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The map label for this gene is waaA [H]

Identifier: 78223551

GI number: 78223551

Start: 2654504

End: 2655814

Strand: Reverse

Name: waaA [H]

Synonym: Gmet_2348

Alternate gene names: 78223551

Gene position: 2655814-2654504 (Counterclockwise)

Preceding gene: 78223552

Following gene: 78223550

Centisome position: 66.44

GC content: 64.68

Gene sequence:

>1311_bases
ATGATATTTCTGGTGTATGACATACTACTGCTCCTCCTCTCCCCCCTGATCCTTATCCACCACGCGTGGCGGACGGTGAG
CCGGGGGAGAAGTTTCGCCGGTTTCGGGGAGCGGTTCGGGTGCATAGCGCCGGAGAGCCTCGCACGGGTTGCCGGCAAGG
GGCCGATCTGGGTCCACGCGGTGTCGGTGGGGGAAACCATGGCGGTGAAGCCGCTCCTGCGGGAGCTGAAGCGCCGCTTT
CCCGAACGTCCCCTCGTGCTGTCATCGGTGACGGAGACGGGGCGGTCGGTGGCGGTCACTATCCCCGAAGCGGATCTGGT
GGTCTACTTCCCTTTTGATTTCGGCTTCGCCGTTGCCCGGGCGCTTCGACTCGTTGCGCCCTCGCTGGTCATCGTGGTGG
AAACCGAGATCTGGCCCAATTTCCTCCGCCACGCCCGCCGGACGGGGATCCCGTCAGTCATGGTCAACGGCCGCATCTCG
GACCGTTCCTTCCCTCGCTATCTCCGGTTCTCCCGCTTTTTTGCTCCCATCCTGAACAACCTGTCAGCCCTCTGCATGCA
GAGCAGCGAGGACGCCCGCCGCATAATCGCCGTCGGAGCGCCTGCGGAGCGTGTCCACGTAACGCGCAATCTCAAGTATG
ATCTGCCTGTCAGATCGCTCACGCCGGCTGAGCGCCAGGAACTGCTCTGCAGCTATCGCCTGCCTGCCGGCGCACTCATC
ATCACAGCGGGGAGCACCCATGCCGGGGAAGAGGAGGTTGTGGCGGACATCTATGCCCGTCTCGCGGGAGAGAGATCTGA
TCTCTTCCTGGTTCTTGTGCCGCGTCACCCGGAACGGGCCGTCGAGGTGGGAACCCTCCTGGAGGGGAAAGGGATTCCCT
TCGTGCGCCGTTCGGCCTTGGACAAGGTTCAGGAGCAGCCACGCGGCGGCGTTCTCCTAGTGGACACCATCGGCGAACTG
ATGAAGCTCTACGCCCTGTCGGACGTGGTGTTCGTGGGAGGGAGCCTTGTCCCGGTGGGGGGGCATAACCTCCTGGAGCC
GGCATCGGTGGGCGCGCCGGTCCTGTTCGGCCCCCACATGCACAATTTCCGCGAGATCACGGCCCTCGTGCTCGGTGCCG
GAGCGGGAGAGCAGGTCAACGATCATGGGGAGCTGGAAGCGACCCTGCGCCGTCTGTTGGCCGATGAACCCGCCCGCCGG
TCCATGGGGGAGAACGGCATCCGGCTCATGGCGGACCAGGGAGGCGCTGCCGCACGGCATCTGGAGATCATCGGCCCGCT
CATTGAGCGAGGTAGCGAGGTAGCGAGGTAG

Upstream 100 bases:

>100_bases
GGCGGCCTCTACCGCAAGCTCTACGATATGCAATTCAGTTAGAGGCCGGGACCGGGGACCAGGGACCAGGGACCGGCGGC
AGCGGGGATAGAGCGTTTCG

Downstream 100 bases:

>100_bases
CGAGGTTTTACCATTTTGCTATCTCACCCGGATGAAATATGACACTCGAAGGATATTTCCGAAACCTTGTTTCCGGCAAG
CGGCGGGGGATGCTGGATCG

Product: three-deoxy-D-manno-octulosonic-acid transferase-like

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MIFLVYDILLLLLSPLILIHHAWRTVSRGRSFAGFGERFGCIAPESLARVAGKGPIWVHAVSVGETMAVKPLLRELKRRF
PERPLVLSSVTETGRSVAVTIPEADLVVYFPFDFGFAVARALRLVAPSLVIVVETEIWPNFLRHARRTGIPSVMVNGRIS
DRSFPRYLRFSRFFAPILNNLSALCMQSSEDARRIIAVGAPAERVHVTRNLKYDLPVRSLTPAERQELLCSYRLPAGALI
ITAGSTHAGEEEVVADIYARLAGERSDLFLVLVPRHPERAVEVGTLLEGKGIPFVRRSALDKVQEQPRGGVLLVDTIGEL
MKLYALSDVVFVGGSLVPVGGHNLLEPASVGAPVLFGPHMHNFREITALVLGAGAGEQVNDHGELEATLRRLLADEPARR
SMGENGIRLMADQGGAAARHLEIIGPLIERGSEVAR

Sequences:

>Translated_436_residues
MIFLVYDILLLLLSPLILIHHAWRTVSRGRSFAGFGERFGCIAPESLARVAGKGPIWVHAVSVGETMAVKPLLRELKRRF
PERPLVLSSVTETGRSVAVTIPEADLVVYFPFDFGFAVARALRLVAPSLVIVVETEIWPNFLRHARRTGIPSVMVNGRIS
DRSFPRYLRFSRFFAPILNNLSALCMQSSEDARRIIAVGAPAERVHVTRNLKYDLPVRSLTPAERQELLCSYRLPAGALI
ITAGSTHAGEEEVVADIYARLAGERSDLFLVLVPRHPERAVEVGTLLEGKGIPFVRRSALDKVQEQPRGGVLLVDTIGEL
MKLYALSDVVFVGGSLVPVGGHNLLEPASVGAPVLFGPHMHNFREITALVLGAGAGEQVNDHGELEATLRRLLADEPARR
SMGENGIRLMADQGGAAARHLEIIGPLIERGSEVAR
>Mature_436_residues
MIFLVYDILLLLLSPLILIHHAWRTVSRGRSFAGFGERFGCIAPESLARVAGKGPIWVHAVSVGETMAVKPLLRELKRRF
PERPLVLSSVTETGRSVAVTIPEADLVVYFPFDFGFAVARALRLVAPSLVIVVETEIWPNFLRHARRTGIPSVMVNGRIS
DRSFPRYLRFSRFFAPILNNLSALCMQSSEDARRIIAVGAPAERVHVTRNLKYDLPVRSLTPAERQELLCSYRLPAGALI
ITAGSTHAGEEEVVADIYARLAGERSDLFLVLVPRHPERAVEVGTLLEGKGIPFVRRSALDKVQEQPRGGVLLVDTIGEL
MKLYALSDVVFVGGSLVPVGGHNLLEPASVGAPVLFGPHMHNFREITALVLGAGAGEQVNDHGELEATLRRLLADEPARR
SMGENGIRLMADQGGAAARHLEIIGPLIERGSEVAR

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers a single molecule of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=437, Percent_Identity=36.8421052631579, Blast_Score=241, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47672; Mature: 47672

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLVYDILLLLLSPLILIHHAWRTVSRGRSFAGFGERFGCIAPESLARVAGKGPIWVHA
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEE
VSVGETMAVKPLLRELKRRFPERPLVLSSVTETGRSVAVTIPEADLVVYFPFDFGFAVAR
EECCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCEEEEEECCCCEEEEECCCHHHHHHH
ALRLVAPSLVIVVETEIWPNFLRHARRTGIPSVMVNGRISDRSFPRYLRFSRFFAPILNN
HHHHHCCCEEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
LSALCMQSSEDARRIIAVGAPAERVHVTRNLKYDLPVRSLTPAERQELLCSYRLPAGALI
HHHHHHCCCCCCCEEEEECCCHHHEEEEECCEECCCCCCCCHHHHHHHHHHCCCCCCEEE
ITAGSTHAGEEEVVADIYARLAGERSDLFLVLVPRHPERAVEVGTLLEGKGIPFVRRSAL
EEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHH
DKVQEQPRGGVLLVDTIGELMKLYALSDVVFVGGSLVPVGGHNLLEPASVGAPVLFGPHM
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHCCCCEEECCCC
HNFREITALVLGAGAGEQVNDHGELEATLRRLLADEPARRSMGENGIRLMADQGGAAARH
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCHHHH
LEIIGPLIERGSEVAR
HHHHHHHHHCCHHHCC
>Mature Secondary Structure
MIFLVYDILLLLLSPLILIHHAWRTVSRGRSFAGFGERFGCIAPESLARVAGKGPIWVHA
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEE
VSVGETMAVKPLLRELKRRFPERPLVLSSVTETGRSVAVTIPEADLVVYFPFDFGFAVAR
EECCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCEEEEEECCCCEEEEECCCHHHHHHH
ALRLVAPSLVIVVETEIWPNFLRHARRTGIPSVMVNGRISDRSFPRYLRFSRFFAPILNN
HHHHHCCCEEEEEECHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
LSALCMQSSEDARRIIAVGAPAERVHVTRNLKYDLPVRSLTPAERQELLCSYRLPAGALI
HHHHHHCCCCCCCEEEEECCCHHHEEEEECCEECCCCCCCCHHHHHHHHHHCCCCCCEEE
ITAGSTHAGEEEVVADIYARLAGERSDLFLVLVPRHPERAVEVGTLLEGKGIPFVRRSAL
EEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHH
DKVQEQPRGGVLLVDTIGELMKLYALSDVVFVGGSLVPVGGHNLLEPASVGAPVLFGPHM
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHCCCCEEECCCC
HNFREITALVLGAGAGEQVNDHGELEATLRRLLADEPARRSMGENGIRLMADQGGAAARH
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCHHHH
LEIIGPLIERGSEVAR
HHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952982; 7542800 [H]