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The map label for this gene is lpdA [H]

Identifier: 78223103

GI number: 78223103

Start: 2116956

End: 2118314

Strand: Direct

Name: lpdA [H]

Synonym: Gmet_1896

Alternate gene names: 78223103

Gene position: 2116956-2118314 (Clockwise)

Preceding gene: 78223102

Following gene: 78223104

Centisome position: 52.96

GC content: 56.51

Gene sequence:

>1359_bases
GTGAAAAGCTTTGATGTGGTCATTATCGGCGGTGGCCCTGGCGGAATCAATGCGGGAATAATGCTCAGCAAGGCGGGCAA
AAATGTTGCCCTGATTCAGGAAGAGGCGGTCTCTTTCGGCGGCACCTGCCTGAATAGCGGCTGTATGCCGACAAAGTCGC
TGTTAAAGGCCGCGACGGCTTACCGGTACGCCAAACAGGCAGAAAGATATGGTTTGGACCTGCAGGCGGGACCCGTCGAT
TTAGGGAAACTGTGCGCCGTTACTGAAACAGATCTCAACATGCTGCGTGGGGCGATCAAGGGAATGCTCGCGGAAACCTC
GATTACGGCATTCCGTGGGAACGGCTCTTTCGCGTCGGAGCATGAAGTCACGGTCACCATGGCCGGTGGCGAGCAGGAGA
CGATCTGGGGCGAAACGATCATTATTGCAACGGGTTCGAGCCCCCGGGAACTCCCCTCGGCGCCCTTCGACGGTTTGCAC
ATCCTTTCGAGCGACCAGATGCTGACCAACACCGACCTGCCGAAAAAACTCCTGATCGTCGGTGGAGGTGCGATCGGATG
TGAGTTCGCCACCTTGTATAAAACGTTTGGCAGCGAGGTCGTACTCATCGAGGCGATGGAAACCCTTCTGCCGCGCGAAG
ACCGCGACGCCGGCAAAAAACTGCAGGCAGCCTTTGAAGCTCAAGGGATTACCGTCAGGACCGGCACGTCGATCGACCGG
CTCATCGTAGTAGATGAAAAAATCAGGATACAATTTGACAATGGCGACAGCATCGATAGCATCGACAAGGTTCTGGTCGG
AATCGGCAGGGCTCCCAACATCGAGGGGCTGAACCTGGAAGCGGCCGGCCTGCGAACTGAGCATGGCGCCGTCAAGGTGA
ATGAATTAATGCAAACCAATGTGCCGCACATCTACGCCATCGGAGACGTGACCGGTGGGCTGACGCTGGCCCATGCGGCA
CAAAGGGAGGCGCAACTGCTGGTCCAAAATCTGCTGCATGGGAGCAGCCATGCTCTCAAAGAACTGGCTGTCCCCCGGGT
GGCTTTCAGCTATCCGGAGGTGGCAGCAGTGGGGGCATCCCAGGAAGGAAACGGCATCAGGGCCTACACCTTACCCCAGG
TTCCCAATGGACGTTCAGTGGTGGACAAGGTTGCCCCGGCCTTCGTGAAGCTTTTTTTGAAGGAGCAAACCTCTGAAATC
GCCGGAGCGGTAATCATCGGAGAGGCGGCGACAGAAATCATACACGAGATGGCATTGGCGGTGGAGAACGGCCTTACCCT
GCAACAGGTGGGTAATACGGTTCATGTCCACCCGACCCATTCTAAAAACATCCTCTATGCGATACAAAGCTGCGGTTAA

Upstream 100 bases:

>100_bases
TGTCGGTTGAAGAGCGGGACACTTTCGAACGGTTAGTCGATATGGCGGTGAAGGGACTTGATGACCGGCACCAACAGACT
GAACGAAAAAAGGAGGATGT

Downstream 100 bases:

>100_bases
AACTCCGCAGCCATTCACCGCCTCGGTGTGAAGGCCGGAAAATACGTAAGGGAAGCAATCTTCCGGACAAATCCTTTTTC
GGAGACAACCACAAGAGCAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKSFDVVIIGGGPGGINAGIMLSKAGKNVALIQEEAVSFGGTCLNSGCMPTKSLLKAATAYRYAKQAERYGLDLQAGPVD
LGKLCAVTETDLNMLRGAIKGMLAETSITAFRGNGSFASEHEVTVTMAGGEQETIWGETIIIATGSSPRELPSAPFDGLH
ILSSDQMLTNTDLPKKLLIVGGGAIGCEFATLYKTFGSEVVLIEAMETLLPREDRDAGKKLQAAFEAQGITVRTGTSIDR
LIVVDEKIRIQFDNGDSIDSIDKVLVGIGRAPNIEGLNLEAAGLRTEHGAVKVNELMQTNVPHIYAIGDVTGGLTLAHAA
QREAQLLVQNLLHGSSHALKELAVPRVAFSYPEVAAVGASQEGNGIRAYTLPQVPNGRSVVDKVAPAFVKLFLKEQTSEI
AGAVIIGEAATEIIHEMALAVENGLTLQQVGNTVHVHPTHSKNILYAIQSCG

Sequences:

>Translated_452_residues
MKSFDVVIIGGGPGGINAGIMLSKAGKNVALIQEEAVSFGGTCLNSGCMPTKSLLKAATAYRYAKQAERYGLDLQAGPVD
LGKLCAVTETDLNMLRGAIKGMLAETSITAFRGNGSFASEHEVTVTMAGGEQETIWGETIIIATGSSPRELPSAPFDGLH
ILSSDQMLTNTDLPKKLLIVGGGAIGCEFATLYKTFGSEVVLIEAMETLLPREDRDAGKKLQAAFEAQGITVRTGTSIDR
LIVVDEKIRIQFDNGDSIDSIDKVLVGIGRAPNIEGLNLEAAGLRTEHGAVKVNELMQTNVPHIYAIGDVTGGLTLAHAA
QREAQLLVQNLLHGSSHALKELAVPRVAFSYPEVAAVGASQEGNGIRAYTLPQVPNGRSVVDKVAPAFVKLFLKEQTSEI
AGAVIIGEAATEIIHEMALAVENGLTLQQVGNTVHVHPTHSKNILYAIQSCG
>Mature_452_residues
MKSFDVVIIGGGPGGINAGIMLSKAGKNVALIQEEAVSFGGTCLNSGCMPTKSLLKAATAYRYAKQAERYGLDLQAGPVD
LGKLCAVTETDLNMLRGAIKGMLAETSITAFRGNGSFASEHEVTVTMAGGEQETIWGETIIIATGSSPRELPSAPFDGLH
ILSSDQMLTNTDLPKKLLIVGGGAIGCEFATLYKTFGSEVVLIEAMETLLPREDRDAGKKLQAAFEAQGITVRTGTSIDR
LIVVDEKIRIQFDNGDSIDSIDKVLVGIGRAPNIEGLNLEAAGLRTEHGAVKVNELMQTNVPHIYAIGDVTGGLTLAHAA
QREAQLLVQNLLHGSSHALKELAVPRVAFSYPEVAAVGASQEGNGIRAYTLPQVPNGRSVVDKVAPAFVKLFLKEQTSEI
AGAVIIGEAATEIIHEMALAVENGLTLQQVGNTVHVHPTHSKNILYAIQSCG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=452, Percent_Identity=31.4159292035398, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI22035672, Length=450, Percent_Identity=28.4444444444444, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=25.1612903225806, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI33519430, Length=434, Percent_Identity=26.9585253456221, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33519428, Length=434, Percent_Identity=26.9585253456221, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33519426, Length=434, Percent_Identity=26.9585253456221, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI148277065, Length=434, Percent_Identity=26.9585253456221, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI148277071, Length=434, Percent_Identity=26.9585253456221, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI291045266, Length=431, Percent_Identity=24.8259860788863, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI291045268, Length=424, Percent_Identity=23.8207547169811, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=29.4642857142857, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI87082354, Length=451, Percent_Identity=29.0465631929047, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=28.6026200873362, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=27.0642201834862, Blast_Score=135, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=451, Percent_Identity=32.8159645232816, Blast_Score=207, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI17557007, Length=469, Percent_Identity=25.7995735607676, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71983419, Length=432, Percent_Identity=26.1574074074074, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=432, Percent_Identity=26.1574074074074, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=478, Percent_Identity=23.2217573221757, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=461, Percent_Identity=32.3210412147505, Blast_Score=209, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=28.0590717299578, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=26.7094017094017, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21358499, Length=451, Percent_Identity=34.1463414634146, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI17737741, Length=470, Percent_Identity=27.4468085106383, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640553, Length=469, Percent_Identity=27.5053304904051, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24640549, Length=469, Percent_Identity=27.5053304904051, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24640551, Length=463, Percent_Identity=27.8617710583153, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47660; Mature: 47660

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFDVVIIGGGPGGINAGIMLSKAGKNVALIQEEAVSFGGTCLNSGCMPTKSLLKAATA
CCCEEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHH
YRYAKQAERYGLDLQAGPVDLGKLCAVTETDLNMLRGAIKGMLAETSITAFRGNGSFASE
HHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCC
HEVTVTMAGGEQETIWGETIIIATGSSPRELPSAPFDGLHILSSDQMLTNTDLPKKLLIV
CEEEEEEECCCCCEECCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEECCCCCCEEEEE
GGGAIGCEFATLYKTFGSEVVLIEAMETLLPREDRDAGKKLQAAFEAQGITVRTGTSIDR
ECCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCE
LIVVDEKIRIQFDNGDSIDSIDKVLVGIGRAPNIEGLNLEAAGLRTEHGAVKVNELMQTN
EEEEECEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEHHHHHHCC
VPHIYAIGDVTGGLTLAHAAQREAQLLVQNLLHGSSHALKELAVPRVAFSYPEVAAVGAS
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHEECCCCCEEECCC
QEGNGIRAYTLPQVPNGRSVVDKVAPAFVKLFLKEQTSEIAGAVIIGEAATEIIHEMALA
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHH
VENGLTLQQVGNTVHVHPTHSKNILYAIQSCG
HHCCCCHHHCCCEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure
MKSFDVVIIGGGPGGINAGIMLSKAGKNVALIQEEAVSFGGTCLNSGCMPTKSLLKAATA
CCCEEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHH
YRYAKQAERYGLDLQAGPVDLGKLCAVTETDLNMLRGAIKGMLAETSITAFRGNGSFASE
HHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCC
HEVTVTMAGGEQETIWGETIIIATGSSPRELPSAPFDGLHILSSDQMLTNTDLPKKLLIV
CEEEEEEECCCCCEECCCEEEEEECCCCCCCCCCCCCCEEEEECCCEEECCCCCCEEEEE
GGGAIGCEFATLYKTFGSEVVLIEAMETLLPREDRDAGKKLQAAFEAQGITVRTGTSIDR
ECCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCE
LIVVDEKIRIQFDNGDSIDSIDKVLVGIGRAPNIEGLNLEAAGLRTEHGAVKVNELMQTN
EEEEECEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCEEHHHHHHCC
VPHIYAIGDVTGGLTLAHAAQREAQLLVQNLLHGSSHALKELAVPRVAFSYPEVAAVGAS
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHEECCCCCEEECCC
QEGNGIRAYTLPQVPNGRSVVDKVAPAFVKLFLKEQTSEIAGAVIIGEAATEIIHEMALA
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHH
VENGLTLQQVGNTVHVHPTHSKNILYAIQSCG
HHCCCCHHHCCCEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]