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The map label for this gene is tig

Identifier: 78223081

GI number: 78223081

Start: 2088739

End: 2090040

Strand: Reverse

Name: tig

Synonym: Gmet_1874

Alternate gene names: 78223081

Gene position: 2090040-2088739 (Counterclockwise)

Preceding gene: 78223082

Following gene: 78223080

Centisome position: 52.28

GC content: 50.61

Gene sequence:

>1302_bases
ATGACGAGTACTGTCGAGTCTCTCAGCAGCGTGAAAAAAAAGATTTCTTTCGAAATTCCGGCGGAGCGTGTTTCTGCCGA
GATAGAAAAGGTTTTTGGTCAGATTCAGAAGCGTGCCGCCATCAAAGGGTTCAGGAAGGGAAAGGTACCCCGTTCCCTTG
TGGAACAGAATTACCGGTCGATGATGGAGAGTGACGTTCTCAAGAACCTTTTCGACGAAACCTATTTCAAGGCCTTGGCC
GATCATAAAATATTTCCTGTTTCCCATCCTCACATCGAAAGTGATGAAGTGAAGCGTGGTGAAGCGTTGAAATATTCAGC
AACGGTTGAGGTTTTTCCGGAGATTGATGTCAAGGATTACAAAGGTCTCGAGGTGAAGAGGGAAAGATTTGTTTCCGATG
TTGCACCCGTGGAGGCTCGCCTCAACGAGATGCGGGAAGGGATGGCCGAACTCAAGCCGCTGGAGGAAGGTAAATGCGCC
GAGACTGGCAATTTCGTCGTGATTGATTTTGTCGGGTCTGTCGACGGCATACCCTTTGAGGGCGGAGCTGCCGAAAGCTA
TCAACTCGAACTTGGATCTGGACGTTTCATTCCTGGTTTCGAGGACCAGCTTGTTGGTGTCAAGGCCGGGGAGCAGAGAA
CCGTTGAGGTAACGTTCCCGGAGGAGTACGGGAACAAGGATCTTGCCGGAAAGGCGGCTAGCTTCGCCGTTACGGTCAAG
GAGGCAAAGGTTAAGGAACTCCCGGAGCTTGACGACGAGTTCGCGATGCAGTTTGGTGAGTTTGAGACTCTTGAGCAACT
GAAAACCAAGCTTGCCGAGTTGCACAAAGATCAGGAGACCGCCAGGATCAAAGCTGATGTGCAGGACCGTATCGTCAAGG
CGCTAATCGGAAACAACGAGATTGAAGTCCCGTCGACTCTGGTGGAGCGGCAGCTTCAAATGATGCTGTCGAATATGAAA
AACCGCCTTGCTCAACAGCGCCTTTCCCTGGAGATGATGGGAATGGACGATGAGAGTTTCAAGACCCATTATCGTGATTC
CGCCGAGAGTCAGGTCAAAGGGTCGCTGCTCCTGGAAGCCGTGGCTAAAAAGGAAGGAATCACCGTAAATGATCCTGATG
TCGAGGCTAAGCTTCGTGCCATGGCCGAAGAGGCCGGACAGGATTTTGATCGGGTAAAGGACTTCTACGAGCAGAATCAT
AATGCCAAGGAAAATCTTGTGGCTCATCTGAACGAGGAAAAAGTCTTGGGGTATCTTCTGGAGAATGCTGTCGTAACGGA
AGTGGCCAAGGACGAACTCTAA

Upstream 100 bases:

>100_bases
GGATTCCCCCCCGATAGATATTCAATTCAATTCAATCTGCCTTTCATTCAGTAACAACTCTGTCTGATATTCAAATAATC
CAGAAAAGAAAGGATAAGTG

Downstream 100 bases:

>100_bases
TAACATATGGTTTCCGGAGGGAATATGCTCGTACCGATCGTGGTCGAACAAACCGGCAGGGGAGAGCGCTCTTACGACAT
CTATTCGCGCCTTCTGAAGG

Product: trigger factor

Products: peptidylproline (omega=0)

Alternate protein names: TF

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTSTVESLSSVKKKISFEIPAERVSAEIEKVFGQIQKRAAIKGFRKGKVPRSLVEQNYRSMMESDVLKNLFDETYFKALA
DHKIFPVSHPHIESDEVKRGEALKYSATVEVFPEIDVKDYKGLEVKRERFVSDVAPVEARLNEMREGMAELKPLEEGKCA
ETGNFVVIDFVGSVDGIPFEGGAAESYQLELGSGRFIPGFEDQLVGVKAGEQRTVEVTFPEEYGNKDLAGKAASFAVTVK
EAKVKELPELDDEFAMQFGEFETLEQLKTKLAELHKDQETARIKADVQDRIVKALIGNNEIEVPSTLVERQLQMMLSNMK
NRLAQQRLSLEMMGMDDESFKTHYRDSAESQVKGSLLLEAVAKKEGITVNDPDVEAKLRAMAEEAGQDFDRVKDFYEQNH
NAKENLVAHLNEEKVLGYLLENAVVTEVAKDEL

Sequences:

>Translated_433_residues
MTSTVESLSSVKKKISFEIPAERVSAEIEKVFGQIQKRAAIKGFRKGKVPRSLVEQNYRSMMESDVLKNLFDETYFKALA
DHKIFPVSHPHIESDEVKRGEALKYSATVEVFPEIDVKDYKGLEVKRERFVSDVAPVEARLNEMREGMAELKPLEEGKCA
ETGNFVVIDFVGSVDGIPFEGGAAESYQLELGSGRFIPGFEDQLVGVKAGEQRTVEVTFPEEYGNKDLAGKAASFAVTVK
EAKVKELPELDDEFAMQFGEFETLEQLKTKLAELHKDQETARIKADVQDRIVKALIGNNEIEVPSTLVERQLQMMLSNMK
NRLAQQRLSLEMMGMDDESFKTHYRDSAESQVKGSLLLEAVAKKEGITVNDPDVEAKLRAMAEEAGQDFDRVKDFYEQNH
NAKENLVAHLNEEKVLGYLLENAVVTEVAKDEL
>Mature_432_residues
TSTVESLSSVKKKISFEIPAERVSAEIEKVFGQIQKRAAIKGFRKGKVPRSLVEQNYRSMMESDVLKNLFDETYFKALAD
HKIFPVSHPHIESDEVKRGEALKYSATVEVFPEIDVKDYKGLEVKRERFVSDVAPVEARLNEMREGMAELKPLEEGKCAE
TGNFVVIDFVGSVDGIPFEGGAAESYQLELGSGRFIPGFEDQLVGVKAGEQRTVEVTFPEEYGNKDLAGKAASFAVTVKE
AKVKELPELDDEFAMQFGEFETLEQLKTKLAELHKDQETARIKADVQDRIVKALIGNNEIEVPSTLVERQLQMMLSNMKN
RLAQQRLSLEMMGMDDESFKTHYRDSAESQVKGSLLLEAVAKKEGITVNDPDVEAKLRAMAEEAGQDFDRVKDFYEQNHN
AKENLVAHLNEEKVLGYLLENAVVTEVAKDEL

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=432, Percent_Identity=32.8703703703704, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_GEOMG (Q39UH1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384828.1
- ProteinModelPortal:   Q39UH1
- STRING:   Q39UH1
- GeneID:   3740272
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1874
- NMPDR:   fig|269799.3.peg.2137
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q39UH1
- ProtClustDB:   CLSK828562
- BioCyc:   GMET269799:GMET_1874-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: 5.2.1.8

Molecular weight: Translated: 48640; Mature: 48509

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50059 FKBP_PPIASE; PS00014 ER_TARGET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTVESLSSVKKKISFEIPAERVSAEIEKVFGQIQKRAAIKGFRKGKVPRSLVEQNYRS
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MMESDVLKNLFDETYFKALADHKIFPVSHPHIESDEVKRGEALKYSATVEVFPEIDVKDY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCC
KGLEVKRERFVSDVAPVEARLNEMREGMAELKPLEEGKCAETGNFVVIDFVGSVDGIPFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
GGAAESYQLELGSGRFIPGFEDQLVGVKAGEQRTVEVTFPEEYGNKDLAGKAASFAVTVK
CCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCHHHCCCCCCCCHHHEEEEEH
EAKVKELPELDDEFAMQFGEFETLEQLKTKLAELHKDQETARIKADVQDRIVKALIGNNE
HHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
IEVPSTLVERQLQMMLSNMKNRLAQQRLSLEMMGMDDESFKTHYRDSAESQVKGSLLLEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHH
VAKKEGITVNDPDVEAKLRAMAEEAGQDFDRVKDFYEQNHNAKENLVAHLNEEKVLGYLL
HHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
ENAVVTEVAKDEL
HHHHHHHHHHCCC
>Mature Secondary Structure 
TSTVESLSSVKKKISFEIPAERVSAEIEKVFGQIQKRAAIKGFRKGKVPRSLVEQNYRS
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
MMESDVLKNLFDETYFKALADHKIFPVSHPHIESDEVKRGEALKYSATVEVFPEIDVKDY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCC
KGLEVKRERFVSDVAPVEARLNEMREGMAELKPLEEGKCAETGNFVVIDFVGSVDGIPFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
GGAAESYQLELGSGRFIPGFEDQLVGVKAGEQRTVEVTFPEEYGNKDLAGKAASFAVTVK
CCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCHHHCCCCCCCCHHHEEEEEH
EAKVKELPELDDEFAMQFGEFETLEQLKTKLAELHKDQETARIKADVQDRIVKALIGNNE
HHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
IEVPSTLVERQLQMMLSNMKNRLAQQRLSLEMMGMDDESFKTHYRDSAESQVKGSLLLEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHH
VAKKEGITVNDPDVEAKLRAMAEEAGQDFDRVKDFYEQNHNAKENLVAHLNEEKVLGYLL
HHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
ENAVVTEVAKDEL
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA