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The map label for this gene is ribE [H]

Identifier: 78222834

GI number: 78222834

Start: 1822589

End: 1823239

Strand: Direct

Name: ribE [H]

Synonym: Gmet_1625

Alternate gene names: 78222834

Gene position: 1822589-1823239 (Clockwise)

Preceding gene: 78222833

Following gene: 78222835

Centisome position: 45.59

GC content: 58.22

Gene sequence:

>651_bases
ATGTTTACCGGGCTCATAGAAGACGTAGGATCGGTAAGAAGGCTTGAACGGGCAGGGGGGGCGGCACGGCTCACCACCGC
CACAAAAATTCCGCTGGATTCCATTGCCCTCGGTGACTCGGTTGCGGTGAATGGGGCCTGCCTCACGGTTGTGGCAAAGG
GAGGAGGCGAACTCACCTTTGACGCCTCCCCGGAAACCCTGGAGCGGACCACTCTCGGCACCCTCGGCACCGGAGCCCCC
GTGAACCTGGAAAGGGCACTGCGTCTTGGCGACCGCCTGGGTGGCCACATCGTCACCGGCCATGTGGACTTTATCGCCGA
TGTCTCCGAACGGCGCGAGGTTGCCGGCAACATTGTCTTCGGTTTCCGCATCCCAGGGCAATTTGCCCGTTATCTGGTGG
CCAAGGGTTCGGTCACCATCGACGGAATCAGTCTTACGGTTAATACCGTCACTGATAGCGGTTTTTCGGTCAATATCATC
CCCCATACCGCTGCAATGACCACCCTGAACGGCATGAAACCGGGTGGACGGGTTAATATTGAAACGGACATTCTGGGGAA
ATATGTCGAGCGCCTCCTGGCCTTTGGAAGCACGGGTCAGAAGGAGGGTGGTCTCAGTCTTGAACTGCTTGCAAAGAGCG
GCTTTCTGTGA

Upstream 100 bases:

>100_bases
GGCCGTCGGCCGAAAGAATGGATGGATGCTGCCGGCTGAAGGATGTAAGCGTGCGGCGTTTCGGCGATGACGTGATGATC
GAGGCTTATCCGGAGGATTT

Downstream 100 bases:

>100_bases
GATATAACGTTGTTATTACTTCTCATGAACGGAGTCGACAATGACGGTTGCTAGAATAGAAGAAGCCATAGAGGACATCA
AGCAGGGGAAAATGATCATT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MFTGLIEDVGSVRRLERAGGAARLTTATKIPLDSIALGDSVAVNGACLTVVAKGGGELTFDASPETLERTTLGTLGTGAP
VNLERALRLGDRLGGHIVTGHVDFIADVSERREVAGNIVFGFRIPGQFARYLVAKGSVTIDGISLTVNTVTDSGFSVNII
PHTAAMTTLNGMKPGGRVNIETDILGKYVERLLAFGSTGQKEGGLSLELLAKSGFL

Sequences:

>Translated_216_residues
MFTGLIEDVGSVRRLERAGGAARLTTATKIPLDSIALGDSVAVNGACLTVVAKGGGELTFDASPETLERTTLGTLGTGAP
VNLERALRLGDRLGGHIVTGHVDFIADVSERREVAGNIVFGFRIPGQFARYLVAKGSVTIDGISLTVNTVTDSGFSVNII
PHTAAMTTLNGMKPGGRVNIETDILGKYVERLLAFGSTGQKEGGLSLELLAKSGFL
>Mature_216_residues
MFTGLIEDVGSVRRLERAGGAARLTTATKIPLDSIALGDSVAVNGACLTVVAKGGGELTFDASPETLERTTLGTLGTGAP
VNLERALRLGDRLGGHIVTGHVDFIADVSERREVAGNIVFGFRIPGQFARYLVAKGSVTIDGISLTVNTVTDSGFSVNII
PHTAAMTTLNGMKPGGRVNIETDILGKYVERLLAFGSTGQKEGGLSLELLAKSGFL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=212, Percent_Identity=37.7358490566038, Blast_Score=126, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319733, Length=206, Percent_Identity=38.3495145631068, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22470; Mature: 22470

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGLIEDVGSVRRLERAGGAARLTTATKIPLDSIALGDSVAVNGACLTVVAKGGGELTF
CCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHEECCCCEEECCEEEEEEECCCCEEEE
DASPETLERTTLGTLGTGAPVNLERALRLGDRLGGHIVTGHVDFIADVSERREVAGNIVF
CCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHHHHCHHHHHHHHCEEEE
GFRIPGQFARYLVAKGSVTIDGISLTVNTVTDSGFSVNIIPHTAAMTTLNGMKPGGRVNI
EEECCHHHHHHHHHCCCEEEEEEEEEEEEEECCCEEEEEEECHHHHHHHCCCCCCCEEEE
ETDILGKYVERLLAFGSTGQKEGGLSLELLAKSGFL
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MFTGLIEDVGSVRRLERAGGAARLTTATKIPLDSIALGDSVAVNGACLTVVAKGGGELTF
CCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHEECCCCEEECCEEEEEEECCCCEEEE
DASPETLERTTLGTLGTGAPVNLERALRLGDRLGGHIVTGHVDFIADVSERREVAGNIVF
CCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHHHHCHHHHHHHHCEEEE
GFRIPGQFARYLVAKGSVTIDGISLTVNTVTDSGFSVNIIPHTAAMTTLNGMKPGGRVNI
EEECCHHHHHHHHHCCCEEEEEEEEEEEEEECCCEEEEEEECHHHHHHHCCCCCCCEEEE
ETDILGKYVERLLAFGSTGQKEGGLSLELLAKSGFL
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]