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The map label for this gene is relA [H]

Identifier: 78223528

GI number: 78223528

Start: 2632233

End: 2634383

Strand: Reverse

Name: relA [H]

Synonym: Gmet_2325

Alternate gene names: 78223528

Gene position: 2634383-2632233 (Counterclockwise)

Preceding gene: 78223529

Following gene: 78223527

Centisome position: 65.9

GC content: 59.65

Gene sequence:

>2151_bases
ATGATCAGACTCAACGATATCCTCGATAAGGTTGGTTCATACAACCCCGCGCCGGACCTGGACCTGGTGCGCAAGGCCTA
CGTATACTGCGCCAAGGTCCACCAGGGGCAGACCCGTCTTTCGGGCGAGCCCTATCTGGTTCACCCCATGGAGGTGGCGG
GGCTTCTGGCCGACCTCCGGCTCGATGTCCCCACCGTGGTGACGGGGCTTCTCCATGACACCATCGAGGATACCCTGACC
ACCCGCGAGGAACTGGAAGGGCTGTTCGGTGCCGAGGTGGCCAACCTCGTGGACGGTGTCACCAAGATCAGCAAAATCCA
CTTCAAGACCAAGGAAGAGAGCCAGGCCGAGAACTTCCGCAAGATGCTCCTGGCCATGGCCAATGACATCCGGGTCATTC
TAGTGAAGCTGGCCGACCGCCTCCACAACATGCGGACCCTCCAGTACCAGCCCGAGCCGAAGCAGCGCTCCATCGCCCGC
GAAACCCTCGACATCTACGCACCTATCGCTAACCGTCTCGGTATTTCCTGGATCAAGAGCGAACTGGAGGATCTCTCCTT
CCGGTACCTGGAGCCACAGATCTACTACGATCTCGCCTCCAAGGTGACCAAGAAGAAGAAGGAGCGGGAGAGCTATGTCG
ATGAGGTGCGGAAGATCATCGTCGACAAGCTTGTGGAGCACGACATCAAGGGGGAGGTCTTCGGCCGGAGCAAGCATCTC
TACTCCATCTGGCGCAAGATGCAGAGCCGCAGTGTCGATATCGACCAGATCTACGACCTGATCGCCATTCGCGTCATGGT
GAACGATATCCGCGAGTGCTATGAGGTGCTGGGTCTCATCCATTCCACCTGGAAGCCGATCCCCGGCCGCTTCAAGGACT
ACATCGCCATGCCCAAGGGGAACATGTACCAGTCGCTCCACACGACGGTCATCGGTCCCCACGGGGAGCGGATGGAGGTG
CAGATCCGCACCTATGACATGCACGGGGTTGCGGAGGCGGGGATCGCGGCTCACTGGAAGTACAAGGAGGGGAAGGGGTA
CGACGAGAAGGAGGTGAAGCGCTTTGCCTGGCTGCGTCAGCTCCTTGAATGGCAGCAGGAGCTCCAGGACTCCCATGAGT
TCATGAGCACCGTCAAGGTGGAGCTTTTTCCCGAGGAGGTCTACGTCTTCACGCCGAACGGCGATGTGAAGAGCTTTCCC
AAGGGGTCGACCCCCATCGACTTCGCCTACACCATCCACACCGACCTGGGGCATCGCTGCGTCGGCGCCAAGGTGAACGG
CAAGCTGGTCCCCCTCAAGTACGAGCTCAAGAACGGCGATATCGTCGAGGTTATCACCTCTCCCCACCACACCCCCAGCA
AGGATTGGCTCAAGATCGTCAGGAGCTCCCGGGCGCGGAACAAGATCCGGGCCTGGATAAAGACCGAAGAGCGGGTCCGG
AGCATCTCCCTTGGCAAGGAGATCATGGAGAAGGAATTCCGACGTTTCTCCTTGAACCTGGGCAAGCTCCAGAAGACGGG
GGAGCTGAAGAGGGTGGCCACGGAATTCGGCTATGTAGGCGACGACGATCTCATGGCGGCCGTGGGATATGGAAAGCTCT
CCTGCAACCAGGTGCTCGGCAAACTGCTGCCCGAGGAAAAGCTCGCCGAGAAGCAGGAACTGAAGGAATCGCGGATCGGC
AAGGCCATCAGCAAGCTGACGGGAAAATCCACGAGCGCCATTCAGATAGGCGGCGTTGACGATGTGCTCGTTCGCTTCGG
CAAGTGCTGCAATCCGGTCCCCGGTGACGAAATCGTCGGGTTCATCACCAGGGGACGGGGTGTGACCGTCCACACCGCCG
ACTGTCCCATTGCCCTGGAGAACGATCCGGAGCGGCGCATCGCCGTTGACTGGAACCGGGAGCGGAAGGCGGCCCTGCCG
GTGAAGATCAAGGTCTCCTGTCACGACCAGAAGGGGATTCTGGCCAATATCACCCTGGCCATTACCAACTGTGAGGCGAA
CATATCGAGCGCCTCCATCCAGAGCACCGTGGACCGGCGAGGGGTGAACATTTTCGAGGTGGACGTCACGAGCCTTGAGC
ACCTGACCCGGGTCATGAACAACATCATGAAGGTAAGCGGCGTCACCCATGTGGAACGGATGAAGTCGTAG

Upstream 100 bases:

>100_bases
GCAAGGTAGGGTACGAGCTGACTTCGCGCAAGGCAAAGTAGTAAGCCTGGCTACAGGGGCAGAAGCGTAATGCTTCCGCC
CTTTTTTTGTCCTTTTTTCC

Downstream 100 bases:

>100_bases
GAAGGAGCATGCGATGAAAGAGATTATTGCCACCGACAAGGCACCCAAGGCGATCGGGCCCTATTCTCAGGCAGTGAAGG
CGGGGGGCTTCCTGTTCCTC

Product: (p)ppGpp synthetase I (GTP pyrophosphokinase), SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MIRLNDILDKVGSYNPAPDLDLVRKAYVYCAKVHQGQTRLSGEPYLVHPMEVAGLLADLRLDVPTVVTGLLHDTIEDTLT
TREELEGLFGAEVANLVDGVTKISKIHFKTKEESQAENFRKMLLAMANDIRVILVKLADRLHNMRTLQYQPEPKQRSIAR
ETLDIYAPIANRLGISWIKSELEDLSFRYLEPQIYYDLASKVTKKKKERESYVDEVRKIIVDKLVEHDIKGEVFGRSKHL
YSIWRKMQSRSVDIDQIYDLIAIRVMVNDIRECYEVLGLIHSTWKPIPGRFKDYIAMPKGNMYQSLHTTVIGPHGERMEV
QIRTYDMHGVAEAGIAAHWKYKEGKGYDEKEVKRFAWLRQLLEWQQELQDSHEFMSTVKVELFPEEVYVFTPNGDVKSFP
KGSTPIDFAYTIHTDLGHRCVGAKVNGKLVPLKYELKNGDIVEVITSPHHTPSKDWLKIVRSSRARNKIRAWIKTEERVR
SISLGKEIMEKEFRRFSLNLGKLQKTGELKRVATEFGYVGDDDLMAAVGYGKLSCNQVLGKLLPEEKLAEKQELKESRIG
KAISKLTGKSTSAIQIGGVDDVLVRFGKCCNPVPGDEIVGFITRGRGVTVHTADCPIALENDPERRIAVDWNRERKAALP
VKIKVSCHDQKGILANITLAITNCEANISSASIQSTVDRRGVNIFEVDVTSLEHLTRVMNNIMKVSGVTHVERMKS

Sequences:

>Translated_716_residues
MIRLNDILDKVGSYNPAPDLDLVRKAYVYCAKVHQGQTRLSGEPYLVHPMEVAGLLADLRLDVPTVVTGLLHDTIEDTLT
TREELEGLFGAEVANLVDGVTKISKIHFKTKEESQAENFRKMLLAMANDIRVILVKLADRLHNMRTLQYQPEPKQRSIAR
ETLDIYAPIANRLGISWIKSELEDLSFRYLEPQIYYDLASKVTKKKKERESYVDEVRKIIVDKLVEHDIKGEVFGRSKHL
YSIWRKMQSRSVDIDQIYDLIAIRVMVNDIRECYEVLGLIHSTWKPIPGRFKDYIAMPKGNMYQSLHTTVIGPHGERMEV
QIRTYDMHGVAEAGIAAHWKYKEGKGYDEKEVKRFAWLRQLLEWQQELQDSHEFMSTVKVELFPEEVYVFTPNGDVKSFP
KGSTPIDFAYTIHTDLGHRCVGAKVNGKLVPLKYELKNGDIVEVITSPHHTPSKDWLKIVRSSRARNKIRAWIKTEERVR
SISLGKEIMEKEFRRFSLNLGKLQKTGELKRVATEFGYVGDDDLMAAVGYGKLSCNQVLGKLLPEEKLAEKQELKESRIG
KAISKLTGKSTSAIQIGGVDDVLVRFGKCCNPVPGDEIVGFITRGRGVTVHTADCPIALENDPERRIAVDWNRERKAALP
VKIKVSCHDQKGILANITLAITNCEANISSASIQSTVDRRGVNIFEVDVTSLEHLTRVMNNIMKVSGVTHVERMKS
>Mature_716_residues
MIRLNDILDKVGSYNPAPDLDLVRKAYVYCAKVHQGQTRLSGEPYLVHPMEVAGLLADLRLDVPTVVTGLLHDTIEDTLT
TREELEGLFGAEVANLVDGVTKISKIHFKTKEESQAENFRKMLLAMANDIRVILVKLADRLHNMRTLQYQPEPKQRSIAR
ETLDIYAPIANRLGISWIKSELEDLSFRYLEPQIYYDLASKVTKKKKERESYVDEVRKIIVDKLVEHDIKGEVFGRSKHL
YSIWRKMQSRSVDIDQIYDLIAIRVMVNDIRECYEVLGLIHSTWKPIPGRFKDYIAMPKGNMYQSLHTTVIGPHGERMEV
QIRTYDMHGVAEAGIAAHWKYKEGKGYDEKEVKRFAWLRQLLEWQQELQDSHEFMSTVKVELFPEEVYVFTPNGDVKSFP
KGSTPIDFAYTIHTDLGHRCVGAKVNGKLVPLKYELKNGDIVEVITSPHHTPSKDWLKIVRSSRARNKIRAWIKTEERVR
SISLGKEIMEKEFRRFSLNLGKLQKTGELKRVATEFGYVGDDDLMAAVGYGKLSCNQVLGKLLPEEKLAEKQELKESRIG
KAISKLTGKSTSAIQIGGVDDVLVRFGKCCNPVPGDEIVGFITRGRGVTVHTADCPIALENDPERRIAVDWNRERKAALP
VKIKVSCHDQKGILANITLAITNCEANISSASIQSTVDRRGVNIFEVDVTSLEHLTRVMNNIMKVSGVTHVERMKS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=714, Percent_Identity=42.436974789916, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1789147, Length=744, Percent_Identity=35.4838709677419, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI25143118, Length=133, Percent_Identity=34.5864661654135, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 81334; Mature: 81334

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLNDILDKVGSYNPAPDLDLVRKAYVYCAKVHQGQTRLSGEPYLVHPMEVAGLLADLR
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHH
LDVPTVVTGLLHDTIEDTLTTREELEGLFGAEVANLVDGVTKISKIHFKTKEESQAENFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHH
KMLLAMANDIRVILVKLADRLHNMRTLQYQPEPKQRSIARETLDIYAPIANRLGISWIKS
HHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEDLSFRYLEPQIYYDLASKVTKKKKERESYVDEVRKIIVDKLVEHDIKGEVFGRSKHL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
YSIWRKMQSRSVDIDQIYDLIAIRVMVNDIRECYEVLGLIHSTWKPIPGRFKDYIAMPKG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCCC
NMYQSLHTTVIGPHGERMEVQIRTYDMHGVAEAGIAAHWKYKEGKGYDEKEVKRFAWLRQ
CHHHHHHHEEECCCCCEEEEEEEEEECCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHH
LLEWQQELQDSHEFMSTVKVELFPEEVYVFTPNGDVKSFPKGSTPIDFAYTIHTDLGHRC
HHHHHHHHHHHHHHHHHHEEEEEECEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCEE
VGAKVNGKLVPLKYELKNGDIVEVITSPHHTPSKDWLKIVRSSRARNKIRAWIKTEERVR
ECCEECCEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SISLGKEIMEKEFRRFSLNLGKLQKTGELKRVATEFGYVGDDDLMAAVGYGKLSCNQVLG
HHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCHHHEECCCCCCCHHHHHH
KLLPEEKLAEKQELKESRIGKAISKLTGKSTSAIQIGGVDDVLVRFGKCCNPVPGDEIVG
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCHHHEE
FITRGRGVTVHTADCPIALENDPERRIAVDWNRERKAALPVKIKVSCHDQKGILANITLA
EEECCCCEEEEECCCCEEECCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEEE
ITNCEANISSASIQSTVDRRGVNIFEVDVTSLEHLTRVMNNIMKVSGVTHVERMKS
EEECCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MIRLNDILDKVGSYNPAPDLDLVRKAYVYCAKVHQGQTRLSGEPYLVHPMEVAGLLADLR
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHH
LDVPTVVTGLLHDTIEDTLTTREELEGLFGAEVANLVDGVTKISKIHFKTKEESQAENFR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHH
KMLLAMANDIRVILVKLADRLHNMRTLQYQPEPKQRSIARETLDIYAPIANRLGISWIKS
HHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEDLSFRYLEPQIYYDLASKVTKKKKERESYVDEVRKIIVDKLVEHDIKGEVFGRSKHL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
YSIWRKMQSRSVDIDQIYDLIAIRVMVNDIRECYEVLGLIHSTWKPIPGRFKDYIAMPKG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCCC
NMYQSLHTTVIGPHGERMEVQIRTYDMHGVAEAGIAAHWKYKEGKGYDEKEVKRFAWLRQ
CHHHHHHHEEECCCCCEEEEEEEEEECCCHHHCCCEEEEEECCCCCCCHHHHHHHHHHHH
LLEWQQELQDSHEFMSTVKVELFPEEVYVFTPNGDVKSFPKGSTPIDFAYTIHTDLGHRC
HHHHHHHHHHHHHHHHHHEEEEEECEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCEE
VGAKVNGKLVPLKYELKNGDIVEVITSPHHTPSKDWLKIVRSSRARNKIRAWIKTEERVR
ECCEECCEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SISLGKEIMEKEFRRFSLNLGKLQKTGELKRVATEFGYVGDDDLMAAVGYGKLSCNQVLG
HHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCHHHEECCCCCCCHHHHHH
KLLPEEKLAEKQELKESRIGKAISKLTGKSTSAIQIGGVDDVLVRFGKCCNPVPGDEIVG
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCHHHEE
FITRGRGVTVHTADCPIALENDPERRIAVDWNRERKAALPVKIKVSCHDQKGILANITLA
EEECCCCEEEEECCCCEEECCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEEE
ITNCEANISSASIQSTVDRRGVNIFEVDVTSLEHLTRVMNNIMKVSGVTHVERMKS
EEECCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA