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The map label for this gene is buk2 [H]

Identifier: 78223313

GI number: 78223313

Start: 2372028

End: 2373107

Strand: Direct

Name: buk2 [H]

Synonym: Gmet_2106

Alternate gene names: 78223313

Gene position: 2372028-2373107 (Clockwise)

Preceding gene: 78223312

Following gene: 78223314

Centisome position: 59.34

GC content: 63.06

Gene sequence:

>1080_bases
ATGAACCGTGACAAGACAATACTCATCATCAATCCCGGCTCCACCTCGACCAAGATCGGCATCTTTTCGGCAGGCGAGAT
GAAAATCAACGTGTCGGTCAGACATGACGACGCGGAGCTGAGGAAGTTTGCCACCATCTGGGATCAGTACGATTACCGCA
AGGACGCCCTCCTCGGGTTCCTGCGGGACCACGATCTCGCCATGGATGATATCGACGCCATCGCCTGCCGCGGCGGTAAC
GTCAGACCGCTCCCCGGTGGCATCTACCGGCTCTGTCCGCGAATGATCGCCGACATGAAGAGCGGCATCTACGGCGGCCA
CCCGATCAACGTGGGGGGGCTGGTGGCATATGACCTGGGGAACCAGTACGGCATCCCGGTTCTGACCGCCGATCCTCCCA
TGACCGACGAACTCTGCGCCTCGGCCCGCTACTCCGGGATTCCCCAGCTCACGAGGACGAGCAGCTTCCACGCCCTGAAC
CAGAAGGCCACTGCCCGCAGAATCGCGGCGGAACTGGGGAAACGGTACGATGAGGTGAACCTGATCGTCGCTCACCTGGG
GGGCGGCATCTCGGTGGGAGCACACCAGAAGGGGAAGATCGTCGACGTGAACAACGCCCTGGACGGGGACGGCCCCTTCT
CGCCGGAACGGGCCGGGACACTGCCGGCCGGCGACCTGGTCAAACTCTGCTTCAGCGGCGACTACACGAAGGCCGAGGTG
CTGAAGCTCCTGACCGGCGGCGGAGGGCTGTACGCCTATCTGGGTACCACCAACGTGATCGAGATTGAAAAGCGGATCAC
GGCGGGCGACGGGAAGGCGGCCGAGGTGTTCGAGGCGATGATCTACCAGGTGGCCAAGGAGATCGGCGCCTGCGCGGCCG
TACTGGAGGGGACGGTGGACGCCATCGCCCTCACCGGGAGCCTGGTCTATTCGGAGCGACTCCTGGAGAGTCTGAAGAGG
AAGATCGCTTTCATCGCCCCGGTCCATTTGAACCCCGGCGAGAACGAGATGGAGGCCCTGGCCGATGCGGCAATGCGCTA
CTTCAGCCAGGAAGAAGAGCTGTCCGTGTACGCCGCCTGA

Upstream 100 bases:

>100_bases
TCTCTTCAAGCGCTGGCACGGGGACAACCGGAAGGAGCAGGTTCGGGAGTCCCTCAGAATCGCCAATTAATGCCACATCC
AGGGACTGTGGGGGCAGACC

Downstream 100 bases:

>100_bases
CCGGTTGCAACGGGCGGCAATCAATTACATCATTTACGAGAAAGAGGATTACTTATGAAACAGTTCTCCCTCCGTCGGCT
GTTGTTCCTCCCTTGCATCG

Product: butyrate kinase

Products: NA

Alternate protein names: BK 2; BKII [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNRDKTILIINPGSTSTKIGIFSAGEMKINVSVRHDDAELRKFATIWDQYDYRKDALLGFLRDHDLAMDDIDAIACRGGN
VRPLPGGIYRLCPRMIADMKSGIYGGHPINVGGLVAYDLGNQYGIPVLTADPPMTDELCASARYSGIPQLTRTSSFHALN
QKATARRIAAELGKRYDEVNLIVAHLGGGISVGAHQKGKIVDVNNALDGDGPFSPERAGTLPAGDLVKLCFSGDYTKAEV
LKLLTGGGGLYAYLGTTNVIEIEKRITAGDGKAAEVFEAMIYQVAKEIGACAAVLEGTVDAIALTGSLVYSERLLESLKR
KIAFIAPVHLNPGENEMEALADAAMRYFSQEEELSVYAA

Sequences:

>Translated_359_residues
MNRDKTILIINPGSTSTKIGIFSAGEMKINVSVRHDDAELRKFATIWDQYDYRKDALLGFLRDHDLAMDDIDAIACRGGN
VRPLPGGIYRLCPRMIADMKSGIYGGHPINVGGLVAYDLGNQYGIPVLTADPPMTDELCASARYSGIPQLTRTSSFHALN
QKATARRIAAELGKRYDEVNLIVAHLGGGISVGAHQKGKIVDVNNALDGDGPFSPERAGTLPAGDLVKLCFSGDYTKAEV
LKLLTGGGGLYAYLGTTNVIEIEKRITAGDGKAAEVFEAMIYQVAKEIGACAAVLEGTVDAIALTGSLVYSERLLESLKR
KIAFIAPVHLNPGENEMEALADAAMRYFSQEEELSVYAA
>Mature_359_residues
MNRDKTILIINPGSTSTKIGIFSAGEMKINVSVRHDDAELRKFATIWDQYDYRKDALLGFLRDHDLAMDDIDAIACRGGN
VRPLPGGIYRLCPRMIADMKSGIYGGHPINVGGLVAYDLGNQYGIPVLTADPPMTDELCASARYSGIPQLTRTSSFHALN
QKATARRIAAELGKRYDEVNLIVAHLGGGISVGAHQKGKIVDVNNALDGDGPFSPERAGTLPAGDLVKLCFSGDYTKAEV
LKLLTGGGGLYAYLGTTNVIEIEKRITAGDGKAAEVFEAMIYQVAKEIGACAAVLEGTVDAIALTGSLVYSERLLESLKR
KIAFIAPVHLNPGENEMEALADAAMRYFSQEEELSVYAA

Specific function: Catalyzes the conversion of butyryl-CoA through butyryl phosphate to butyrate [H]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 38572; Mature: 38572

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRDKTILIINPGSTSTKIGIFSAGEMKINVSVRHDDAELRKFATIWDQYDYRKDALLGF
CCCCCEEEEEECCCCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHH
LRDHDLAMDDIDAIACRGGNVRPLPGGIYRLCPRMIADMKSGIYGGHPINVGGLVAYDLG
HHCCCCCHHCCHHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
NQYGIPVLTADPPMTDELCASARYSGIPQLTRTSSFHALNQKATARRIAAELGKRYDEVN
CCCCCEEEECCCCCCHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHE
LIVAHLGGGISVGAHQKGKIVDVNNALDGDGPFSPERAGTLPAGDLVKLCFSGDYTKAEV
EEEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
LKLLTGGGGLYAYLGTTNVIEIEKRITAGDGKAAEVFEAMIYQVAKEIGACAAVLEGTVD
HHHHCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AIALTGSLVYSERLLESLKRKIAFIAPVHLNPGENEMEALADAAMRYFSQEEELSVYAA
HHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MNRDKTILIINPGSTSTKIGIFSAGEMKINVSVRHDDAELRKFATIWDQYDYRKDALLGF
CCCCCEEEEEECCCCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHH
LRDHDLAMDDIDAIACRGGNVRPLPGGIYRLCPRMIADMKSGIYGGHPINVGGLVAYDLG
HHCCCCCHHCCHHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
NQYGIPVLTADPPMTDELCASARYSGIPQLTRTSSFHALNQKATARRIAAELGKRYDEVN
CCCCCEEEECCCCCCHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHE
LIVAHLGGGISVGAHQKGKIVDVNNALDGDGPFSPERAGTLPAGDLVKLCFSGDYTKAEV
EEEEECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
LKLLTGGGGLYAYLGTTNVIEIEKRITAGDGKAAEVFEAMIYQVAKEIGACAAVLEGTVD
HHHHCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AIALTGSLVYSERLLESLKRKIAFIAPVHLNPGENEMEALADAAMRYFSQEEELSVYAA
HHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286; 10937485 [H]