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The map label for this gene is glpK

Identifier: 78222885

GI number: 78222885

Start: 1882926

End: 1884416

Strand: Direct

Name: glpK

Synonym: Gmet_1676

Alternate gene names: 78222885

Gene position: 1882926-1884416 (Clockwise)

Preceding gene: 78222880

Following gene: 78222886

Centisome position: 47.1

GC content: 69.42

Gene sequence:

>1491_bases
ATGAGTTATATCCTTGCCCTGGATCAGGGCACTACCAGTTCCCGCGCCATTGTCTTCGACACCACCGCAACGATCCGCGC
CATTGCCCAGCGGGAGTTCCGCCAGATATTTCCCCGGCCCGGCTGGGTGGAACACGACGGCCGGGAGATCTGGGCCACCC
AGGTGGGCGTGGCGGCCGAAGCCCTGACCCGGGCCGGCATCTCTCCCCGGGAGGTACGGGCCATCGGCATCACCAACCAG
CGCGAGACAACGCTCCTCTGGGACCGACGAAGCGGCGAGCCGCTCCACAACGCCATCGTCTGGCAGGACCGCCGCACCGC
GGAACTCTGCGACCGGCTCAAGGCCGACGGCCTGGAACCCCTGTTCCGGCAGAAGACCGGCCTGGTACTGGATGCCTACT
TTTCCGGCACCAAGCTGGCCTGGCTGCTGGAGGCCATACCTGGCGCCCGCAGCCGCGCCGAAGCGGGGGAGCTGGCCTTC
GGCACGGTGGATTCCTGGCTGGCCTGGAACCTGTCGGGAGGAAAGCTCCATGTCACCGACGCCAGCAACGCCTCCCGGAC
GCTCCTCTTCAACATCACTACCCTGGATTGGGACGACGAGCTGTTGGCGATCCTCGGGATACCCCGGGCAGTGCTGCCGC
GGGTGGTGGATTCCGGCGCCGTTTACGGCGAGACCGCCGGGGATATTTTTGCTGCGGCGATTCCCCTTGCCGGCATGGCC
GGGGACCAGCAGGCGGCCCTCTTCGGGCAGGCCTGCGGCACGGCAGGGATGGCCAAGAACACCTACGGCACCGGCTGCTT
CCTGCTGATGCACTGTGGAACACGGCCGATAGAGTCCCGGCACAACCTGCTCACCACCGTTGCCTGGCAACTGGGCGGAC
GGGCTGAATACGCCCTTGAGGGGAGCGTGTTTGTGGCCGGCGCTGCGGTGCAGTGGCTCCGGGACGGCCTGGGCATCATC
CGCTCCTCCGGCGAGGTGGAGGCCCTTGCGGCCAGCGTTCCGGACAGCGGCGGTGTCTGCCTGGTTCCCGCCTTCACCGG
ACTCGGCGCCCCCCACTGGGATCCCTACGCCCGGGGCACCATCGTCGGCATAACCCGCGGCACCACCGCGGCACACATCG
CCCGGGCCACCCTGGAGAGCATCGCCTTCCAGAGCGCCGACCTCCTCACCGCCATGGAGGCCGACGCCGGCATCAGCCTG
GCCGAATTGCGGGTCGACGGAGGCGCAACGGCCAACGGGCTCCTGATGCAGTTCCAGGCCGACCTCCTCGGGGTGCCGGT
GGTGCGGCCCCGGATCAGCGAGACCACGGCCCTGGGGGCTGCCTACCTGGCGGGACTGGCGGTGGGGCTCTGGCGTGACC
GTCAGGAGATTGCCGGCCACTGGCAGGTGGACCGGATCTTCGAGCCGGCCATGGGCCGCGAGCAGGCGGCAGAGCTACGG
GGAAGGTGGGGGCGGGCCGTGGAGCGGGCCCGGGGGTGGGCGGCGCCATGA

Upstream 100 bases:

>100_bases
CGTGGAAAAGAAAGGGTGTAAAAATCGGAGTCCCTCTTGCGCAACCCGTCTCTTCACGGGACAATTGCGATACAGCAGCC
AACTATTCCGCGAGGTGGCC

Downstream 100 bases:

>100_bases
CCCGTACGAGGCTCTTGCAACAGCTTGAAGAGGAGAAGTGCTGGGATCTCCTCGTTATCGGCGGGGGCGCCACCGGGCTC
GGCGTGGCCGTTGAGGGGGC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSYILALDQGTTSSRAIVFDTTATIRAIAQREFRQIFPRPGWVEHDGREIWATQVGVAAEALTRAGISPREVRAIGITNQ
RETTLLWDRRSGEPLHNAIVWQDRRTAELCDRLKADGLEPLFRQKTGLVLDAYFSGTKLAWLLEAIPGARSRAEAGELAF
GTVDSWLAWNLSGGKLHVTDASNASRTLLFNITTLDWDDELLAILGIPRAVLPRVVDSGAVYGETAGDIFAAAIPLAGMA
GDQQAALFGQACGTAGMAKNTYGTGCFLLMHCGTRPIESRHNLLTTVAWQLGGRAEYALEGSVFVAGAAVQWLRDGLGII
RSSGEVEALAASVPDSGGVCLVPAFTGLGAPHWDPYARGTIVGITRGTTAAHIARATLESIAFQSADLLTAMEADAGISL
AELRVDGGATANGLLMQFQADLLGVPVVRPRISETTALGAAYLAGLAVGLWRDRQEIAGHWQVDRIFEPAMGREQAAELR
GRWGRAVERARGWAAP

Sequences:

>Translated_496_residues
MSYILALDQGTTSSRAIVFDTTATIRAIAQREFRQIFPRPGWVEHDGREIWATQVGVAAEALTRAGISPREVRAIGITNQ
RETTLLWDRRSGEPLHNAIVWQDRRTAELCDRLKADGLEPLFRQKTGLVLDAYFSGTKLAWLLEAIPGARSRAEAGELAF
GTVDSWLAWNLSGGKLHVTDASNASRTLLFNITTLDWDDELLAILGIPRAVLPRVVDSGAVYGETAGDIFAAAIPLAGMA
GDQQAALFGQACGTAGMAKNTYGTGCFLLMHCGTRPIESRHNLLTTVAWQLGGRAEYALEGSVFVAGAAVQWLRDGLGII
RSSGEVEALAASVPDSGGVCLVPAFTGLGAPHWDPYARGTIVGITRGTTAAHIARATLESIAFQSADLLTAMEADAGISL
AELRVDGGATANGLLMQFQADLLGVPVVRPRISETTALGAAYLAGLAVGLWRDRQEIAGHWQVDRIFEPAMGREQAAELR
GRWGRAVERARGWAAP
>Mature_495_residues
SYILALDQGTTSSRAIVFDTTATIRAIAQREFRQIFPRPGWVEHDGREIWATQVGVAAEALTRAGISPREVRAIGITNQR
ETTLLWDRRSGEPLHNAIVWQDRRTAELCDRLKADGLEPLFRQKTGLVLDAYFSGTKLAWLLEAIPGARSRAEAGELAFG
TVDSWLAWNLSGGKLHVTDASNASRTLLFNITTLDWDDELLAILGIPRAVLPRVVDSGAVYGETAGDIFAAAIPLAGMAG
DQQAALFGQACGTAGMAKNTYGTGCFLLMHCGTRPIESRHNLLTTVAWQLGGRAEYALEGSVFVAGAAVQWLRDGLGIIR
SSGEVEALAASVPDSGGVCLVPAFTGLGAPHWDPYARGTIVGITRGTTAAHIARATLESIAFQSADLLTAMEADAGISLA
ELRVDGGATANGLLMQFQADLLGVPVVRPRISETTALGAAYLAGLAVGLWRDRQEIAGHWQVDRIFEPAMGREQAAELRG
RWGRAVERARGWAAP

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=503, Percent_Identity=46.3220675944334, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI4504007, Length=503, Percent_Identity=46.3220675944334, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=45.956607495069, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=44.6215139442231, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=32.741617357002, Blast_Score=317, Evalue=2e-86,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=57.606490872211, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1790005, Length=483, Percent_Identity=26.7080745341615, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787789, Length=479, Percent_Identity=27.348643006263, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789987, Length=484, Percent_Identity=24.1735537190083, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789168, Length=498, Percent_Identity=25.3012048192771, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789138, Length=483, Percent_Identity=22.360248447205, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=506, Percent_Identity=42.8853754940711, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=27.063339731286, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI71986147, Length=301, Percent_Identity=32.5581395348837, Blast_Score=138, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6321755, Length=547, Percent_Identity=38.7568555758684, Blast_Score=333, Evalue=5e-92,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=43.9453125, Blast_Score=407, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24655555, Length=501, Percent_Identity=41.7165668662675, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655560, Length=501, Percent_Identity=41.7165668662675, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655570, Length=501, Percent_Identity=41.7165668662675, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655574, Length=501, Percent_Identity=41.7165668662675, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655565, Length=501, Percent_Identity=41.7165668662675, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24654573, Length=453, Percent_Identity=45.0331125827815, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=33.0170777988615, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=33.2657200811359, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24656367, Length=522, Percent_Identity=32.9501915708812, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI21357867, Length=527, Percent_Identity=33.2068311195446, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=33.810888252149, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=30.6896551724138, Blast_Score=128, Evalue=8e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_GEOMG (Q39V17)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384632.1
- ProteinModelPortal:   Q39V17
- SMR:   Q39V17
- STRING:   Q39V17
- GeneID:   3740526
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1676
- NMPDR:   fig|269799.3.peg.1993
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- PhylomeDB:   Q39V17
- ProtClustDB:   CLSK743164
- BioCyc:   GMET269799:GMET_1676-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 53062; Mature: 52931

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 133-133 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYILALDQGTTSSRAIVFDTTATIRAIAQREFRQIFPRPGWVEHDGREIWATQVGVAAE
CCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHH
ALTRAGISPREVRAIGITNQRETTLLWDRRSGEPLHNAIVWQDRRTAELCDRLKADGLEP
HHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCHHHCEEECCCCHHHHHHHHHHCCCCH
LFRQKTGLVLDAYFSGTKLAWLLEAIPGARSRAEAGELAFGTVDSWLAWNLSGGKLHVTD
HHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEE
ASNASRTLLFNITTLDWDDELLAILGIPRAVLPRVVDSGAVYGETAGDIFAAAIPLAGMA
CCCCCEEEEEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCC
GDQQAALFGQACGTAGMAKNTYGTGCFLLMHCGTRPIESRHNLLTTVAWQLGGRAEYALE
CCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEC
GSVFVAGAAVQWLRDGLGIIRSSGEVEALAASVPDSGGVCLVPAFTGLGAPHWDPYARGT
CCEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCE
IVGITRGTTAAHIARATLESIAFQSADLLTAMEADAGISLAELRVDGGATANGLLMQFQA
EEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEH
DLLGVPVVRPRISETTALGAAYLAGLAVGLWRDRQEIAGHWQVDRIFEPAMGREQAAELR
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHCCCCCHHHHHHHH
GRWGRAVERARGWAAP
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SYILALDQGTTSSRAIVFDTTATIRAIAQREFRQIFPRPGWVEHDGREIWATQVGVAAE
CEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHH
ALTRAGISPREVRAIGITNQRETTLLWDRRSGEPLHNAIVWQDRRTAELCDRLKADGLEP
HHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCHHHCEEECCCCHHHHHHHHHHCCCCH
LFRQKTGLVLDAYFSGTKLAWLLEAIPGARSRAEAGELAFGTVDSWLAWNLSGGKLHVTD
HHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEE
ASNASRTLLFNITTLDWDDELLAILGIPRAVLPRVVDSGAVYGETAGDIFAAAIPLAGMA
CCCCCEEEEEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCC
GDQQAALFGQACGTAGMAKNTYGTGCFLLMHCGTRPIESRHNLLTTVAWQLGGRAEYALE
CCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEC
GSVFVAGAAVQWLRDGLGIIRSSGEVEALAASVPDSGGVCLVPAFTGLGAPHWDPYARGT
CCEEEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCE
IVGITRGTTAAHIARATLESIAFQSADLLTAMEADAGISLAELRVDGGATANGLLMQFQA
EEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEH
DLLGVPVVRPRISETTALGAAYLAGLAVGLWRDRQEIAGHWQVDRIFEPAMGREQAAELR
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHCCCCCHHHHHHHH
GRWGRAVERARGWAAP
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA