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The map label for this gene is rsbU [H]

Identifier: 78223269

GI number: 78223269

Start: 2318631

End: 2319731

Strand: Direct

Name: rsbU [H]

Synonym: Gmet_2062

Alternate gene names: 78223269

Gene position: 2318631-2319731 (Clockwise)

Preceding gene: 78223268

Following gene: 78223280

Centisome position: 58.0

GC content: 59.4

Gene sequence:

>1101_bases
ATGAAGACTCTTACCGTGCAGAGCCAGAAGGGGATGAGCTCCGATCACCTGAATGAATCGGATCGTGAGTTGAGCATGGG
GACATACTCCGGCGAAGCATTCCTCACAAACTCCACCGTAGTGGTCAACGATACGGATTCCATGGACAAGCCCGCCTCCG
CCCTGGTTGTGCGACGGGAAGGAATCAGGTCATTCGCCCATGCCCCCATCACGGTCGAAGGGAAACCCGTGGGTATTCTG
TCGGCCTTCTCAAAGTCGATGAAGGGGATCTTCACCGACGAGTTCGTTGAACTCTTCACCAATCTGGCGGGACAGATCGG
GATAGCGCTTCGCAACGCCCTCCAGGCCGAGAAGCTGATCCAGGCGAAGGAGCATGAAAAGGAGATGCAGATCGCCCGGA
CTATCCAGATGGGGCTCCTGCCGACGCGGGCGCCGGAAGTGGCGGGAATATCACTGGCGGGAACGTGTGTCCCGGCCAGG
GAGGTGGGGGGAGATTACTACGATTTCCTGGTACGCGGCGCCAGGGGTCTCGATCTGGTCATCGCCGACGTATCGGGTCA
CAACGTCGGTGCTGCCCTCCTCATGGCGGAAGTGCGCACCATCATCCAGGCAGAGGCGAAGATCGTCCGGGGTCCCGGCG
AGATACTGTGCGTCCTCAACGACTTTCTCTACGAAGACCTCACCCGTGCCGAACTCTTCATCACCATGTTCTACCTGAAC
TACGATGCCGGGACGCGGCTGCTCTCCTTCGCCAGCGCCGGCCACAACCCGCCGATACTCAGGAGGTCCGGATCACGGGC
CTGTGAGTACCTCGACGCGGAGGGCCTCATTCTCGGCATCAAGAGGAACGTCACGTTTGAGGAAAAACAGGTGCGGCTGG
AGCCGGGAGATCTCCTCCTGCTCTACACCGATGGCATCACCGAGGCGGCAAATCCCGCCGGCGAATTATTCGGCGAGGAC
CGGCTATGCTCCCTTATTGCCGAGTATCACGCACTCCATCCCCAGCAGATTATCGACAATCTCCTCGCCCAGGTCAGATC
GTTCGCCGCAAACCAGAACCTGACCGACGATGTCTCGCTGGTCGTTCTGAAGGCACTCTAA

Upstream 100 bases:

>100_bases
GGTTAGTGGCGACCTGAAGACCATCCTGGACAGCATCCTGGAGCTTCTCCAGCAGCAGTTCAAGTTCGACACGTGCGTTA
TCCGCATTCTCGACGAAGAA

Downstream 100 bases:

>100_bases
GAGGCCGTGGCGGGAATGACACCCTGATCGCAGAGCACCTGATAGGCGAGGATCCGACTCCGCATGTCGTCAGGAAAGGG
GATCGCCTTGATGGAGCCGG

Product: serine phosphatase

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKTLTVQSQKGMSSDHLNESDRELSMGTYSGEAFLTNSTVVVNDTDSMDKPASALVVRREGIRSFAHAPITVEGKPVGIL
SAFSKSMKGIFTDEFVELFTNLAGQIGIALRNALQAEKLIQAKEHEKEMQIARTIQMGLLPTRAPEVAGISLAGTCVPAR
EVGGDYYDFLVRGARGLDLVIADVSGHNVGAALLMAEVRTIIQAEAKIVRGPGEILCVLNDFLYEDLTRAELFITMFYLN
YDAGTRLLSFASAGHNPPILRRSGSRACEYLDAEGLILGIKRNVTFEEKQVRLEPGDLLLLYTDGITEAANPAGELFGED
RLCSLIAEYHALHPQQIIDNLLAQVRSFAANQNLTDDVSLVVLKAL

Sequences:

>Translated_366_residues
MKTLTVQSQKGMSSDHLNESDRELSMGTYSGEAFLTNSTVVVNDTDSMDKPASALVVRREGIRSFAHAPITVEGKPVGIL
SAFSKSMKGIFTDEFVELFTNLAGQIGIALRNALQAEKLIQAKEHEKEMQIARTIQMGLLPTRAPEVAGISLAGTCVPAR
EVGGDYYDFLVRGARGLDLVIADVSGHNVGAALLMAEVRTIIQAEAKIVRGPGEILCVLNDFLYEDLTRAELFITMFYLN
YDAGTRLLSFASAGHNPPILRRSGSRACEYLDAEGLILGIKRNVTFEEKQVRLEPGDLLLLYTDGITEAANPAGELFGED
RLCSLIAEYHALHPQQIIDNLLAQVRSFAANQNLTDDVSLVVLKAL
>Mature_366_residues
MKTLTVQSQKGMSSDHLNESDRELSMGTYSGEAFLTNSTVVVNDTDSMDKPASALVVRREGIRSFAHAPITVEGKPVGIL
SAFSKSMKGIFTDEFVELFTNLAGQIGIALRNALQAEKLIQAKEHEKEMQIARTIQMGLLPTRAPEVAGISLAGTCVPAR
EVGGDYYDFLVRGARGLDLVIADVSGHNVGAALLMAEVRTIIQAEAKIVRGPGEILCVLNDFLYEDLTRAELFITMFYLN
YDAGTRLLSFASAGHNPPILRRSGSRACEYLDAEGLILGIKRNVTFEEKQVRLEPGDLLLLYTDGITEAANPAGELFGED
RLCSLIAEYHALHPQQIIDNLLAQVRSFAANQNLTDDVSLVVLKAL

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 39843; Mature: 39843

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTVQSQKGMSSDHLNESDRELSMGTYSGEAFLTNSTVVVNDTDSMDKPASALVVRRE
CCEEEECCCCCCCCCCCCCCCCEEECCEECCCEEEECCEEEEECCCCCCCCHHHHHHHHH
GIRSFAHAPITVEGKPVGILSAFSKSMKGIFTDEFVELFTNLAGQIGIALRNALQAEKLI
HHHHHHCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKEHEKEMQIARTIQMGLLPTRAPEVAGISLAGTCVPAREVGGDYYDFLVRGARGLDLV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHCCHHHHHHHCCCCCCEEE
IADVSGHNVGAALLMAEVRTIIQAEAKIVRGPGEILCVLNDFLYEDLTRAELFITMFYLN
EEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHEEEEEEEEE
YDAGTRLLSFASAGHNPPILRRSGSRACEYLDAEGLILGIKRNVTFEEKQVRLEPGDLLL
CCCCHHHHHHHHCCCCCCCEECCCCHHHHHCCCCCEEEEEECCCEECCCEEEECCCCEEE
LYTDGITEAANPAGELFGEDRLCSLIAEYHALHPQQIIDNLLAQVRSFAANQNLTDDVSL
EEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHE
VVLKAL
EEEECC
>Mature Secondary Structure
MKTLTVQSQKGMSSDHLNESDRELSMGTYSGEAFLTNSTVVVNDTDSMDKPASALVVRRE
CCEEEECCCCCCCCCCCCCCCCEEECCEECCCEEEECCEEEEECCCCCCCCHHHHHHHHH
GIRSFAHAPITVEGKPVGILSAFSKSMKGIFTDEFVELFTNLAGQIGIALRNALQAEKLI
HHHHHHCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKEHEKEMQIARTIQMGLLPTRAPEVAGISLAGTCVPAREVGGDYYDFLVRGARGLDLV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHCCHHHHHHHCCCCCCEEE
IADVSGHNVGAALLMAEVRTIIQAEAKIVRGPGEILCVLNDFLYEDLTRAELFITMFYLN
EEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHHHEEEEEEEEE
YDAGTRLLSFASAGHNPPILRRSGSRACEYLDAEGLILGIKRNVTFEEKQVRLEPGDLLL
CCCCHHHHHHHHCCCCCCCEECCCCHHHHHCCCCCEEEEEECCCEECCCEEEECCCCEEE
LYTDGITEAANPAGELFGEDRLCSLIAEYHALHPQQIIDNLLAQVRSFAANQNLTDDVSL
EEECCCCCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHE
VVLKAL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]