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The map label for this gene is nreB [H]

Identifier: 78223366

GI number: 78223366

Start: 2432029

End: 2433348

Strand: Reverse

Name: nreB [H]

Synonym: Gmet_2159

Alternate gene names: 78223366

Gene position: 2433348-2432029 (Counterclockwise)

Preceding gene: 78223367

Following gene: 78223365

Centisome position: 60.87

GC content: 58.56

Gene sequence:

>1320_bases
GTGACGGAGCCCCTCCCTCCCTCGTCGAGCGCGTTTTCGCCTCGGACAGAGAAGGAGATAGAGATGCTGCTGCTTGAAAT
TGAAGCGCAGAAGAACGTGCTGGAACTGCAAAACGCCAAACTCGTTGATGCCATGGCCGAGAAAGAGCACGTTCTGCAAC
AATTCAATCGCCTGTTCGATCAGGCGCCGGTTGGCGGCGTTATCCTGGGACAGGATGGAATAATTCGCACCGTCAACCAT
ATTGCGGCCCACATGCTGGGGGAGGAGCGTTCCAGGCTCGTTGGCCGGCCATTGGAGCAGTTTCTTTCCGAGGGATCGCG
TCCGGTCTTTGCGGAATTTCTCAGGAACATGATGAGTGCGTGTAAAGCCAGTTGCGAGCTTGAACAGCCGATAGGGGGGA
ATCCCTCCCTGATCCGTTTCGAGGGGGCTTGCGACACCTGCGGGGACGAGTGCCGCGTCGCGATGATAGACATTACCGGC
CGGCACATTCCGGAGGAGGAGCGGGCACCCGGGGCGGGGGGGCACCGGAAGCTGCGTTGGTATGAATTGTCGGGAAAGCC
GCCTCCTCCGGACCGGGAGGATTCCCGTGAGATTGAGCGCAGCAAACGGGAGGAGGGGCTGGTCGATTCCCTGAAAAAGC
AGCGCAGGCTTTCGTTTCACCTTATGTCGGCCCGGGAGGAGGAGCGAACGGCAGTCGCGCGGGAAATTCACGAAGAACTG
GGACAGATGCTGGCGGCCCTTCAGTTGAACGTGTCGCTCGTGACAAAGGAATACCTCGACCACGCCAAGCTTGTCGCCAG
GACACAGGCGATGGAGCAGTTGATCACTTCGTCCATCATGACCGTGAACAGGATCTCCTCGGAACTGCGGCCGGTTATGC
TCGATATCCTGGGACTGGCCGATGCCATTGAATGGCAGGCAAAGGAATTCGAGAAGAAAAGCGGGATACGCTGCAAGACA
ACCATCCTTCTCGCCGAGAAGAAGGTGGATCGGGATGTGTCCACGGCGGTTTACCGGATAGTTCAGGAAGCGTTGAGCAA
TGTCGCCCGCCACGCCACCGCTGACCATGTCCGGGTGACCCTGGTGGAAAAAGTCGGTAGGGTGACCCTGTCGGTGAGCG
ACAATGGCCGGGGAATTACTGAGAGAGAGAAGAACAATCCGCAGTCGCTCGGTATTGCAGGGATGCGGGAACGGGCGGAG
GCGTTTGGAGGGAGACTGAGGATATGCGGCTCTTCGCGCCAGGGGACCACAGTGGTTGCACGGATTCCATTAGCCAGGGA
AGGAGACGTTGATGAGCACCAGGATACTTGTAGCCGATGA

Upstream 100 bases:

>100_bases
TATACTTATTAGCCGGTGTAGCGAGTATGCGAGGTCGCCATTGGAAACGGACAATCTCAAGAACAAGCCGCCAGTATCCG
CCGAATCGGAGCCGGGAAGA

Downstream 100 bases:

>100_bases
TCACGCAATCTTCAGGGAAGGGCTCAAGCAGGTTATTGGCACCACGGTGGACATGAGCGTTGTCGACGAAGCGGTCGACG
GCAAGGAGTTGCTGAACAAG

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTEPLPPSSSAFSPRTEKEIEMLLLEIEAQKNVLELQNAKLVDAMAEKEHVLQQFNRLFDQAPVGGVILGQDGIIRTVNH
IAAHMLGEERSRLVGRPLEQFLSEGSRPVFAEFLRNMMSACKASCELEQPIGGNPSLIRFEGACDTCGDECRVAMIDITG
RHIPEEERAPGAGGHRKLRWYELSGKPPPPDREDSREIERSKREEGLVDSLKKQRRLSFHLMSAREEERTAVAREIHEEL
GQMLAALQLNVSLVTKEYLDHAKLVARTQAMEQLITSSIMTVNRISSELRPVMLDILGLADAIEWQAKEFEKKSGIRCKT
TILLAEKKVDRDVSTAVYRIVQEALSNVARHATADHVRVTLVEKVGRVTLSVSDNGRGITEREKNNPQSLGIAGMRERAE
AFGGRLRICGSSRQGTTVVARIPLAREGDVDEHQDTCSR

Sequences:

>Translated_439_residues
MTEPLPPSSSAFSPRTEKEIEMLLLEIEAQKNVLELQNAKLVDAMAEKEHVLQQFNRLFDQAPVGGVILGQDGIIRTVNH
IAAHMLGEERSRLVGRPLEQFLSEGSRPVFAEFLRNMMSACKASCELEQPIGGNPSLIRFEGACDTCGDECRVAMIDITG
RHIPEEERAPGAGGHRKLRWYELSGKPPPPDREDSREIERSKREEGLVDSLKKQRRLSFHLMSAREEERTAVAREIHEEL
GQMLAALQLNVSLVTKEYLDHAKLVARTQAMEQLITSSIMTVNRISSELRPVMLDILGLADAIEWQAKEFEKKSGIRCKT
TILLAEKKVDRDVSTAVYRIVQEALSNVARHATADHVRVTLVEKVGRVTLSVSDNGRGITEREKNNPQSLGIAGMRERAE
AFGGRLRICGSSRQGTTVVARIPLAREGDVDEHQDTCSR
>Mature_438_residues
TEPLPPSSSAFSPRTEKEIEMLLLEIEAQKNVLELQNAKLVDAMAEKEHVLQQFNRLFDQAPVGGVILGQDGIIRTVNHI
AAHMLGEERSRLVGRPLEQFLSEGSRPVFAEFLRNMMSACKASCELEQPIGGNPSLIRFEGACDTCGDECRVAMIDITGR
HIPEEERAPGAGGHRKLRWYELSGKPPPPDREDSREIERSKREEGLVDSLKKQRRLSFHLMSAREEERTAVAREIHEELG
QMLAALQLNVSLVTKEYLDHAKLVARTQAMEQLITSSIMTVNRISSELRPVMLDILGLADAIEWQAKEFEKKSGIRCKTT
ILLAEKKVDRDVSTAVYRIVQEALSNVARHATADHVRVTLVEKVGRVTLSVSDNGRGITEREKNNPQSLGIAGMRERAEA
FGGRLRICGSSRQGTTVVARIPLAREGDVDEHQDTCSR

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=229, Percent_Identity=31.0043668122271, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787474, Length=251, Percent_Identity=28.2868525896414, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788812, Length=194, Percent_Identity=27.319587628866, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48968; Mature: 48836

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPLPPSSSAFSPRTEKEIEMLLLEIEAQKNVLELQNAKLVDAMAEKEHVLQQFNRLFD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QAPVGGVILGQDGIIRTVNHIAAHMLGEERSRLVGRPLEQFLSEGSRPVFAEFLRNMMSA
HCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHH
CKASCELEQPIGGNPSLIRFEGACDTCGDECRVAMIDITGRHIPEEERAPGAGGHRKLRW
HHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCEEEE
YELSGKPPPPDREDSREIERSKREEGLVDSLKKQRRLSFHLMSAREEERTAVAREIHEEL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQMLAALQLNVSLVTKEYLDHAKLVARTQAMEQLITSSIMTVNRISSELRPVMLDILGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAIEWQAKEFEKKSGIRCKTTILLAEKKVDRDVSTAVYRIVQEALSNVARHATADHVRVT
HHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LVEKVGRVTLSVSDNGRGITEREKNNPQSLGIAGMRERAEAFGGRLRICGSSRQGTTVVA
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEE
RIPLAREGDVDEHQDTCSR
ECCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
TEPLPPSSSAFSPRTEKEIEMLLLEIEAQKNVLELQNAKLVDAMAEKEHVLQQFNRLFD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QAPVGGVILGQDGIIRTVNHIAAHMLGEERSRLVGRPLEQFLSEGSRPVFAEFLRNMMSA
HCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHH
CKASCELEQPIGGNPSLIRFEGACDTCGDECRVAMIDITGRHIPEEERAPGAGGHRKLRW
HHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCEEEE
YELSGKPPPPDREDSREIERSKREEGLVDSLKKQRRLSFHLMSAREEERTAVAREIHEEL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQMLAALQLNVSLVTKEYLDHAKLVARTQAMEQLITSSIMTVNRISSELRPVMLDILGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAIEWQAKEFEKKSGIRCKTTILLAEKKVDRDVSTAVYRIVQEALSNVARHATADHVRVT
HHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LVEKVGRVTLSVSDNGRGITEREKNNPQSLGIAGMRERAEAFGGRLRICGSSRQGTTVVA
HHHHHCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEE
RIPLAREGDVDEHQDTCSR
ECCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]