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The map label for this gene is buk [H]

Identifier: 78223335

GI number: 78223335

Start: 2396057

End: 2397145

Strand: Direct

Name: buk [H]

Synonym: Gmet_2128

Alternate gene names: 78223335

Gene position: 2396057-2397145 (Clockwise)

Preceding gene: 78223334

Following gene: 78223336

Centisome position: 59.94

GC content: 58.95

Gene sequence:

>1089_bases
ATGGACGACAAGAAAATACTGATAATCAATCCAGGCTCCACCTCGACGAAAATCGGTGTCTTTACCGAAGGGAAGATGAT
CGTCAATCAATCGGTGAAGCATGACGACGAGGAACTGAGGAAGTTCCCCACCATCTGGGACCAGTATGATTTCCGCCGGG
ATGCAATCTTCAAGGTGCTCGCGGACAACAACCTCTCCATGGGGGAAATTGACGCCATCGCCTGCCGGGGCGGCAACGTC
AGGCCCCTTCCCGGCGGCATCTACCGGGTCTGCCCGAAGATGATCGAGGACATGAAGAGCGGCATCTATGGCGGCCATCC
CATCAATGTGGGGGGGCTGGTGGCCTTCGACCTGGGGAACCAGTTCAATATCCCGGTCCTCACCGCCGATCCCCCCATGA
CCGACGAACTCTGCGCCTCGGCCCGGTACAGCGGCATTCCCCAGATCACCAGACAGAGCAGTTTCCACGCCCTCAACCAG
AAGGCCACTGCCAGGAAGATCGCCGCGGAACTGGGAAAGAAATACGACGAAATCAACCTGATCGTCGCCCACCTGGGTGG
CGGAATTTCGGTGGGCGCCCACCGCAGAGGAAAGATCATCGATGTGAATAACGCCCTTGACGGCGACGGCCCCTTCTCCC
CGGAGAGGGCCGGCTCCCTTCCGGCGGGCGACCTGGTGAAGCTCTGCTTCAGCGGCGAGTACACCAAAAAAGAGGTTCTC
AAGCTACTGACCGGAAAGGGAGGACTGTTCGCCTATATGGGAACGACCGACGCCGTCGAAATCGAGAAGAGAATCGCCCA
TGGTGGGCAGAAGGCGGCGGAGGTCTATGAAGCGATGATCTACCAGGTGGCCAAGGAGATCGGTGCCAGCGCGGCGGTCC
TTGAGGGAGATGTTGACGCCATCGCCCTCACCGGCAGCCTGGCCTACTCGAAAAAGCTCGTGGAAAGCCTGAGCAGGAAG
ATTTCCTTCATCGCCCCGGTGCTCCTCAATCCGGGCGAAAACGAAATGGAAGCCCTGGCCGACGCGGCCATGAGGTATTT
CAACAATGAAGAGGCCCTCTCCGTCTACCACCAGGACAAGGCGGCATGA

Upstream 100 bases:

>100_bases
GCCGCCGAGAACCGTATCGAAGCCAACCAGCTCTGGATCGATCTGGTCTATTCATTCTAGGGATTTCACAGGGATTGACA
CTCTTCCGGAGGGTACAACG

Downstream 100 bases:

>100_bases
CCAGAAAACTGTCTGAAGAGGATTTAGGCCAATAAACCAATAAAGGAGAGCAAGACCATGGGAAAACTTACGGGAAAAGT
AGCGATCATCACCGGCGGTG

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MDDKKILIINPGSTSTKIGVFTEGKMIVNQSVKHDDEELRKFPTIWDQYDFRRDAIFKVLADNNLSMGEIDAIACRGGNV
RPLPGGIYRVCPKMIEDMKSGIYGGHPINVGGLVAFDLGNQFNIPVLTADPPMTDELCASARYSGIPQITRQSSFHALNQ
KATARKIAAELGKKYDEINLIVAHLGGGISVGAHRRGKIIDVNNALDGDGPFSPERAGSLPAGDLVKLCFSGEYTKKEVL
KLLTGKGGLFAYMGTTDAVEIEKRIAHGGQKAAEVYEAMIYQVAKEIGASAAVLEGDVDAIALTGSLAYSKKLVESLSRK
ISFIAPVLLNPGENEMEALADAAMRYFNNEEALSVYHQDKAA

Sequences:

>Translated_362_residues
MDDKKILIINPGSTSTKIGVFTEGKMIVNQSVKHDDEELRKFPTIWDQYDFRRDAIFKVLADNNLSMGEIDAIACRGGNV
RPLPGGIYRVCPKMIEDMKSGIYGGHPINVGGLVAFDLGNQFNIPVLTADPPMTDELCASARYSGIPQITRQSSFHALNQ
KATARKIAAELGKKYDEINLIVAHLGGGISVGAHRRGKIIDVNNALDGDGPFSPERAGSLPAGDLVKLCFSGEYTKKEVL
KLLTGKGGLFAYMGTTDAVEIEKRIAHGGQKAAEVYEAMIYQVAKEIGASAAVLEGDVDAIALTGSLAYSKKLVESLSRK
ISFIAPVLLNPGENEMEALADAAMRYFNNEEALSVYHQDKAA
>Mature_362_residues
MDDKKILIINPGSTSTKIGVFTEGKMIVNQSVKHDDEELRKFPTIWDQYDFRRDAIFKVLADNNLSMGEIDAIACRGGNV
RPLPGGIYRVCPKMIEDMKSGIYGGHPINVGGLVAFDLGNQFNIPVLTADPPMTDELCASARYSGIPQITRQSSFHALNQ
KATARKIAAELGKKYDEINLIVAHLGGGISVGAHRRGKIIDVNNALDGDGPFSPERAGSLPAGDLVKLCFSGEYTKKEVL
KLLTGKGGLFAYMGTTDAVEIEKRIAHGGQKAAEVYEAMIYQVAKEIGASAAVLEGDVDAIALTGSLAYSKKLVESLSRK
ISFIAPVLLNPGENEMEALADAAMRYFNNEEALSVYHQDKAA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=172, Percent_Identity=29.6511627906977, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 39080; Mature: 39080

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDKKILIINPGSTSTKIGVFTEGKMIVNQSVKHDDEELRKFPTIWDQYDFRRDAIFKVL
CCCCEEEEECCCCCCCEEEEEECCCEEECCCCCCCHHHHHHCCCHHHHCCCHHHHHHHEE
ADNNLSMGEIDAIACRGGNVRPLPGGIYRVCPKMIEDMKSGIYGGHPINVGGLVAFDLGN
ECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
QFNIPVLTADPPMTDELCASARYSGIPQITRQSSFHALNQKATARKIAAELGKKYDEINL
CCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEE
IVAHLGGGISVGAHRRGKIIDVNNALDGDGPFSPERAGSLPAGDLVKLCFSGEYTKKEVL
EEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
KLLTGKGGLFAYMGTTDAVEIEKRIAHGGQKAAEVYEAMIYQVAKEIGASAAVLEGDVDA
HHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
IALTGSLAYSKKLVESLSRKISFIAPVLLNPGENEMEALADAAMRYFNNEEALSVYHQDK
EEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEEHHCCC
AA
CC
>Mature Secondary Structure
MDDKKILIINPGSTSTKIGVFTEGKMIVNQSVKHDDEELRKFPTIWDQYDFRRDAIFKVL
CCCCEEEEECCCCCCCEEEEEECCCEEECCCCCCCHHHHHHCCCHHHHCCCHHHHHHHEE
ADNNLSMGEIDAIACRGGNVRPLPGGIYRVCPKMIEDMKSGIYGGHPINVGGLVAFDLGN
ECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
QFNIPVLTADPPMTDELCASARYSGIPQITRQSSFHALNQKATARKIAAELGKKYDEINL
CCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEE
IVAHLGGGISVGAHRRGKIIDVNNALDGDGPFSPERAGSLPAGDLVKLCFSGEYTKKEVL
EEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
KLLTGKGGLFAYMGTTDAVEIEKRIAHGGQKAAEVYEAMIYQVAKEIGASAAVLEGDVDA
HHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCE
IALTGSLAYSKKLVESLSRKISFIAPVLLNPGENEMEALADAAMRYFNNEEALSVYHQDK
EEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEEHHCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA