGCAACGAGCAGATCCTGAAGAACATCAAGAAGGGGGTC
ACCAAGGAGCAGGCCATCGAGTTCACGAGAAATTGCAAGAAGCTCGGCCTTTCCGTCCACGGCGCCTTCATCATGGGGCT
CCCTGGCGAGACCCGCGACACCATCCGCGAGACCATCGAGTACGCCAAGGCCCTGGACCTCAACTCCATCCAGGCGTCAC
TGGCCTCGCCGTACCCCGGCACCGAGTTCTGGGATCTCTGCCGGCAGGAAGGGTGGATCGCCTCCGAGGCCTATATCGAC
GACACCGGCCACCAGATGTGCGTCATCAACTATCCTCACCTCTCCAACAAGGAGATCTTCGACGCGGTGGAGCTCTTCTA
CAACAAGTTCTACTTCCGTCCCAAGTACATCGGCCGCAGCATCATGAAGATGATCGTGGACGGCGAGGAGCGGAAGAAGC
TCCTGAAGGAAGGGAAGCAGTACCTGGAGTACATGCGGAAGCGGAAAGCTGCCGGATCATCCTGCTGA

Upstream 100 bases:

>100_bases
ACCCCTCCATCCTTGACTTTCCGTCGGCATCCCTTCATACTGCCGGTTTGTTCATGGGGCAAGCCCCCGTGAAGGTATCC
GTTTTTTCGGGAGATTTGCC

Downstream 100 bases:

>100_bases
CGGCTGCCATCCCATGAAAGAACTCATCATCAATGCCGACGACTTCGGCCTCTCCGACGGCGTCAACAAAGCGGTCATCA
AGGCCTGGCAGGAGGGTATC

Product: radical SAM family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKPLFLNPPTYEDFDGGAGARYQASREVTSFWYPTWLCFPAGMIDGSRVVDAPVQRFTIDDCLAIARDFDMVVMYTSTPT
LALDIATARRVKEQKPATVTVLTGPHVSVLPEETLRQGEGVIDIVCRGEFDYTTKELCEGREWAKVDGISFWKDGKIHHT
PDRPPIQDLDALPFVAPVYKRDLPIAEYVIPHFKNPYVSIYSSRGCPSKCIYCLWPQTFSGRAMRTRSPQNVYEEVKWIV
DNIPEMRELSFDDDTFTADRKHAREVAAKLKPLGISWTINARANCDYETLKIMREAGLRHVVVGYETGNEQILKNIKKGV
TKEQAIEFTRNCKKLGLSVHGAFIMGLPGETRDTIRETIEYAKALDLNSIQASLASPYPGTEFWDLCRQEGWIASEAYID
DTGHQMCVINYPHLSNKEIFDAVELFYNKFYFRPKYIGRSIMKMIVDGEERKKLLKEGKQYLEYMRKRKAAGSSC

Sequences:

>Translated_475_residues
MKPLFLNPPTYEDFDGGAGARYQASREVTSFWYPTWLCFPAGMIDGSRVVDAPVQRFTIDDCLAIARDFDMVVMYTSTPT
LALDIATARRVKEQKPATVTVLTGPHVSVLPEETLRQGEGVIDIVCRGEFDYTTKELCEGREWAKVDGISFWKDGKIHHT
PDRPPIQDLDALPFVAPVYKRDLPIAEYVIPHFKNPYVSIYSSRGCPSKCIYCLWPQTFSGRAMRTRSPQNVYEEVKWIV
DNIPEMRELSFDDDTFTADRKHAREVAAKLKPLGISWTINARANCDYETLKIMREAGLRHVVVGYETGNEQILKNIKKGV
TKEQAIEFTRNCKKLGLSVHGAFIMGLPGETRDTIRETIEYAKALDLNSIQASLASPYPGTEFWDLCRQEGWIASEAYID
DTGHQMCVINYPHLSNKEIFDAVELFYNKFYFRPKYIGRSIMKMIVDGEERKKLLKEGKQYLEYMRKRKAAGSSC
>Mature_475_residues
MKPLFLNPPTYEDFDGGAGARYQASREVTSFWYPTWLCFPAGMIDGSRVVDAPVQRFTIDDCLAIARDFDMVVMYTSTPT
LALDIATARRVKEQKPATVTVLTGPHVSVLPEETLRQGEGVIDIVCRGEFDYTTKELCEGREWAKVDGISFWKDGKIHHT
PDRPPIQDLDALPFVAPVYKRDLPIAEYVIPHFKNPYVSIYSSRGCPSKCIYCLWPQTFSGRAMRTRSPQNVYEEVKWIV
DNIPEMRELSFDDDTFTADRKHAREVAAKLKPLGISWTINARANCDYETLKIMREAGLRHVVVGYETGNEQILKNIKKGV
TKEQAIEFTRNCKKLGLSVHGAFIMGLPGETRDTIRETIEYAKALDLNSIQASLASPYPGTEFWDLCRQEGWIASEAYID
DTGHQMCVINYPHLSNKEIFDAVELFYNKFYFRPKYIGRSIMKMIVDGEERKKLLKEGKQYLEYMRKRKAAGSSC

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 54208; Mature: 54208

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLFLNPPTYEDFDGGAGARYQASREVTSFWYPTWLCFPAGMIDGSRVVDAPVQRFTID
CCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHCHHHHHHCCCCCCCCHHHCCHHHHHHHH
DCLAIARDFDMVVMYTSTPTLALDIATARRVKEQKPATVTVLTGPHVSVLPEETLRQGEG
HHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCEECCHHHHHCCCC
VIDIVCRGEFDYTTKELCEGREWAKVDGISFWKDGKIHHTPDRPPIQDLDALPFVAPVYK
EEEEEECCCCCCCHHHHHCCCCEEEECCEEECCCCCEECCCCCCCCCCCCCCCCHHHHHH
RDLPIAEYVIPHFKNPYVSIYSSRGCPSKCIYCLWPQTFSGRAMRTRSPQNVYEEVKWIV
CCCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCCCCCEECCCCHHHHHHHHHHHH
DNIPEMRELSFDDDTFTADRKHAREVAAKLKPLGISWTINARANCDYETLKIMREAGLRH
HCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEE
VVVGYETGNEQILKNIKKGVTKEQAIEFTRNCKKLGLSVHGAFIMGLPGETRDTIRETIE
EEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECEEEEECCCCHHHHHHHHHH
YAKALDLNSIQASLASPYPGTEFWDLCRQEGWIASEAYIDDTGHQMCVINYPHLSNKEIF
HHHHCCHHHHHHHHCCCCCCCHHHHHHHHCCCEECCCEECCCCCEEEEEECCCCCCHHHH
DAVELFYNKFYFRPKYIGRSIMKMIVDGEERKKLLKEGKQYLEYMRKRKAAGSSC
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKPLFLNPPTYEDFDGGAGARYQASREVTSFWYPTWLCFPAGMIDGSRVVDAPVQRFTID
CCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHCHHHHHHCCCCCCCCHHHCCHHHHHHHH
DCLAIARDFDMVVMYTSTPTLALDIATARRVKEQKPATVTVLTGPHVSVLPEETLRQGEG
HHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCEECCHHHHHCCCC
VIDIVCRGEFDYTTKELCEGREWAKVDGISFWKDGKIHHTPDRPPIQDLDALPFVAPVYK
EEEEEECCCCCCCHHHHHCCCCEEEECCEEECCCCCEECCCCCCCCCCCCCCCCHHHHHH
RDLPIAEYVIPHFKNPYVSIYSSRGCPSKCIYCLWPQTFSGRAMRTRSPQNVYEEVKWIV
CCCCHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCCCCCEECCCCHHHHHHHHHHHH
DNIPEMRELSFDDDTFTADRKHAREVAAKLKPLGISWTINARANCDYETLKIMREAGLRH
HCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEE
VVVGYETGNEQILKNIKKGVTKEQAIEFTRNCKKLGLSVHGAFIMGLPGETRDTIRETIE
EEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECEEEEECCCCHHHHHHHHHH
YAKALDLNSIQASLASPYPGTEFWDLCRQEGWIASEAYIDDTGHQMCVINYPHLSNKEIF
HHHHCCHHHHHHHHCCCCCCCHHHHHHHHCCCEECCCEECCCCCEEEEEECCCCCCHHHH
DAVELFYNKFYFRPKYIGRSIMKMIVDGEERKKLLKEGKQYLEYMRKRKAAGSSC
HHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]