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The map label for this gene is fixA [H]

Identifier: 78223274

GI number: 78223274

Start: 2324930

End: 2325742

Strand: Reverse

Name: fixA [H]

Synonym: Gmet_2067

Alternate gene names: 78223274

Gene position: 2325742-2324930 (Counterclockwise)

Preceding gene: 78223275

Following gene: 78223273

Centisome position: 58.18

GC content: 63.59

Gene sequence:

>813_bases
ATGCTCGTGATCTCCTGCATCAAACAGGTGCCCGACACCACCCAGGTTAAGATCGACCCGGTGACCAACACTCTGGTCCG
GGAAGGGATCCCCTTCATCGTCAACCCCTACGACACCCATGCCCTGGAGGAGGGGTTGAATCTCAAGGACCGCTACGGCC
TGCGGTCGGTGGCCATCTCCATGGGGCCCCCGAACACCGAGGCGACCCTGCGCAAAGCCTGCGCCCTTGGCGTCGACGAG
GCGATTCTTCTCTCCGACCGCGCGTTCGGCGGGGCCGACACCCTCTCTACCAGCAACGTTCTCTCGGCGGCCATCGAGAA
GCTGGGAGCCGAGGAAGCGGTGGGGATCGTCTTCTGCGGCAAGCAGACCATCGACGGCGATACCGCCCAGGTGGGACCCG
GCATTGCCACGCGCCTCGGGTACACCCAGCTCACCCTGGTGGACCGGATCGAGTTCCTTGATCTGAAGCGCAATGTGATC
CGGGTGCGGCGCAAGCTTGAAGGGCGGCATGAGATCGTCGAGGCTCCTTTGCCCGTGGTAATTACCGTTGTCCGGGAACT
GAACCGTCCCCGCTATCCAACGGTGCCGATGCGCCTGGCGGCCCAGGAGACCGAGGTCAAGGTCTGGGACAACTCGGTCC
TCAAGCTCGACGTGGAAACGGTGGGCCTGAAGGGTTCCCCCACCTGGGTCAGCAAGATCTTCTCCCCGGAGCGGGACAAG
GGGGAGATCATCGGCGACGGTGTCGGTGATCCGGAGGGGACGGCCAACCTCCTGATCGACAAGCTGCTCAGCAAGGATCT
CTTGGCAGTGTGA

Upstream 100 bases:

>100_bases
GAGCAAAATCCAAGCATAATTTTATCGATTTCTCTGTTGCTCCGTTGCTCAGTGTCATACATGCCGGATCCAGGTTCAAT
AAACCCCAAGGAGAAGTTCT

Downstream 100 bases:

>100_bases
GATGTGAAGGGGGAATGACGATGACTGAAACGAAAAAAACAATCAAGAAGCCCCGCGGCAAGGCCCGCGTTCTCGACGGG
AAATGCATTGCCTGCGGTGC

Product: electron transfer flavoprotein subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLVISCIKQVPDTTQVKIDPVTNTLVREGIPFIVNPYDTHALEEGLNLKDRYGLRSVAISMGPPNTEATLRKACALGVDE
AILLSDRAFGGADTLSTSNVLSAAIEKLGAEEAVGIVFCGKQTIDGDTAQVGPGIATRLGYTQLTLVDRIEFLDLKRNVI
RVRRKLEGRHEIVEAPLPVVITVVRELNRPRYPTVPMRLAAQETEVKVWDNSVLKLDVETVGLKGSPTWVSKIFSPERDK
GEIIGDGVGDPEGTANLLIDKLLSKDLLAV

Sequences:

>Translated_270_residues
MLVISCIKQVPDTTQVKIDPVTNTLVREGIPFIVNPYDTHALEEGLNLKDRYGLRSVAISMGPPNTEATLRKACALGVDE
AILLSDRAFGGADTLSTSNVLSAAIEKLGAEEAVGIVFCGKQTIDGDTAQVGPGIATRLGYTQLTLVDRIEFLDLKRNVI
RVRRKLEGRHEIVEAPLPVVITVVRELNRPRYPTVPMRLAAQETEVKVWDNSVLKLDVETVGLKGSPTWVSKIFSPERDK
GEIIGDGVGDPEGTANLLIDKLLSKDLLAV
>Mature_270_residues
MLVISCIKQVPDTTQVKIDPVTNTLVREGIPFIVNPYDTHALEEGLNLKDRYGLRSVAISMGPPNTEATLRKACALGVDE
AILLSDRAFGGADTLSTSNVLSAAIEKLGAEEAVGIVFCGKQTIDGDTAQVGPGIATRLGYTQLTLVDRIEFLDLKRNVI
RVRRKLEGRHEIVEAPLPVVITVVRELNRPRYPTVPMRLAAQETEVKVWDNSVLKLDVETVGLKGSPTWVSKIFSPERDK
GEIIGDGVGDPEGTANLLIDKLLSKDLLAV

Specific function: May play a role in a redox process involved in nitrogen fixation [H]

COG id: COG2086

COG function: function code C; Electron transfer flavoprotein, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF beta-subunit/fixA family [H]

Homologues:

Organism=Homo sapiens, GI62420877, Length=207, Percent_Identity=30.4347826086957, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4503609, Length=218, Percent_Identity=30.2752293577982, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI87081682, Length=257, Percent_Identity=27.2373540856031, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367123, Length=252, Percent_Identity=24.2063492063492, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25141345, Length=211, Percent_Identity=29.8578199052133, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321646, Length=185, Percent_Identity=32.4324324324324, Blast_Score=99, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24651147, Length=198, Percent_Identity=29.2929292929293, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24651145, Length=198, Percent_Identity=29.2929292929293, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000049
- InterPro:   IPR014730
- InterPro:   IPR012255
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF [H]

EC number: NA

Molecular weight: Translated: 29317; Mature: 29317

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVISCIKQVPDTTQVKIDPVTNTLVREGIPFIVNPYDTHALEEGLNLKDRYGLRSVAIS
CCEEHHHHHCCCCCEEEECCHHHHHHHCCCCEEECCCHHHHHHHCCCCHHHCCCEEEEEE
MGPPNTEATLRKACALGVDEAILLSDRAFGGADTLSTSNVLSAAIEKLGAEEAVGIVFCG
CCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
KQTIDGDTAQVGPGIATRLGYTQLTLVDRIEFLDLKRNVIRVRRKLEGRHEIVEAPLPVV
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHH
ITVVRELNRPRYPTVPMRLAAQETEVKVWDNSVLKLDVETVGLKGSPTWVSKIFSPERDK
HHHHHHHCCCCCCCCCCEEECCCCEEEEECCCEEEEEEEEECCCCCHHHHHHHCCCCCCC
GEIIGDGVGDPEGTANLLIDKLLSKDLLAV
CCEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLVISCIKQVPDTTQVKIDPVTNTLVREGIPFIVNPYDTHALEEGLNLKDRYGLRSVAIS
CCEEHHHHHCCCCCEEEECCHHHHHHHCCCCEEECCCHHHHHHHCCCCHHHCCCEEEEEE
MGPPNTEATLRKACALGVDEAILLSDRAFGGADTLSTSNVLSAAIEKLGAEEAVGIVFCG
CCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
KQTIDGDTAQVGPGIATRLGYTQLTLVDRIEFLDLKRNVIRVRRKLEGRHEIVEAPLPVV
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHH
ITVVRELNRPRYPTVPMRLAAQETEVKVWDNSVLKLDVETVGLKGSPTWVSKIFSPERDK
HHHHHHHCCCCCCCCCCEEECCCCEEEEECCCEEEEEEEEECCCCCHHHHHHHCCCCCCC
GEIIGDGVGDPEGTANLLIDKLLSKDLLAV
CCEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA