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The map label for this gene is eno

Identifier: 78223575

GI number: 78223575

Start: 2681086

End: 2682375

Strand: Direct

Name: eno

Synonym: Gmet_2372

Alternate gene names: 78223575

Gene position: 2681086-2682375 (Clockwise)

Preceding gene: 78223526

Following gene: 78223576

Centisome position: 67.07

GC content: 60.85

Gene sequence:

>1290_bases
ATGAGCGAAATTACCGATGTCTACGCGCGAGAGATACTCGACTCCCGCGGCAACCCGACCCTGGAGGTCGAAGTTTTCCT
GGAGTCCGGCGTTATGGGAAGGGCTGCCGTTCCCTCCGGCGCATCTACCGGCGAGCGCGAGGCCCTGGAGCTTCGCGACG
GAGACAAGTCCCGCTACCTCGGCAAGGGGGTCCTCAAGGCAGTCGACAACGTCAACAACATCATTGCCGAGCAGATCATC
GGCATGGAAGCCACCGACCAGATCGGAATCGACCAGCGGATGCTGGATCTGGACGGCACCGAGTACAAGAGCAACCTGGG
AGCCAACGCCATTCTCGGCGTGTCCCTGGCAGTGGCCAAGGCCGCAGCCGAAGAAGTGGGGCTCCCCCTCTATCAGTACA
TCGGCGGCAGCAACGCCAAGGAGCTTCCGCTCCCCATGATGAACATCCTGAACGGCGGCGCCCATGCCGACAACAACGTC
GACATCCAGGAATTCATGATCATGCCGGCCGGCGCAAAATCCTTTGCCGAGGCGTTGCGCATGGGGGCTGAGATCTTCCA
TGCCCTGAAGGGTGTCCTGAAAGGTAAAGGATACAATACCGCCGTTGGTGACGAAGGCGGTTTCGCCCCTAACCTGAAGT
CCAACGAAGAGGCCCTGGAAGTCATCATGGACGCGATCCAGAAGGCCGGCTACAAGCCGGGCGAGGAAGTGCTCCTGGCC
CTGGACGTGGCCTCTTCGGAACTCTTCAACGATGGCGTCTACACCCTGGAGAACGAGGCCGAGCCCAAGAAAACCCCGGC
CCAGATGGTCGATTTCTACGAAAACCTCGTAAACAAATACCCGATCGTCTCCATCGAGGACGGCATGGCCGAGAACGACT
GGGACGGCTGGAAGCTCCTCACCGACCGCCTGGGCAAACGAATCCAGATCGTCGGCGATGACCTCTTCGTCACCAACCCC
CGTATCCTCAAAGAGGGGATCCAGAAGGGGATCGCCAACTCGATCCTGATCAAACTGAACCAGATCGGCTCCCTCACCGA
AACCCTGGACGCCATCGAAATGGCCAAGCGCGCCGGTTACACCTGCGTCATCTCCCACCGCTCGGGCGAGACCGAGGACA
CCACCCTGGCCGACCTCTCCGTGGCGGTCAACGCCGGCCAGATCAAGACCGGTTCCCTCTGCCGCACCGACCGGGTCGCC
AAGTACAACCAGCTCCTGCGGATCGAGGACGAGCTCGACACCACCGCCCTCTTCCGGGGCAAGGATGTCTTCTACAACAT
CCGCAAGTAG

Upstream 100 bases:

>100_bases
ACATTAAAAATGTTTCCTTTCAGCATCAATTCTGATATATATTCCGCTGTTTTGCCTATGGTCGGAAGACCAAAGTACAT
ATTTCTGCAAGGGGGCCAGA

Downstream 100 bases:

>100_bases
TCGGGGCGGAACACCTTAGCAACACGATGAAAGGCGGGAGCGATCCCGCCTTTCATTCGTTTGGGCACCTCGCGCCCCTG
AACCGCCAGCCAGTGCGAAT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDKSRYLGKGVLKAVDNVNNIIAEQII
GMEATDQIGIDQRMLDLDGTEYKSNLGANAILGVSLAVAKAAAEEVGLPLYQYIGGSNAKELPLPMMNILNGGAHADNNV
DIQEFMIMPAGAKSFAEALRMGAEIFHALKGVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMDAIQKAGYKPGEEVLLA
LDVASSELFNDGVYTLENEAEPKKTPAQMVDFYENLVNKYPIVSIEDGMAENDWDGWKLLTDRLGKRIQIVGDDLFVTNP
RILKEGIQKGIANSILIKLNQIGSLTETLDAIEMAKRAGYTCVISHRSGETEDTTLADLSVAVNAGQIKTGSLCRTDRVA
KYNQLLRIEDELDTTALFRGKDVFYNIRK

Sequences:

>Translated_429_residues
MSEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDKSRYLGKGVLKAVDNVNNIIAEQII
GMEATDQIGIDQRMLDLDGTEYKSNLGANAILGVSLAVAKAAAEEVGLPLYQYIGGSNAKELPLPMMNILNGGAHADNNV
DIQEFMIMPAGAKSFAEALRMGAEIFHALKGVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMDAIQKAGYKPGEEVLLA
LDVASSELFNDGVYTLENEAEPKKTPAQMVDFYENLVNKYPIVSIEDGMAENDWDGWKLLTDRLGKRIQIVGDDLFVTNP
RILKEGIQKGIANSILIKLNQIGSLTETLDAIEMAKRAGYTCVISHRSGETEDTTLADLSVAVNAGQIKTGSLCRTDRVA
KYNQLLRIEDELDTTALFRGKDVFYNIRK
>Mature_428_residues
SEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDKSRYLGKGVLKAVDNVNNIIAEQIIG
MEATDQIGIDQRMLDLDGTEYKSNLGANAILGVSLAVAKAAAEEVGLPLYQYIGGSNAKELPLPMMNILNGGAHADNNVD
IQEFMIMPAGAKSFAEALRMGAEIFHALKGVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMDAIQKAGYKPGEEVLLAL
DVASSELFNDGVYTLENEAEPKKTPAQMVDFYENLVNKYPIVSIEDGMAENDWDGWKLLTDRLGKRIQIVGDDLFVTNPR
ILKEGIQKGIANSILIKLNQIGSLTETLDAIEMAKRAGYTCVISHRSGETEDTTLADLSVAVNAGQIKTGSLCRTDRVAK
YNQLLRIEDELDTTALFRGKDVFYNIRK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=53.5962877030162, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=51.9721577726218, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI301897477, Length=427, Percent_Identity=52.4590163934426, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=427, Percent_Identity=52.4590163934426, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=425, Percent_Identity=48, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=352, Percent_Identity=26.9886363636364, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI169201757, Length=352, Percent_Identity=26.9886363636364, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI239744207, Length=352, Percent_Identity=26.9886363636364, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=64.7058823529412, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.0092592592593, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=53.0092592592593, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=44.2708333333333, Blast_Score=181, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=436, Percent_Identity=50.9174311926605, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.8845265588915, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.8845265588915, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.6535796766744, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321968, Length=436, Percent_Identity=50.2293577981651, Blast_Score=381, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=52.093023255814, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=52.093023255814, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=52.093023255814, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=52.093023255814, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=52.093023255814, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=52.093023255814, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_GEOMG (Q39T27)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385322.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q39T27
- SMR:   Q39T27
- STRING:   Q39T27
- GeneID:   3740049
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2372
- NMPDR:   fig|269799.3.peg.2813
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q39T27
- ProtClustDB:   PRK00077
- BioCyc:   GMET269799:GMET_2372-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46543; Mature: 46412

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 338-338 BINDING 155-155 BINDING 164-164 BINDING 286-286 BINDING 313-313 BINDING 338-338 BINDING 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHH
GKGVLKAVDNVNNIIAEQIIGMEATDQIGIDQRMLDLDGTEYKSNLGANAILGVSLAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCCHHHHCCCCCHHHHHHHHHHH
AAAEEVGLPLYQYIGGSNAKELPLPMMNILNGGAHADNNVDIQEFMIMPAGAKSFAEALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
MGAEIFHALKGVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMDAIQKAGYKPGEEVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEE
LDVASSELFNDGVYTLENEAEPKKTPAQMVDFYENLVNKYPIVSIEDGMAENDWDGWKLL
EEHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
TDRLGKRIQIVGDDLFVTNPRILKEGIQKGIANSILIKLNQIGSLTETLDAIEMAKRAGY
HHHCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHCCC
TCVISHRSGETEDTTLADLSVAVNAGQIKTGSLCRTDRVAKYNQLLRIEDELDTTALFRG
EEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHEEEHHHCCHHHHCCC
KDVFYNIRK
CCCEEEECC
>Mature Secondary Structure 
SEITDVYAREILDSRGNPTLEVEVFLESGVMGRAAVPSGASTGEREALELRDGDKSRYL
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHH
GKGVLKAVDNVNNIIAEQIIGMEATDQIGIDQRMLDLDGTEYKSNLGANAILGVSLAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCCHHHHCCCCCHHHHHHHHHHH
AAAEEVGLPLYQYIGGSNAKELPLPMMNILNGGAHADNNVDIQEFMIMPAGAKSFAEALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
MGAEIFHALKGVLKGKGYNTAVGDEGGFAPNLKSNEEALEVIMDAIQKAGYKPGEEVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEE
LDVASSELFNDGVYTLENEAEPKKTPAQMVDFYENLVNKYPIVSIEDGMAENDWDGWKLL
EEHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
TDRLGKRIQIVGDDLFVTNPRILKEGIQKGIANSILIKLNQIGSLTETLDAIEMAKRAGY
HHHCCCEEEEEECCEEECCHHHHHHHHHHHHHHHHEEEHHHHCCHHHHHHHHHHHHHCCC
TCVISHRSGETEDTTLADLSVAVNAGQIKTGSLCRTDRVAKYNQLLRIEDELDTTALFRG
EEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHEEEHHHCCHHHHCCC
KDVFYNIRK
CCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA