Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 78223180

GI number: 78223180

Start: 2203609

End: 2204265

Strand: Reverse

Name: nth [H]

Synonym: Gmet_1973

Alternate gene names: 78223180

Gene position: 2204265-2203609 (Counterclockwise)

Preceding gene: 78223181

Following gene: 78223179

Centisome position: 55.14

GC content: 54.79

Gene sequence:

>657_bases
GTGAAAGACAGCGATATCCATGAGGCCATGGCTCTCCTGGCGGAGGCTGTGAAATCGTGGCCGTCCCCCGCAGTTACCAT
CGTCTCGCAGCGGGAGGGAAATCCCTTCAAGGTCCTAGTCTCCTGCATCCTTTCTCTTCGTACCCAGGACAGGACCACCG
GTCCGGCATCGGAACGACTCTTTGGCCTGGCCGATACCCCGGCGAAGATGCTTTTTCTCTCCTCGGATGCGATTGAACAG
GCCATTTACCCTGTGGGATTTTATCGAAACAAGGCAGCCCAGATTCTCGATATCTGCAGAACCCTCGTGGATAAATACGA
CGGACAGGTTCCCGATGATCTGGATGAACTGCTGACTTTCAGGGGGGTAGGGCGCAAGACAGCGAACCTGGTTCTGACTC
TTGGTTTCGGTAAACTGGCCATTTGTGTCGATACCCATGTCCACCGCATTTGTAACCGGTGGGGATACACCAGCACGAAG
ACGCCGGCGGAGACGGAGTTTGCGCTTCGCGCCAAGCTTCCCCAAAAGTACTGGCCCGTAATCAACGATTACCTCGTCAC
TTTTGGCCAGAACCAGTGTACGCCTGTTTCGCCCCGTTGCTCCACCTGTGTGCTTGTTTGTTTCTGTGACAGAGTAGGCG
TGACAAAATCGCGATAA

Upstream 100 bases:

>100_bases
GCGAAGGGCGGAGTCTCGGTTATCTTTTCTTCTGCGTGGGTATCAGCCTCTCCGTCATGGGGGTCCTCTTTATGCGCATT
TTCAGGAACCGGGGCCTGTC

Downstream 100 bases:

>100_bases
AGCAAACGGTGTTATTTTTTTAAACTCTTCTATTGTACAAGAGACAAACACATTGTACTATTAACACCGTTCGGAGATGT
GATTACTTACTGTAAATTCA

Product: HhH-GPD

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKDSDIHEAMALLAEAVKSWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERLFGLADTPAKMLFLSSDAIEQ
AIYPVGFYRNKAAQILDICRTLVDKYDGQVPDDLDELLTFRGVGRKTANLVLTLGFGKLAICVDTHVHRICNRWGYTSTK
TPAETEFALRAKLPQKYWPVINDYLVTFGQNQCTPVSPRCSTCVLVCFCDRVGVTKSR

Sequences:

>Translated_218_residues
MKDSDIHEAMALLAEAVKSWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERLFGLADTPAKMLFLSSDAIEQ
AIYPVGFYRNKAAQILDICRTLVDKYDGQVPDDLDELLTFRGVGRKTANLVLTLGFGKLAICVDTHVHRICNRWGYTSTK
TPAETEFALRAKLPQKYWPVINDYLVTFGQNQCTPVSPRCSTCVLVCFCDRVGVTKSR
>Mature_218_residues
MKDSDIHEAMALLAEAVKSWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERLFGLADTPAKMLFLSSDAIEQ
AIYPVGFYRNKAAQILDICRTLVDKYDGQVPDDLDELLTFRGVGRKTANLVLTLGFGKLAICVDTHVHRICNRWGYTSTK
TPAETEFALRAKLPQKYWPVINDYLVTFGQNQCTPVSPRCSTCVLVCFCDRVGVTKSR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=40.6779661016949, Blast_Score=135, Evalue=2e-32,
Organism=Escherichia coli, GI1787920, Length=178, Percent_Identity=34.8314606741573, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789331, Length=190, Percent_Identity=25.7894736842105, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=170, Percent_Identity=43.5294117647059, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319304, Length=168, Percent_Identity=34.5238095238095, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=188, Percent_Identity=32.4468085106383, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=38.4180790960452, Blast_Score=129, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24207; Mature: 24207

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSDIHEAMALLAEAVKSWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERL
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHE
FGLADTPAKMLFLSSDAIEQAIYPVGFYRNKAAQILDICRTLVDKYDGQVPDDLDELLTF
ECCCCCCHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RGVGRKTANLVLTLGFGKLAICVDTHVHRICNRWGYTSTKTPAETEFALRAKLPQKYWPV
HCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHH
INDYLVTFGQNQCTPVSPRCSTCVLVCFCDRVGVTKSR
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKDSDIHEAMALLAEAVKSWPSPAVTIVSQREGNPFKVLVSCILSLRTQDRTTGPASERL
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHE
FGLADTPAKMLFLSSDAIEQAIYPVGFYRNKAAQILDICRTLVDKYDGQVPDDLDELLTF
ECCCCCCHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RGVGRKTANLVLTLGFGKLAICVDTHVHRICNRWGYTSTKTPAETEFALRAKLPQKYWPV
HCCCCCCEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHH
INDYLVTFGQNQCTPVSPRCSTCVLVCFCDRVGVTKSR
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]