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The map label for this gene is zraR [H]

Identifier: 78223352

GI number: 78223352

Start: 2413713

End: 2415053

Strand: Reverse

Name: zraR [H]

Synonym: Gmet_2145

Alternate gene names: 78223352

Gene position: 2415053-2413713 (Counterclockwise)

Preceding gene: 78223353

Following gene: 78223349

Centisome position: 60.42

GC content: 61.3

Gene sequence:

>1341_bases
ATGAGAACAATCCTGGTCGCCGATGACGACAGAGCCATCCGTCGGACCCTGGAGCTGCACCTTGCCGAGACGGGGTATAC
GGTTCTCACCGCTGCCACCGGCAACGAAGCGGTCGACCTGGCCCTGGCGCGGGACGTGGGCCTGGTGCTCCTCGATCTGC
GGCTGACCGGCATGGACGGTTTCGAGGTGCTGACCCTGATCAAGGAAAGAAAGCCGGCCCTGCCGGTTATCATGATCACC
GCCTACGACGACATGCAGACCGCCATCGAGGCAATCCGCCTCGGGGCCATCGACCACCTGGGCAAGCCCCTCGATCTCGA
TCAGCTCGACCAGATTATCGACAAGATATTCGAGATGAGTGCCCTTTCCCGGACCGGCGTCGCCTTCCGCGACTCTATCG
ATGCCCCCTTTGAGCAGAACATCATGGTGGGCCGCTCCCGGGCCATGAAGGAGGTTTACAAAACCATCGGCGCCATTGCC
GACTTCCGGGCCACGGTCCTGATCCAGGGGGAGAGCGGCACCGGCAAGGAAATGGTTGCCCGTGCCATCCATTTCAACAG
CAAGTTCCGCAACCGTCCGTTCATCGCCGTGGCCTGCTCCGCCCTCTCGCCGACGGTCCTCGAAAGCGAGCTGTTCGGCC
ATGAGAAGGGGGCGTTCACCGGGGCGTATCGAACCAAACCGGGGAAGTTCGAGCTGGCCCAGGGGGGGACCCTGTTCCTT
GATGAGATATCGGAGATCAGTCCCGAGTTGCAGGTGAAACTGCTCCGCTTTATCCAGGAGCGGGAGTTCGAGCGGGTAGG
GGGAATCGAGACCATCAAGGCCGATGTGCGCATTGTGGCCGCCACCAACAAGAATCTCCTCACGCTGGTCAACAGCGGCA
TTTTCCGCCACGACCTGTACTACCGCCTGAAAGTGGTCTCCATCGATCTCCCTCCCCTCAGGGAACGCAGGGACGACATT
CCCATCCTGGTGAAATTCCTGCTGGAAAAGCTTCACCATGAGACCGGGAAGCGGGTGGATATCATCCCCCAGGAGACCAT
GAATGCCATCCTGGCCCATCCGTGGGCGGGCAACGTCCGGGAGCTGGAGAACACGCTGCGGCGCGCCGTCCTCCTTTCGC
ACGGCTCGGTGCTGTCGCCCGAATCCCTGCACCTGGAAGAGGAAAGCGAGAGCGACAGCTTTCCCCTGGTGCTCAGGCCG
CTCCATGAGGTCGAGCGGGAACATATCCAGAATGTCCTTGCCTTTACCCACAACGAGAAGAAGAGGGCGGCGGAAATCCT
CGGCATTTCCCGGCCGACCCTCGACCGCCGCATCAAGGAGTATGGTCTGGAAGATTCGTAG

Upstream 100 bases:

>100_bases
ATGGCGGATCGGTATCCGTCGAGAGCGAACCCGGCCGGGGCGCCACGTTCACCGTGACTCTGCCCCTCGGGGGACCCGCC
GAGAAAGGGGATGGCCTGCA

Downstream 100 bases:

>100_bases
CCTGTTCCGGTTTCACAAAAAAAAGCCCCCTATCCCGGGAAGGATACGAGGGCTCCATGACACAGCAATGCATTCTTCTA
CTTGGCCATGGCTTCAAACC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MRTILVADDDRAIRRTLELHLAETGYTVLTAATGNEAVDLALARDVGLVLLDLRLTGMDGFEVLTLIKERKPALPVIMIT
AYDDMQTAIEAIRLGAIDHLGKPLDLDQLDQIIDKIFEMSALSRTGVAFRDSIDAPFEQNIMVGRSRAMKEVYKTIGAIA
DFRATVLIQGESGTGKEMVARAIHFNSKFRNRPFIAVACSALSPTVLESELFGHEKGAFTGAYRTKPGKFELAQGGTLFL
DEISEISPELQVKLLRFIQEREFERVGGIETIKADVRIVAATNKNLLTLVNSGIFRHDLYYRLKVVSIDLPPLRERRDDI
PILVKFLLEKLHHETGKRVDIIPQETMNAILAHPWAGNVRELENTLRRAVLLSHGSVLSPESLHLEEESESDSFPLVLRP
LHEVEREHIQNVLAFTHNEKKRAAEILGISRPTLDRRIKEYGLEDS

Sequences:

>Translated_446_residues
MRTILVADDDRAIRRTLELHLAETGYTVLTAATGNEAVDLALARDVGLVLLDLRLTGMDGFEVLTLIKERKPALPVIMIT
AYDDMQTAIEAIRLGAIDHLGKPLDLDQLDQIIDKIFEMSALSRTGVAFRDSIDAPFEQNIMVGRSRAMKEVYKTIGAIA
DFRATVLIQGESGTGKEMVARAIHFNSKFRNRPFIAVACSALSPTVLESELFGHEKGAFTGAYRTKPGKFELAQGGTLFL
DEISEISPELQVKLLRFIQEREFERVGGIETIKADVRIVAATNKNLLTLVNSGIFRHDLYYRLKVVSIDLPPLRERRDDI
PILVKFLLEKLHHETGKRVDIIPQETMNAILAHPWAGNVRELENTLRRAVLLSHGSVLSPESLHLEEESESDSFPLVLRP
LHEVEREHIQNVLAFTHNEKKRAAEILGISRPTLDRRIKEYGLEDS
>Mature_446_residues
MRTILVADDDRAIRRTLELHLAETGYTVLTAATGNEAVDLALARDVGLVLLDLRLTGMDGFEVLTLIKERKPALPVIMIT
AYDDMQTAIEAIRLGAIDHLGKPLDLDQLDQIIDKIFEMSALSRTGVAFRDSIDAPFEQNIMVGRSRAMKEVYKTIGAIA
DFRATVLIQGESGTGKEMVARAIHFNSKFRNRPFIAVACSALSPTVLESELFGHEKGAFTGAYRTKPGKFELAQGGTLFL
DEISEISPELQVKLLRFIQEREFERVGGIETIKADVRIVAATNKNLLTLVNSGIFRHDLYYRLKVVSIDLPPLRERRDDI
PILVKFLLEKLHHETGKRVDIIPQETMNAILAHPWAGNVRELENTLRRAVLLSHGSVLSPESLHLEEESESDSFPLVLRP
LHEVEREHIQNVLAFTHNEKKRAAEILGISRPTLDRRIKEYGLEDS

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=435, Percent_Identity=45.5172413793103, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI1788550, Length=451, Percent_Identity=41.6851441241685, Blast_Score=347, Evalue=9e-97,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=39.1025641025641, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788905, Length=428, Percent_Identity=38.3177570093458, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=44.2367601246106, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789087, Length=329, Percent_Identity=44.6808510638298, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789233, Length=359, Percent_Identity=40.3899721448468, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI87082152, Length=330, Percent_Identity=40.3030303030303, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1786524, Length=342, Percent_Identity=39.766081871345, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87081872, Length=315, Percent_Identity=38.7301587301587, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1787583, Length=308, Percent_Identity=34.7402597402597, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081858, Length=285, Percent_Identity=32.9824561403509, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789828, Length=225, Percent_Identity=36.4444444444444, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87082012, Length=109, Percent_Identity=33.0275229357798, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789809, Length=107, Percent_Identity=35.5140186915888, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50022; Mature: 50022

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTILVADDDRAIRRTLELHLAETGYTVLTAATGNEAVDLALARDVGLVLLDLRLTGMDG
CCEEEEECCCHHHHHHHHHHEECCCCEEEEEECCCCCEEEEHHCCCCEEEEEEEECCCCH
FEVLTLIKERKPALPVIMITAYDDMQTAIEAIRLGAIDHLGKPLDLDQLDQIIDKIFEMS
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ALSRTGVAFRDSIDAPFEQNIMVGRSRAMKEVYKTIGAIADFRATVLIQGESGTGKEMVA
HHHHCCCHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHH
RAIHFNSKFRNRPFIAVACSALSPTVLESELFGHEKGAFTGAYRTKPGKFELAQGGTLFL
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCEECCCCCEEECCCCEEEE
DEISEISPELQVKLLRFIQEREFERVGGIETIKADVRIVAATNKNLLTLVNSGIFRHDLY
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEECCCCEEEHHHCCCHHEEEE
YRLKVVSIDLPPLRERRDDIPILVKFLLEKLHHETGKRVDIIPQETMNAILAHPWAGNVR
EEEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCHH
ELENTLRRAVLLSHGSVLSPESLHLEEESESDSFPLVLRPLHEVEREHIQNVLAFTHNEK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHH
KRAAEILGISRPTLDRRIKEYGLEDS
HHHHHHHCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MRTILVADDDRAIRRTLELHLAETGYTVLTAATGNEAVDLALARDVGLVLLDLRLTGMDG
CCEEEEECCCHHHHHHHHHHEECCCCEEEEEECCCCCEEEEHHCCCCEEEEEEEECCCCH
FEVLTLIKERKPALPVIMITAYDDMQTAIEAIRLGAIDHLGKPLDLDQLDQIIDKIFEMS
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ALSRTGVAFRDSIDAPFEQNIMVGRSRAMKEVYKTIGAIADFRATVLIQGESGTGKEMVA
HHHHCCCHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHH
RAIHFNSKFRNRPFIAVACSALSPTVLESELFGHEKGAFTGAYRTKPGKFELAQGGTLFL
HHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCEECCCCCEEECCCCEEEE
DEISEISPELQVKLLRFIQEREFERVGGIETIKADVRIVAATNKNLLTLVNSGIFRHDLY
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEECCCCEEEHHHCCCHHEEEE
YRLKVVSIDLPPLRERRDDIPILVKFLLEKLHHETGKRVDIIPQETMNAILAHPWAGNVR
EEEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCHH
ELENTLRRAVLLSHGSVLSPESLHLEEESESDSFPLVLRPLHEVEREHIQNVLAFTHNEK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHH
KRAAEILGISRPTLDRRIKEYGLEDS
HHHHHHHCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]