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The map label for this gene is carA [H]

Identifier: 78222980

GI number: 78222980

Start: 1986259

End: 1987383

Strand: Direct

Name: carA [H]

Synonym: Gmet_1771

Alternate gene names: 78222980

Gene position: 1986259-1987383 (Clockwise)

Preceding gene: 304570571

Following gene: 78222981

Centisome position: 49.69

GC content: 59.02

Gene sequence:

>1125_bases
ATGAAAGCAGTCCTAGCGCTCGCTGATGGGCGCATTTTTGAAGGGAAATCGTTCGGAGCCACCGGCGAGGCGACTGGCGA
GGTGGTCTTCAATACCGCCATGACCGGCTACCAGGAGGTTTTGACCGATCCCTCCTATCGGGGGCAGATGGTCACCATGA
CCTACACCCAGATGGGCAACACCGGCATTAATCCGGAGGATATCGAGAGCAAGCAGCTCTACCTCTCGGGTTTCATCGTC
AAGGAGTACCACGACTGCTACTCCAACTGGCGGGCCACCATGAGCCTAGACGCCTATCTGAAGGAGAACGGCGTGGTGGG
GATCCAGGGGCTCGACACCCGGGCGCTCACCAGACATCTGCGGGACAAGGGGGCCCAGAACGGGATCATCTCTACCGTCG
ATTTCGACCACGAGAGCCTCGTAAAGAAAGCACGGGCCGTGCCGAGCATGGCCGGTCTCGACCTCGCCTCCGGCGTCACC
TGCGATAAGCCCTACCATTGGACCGAAAAGCTCTGGGACCTGGAGGCAGGGTATAACGCTGCGAAACAGGAAGACTTGAA
GTACAAGGTGGTGGCCTACGATTTCGGCATCAAGTACAACATCCTCCGCTGCCTCGTCTCGGCCGGCTGCGACGTGACTG
TTGTCCCCGCCACCTTCCCGGCGGAGGAGGCCCTGGCCATGAACCCTGACGGTATCTTCCTCAGCAACGGTCCCGGCGAT
CCGGAGCCCATGACCACCGTCATCGAGAACATCAGGAAATTCGTGGGCAAGAAGCCCATCTTCGGCATCTGTCTCGGCCA
CCAGCTCCTGGGGCTTGCCCTCGGCGGCAAGACTATGAAACTCAAGTTCGGCAACCACGGCTCCAACCTGCCGGTCATGG
ATATGGATACCCGGAAGGTTGAAATAACCGCCCAGAACCATGGCTTCTCAGTGGATATTGTGTCACTGGGACATGCCTGC
GAGTTGGCCCACGAGAACCTGAACGATCAGACCGTGGAGGGGATGAAGCACAAGGATCTCCCCATCTTCTCGGTCCAGCA
CCACCCGGAGGCATCGCCCGGTCCCCATGACTCCCACTATCTGTTCGGGCGGTTCGTGGAACTGATGGAGAAACATAAAT
CGTAG

Upstream 100 bases:

>100_bases
GGTGGGAGGTGAAGGGGGCCGCGGCGTTCACCATTGTCGGCGGAAAGGTTGTGTATAAACGTTGATGGTTGATTCAATAT
TGCCACACAGGAGTAAGTTC

Downstream 100 bases:

>100_bases
GGGCGCAATTCGTTGCGCCCTGAGCGTGATCAATCACGCTCCTACACAACCATGCGGTACATAGATCTTTATCAAGCAGG
CGAATTGCTGAAGCGGGTCA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKAVLALADGRIFEGKSFGATGEATGEVVFNTAMTGYQEVLTDPSYRGQMVTMTYTQMGNTGINPEDIESKQLYLSGFIV
KEYHDCYSNWRATMSLDAYLKENGVVGIQGLDTRALTRHLRDKGAQNGIISTVDFDHESLVKKARAVPSMAGLDLASGVT
CDKPYHWTEKLWDLEAGYNAAKQEDLKYKVVAYDFGIKYNILRCLVSAGCDVTVVPATFPAEEALAMNPDGIFLSNGPGD
PEPMTTVIENIRKFVGKKPIFGICLGHQLLGLALGGKTMKLKFGNHGSNLPVMDMDTRKVEITAQNHGFSVDIVSLGHAC
ELAHENLNDQTVEGMKHKDLPIFSVQHHPEASPGPHDSHYLFGRFVELMEKHKS

Sequences:

>Translated_374_residues
MKAVLALADGRIFEGKSFGATGEATGEVVFNTAMTGYQEVLTDPSYRGQMVTMTYTQMGNTGINPEDIESKQLYLSGFIV
KEYHDCYSNWRATMSLDAYLKENGVVGIQGLDTRALTRHLRDKGAQNGIISTVDFDHESLVKKARAVPSMAGLDLASGVT
CDKPYHWTEKLWDLEAGYNAAKQEDLKYKVVAYDFGIKYNILRCLVSAGCDVTVVPATFPAEEALAMNPDGIFLSNGPGD
PEPMTTVIENIRKFVGKKPIFGICLGHQLLGLALGGKTMKLKFGNHGSNLPVMDMDTRKVEITAQNHGFSVDIVSLGHAC
ELAHENLNDQTVEGMKHKDLPIFSVQHHPEASPGPHDSHYLFGRFVELMEKHKS
>Mature_374_residues
MKAVLALADGRIFEGKSFGATGEATGEVVFNTAMTGYQEVLTDPSYRGQMVTMTYTQMGNTGINPEDIESKQLYLSGFIV
KEYHDCYSNWRATMSLDAYLKENGVVGIQGLDTRALTRHLRDKGAQNGIISTVDFDHESLVKKARAVPSMAGLDLASGVT
CDKPYHWTEKLWDLEAGYNAAKQEDLKYKVVAYDFGIKYNILRCLVSAGCDVTVVPATFPAEEALAMNPDGIFLSNGPGD
PEPMTTVIENIRKFVGKKPIFGICLGHQLLGLALGGKTMKLKFGNHGSNLPVMDMDTRKVEITAQNHGFSVDIVSLGHAC
ELAHENLNDQTVEGMKHKDLPIFSVQHHPEASPGPHDSHYLFGRFVELMEKHKS

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=37.434554973822, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI169790915, Length=388, Percent_Identity=37.3711340206186, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI21361331, Length=388, Percent_Identity=37.3711340206186, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=53.7433155080214, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1789760, Length=182, Percent_Identity=27.4725274725275, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=395, Percent_Identity=36.9620253164557, Blast_Score=241, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=388, Percent_Identity=40.4639175257732, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=35.8585858585859, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=37.4677002583979, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=37.4677002583979, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41105; Mature: 41105

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVLALADGRIFEGKSFGATGEATGEVVFNTAMTGYQEVLTDPSYRGQMVTMTYTQMGN
CCEEEEECCCEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEEEEECCC
TGINPEDIESKQLYLSGFIVKEYHDCYSNWRATMSLDAYLKENGVVGIQGLDTRALTRHL
CCCCCCCCCCCEEEEEEEHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCHHHHHHHH
RDKGAQNGIISTVDFDHESLVKKARAVPSMAGLDLASGVTCDKPYHWTEKLWDLEAGYNA
HHCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHCCCCC
AKQEDLKYKVVAYDFGIKYNILRCLVSAGCDVTVVPATFPAEEALAMNPDGIFLSNGPGD
CCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCHHEEECCCCEEEECCCCC
PEPMTTVIENIRKFVGKKPIFGICLGHQLLGLALGGKTMKLKFGNHGSNLPVMDMDTRKV
CCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHEECCEEEEEEECCCCCCCCEEECCCCEE
EITAQNHGFSVDIVSLGHACELAHENLNDQTVEGMKHKDLPIFSVQHHPEASPGPHDSHY
EEEECCCCEEEEEEECCCHHHHHHCCCCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCHH
LFGRFVELMEKHKS
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKAVLALADGRIFEGKSFGATGEATGEVVFNTAMTGYQEVLTDPSYRGQMVTMTYTQMGN
CCEEEEECCCEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEEEEECCC
TGINPEDIESKQLYLSGFIVKEYHDCYSNWRATMSLDAYLKENGVVGIQGLDTRALTRHL
CCCCCCCCCCCEEEEEEEHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCHHHHHHHH
RDKGAQNGIISTVDFDHESLVKKARAVPSMAGLDLASGVTCDKPYHWTEKLWDLEAGYNA
HHCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHCCCCC
AKQEDLKYKVVAYDFGIKYNILRCLVSAGCDVTVVPATFPAEEALAMNPDGIFLSNGPGD
CCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCHHEEECCCCEEEECCCCC
PEPMTTVIENIRKFVGKKPIFGICLGHQLLGLALGGKTMKLKFGNHGSNLPVMDMDTRKV
CCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHEECCEEEEEEECCCCCCCCEEECCCCEE
EITAQNHGFSVDIVSLGHACELAHENLNDQTVEGMKHKDLPIFSVQHHPEASPGPHDSHY
EEEECCCCEEEEEEECCCHHHHHHCCCCCHHHCCCCCCCCCEEEEECCCCCCCCCCCCHH
LFGRFVELMEKHKS
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA