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The map label for this gene is gmk

Identifier: 78223530

GI number: 78223530

Start: 2634731

End: 2635342

Strand: Reverse

Name: gmk

Synonym: Gmet_2327

Alternate gene names: 78223530

Gene position: 2635342-2634731 (Counterclockwise)

Preceding gene: 78223531

Following gene: 78223529

Centisome position: 65.93

GC content: 56.54

Gene sequence:

>612_bases
ATGAAACGCGAAGGGGTTCTCTACATCATCTCGGCGCCGTCGGGGGCGGGTAAGACTACGCTCTGCAAGGAAATAATTGA
CATCTTTCCGCACCTCCGGCATTCTGTCAGCTATACGACGCGGCAGCCCCGAGCCGGTGAAGTGCATGGAAAAGACTATT
ATTTTATTTCCATGGACGAGTTCCGGAGCATGGTCGATAAGGGTGAGTTTGCCGAGTGGGCCGAGGTTCACGGCAACTGT
TACGGTACGTCCATTCGCACCCTTGAGGAGTGCCGCATCACGGGAATCGACCTGATTCTCGATATTGACATCCAGGGCGC
GCGTCAGCTCAAGGAGCGTTACGAGGGCGGCGTCTACATATTCATTCTCCCCCCCAGCTACGAGGAACTCCGTCGTCGGC
TCAACGGCCGTTCCTCCGACAGCGACGATGTCATTAGCCGGCGCATTGATGCCGCTGCCGGCGAGATTCGCGAATCCCGC
TGGTACGACTACATTATAGTCAACGATCAGTTCAGCCGGGCAGTAGAAGAACTCAAATCGGTCGTGGTTGCAGAGCGGTG
CCGGACGTTCCGGGTTCTTGAAACGGTGACCGAACGGTTCGACATGGGCTGA

Upstream 100 bases:

>100_bases
GAACATAGAATAGGGGCCACGGGCGGGGAACCAGGGACCAGGAAAGTGTCGTTGGATTTCACTGGTCCCCGGTCCCCGGT
CCCTGGTCCCCGGGGTTACC

Downstream 100 bases:

>100_bases
GACAGACCAGGAACCAACATCCCATAAGCACTGAGACACCGGCCGGCACAGATGCCGGTTTTTGAAAGGAGTAGCACAAT
GGCACGGGTAACAGTTGAAG

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKREGVLYIISAPSGAGKTTLCKEIIDIFPHLRHSVSYTTRQPRAGEVHGKDYYFISMDEFRSMVDKGEFAEWAEVHGNC
YGTSIRTLEECRITGIDLILDIDIQGARQLKERYEGGVYIFILPPSYEELRRRLNGRSSDSDDVISRRIDAAAGEIRESR
WYDYIIVNDQFSRAVEELKSVVVAERCRTFRVLETVTERFDMG

Sequences:

>Translated_203_residues
MKREGVLYIISAPSGAGKTTLCKEIIDIFPHLRHSVSYTTRQPRAGEVHGKDYYFISMDEFRSMVDKGEFAEWAEVHGNC
YGTSIRTLEECRITGIDLILDIDIQGARQLKERYEGGVYIFILPPSYEELRRRLNGRSSDSDDVISRRIDAAAGEIRESR
WYDYIIVNDQFSRAVEELKSVVVAERCRTFRVLETVTERFDMG
>Mature_203_residues
MKREGVLYIISAPSGAGKTTLCKEIIDIFPHLRHSVSYTTRQPRAGEVHGKDYYFISMDEFRSMVDKGEFAEWAEVHGNC
YGTSIRTLEECRITGIDLILDIDIQGARQLKERYEGGVYIFILPPSYEELRRRLNGRSSDSDDVISRRIDAAAGEIRESR
WYDYIIVNDQFSRAVEELKSVVVAERCRTFRVLETVTERFDMG

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874833, Length=184, Percent_Identity=36.9565217391304, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI4504221, Length=184, Percent_Identity=36.9565217391304, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI226874831, Length=184, Percent_Identity=36.9565217391304, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI21361598, Length=187, Percent_Identity=29.9465240641711, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI14780902, Length=191, Percent_Identity=25.6544502617801, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI38570142, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI111154074, Length=182, Percent_Identity=32.4175824175824, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4505237, Length=175, Percent_Identity=26.2857142857143, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI262118259, Length=175, Percent_Identity=26.2857142857143, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI262118263, Length=175, Percent_Identity=26.2857142857143, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI262118255, Length=175, Percent_Identity=26.2857142857143, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI52630447, Length=196, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI186700627, Length=181, Percent_Identity=23.2044198895028, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI186972120, Length=181, Percent_Identity=23.2044198895028, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI186700629, Length=181, Percent_Identity=23.2044198895028, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI21389483, Length=182, Percent_Identity=28.021978021978, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790080, Length=190, Percent_Identity=49.4736842105263, Blast_Score=193, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI193203132, Length=175, Percent_Identity=38.2857142857143, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17508835, Length=175, Percent_Identity=38.2857142857143, Blast_Score=133, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71988041, Length=183, Percent_Identity=23.4972677595628, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988048, Length=183, Percent_Identity=23.4972677595628, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320662, Length=182, Percent_Identity=44.5054945054945, Blast_Score=167, Evalue=8e-43,
Organism=Drosophila melanogaster, GI21356141, Length=187, Percent_Identity=37.9679144385027, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161077626, Length=175, Percent_Identity=26.8571428571429, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640542, Length=175, Percent_Identity=26.8571428571429, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45554489, Length=175, Percent_Identity=26.8571428571429, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI85724806, Length=175, Percent_Identity=26.8571428571429, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45554481, Length=175, Percent_Identity=26.8571428571429, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI116007136, Length=175, Percent_Identity=26.8571428571429, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI221330162, Length=197, Percent_Identity=28.4263959390863, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI221330166, Length=197, Percent_Identity=28.4263959390863, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI221330164, Length=197, Percent_Identity=28.4263959390863, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_GEOMG (Q39T72)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385277.1
- HSSP:   P60546
- ProteinModelPortal:   Q39T72
- SMR:   Q39T72
- STRING:   Q39T72
- GeneID:   3739488
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2327
- NMPDR:   fig|269799.3.peg.2571
- eggNOG:   COG0194
- HOGENOM:   HBG671982
- OMA:   EWAEFAG
- PhylomeDB:   Q39T72
- ProtClustDB:   PRK00300
- BioCyc:   GMET269799:GMET_2327-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 23404; Mature: 23404

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREGVLYIISAPSGAGKTTLCKEIIDIFPHLRHSVSYTTRQPRAGEVHGKDYYFISMDE
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHH
FRSMVDKGEFAEWAEVHGNCYGTSIRTLEECRITGIDLILDIDIQGARQLKERYEGGVYI
HHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCEEE
FILPPSYEELRRRLNGRSSDSDDVISRRIDAAAGEIRESRWYDYIIVNDQFSRAVEELKS
EEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
VVVAERCRTFRVLETVTERFDMG
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKREGVLYIISAPSGAGKTTLCKEIIDIFPHLRHSVSYTTRQPRAGEVHGKDYYFISMDE
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHH
FRSMVDKGEFAEWAEVHGNCYGTSIRTLEECRITGIDLILDIDIQGARQLKERYEGGVYI
HHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCEEE
FILPPSYEELRRRLNGRSSDSDDVISRRIDAAAGEIRESRWYDYIIVNDQFSRAVEELKS
EEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHH
VVVAERCRTFRVLETVTERFDMG
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA