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The map label for this gene is metG [H]

Identifier: 78223524

GI number: 78223524

Start: 2629251

End: 2630780

Strand: Direct

Name: metG [H]

Synonym: Gmet_2321

Alternate gene names: 78223524

Gene position: 2629251-2630780 (Clockwise)

Preceding gene: 78223523

Following gene: 78223526

Centisome position: 65.77

GC content: 61.18

Gene sequence:

>1530_bases
ATGAGCCGAACGTTCTATGTCACGACCCCCATCTACTACGTAAATGACGTACCGCACATCGGCCATGCGTACACGACCCT
CGCCGCCGATGTCCTTGCCCGCTACAAGCGGCTCAAGGGGTATGATGTCTTTTTCCTGACCGGGACCGACGAGCACGGCC
AGAAGGTGGAGAAGGCCGCCAACGCCGCCGGCGAGACCCCCCTGGAGCTGGCCGATCGGGTGGTGAAGCGTTTCCAGGCC
CTCTGGGAAAAACTTGACATCAGCTACAACGACTTCATCCGCACCACCCAGGAGCGCCACAAGAAGGGGGTAACCCACCT
CTTCCGGGAGGTCATGGCCAAGGGGGACATCTATCTCGGCGAGTATGAGGACTGGTACTGCACCCCGTGCGAAACCTTCT
GGACCGAAACCCAGCTCATCGACGGCAAATGCCCCGACTGCAACCGTCCCGTGGAAAAGCTCAAGGAAGAGTCCTACTTC
TTCCGGATGAGCAAGTACCAGGAGCAGCTCCTGGCCCACATCGAAGCGAACCCCGACTTCATCCAGCCCAAGAGCCGGCG
CAACGAAATTATCTCTTTCGTCAAGGAAGGGCTCCGGGATCTCTCGGTTTCCCGCACCTCCTTCAACTGGGGGATTCCGG
TACCGGGGAACGAGCGGCACGTGATCTACGTCTGGTTCGACGCCCTCACCAACTACATCACAGCCCTCGGCTATCCCGAC
CAGGCCGGTAATTACGGGAAATTCTGGCCCGTGGACGCCCACCTCATCGGCAAGGATATCCTCCGCTTCCACGCGGTCTA
CTGGCCCACCTTCCTCATGGCCGCCGGACTTCCGGTGCCGAGGAAAGTATTTGCCCACGGCTGGTGGACCGTCGAAGGGC
AGAAGATGAGCAAGAGCCTCCAGAATGTGGTGGAGCCCAACATGCTCGTGGACAAGTACGGCATCGACGCGGTCCGCTAC
TTCCTCCTGCGGGAGGTCCCCTTCGGCCTCGACGGCGACTTCTCCCACGCGGCCCTCGTCCACCGGATCAACTCGGACCT
GGCCAACGACCTGGGGAACCTCCTCAGCCGCTCTACCGCCATGGTGAACAAGTACTTCGGCGGCACCCTCCCCCAGCCGG
GAGCCATGACCGAACTGGACGCCGCCTTCCGGGCCAGGACCGAGGCGATGATCAGCCAACTCGAGTCATGCATGGACGAA
ATGGCCTTCAGTAAGGGCCTCCAGGCAATCTGGGAAGTGATCTCCGCCGGAAACAAATACATCGACGAGAGCGCCCCCTG
GACCCTGGCCAAGGATCCGGCCCAGAAGGAGCGTCTCGGCACCATCATGTACACCCTCTTGGAGGCCCAGCGGATCGTGC
ATACGCTCTTGTCTGCCTTCATGCCGCGCACCGCGGCCAAAGCCCTCGGCTATCTGGGCTGCATGGAGGCACTCAGGGAC
GAAGACCTGGCGTGGGGCAAACTGGTTCCGGGAACACAGATTGCCAAGGCAGAGGCGCTTTTCCCGCGAATCGAAGACAA
AGCCGAATAG

Upstream 100 bases:

>100_bases
AGCGCGATACTAAAAAGAACCAGGACCAGCAGCGCGGAGAACGCGGCAACCGTGATCGACGCGGCGGCAAAGGCAGGGAC
AAGGATAAAAAGGAGCCGAA

Downstream 100 bases:

>100_bases
GTACAATGAGGGGCGCTGCTTGCTGCGCCCGTATTGCTGCCCCCATGGAGCAAGCGCAGACCCTACGGTCCCGCAGGTGG
TCTCAGTTCTCCAGAGGCGA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGYDVFFLTGTDEHGQKVEKAANAAGETPLELADRVVKRFQA
LWEKLDISYNDFIRTTQERHKKGVTHLFREVMAKGDIYLGEYEDWYCTPCETFWTETQLIDGKCPDCNRPVEKLKEESYF
FRMSKYQEQLLAHIEANPDFIQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNERHVIYVWFDALTNYITALGYPD
QAGNYGKFWPVDAHLIGKDILRFHAVYWPTFLMAAGLPVPRKVFAHGWWTVEGQKMSKSLQNVVEPNMLVDKYGIDAVRY
FLLREVPFGLDGDFSHAALVHRINSDLANDLGNLLSRSTAMVNKYFGGTLPQPGAMTELDAAFRARTEAMISQLESCMDE
MAFSKGLQAIWEVISAGNKYIDESAPWTLAKDPAQKERLGTIMYTLLEAQRIVHTLLSAFMPRTAAKALGYLGCMEALRD
EDLAWGKLVPGTQIAKAEALFPRIEDKAE

Sequences:

>Translated_509_residues
MSRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGYDVFFLTGTDEHGQKVEKAANAAGETPLELADRVVKRFQA
LWEKLDISYNDFIRTTQERHKKGVTHLFREVMAKGDIYLGEYEDWYCTPCETFWTETQLIDGKCPDCNRPVEKLKEESYF
FRMSKYQEQLLAHIEANPDFIQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNERHVIYVWFDALTNYITALGYPD
QAGNYGKFWPVDAHLIGKDILRFHAVYWPTFLMAAGLPVPRKVFAHGWWTVEGQKMSKSLQNVVEPNMLVDKYGIDAVRY
FLLREVPFGLDGDFSHAALVHRINSDLANDLGNLLSRSTAMVNKYFGGTLPQPGAMTELDAAFRARTEAMISQLESCMDE
MAFSKGLQAIWEVISAGNKYIDESAPWTLAKDPAQKERLGTIMYTLLEAQRIVHTLLSAFMPRTAAKALGYLGCMEALRD
EDLAWGKLVPGTQIAKAEALFPRIEDKAE
>Mature_508_residues
SRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGYDVFFLTGTDEHGQKVEKAANAAGETPLELADRVVKRFQAL
WEKLDISYNDFIRTTQERHKKGVTHLFREVMAKGDIYLGEYEDWYCTPCETFWTETQLIDGKCPDCNRPVEKLKEESYFF
RMSKYQEQLLAHIEANPDFIQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNERHVIYVWFDALTNYITALGYPDQ
AGNYGKFWPVDAHLIGKDILRFHAVYWPTFLMAAGLPVPRKVFAHGWWTVEGQKMSKSLQNVVEPNMLVDKYGIDAVRYF
LLREVPFGLDGDFSHAALVHRINSDLANDLGNLLSRSTAMVNKYFGGTLPQPGAMTELDAAFRARTEAMISQLESCMDEM
AFSKGLQAIWEVISAGNKYIDESAPWTLAKDPAQKERLGTIMYTLLEAQRIVHTLLSAFMPRTAAKALGYLGCMEALRDE
DLAWGKLVPGTQIAKAEALFPRIEDKAE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=504, Percent_Identity=39.8809523809524, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI14043022, Length=556, Percent_Identity=26.978417266187, Blast_Score=182, Evalue=7e-46,
Organism=Escherichia coli, GI1788432, Length=548, Percent_Identity=25.5474452554745, Blast_Score=200, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI212645096, Length=521, Percent_Identity=35.700575815739, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI71992781, Length=415, Percent_Identity=32.7710843373494, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17540794, Length=512, Percent_Identity=28.515625, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321610, Length=533, Percent_Identity=34.3339587242026, Blast_Score=303, Evalue=5e-83,
Organism=Saccharomyces cerevisiae, GI6321704, Length=556, Percent_Identity=26.4388489208633, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21358483, Length=498, Percent_Identity=37.9518072289157, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24655311, Length=554, Percent_Identity=28.8808664259928, Blast_Score=193, Evalue=3e-49,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57925; Mature: 57794

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGYDVFFLTGTDEHGQKVEKAA
CCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
NAAGETPLELADRVVKRFQALWEKLDISYNDFIRTTQERHKKGVTHLFREVMAKGDIYLG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
EYEDWYCTPCETFWTETQLIDGKCPDCNRPVEKLKEESYFFRMSKYQEQLLAHIEANPDF
CCCCCEECCHHHHHCHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNERHVIYVWFDALTNYITALGYPD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCC
QAGNYGKFWPVDAHLIGKDILRFHAVYWPTFLMAAGLPVPRKVFAHGWWTVEGQKMSKSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECHHHHHHHH
QNVVEPNMLVDKYGIDAVRYFLLREVPFGLDGDFSHAALVHRINSDLANDLGNLLSRSTA
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MVNKYFGGTLPQPGAMTELDAAFRARTEAMISQLESCMDEMAFSKGLQAIWEVISAGNKY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDESAPWTLAKDPAQKERLGTIMYTLLEAQRIVHTLLSAFMPRTAAKALGYLGCMEALRD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
EDLAWGKLVPGTQIAKAEALFPRIEDKAE
CCCCCCCCCCCCHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
SRTFYVTTPIYYVNDVPHIGHAYTTLAADVLARYKRLKGYDVFFLTGTDEHGQKVEKAA
CCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
NAAGETPLELADRVVKRFQALWEKLDISYNDFIRTTQERHKKGVTHLFREVMAKGDIYLG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
EYEDWYCTPCETFWTETQLIDGKCPDCNRPVEKLKEESYFFRMSKYQEQLLAHIEANPDF
CCCCCEECCHHHHHCHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IQPKSRRNEIISFVKEGLRDLSVSRTSFNWGIPVPGNERHVIYVWFDALTNYITALGYPD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCC
QAGNYGKFWPVDAHLIGKDILRFHAVYWPTFLMAAGLPVPRKVFAHGWWTVEGQKMSKSL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECHHHHHHHH
QNVVEPNMLVDKYGIDAVRYFLLREVPFGLDGDFSHAALVHRINSDLANDLGNLLSRSTA
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MVNKYFGGTLPQPGAMTELDAAFRARTEAMISQLESCMDEMAFSKGLQAIWEVISAGNKY
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDESAPWTLAKDPAQKERLGTIMYTLLEAQRIVHTLLSAFMPRTAAKALGYLGCMEALRD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
EDLAWGKLVPGTQIAKAEALFPRIEDKAE
CCCCCCCCCCCCHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]