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The map label for this gene is fusA

Identifier: 78223192

GI number: 78223192

Start: 2213849

End: 2215942

Strand: Reverse

Name: fusA

Synonym: Gmet_1985

Alternate gene names: 78223192

Gene position: 2215942-2213849 (Counterclockwise)

Preceding gene: 78223193

Following gene: 78223191

Centisome position: 55.43

GC content: 56.78

Gene sequence:

>2094_bases
ATGGGCAAGTATGATACCGCAAAGCTGAGAAACCTCGGAATTGTCGCCCACGGCGGCGCAGGCAAGACCTCTCTCACCGA
AGCCATACTCTTCACCACGGGGATGATTGACCGTATCGGCCGGGTCGACGACGGCACGTCCACCATGGATTTCGAGCCCG
AGGAGATCAAGCGGAAGATCACAATCTCATCCTCCCTCGACCACTGTGAATGGAATGACTACTCGCTTCACATTGTCGAT
ACCCCCGGTTACGGAAACTTTATCTCAGACACCCGCGCCTGCATGCGTGCCTTGGGCGGATGCATCGTTATCCTTTCTGC
CATCTCCGGGGTAAAGGTCCAGACCGAGGAGGTCTGGGAGTGGGCCAACGAGTTCGAGCTGCCCCGCATCGCCTTCGTCA
ACAAGATGGACCGCGAATACGCCAACTTCCTCCGGGCCCTCGACGACATGGAGAAGTCTCTCAAGGCCCGTGGCGTGGCG
GTCCAGATGCCCTTGGGCGCAGCTGAAACCTTCGAGGGGATCATTGATCTCATCGACATGAAGGCATACCGCTACGCCAA
GGATGGTTCCGGAACATTCACGGAGGAGGAGATTCCCGCCGGGGAGCGGGACGAGGCCCAGCGACTCCGCGATATCCTCG
TGGAAACCGTTGCAGAGGCCTACGATGCCCTGACCGAGAAGTACCTGGAAACCGGCGAACTTTCCCGGGAAGAGATTCTG
GACGGCCTGCGGATCGGGACGCTCCGCTATACCTTTACGCCGGTTTTTTGCGGTTCCGCCACCATGAACATCGGCGTTCA
TCACCTCCTTGATTACATCTGCCACTGCCTCCCCTCTCCCCTCGACCGGGGTGTGGTGGTCGGCACCAATCCCAAAAACG
GTGAGATGGAGGAACGCAAGCCCGATGAAACGGAACCCTTCTCTGCCCTCGTCTTCAAGACCACGTCGGATCCCTACACC
GGCAAAATATCCATCTTCCGGGTCTACTCGGGTACCCTGAACTCCGACTCGACCATCTACAACCCGGTCCGCGACTGCGA
GGAGCGGATCGGCCAGATCTACGAACTGGAGGGGAAGAAGCAGAAGTCTATCAAACAGGCCGTTGCCGGCGACATCGTGG
CCGTGGCCAAGCTTAAGGAAACCCTTACCGGCGATACCCTCTGCGACAAGGACAAGCCGATCATCTTCGAGCCGGCCAAG
CCGCTCCAACCGGTCATTTCCTACGCTATCCAGCCCAAGACGAAAAATGATGAAGACAAGATTCACGGTGCTCTCCAGAA
GCTCATGGAAGAGGATCAGACGATTCAGGCGCGACGTGACGAAAAGACCCATGAACTGATTCTCTCGGGAATGGGGCAGG
TACACCTTGAGGTCACCATCGAGAAGCTGAAGCGCAAGTTCAATGTCGATGTGGAAATGAAGACCCAGAAGGTGCCGTAC
CTGGAAACCTTCAAAGCCACTGTCAAGGCCCAGGGAAAATACAAGAAGCAGTCGGGGGGGAGAGGACAGTACGGCGACTG
CTGGGTTGAGTTTGCTCCCCAGCAGCGTGGTGAGGGATTTCAGTTTGAGGACAAGATCGTCGGTGGCGTCATCCCGCGCC
AGTACATCCCGGCGGTTGAGAAGGGGATCTTCGAGGCTTCCCAAGACGGTTTCCTGGCCGGTTATCCTTTGGTGGATTTC
AAGGCCGCCGTCTATGACGGGTCCTTCCACACCGTGGATTCATCCGAAATGGCCTTCAAGGTTGCCGGTTCCCTGGCGTT
CAAAAAGGCCATGGAAACGGCCAAGGTGATACTCCTTGAGCCGATGATGAACATGAAGATCACGGTTCCCGAAGAGACCA
TGGGTGACGTGATCGGAGACCTAAACTCCCGACGCGGCAAAGTGGTCGGCGTCGAACCCAAGGCCAATTCACAGATCATC
AGGTCAGTGGTTCCCATGGCCGAGGTGCTTTCCTACGCCAACGACCTGAAATCCATGACCAGTGACCGGGGGCTTTTCAC
CATGGAATTTTCCCACTACGAAGAGGTGCCAAGTCATTTGGCCCAGAAGATCATCGCCGAATCGGCCAAGGTCAATGAGA
AGAATAACCACTGA

Upstream 100 bases:

>100_bases
GACTGCGGATACTGTACGAACGAAACAGGGAGTGAGATGACGAGATAGCGCGGAAGTCAAAAGGTCGCCAGACCTATACC
TTATCATCACGGGAGGATGT

Downstream 100 bases:

>100_bases
CCACGGCATTAACAGGAGGAACGCATCAGATGAACATCGAATTTGATCAGGAACCTGGAGATACATCATCAAAGGGTGGG
GGGAGCAATACCCGTCTCCT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MGKYDTAKLRNLGIVAHGGAGKTSLTEAILFTTGMIDRIGRVDDGTSTMDFEPEEIKRKITISSSLDHCEWNDYSLHIVD
TPGYGNFISDTRACMRALGGCIVILSAISGVKVQTEEVWEWANEFELPRIAFVNKMDREYANFLRALDDMEKSLKARGVA
VQMPLGAAETFEGIIDLIDMKAYRYAKDGSGTFTEEEIPAGERDEAQRLRDILVETVAEAYDALTEKYLETGELSREEIL
DGLRIGTLRYTFTPVFCGSATMNIGVHHLLDYICHCLPSPLDRGVVVGTNPKNGEMEERKPDETEPFSALVFKTTSDPYT
GKISIFRVYSGTLNSDSTIYNPVRDCEERIGQIYELEGKKQKSIKQAVAGDIVAVAKLKETLTGDTLCDKDKPIIFEPAK
PLQPVISYAIQPKTKNDEDKIHGALQKLMEEDQTIQARRDEKTHELILSGMGQVHLEVTIEKLKRKFNVDVEMKTQKVPY
LETFKATVKAQGKYKKQSGGRGQYGDCWVEFAPQQRGEGFQFEDKIVGGVIPRQYIPAVEKGIFEASQDGFLAGYPLVDF
KAAVYDGSFHTVDSSEMAFKVAGSLAFKKAMETAKVILLEPMMNMKITVPEETMGDVIGDLNSRRGKVVGVEPKANSQII
RSVVPMAEVLSYANDLKSMTSDRGLFTMEFSHYEEVPSHLAQKIIAESAKVNEKNNH

Sequences:

>Translated_697_residues
MGKYDTAKLRNLGIVAHGGAGKTSLTEAILFTTGMIDRIGRVDDGTSTMDFEPEEIKRKITISSSLDHCEWNDYSLHIVD
TPGYGNFISDTRACMRALGGCIVILSAISGVKVQTEEVWEWANEFELPRIAFVNKMDREYANFLRALDDMEKSLKARGVA
VQMPLGAAETFEGIIDLIDMKAYRYAKDGSGTFTEEEIPAGERDEAQRLRDILVETVAEAYDALTEKYLETGELSREEIL
DGLRIGTLRYTFTPVFCGSATMNIGVHHLLDYICHCLPSPLDRGVVVGTNPKNGEMEERKPDETEPFSALVFKTTSDPYT
GKISIFRVYSGTLNSDSTIYNPVRDCEERIGQIYELEGKKQKSIKQAVAGDIVAVAKLKETLTGDTLCDKDKPIIFEPAK
PLQPVISYAIQPKTKNDEDKIHGALQKLMEEDQTIQARRDEKTHELILSGMGQVHLEVTIEKLKRKFNVDVEMKTQKVPY
LETFKATVKAQGKYKKQSGGRGQYGDCWVEFAPQQRGEGFQFEDKIVGGVIPRQYIPAVEKGIFEASQDGFLAGYPLVDF
KAAVYDGSFHTVDSSEMAFKVAGSLAFKKAMETAKVILLEPMMNMKITVPEETMGDVIGDLNSRRGKVVGVEPKANSQII
RSVVPMAEVLSYANDLKSMTSDRGLFTMEFSHYEEVPSHLAQKIIAESAKVNEKNNH
>Mature_696_residues
GKYDTAKLRNLGIVAHGGAGKTSLTEAILFTTGMIDRIGRVDDGTSTMDFEPEEIKRKITISSSLDHCEWNDYSLHIVDT
PGYGNFISDTRACMRALGGCIVILSAISGVKVQTEEVWEWANEFELPRIAFVNKMDREYANFLRALDDMEKSLKARGVAV
QMPLGAAETFEGIIDLIDMKAYRYAKDGSGTFTEEEIPAGERDEAQRLRDILVETVAEAYDALTEKYLETGELSREEILD
GLRIGTLRYTFTPVFCGSATMNIGVHHLLDYICHCLPSPLDRGVVVGTNPKNGEMEERKPDETEPFSALVFKTTSDPYTG
KISIFRVYSGTLNSDSTIYNPVRDCEERIGQIYELEGKKQKSIKQAVAGDIVAVAKLKETLTGDTLCDKDKPIIFEPAKP
LQPVISYAIQPKTKNDEDKIHGALQKLMEEDQTIQARRDEKTHELILSGMGQVHLEVTIEKLKRKFNVDVEMKTQKVPYL
ETFKATVKAQGKYKKQSGGRGQYGDCWVEFAPQQRGEGFQFEDKIVGGVIPRQYIPAVEKGIFEASQDGFLAGYPLVDFK
AAVYDGSFHTVDSSEMAFKVAGSLAFKKAMETAKVILLEPMMNMKITVPEETMGDVIGDLNSRRGKVVGVEPKANSQIIR
SVVPMAEVLSYANDLKSMTSDRGLFTMEFSHYEEVPSHLAQKIIAESAKVNEKNNH

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=679, Percent_Identity=34.1678939617084, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI19923640, Length=708, Percent_Identity=29.5197740112994, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI25306287, Length=708, Percent_Identity=27.5423728813559, Blast_Score=279, Evalue=6e-75,
Organism=Homo sapiens, GI25306283, Length=392, Percent_Identity=34.4387755102041, Blast_Score=212, Evalue=8e-55,
Organism=Homo sapiens, GI4503483, Length=858, Percent_Identity=20.6293706293706, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157426893, Length=156, Percent_Identity=30.1282051282051, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI94966754, Length=138, Percent_Identity=31.8840579710145, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789738, Length=697, Percent_Identity=43.7589670014347, Blast_Score=580, Evalue=1e-166,
Organism=Escherichia coli, GI1790835, Length=485, Percent_Identity=25.979381443299, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994988, Length=173, Percent_Identity=32.3699421965318, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=696, Percent_Identity=31.8965517241379, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17556745, Length=720, Percent_Identity=24.5833333333333, Blast_Score=229, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6323098, Length=699, Percent_Identity=32.4749642346209, Blast_Score=385, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6322359, Length=792, Percent_Identity=27.3989898989899, Blast_Score=282, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6323320, Length=147, Percent_Identity=29.2517006802721, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324707, Length=167, Percent_Identity=26.9461077844311, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=167, Percent_Identity=26.9461077844311, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24582462, Length=697, Percent_Identity=33.142037302726, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI221458488, Length=728, Percent_Identity=26.2362637362637, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24585709, Length=813, Percent_Identity=21.8942189421894, Blast_Score=109, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24585711, Length=813, Percent_Identity=21.8942189421894, Blast_Score=109, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24585713, Length=813, Percent_Identity=21.8942189421894, Blast_Score=109, Evalue=8e-24,
Organism=Drosophila melanogaster, GI78706572, Length=177, Percent_Identity=25.9887005649718, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77621; Mature: 77490

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKYDTAKLRNLGIVAHGGAGKTSLTEAILFTTGMIDRIGRVDDGTSTMDFEPEEIKRKI
CCCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHEE
TISSSLDHCEWNDYSLHIVDTPGYGNFISDTRACMRALGGCIVILSAISGVKVQTEEVWE
EECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
WANEFELPRIAFVNKMDREYANFLRALDDMEKSLKARGVAVQMPLGAAETFEGIIDLIDM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
KAYRYAKDGSGTFTEEEIPAGERDEAQRLRDILVETVAEAYDALTEKYLETGELSREEIL
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DGLRIGTLRYTFTPVFCGSATMNIGVHHLLDYICHCLPSPLDRGVVVGTNPKNGEMEERK
CCCEECEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCC
PDETEPFSALVFKTTSDPYTGKISIFRVYSGTLNSDSTIYNPVRDCEERIGQIYELEGKK
CCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEECCCCH
QKSIKQAVAGDIVAVAKLKETLTGDTLCDKDKPIIFEPAKPLQPVISYAIQPKTKNDEDK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCHHH
IHGALQKLMEEDQTIQARRDEKTHELILSGMGQVHLEVTIEKLKRKFNVDVEMKTQKVPY
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEEECCCCCH
LETFKATVKAQGKYKKQSGGRGQYGDCWVEFAPQQRGEGFQFEDKIVGGVIPRQYIPAVE
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHCCCCCCCHHHHHCCCCCHHHHHHHH
KGIFEASQDGFLAGYPLVDFKAAVYDGSFHTVDSSEMAFKVAGSLAFKKAMETAKVILLE
HHHHHCCCCCCEECCCHHHCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEE
PMMNMKITVPEETMGDVIGDLNSRRGKVVGVEPKANSQIIRSVVPMAEVLSYANDLKSMT
CCCCCEEECCHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SDRGLFTMEFSHYEEVPSHLAQKIIAESAKVNEKNNH
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GKYDTAKLRNLGIVAHGGAGKTSLTEAILFTTGMIDRIGRVDDGTSTMDFEPEEIKRKI
CCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHEE
TISSSLDHCEWNDYSLHIVDTPGYGNFISDTRACMRALGGCIVILSAISGVKVQTEEVWE
EECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
WANEFELPRIAFVNKMDREYANFLRALDDMEKSLKARGVAVQMPLGAAETFEGIIDLIDM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHH
KAYRYAKDGSGTFTEEEIPAGERDEAQRLRDILVETVAEAYDALTEKYLETGELSREEIL
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DGLRIGTLRYTFTPVFCGSATMNIGVHHLLDYICHCLPSPLDRGVVVGTNPKNGEMEERK
CCCEECEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCC
PDETEPFSALVFKTTSDPYTGKISIFRVYSGTLNSDSTIYNPVRDCEERIGQIYELEGKK
CCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEECCCCH
QKSIKQAVAGDIVAVAKLKETLTGDTLCDKDKPIIFEPAKPLQPVISYAIQPKTKNDEDK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHCCCCCCCCHHH
IHGALQKLMEEDQTIQARRDEKTHELILSGMGQVHLEVTIEKLKRKFNVDVEMKTQKVPY
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEEECCCCCH
LETFKATVKAQGKYKKQSGGRGQYGDCWVEFAPQQRGEGFQFEDKIVGGVIPRQYIPAVE
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHCCCCCCCHHHHHCCCCCHHHHHHHH
KGIFEASQDGFLAGYPLVDFKAAVYDGSFHTVDSSEMAFKVAGSLAFKKAMETAKVILLE
HHHHHCCCCCCEECCCHHHCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEE
PMMNMKITVPEETMGDVIGDLNSRRGKVVGVEPKANSQIIRSVVPMAEVLSYANDLKSMT
CCCCCEEECCHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SDRGLFTMEFSHYEEVPSHLAQKIIAESAKVNEKNNH
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA