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The map label for this gene is gltX

Identifier: 78222945

GI number: 78222945

Start: 1951388

End: 1952785

Strand: Direct

Name: gltX

Synonym: Gmet_1736

Alternate gene names: 78222945

Gene position: 1951388-1952785 (Clockwise)

Preceding gene: 78222944

Following gene: 78222948

Centisome position: 48.82

GC content: 60.8

Gene sequence:

>1398_bases
ATGTCCGACGTACGCCTCCGCTTCGCGCCGAGCCCCACCGGTTACCTTCACGTTGGGGGGGCGCGTACCGCGCTTTTCAA
CTGGCTCCTGTCCCGCAAGGAAAAAGGGACTTTCATCCTCCGCATCGAGGACACCGACGTGGCCCGTTCCACCCAGGAAT
CGGTGGACGCCATCCTTCAGGGGATGGCCTGGCTTGGCCTCGGCTGGGACGAAGGTCCCTTCTACCAGTCGGAGCGTTTT
CCCGTCTACAAGGAGTTCGTGGAGAAGCTCGTGGCCGAGGGTAAGGCATACCGTTGCTACTGCACCTCCGAGGCGCTGGA
GGCCAAGCGGGAGCTGGCCATGAAGGAGGGGCGCAAGCCCAAGTACGACGGCACCTGTCGCAACGGAGTGGAGGAGCCCG
GGGATAAGCCGTACGTAATCCGTTTCAAGGCGCCCCACGAAGGGGTAACGGTCTTCGATGACCTCATCAAGGGTCGGATT
GCCTTCAATAACGACGAACTGGACGACCTCATCATCCAGCGCAGTGACGGCACCCCCACCTACAACTTCGTTGTGGTTAT
CGACGACGCCACCATGGGGATAACCACCGTTATCCGGGGCGATGACCATATCAACAACACCCCTCGCCAGATTCTTCTCT
ACGAGGCCCTTGGCTATCCCGTGCCCCGCTTTGCCCACGTGCCGATGATCCTCGGCGCCGACAAGACCCGTCTCTCCAAG
CGCCACGGCGCCACGAGCGTCATGGCCTACCGTGACATGGGGTTTCTCCCCGAAGCCATGGTCAACTACCTGGTGCGGCT
TGGCTGGAGTCACGGCGATCAGGAGATCTTCAGTCGAGAGGAACTCATCGAGAAGTTTTCCATCGAGGCGGTGGGCAAAT
CTGCCGGAGTATTCAATCCTGACAAGCTCCTCTGGCTCAACCATCACTACATCAAGGAGAGCGCTTCCGAGCGGCTGGCG
GAGCTTTTAATCCCCTTCATGAAGGAGCGTGGCATTGTTCCCGAGTCGGGACCGAGCCTTTCCCTCGTGGTGAAGACCCT
CCAGGAGCGTTCCCGGACCCTCGTGGAGATGGCTGACGGCGCGATTTTTTACTACCGAGGCGATTTTGCCTATGACGAAC
AGGCTGCGGCCAAGCACCTGGTTCCCGATGCCGCACCACTGCTGGAAGCGTTGGCGGACAAACTGGAGGCGCTCGCCGAC
TTCAGTCAGGCGAGCATAGAGGCTCTGTTCAAGGAGTTCATCGCTGAGAAGGGGATCAAGCTGGGGCAGGTGGGGCCGGC
GGTTCGTGTCTCCCTCTGTGGCGGCACCGCATCCCCCGGCATCTACGAGGTAATCGAGGCCCTGGGGAAAGAAGAAACCC
TGCGGAGGCTGCGCCGCGCCGTGGCTTTTATCGGGTAA

Upstream 100 bases:

>100_bases
ACCATGGATCTTGCCGCCTGGGCCGATGAGGAATACCGCATCGCCAGTGAGGATACCCCTGGCGATGATGAATCTGACGA
AAACCCGTAAAGGAGTTCCC

Downstream 100 bases:

>100_bases
CGCAGCAAGCCTCACAGGTCATGGGCGAAAATCAAAGGGGCGGCACGATATGATTTCGTGTCGCCCCTTTTTCCGGTTAG
GGTGGTTTACTTGTAACGCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSDVRLRFAPSPTGYLHVGGARTALFNWLLSRKEKGTFILRIEDTDVARSTQESVDAILQGMAWLGLGWDEGPFYQSERF
PVYKEFVEKLVAEGKAYRCYCTSEALEAKRELAMKEGRKPKYDGTCRNGVEEPGDKPYVIRFKAPHEGVTVFDDLIKGRI
AFNNDELDDLIIQRSDGTPTYNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSK
RHGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDQEIFSREELIEKFSIEAVGKSAGVFNPDKLLWLNHHYIKESASERLA
ELLIPFMKERGIVPESGPSLSLVVKTLQERSRTLVEMADGAIFYYRGDFAYDEQAAAKHLVPDAAPLLEALADKLEALAD
FSQASIEALFKEFIAEKGIKLGQVGPAVRVSLCGGTASPGIYEVIEALGKEETLRRLRRAVAFIG

Sequences:

>Translated_465_residues
MSDVRLRFAPSPTGYLHVGGARTALFNWLLSRKEKGTFILRIEDTDVARSTQESVDAILQGMAWLGLGWDEGPFYQSERF
PVYKEFVEKLVAEGKAYRCYCTSEALEAKRELAMKEGRKPKYDGTCRNGVEEPGDKPYVIRFKAPHEGVTVFDDLIKGRI
AFNNDELDDLIIQRSDGTPTYNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSK
RHGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDQEIFSREELIEKFSIEAVGKSAGVFNPDKLLWLNHHYIKESASERLA
ELLIPFMKERGIVPESGPSLSLVVKTLQERSRTLVEMADGAIFYYRGDFAYDEQAAAKHLVPDAAPLLEALADKLEALAD
FSQASIEALFKEFIAEKGIKLGQVGPAVRVSLCGGTASPGIYEVIEALGKEETLRRLRRAVAFIG
>Mature_464_residues
SDVRLRFAPSPTGYLHVGGARTALFNWLLSRKEKGTFILRIEDTDVARSTQESVDAILQGMAWLGLGWDEGPFYQSERFP
VYKEFVEKLVAEGKAYRCYCTSEALEAKRELAMKEGRKPKYDGTCRNGVEEPGDKPYVIRFKAPHEGVTVFDDLIKGRIA
FNNDELDDLIIQRSDGTPTYNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSKR
HGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDQEIFSREELIEKFSIEAVGKSAGVFNPDKLLWLNHHYIKESASERLAE
LLIPFMKERGIVPESGPSLSLVVKTLQERSRTLVEMADGAIFYYRGDFAYDEQAAAKHLVPDAAPLLEALADKLEALADF
SQASIEALFKEFIAEKGIKLGQVGPAVRVSLCGGTASPGIYEVIEALGKEETLRRLRRAVAFIG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=492, Percent_Identity=30.6910569105691, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI62241042, Length=255, Percent_Identity=27.4509803921569, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=53.6637931034483, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI226510925, Length=251, Percent_Identity=35.8565737051793, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1786895, Length=336, Percent_Identity=23.5119047619048, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=32.2851153039832, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17511007, Length=253, Percent_Identity=25.6916996047431, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=34.4897959183673, Blast_Score=272, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321192, Length=266, Percent_Identity=25.9398496240602, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324742, Length=159, Percent_Identity=32.0754716981132, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24665321, Length=496, Percent_Identity=34.6774193548387, Blast_Score=279, Evalue=3e-75,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_GEOMG (Q39UV7)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384692.1
- ProteinModelPortal:   Q39UV7
- SMR:   Q39UV7
- STRING:   Q39UV7
- GeneID:   3741412
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1736
- NMPDR:   fig|269799.3.peg.2053
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q39UV7
- ProtClustDB:   PRK01406
- BioCyc:   GMET269799:GMET_1736-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51922; Mature: 51791

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVRLRFAPSPTGYLHVGGARTALFNWLLSRKEKGTFILRIEDTDVARSTQESVDAILQ
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHH
GMAWLGLGWDEGPFYQSERFPVYKEFVEKLVAEGKAYRCYCTSEALEAKRELAMKEGRKP
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCC
KYDGTCRNGVEEPGDKPYVIRFKAPHEGVTVFDDLIKGRIAFNNDELDDLIIQRSDGTPT
CCCCCHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCEEEECCCCHHHHEEECCCCCCC
YNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSK
EEEEEEECCCCCCEEEEEECCCCCCCCHHHEEEEHHHCCCCCCHHCCCEEECCCHHHHHH
RHGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDQEIFSREELIEKFSIEAVGKSAGVFNP
HCCCCCEEHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DKLLWLNHHYIKESASERLAELLIPFMKERGIVPESGPSLSLVVKTLQERSRTLVEMADG
CCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
AIFYYRGDFAYDEQAAAKHLVPDAAPLLEALADKLEALADFSQASIEALFKEFIAEKGIK
EEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LGQVGPAVRVSLCGGTASPGIYEVIEALGKEETLRRLRRAVAFIG
CCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVRLRFAPSPTGYLHVGGARTALFNWLLSRKEKGTFILRIEDTDVARSTQESVDAILQ
CCCEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHH
GMAWLGLGWDEGPFYQSERFPVYKEFVEKLVAEGKAYRCYCTSEALEAKRELAMKEGRKP
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCC
KYDGTCRNGVEEPGDKPYVIRFKAPHEGVTVFDDLIKGRIAFNNDELDDLIIQRSDGTPT
CCCCCHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCEEEECCCCHHHHEEECCCCCCC
YNFVVVIDDATMGITTVIRGDDHINNTPRQILLYEALGYPVPRFAHVPMILGADKTRLSK
EEEEEEECCCCCCEEEEEECCCCCCCCHHHEEEEHHHCCCCCCHHCCCEEECCCHHHHHH
RHGATSVMAYRDMGFLPEAMVNYLVRLGWSHGDQEIFSREELIEKFSIEAVGKSAGVFNP
HCCCCCEEHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DKLLWLNHHYIKESASERLAELLIPFMKERGIVPESGPSLSLVVKTLQERSRTLVEMADG
CCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
AIFYYRGDFAYDEQAAAKHLVPDAAPLLEALADKLEALADFSQASIEALFKEFIAEKGIK
EEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LGQVGPAVRVSLCGGTASPGIYEVIEALGKEETLRRLRRAVAFIG
CCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA