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The map label for this gene is zraS [H]

Identifier: 78223353

GI number: 78223353

Start: 2415050

End: 2416861

Strand: Reverse

Name: zraS [H]

Synonym: Gmet_2146

Alternate gene names: 78223353

Gene position: 2416861-2415050 (Counterclockwise)

Preceding gene: 78223354

Following gene: 78223352

Centisome position: 60.46

GC content: 63.25

Gene sequence:

>1812_bases
ATGATTCATCTTCGCAAACTCAAGCTGCTGCTCGTTCTGGTCCTGGTGGTCGCCACGGCCGTCGGCGCTCTCCTCTGGAT
CAGGCACACCACCAACGCCGTTCGCGCCGAGGCGCGGGAGCGCTTCTTCGAGCAGTACAACCGGCAGCAGTCCCTCATGG
CCGAACTGGCCTCCCACACCCTGGAGGAGATGTTTGCCACGTTCCATCGCAACCTCGATCTGGTGGTGACGCTCTTCGAA
GGGAAGGAGGTCACCCGCCGGAGGGCCGAGGAAGTCGGCGAACGTCTGAAAAAGATCTACGGTTCCCTCGCCTCGACCCC
GGTTGTCGACCTCGTGATCTTTGACAGCCGTGGAACCGCCGTCGCCATAGAGCCGGCCGATCCGTACACCGTTGGCCGTA
ACTATGCCTGGCGGGACTATATAAAATGGGCCAAGGAAAAGGGGAAGCCCGGGGAGATGTACCTGTCCCCCTTCACCCGC
ATGGAGGGGGGAAAGCGCCGGGGGTACAAGGCCCTGATCGTGGCCGAGGGGATCTACGGACCCCGGGGGGAGTTCCTCGG
GGTGGCCAGTTGTGTCCTCGATTTCGAGAAGCTGGCGAACAAGCACATCCTTCCCATACGCGTCGGCACCCATGGCCGCG
CCTGGCTTGCCGACATCAGCAGCAGGACCATGCTCGTCGCCCCGAGCGGCAGGCTTGTGGGGCGCGGCTTCGACGCGGCG
TTTCTTCCCCGGTGGCCGCGCCTCTATAACCTTCTCATCTCTGTGGAAGAGGGGAAGCCGGGGAGCGGCTGGTACGACTA
CCTGGATGCCGAGATCCCCGATCAGCCGGTGCGGAAGCTGGGGAGCTACTACCCGTTCCGCATCGAGAACCGCCTCTGGG
CAATGGGGATTTCAACCCCCGAGCGGGAGGTGGACGAACTCCTGTCCACGTTCATGCATCGACAGGAGGCCTTCGCCACC
ACTCTGCTGGTGACGGTCCTGGCGGGGGCAACCCTGTTGATGGGGGTTCTCATGAATTGGAACCGGATCCTCTCCAGCCA
GGTCAACCATCACACCAAAGCCCTCAGTGAAGCCCATTCCCGGCTCGAATCCACCTTCGACGAGCTGCTGGTGGCGAAGA
AGGTGGCCGCGGTGGGGCACCTGGCCCTGGGGCTGGCCCATGAGATCCGCAACCCCCTCTCGGCCATCCAGATGAACATG
CAGATGATCCGGAAAAAAATCGCTCCGGCTGGCACGCTCAGGGAGAACTTTTCCATCGTGGAGGAGGAAATCCAGCGCCT
CAACCGCCTGCTGAACGATGTCCTGGATTTCGCCCGCTCGCGGCCCCTGCGCCTTGAGACGGCGGAACTGGAGGGGATTG
TCGACCGCCTGATGCAGCTCATGGCGCAGCGCCTGGAGGAAGAGCAGGTTCGGTCCGAAGTCCGTATCGATTCGCCCCTG
AACCTCGTCTGCGACCCCGAGCAGATTCATCAGGTGCTGCTGAACCTGGTCCTCAATGCCCTCGATGCCATGAACCGGAC
GCCGCCCGATGCCCGGCTGCTCACCATAACCGCCCAGAGTATGAGCGGGATGGCGCTGCTCAGGGTCAGCGATACCGGGG
GGGGGATTCCGCCCGACAAGCTCGAACAGCTCTTCGAGCCGTTTTTCACCACCAAGGCGTCCGGAGGCGGCTTGGGGCTC
TCTATCCTGCAAACGATTGTCCTTCGCCATGGCGGATCGGTATCCGTCGAGAGCGAACCCGGCCGGGGCGCCACGTTCAC
CGTGACTCTGCCCCTCGGGGGACCCGCCGAGAAAGGGGATGGCCTGCAATGA

Upstream 100 bases:

>100_bases
GATGCCATCGGCGCTCTCCCGCTGCCGGCAGAGGGGGTAGAAAAGATTCTGGGAGGGAATGCCGCCAGACTGCTGAATTT
TTAATGACTGCGACACTGCC

Downstream 100 bases:

>100_bases
GAACAATCCTGGTCGCCGATGACGACAGAGCCATCCGTCGGACCCTGGAGCTGCACCTTGCCGAGACGGGGTATACGGTT
CTCACCGCTGCCACCGGCAA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MIHLRKLKLLLVLVLVVATAVGALLWIRHTTNAVRAEARERFFEQYNRQQSLMAELASHTLEEMFATFHRNLDLVVTLFE
GKEVTRRRAEEVGERLKKIYGSLASTPVVDLVIFDSRGTAVAIEPADPYTVGRNYAWRDYIKWAKEKGKPGEMYLSPFTR
MEGGKRRGYKALIVAEGIYGPRGEFLGVASCVLDFEKLANKHILPIRVGTHGRAWLADISSRTMLVAPSGRLVGRGFDAA
FLPRWPRLYNLLISVEEGKPGSGWYDYLDAEIPDQPVRKLGSYYPFRIENRLWAMGISTPEREVDELLSTFMHRQEAFAT
TLLVTVLAGATLLMGVLMNWNRILSSQVNHHTKALSEAHSRLESTFDELLVAKKVAAVGHLALGLAHEIRNPLSAIQMNM
QMIRKKIAPAGTLRENFSIVEEEIQRLNRLLNDVLDFARSRPLRLETAELEGIVDRLMQLMAQRLEEEQVRSEVRIDSPL
NLVCDPEQIHQVLLNLVLNALDAMNRTPPDARLLTITAQSMSGMALLRVSDTGGGIPPDKLEQLFEPFFTTKASGGGLGL
SILQTIVLRHGGSVSVESEPGRGATFTVTLPLGGPAEKGDGLQ

Sequences:

>Translated_603_residues
MIHLRKLKLLLVLVLVVATAVGALLWIRHTTNAVRAEARERFFEQYNRQQSLMAELASHTLEEMFATFHRNLDLVVTLFE
GKEVTRRRAEEVGERLKKIYGSLASTPVVDLVIFDSRGTAVAIEPADPYTVGRNYAWRDYIKWAKEKGKPGEMYLSPFTR
MEGGKRRGYKALIVAEGIYGPRGEFLGVASCVLDFEKLANKHILPIRVGTHGRAWLADISSRTMLVAPSGRLVGRGFDAA
FLPRWPRLYNLLISVEEGKPGSGWYDYLDAEIPDQPVRKLGSYYPFRIENRLWAMGISTPEREVDELLSTFMHRQEAFAT
TLLVTVLAGATLLMGVLMNWNRILSSQVNHHTKALSEAHSRLESTFDELLVAKKVAAVGHLALGLAHEIRNPLSAIQMNM
QMIRKKIAPAGTLRENFSIVEEEIQRLNRLLNDVLDFARSRPLRLETAELEGIVDRLMQLMAQRLEEEQVRSEVRIDSPL
NLVCDPEQIHQVLLNLVLNALDAMNRTPPDARLLTITAQSMSGMALLRVSDTGGGIPPDKLEQLFEPFFTTKASGGGLGL
SILQTIVLRHGGSVSVESEPGRGATFTVTLPLGGPAEKGDGLQ
>Mature_603_residues
MIHLRKLKLLLVLVLVVATAVGALLWIRHTTNAVRAEARERFFEQYNRQQSLMAELASHTLEEMFATFHRNLDLVVTLFE
GKEVTRRRAEEVGERLKKIYGSLASTPVVDLVIFDSRGTAVAIEPADPYTVGRNYAWRDYIKWAKEKGKPGEMYLSPFTR
MEGGKRRGYKALIVAEGIYGPRGEFLGVASCVLDFEKLANKHILPIRVGTHGRAWLADISSRTMLVAPSGRLVGRGFDAA
FLPRWPRLYNLLISVEEGKPGSGWYDYLDAEIPDQPVRKLGSYYPFRIENRLWAMGISTPEREVDELLSTFMHRQEAFAT
TLLVTVLAGATLLMGVLMNWNRILSSQVNHHTKALSEAHSRLESTFDELLVAKKVAAVGHLALGLAHEIRNPLSAIQMNM
QMIRKKIAPAGTLRENFSIVEEEIQRLNRLLNDVLDFARSRPLRLETAELEGIVDRLMQLMAQRLEEEQVRSEVRIDSPL
NLVCDPEQIHQVLLNLVLNALDAMNRTPPDARLLTITAQSMSGMALLRVSDTGGGIPPDKLEQLFEPFFTTKASGGGLGL
SILQTIVLRHGGSVSVESEPGRGATFTVTLPLGGPAEKGDGLQ

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=35.2422907488987, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788549, Length=225, Percent_Identity=35.1111111111111, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI145693157, Length=258, Percent_Identity=28.6821705426357, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790300, Length=237, Percent_Identity=34.1772151898734, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789149, Length=294, Percent_Identity=27.5510204081633, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081816, Length=279, Percent_Identity=28.3154121863799, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=27.5720164609054, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1786600, Length=239, Percent_Identity=24.6861924686192, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786912, Length=266, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=25.2173913043478, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1786783, Length=224, Percent_Identity=25.8928571428571, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1789808, Length=226, Percent_Identity=24.7787610619469, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1787894, Length=253, Percent_Identity=28.0632411067194, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790567, Length=100, Percent_Identity=38, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=25, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=223, Percent_Identity=26.457399103139, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=202, Percent_Identity=25.7425742574257, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI87082128, Length=218, Percent_Identity=23.8532110091743, Blast_Score=62, Evalue=8e-11,
Organism=Escherichia coli, GI1788279, Length=247, Percent_Identity=24.2914979757085, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67229; Mature: 67229

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHLRKLKLLLVLVLVVATAVGALLWIRHTTNAVRAEARERFFEQYNRQQSLMAELASHT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEMFATFHRNLDLVVTLFEGKEVTRRRAEEVGERLKKIYGSLASTPVVDLVIFDSRGTA
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCE
VAIEPADPYTVGRNYAWRDYIKWAKEKGKPGEMYLSPFTRMEGGKRRGYKALIVAEGIYG
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCCEEEEEECCCCC
PRGEFLGVASCVLDFEKLANKHILPIRVGTHGRAWLADISSRTMLVAPSGRLVGRGFDAA
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEECCCCEECCCCCCH
FLPRWPRLYNLLISVEEGKPGSGWYDYLDAEIPDQPVRKLGSYYPFRIENRLWAMGISTP
HCCCCHHHHHHHEEEECCCCCCCHHHHCCCCCCCHHHHHHCCCCCEEECCCEEEECCCCC
EREVDELLSTFMHRQEAFATTLLVTVLAGATLLMGVLMNWNRILSSQVNHHTKALSEAHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLESTFDELLVAKKVAAVGHLALGLAHEIRNPLSAIQMNMQMIRKKIAPAGTLRENFSIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EEEIQRLNRLLNDVLDFARSRPLRLETAELEGIVDRLMQLMAQRLEEEQVRSEVRIDSPL
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NLVCDPEQIHQVLLNLVLNALDAMNRTPPDARLLTITAQSMSGMALLRVSDTGGGIPPDK
CEEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHH
LEQLFEPFFTTKASGGGLGLSILQTIVLRHGGSVSVESEPGRGATFTVTLPLGGPAEKGD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCCC
GLQ
CCC
>Mature Secondary Structure
MIHLRKLKLLLVLVLVVATAVGALLWIRHTTNAVRAEARERFFEQYNRQQSLMAELASHT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEMFATFHRNLDLVVTLFEGKEVTRRRAEEVGERLKKIYGSLASTPVVDLVIFDSRGTA
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCE
VAIEPADPYTVGRNYAWRDYIKWAKEKGKPGEMYLSPFTRMEGGKRRGYKALIVAEGIYG
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCCEEEEEECCCCC
PRGEFLGVASCVLDFEKLANKHILPIRVGTHGRAWLADISSRTMLVAPSGRLVGRGFDAA
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEECCCCEECCCCCCH
FLPRWPRLYNLLISVEEGKPGSGWYDYLDAEIPDQPVRKLGSYYPFRIENRLWAMGISTP
HCCCCHHHHHHHEEEECCCCCCCHHHHCCCCCCCHHHHHHCCCCCEEECCCEEEECCCCC
EREVDELLSTFMHRQEAFATTLLVTVLAGATLLMGVLMNWNRILSSQVNHHTKALSEAHS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLESTFDELLVAKKVAAVGHLALGLAHEIRNPLSAIQMNMQMIRKKIAPAGTLRENFSIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EEEIQRLNRLLNDVLDFARSRPLRLETAELEGIVDRLMQLMAQRLEEEQVRSEVRIDSPL
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NLVCDPEQIHQVLLNLVLNALDAMNRTPPDARLLTITAQSMSGMALLRVSDTGGGIPPDK
CEEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHH
LEQLFEPFFTTKASGGGLGLSILQTIVLRHGGSVSVESEPGRGATFTVTLPLGGPAEKGD
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCCC
GLQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]