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The map label for this gene is glyA

Identifier: 78222815

GI number: 78222815

Start: 1804988

End: 1806235

Strand: Direct

Name: glyA

Synonym: Gmet_1605

Alternate gene names: 78222815

Gene position: 1804988-1806235 (Clockwise)

Preceding gene: 78222814

Following gene: 78222818

Centisome position: 45.15

GC content: 57.13

Gene sequence:

>1248_bases
ATGTCGATCCTCGAAACCTTTGACCCGGCAGTAGCCGAGGCCATCCGTCATGAAACCGAGCGCCAGGAGTACAATCTGGA
GCTCATTGCGTCCGAAAACTTTGTCTCCGAAGCAGTGATGGAGGCCCAGGGGTCGGTTCTGACCAACAAATATGCTGAAG
GGTACCCTGGCAAGCGATACTATGGCGGTTGCCACCATGTGGATGTGGTGGAGAACCTTGCAATTGAGCGGGCGAAAGAA
CTTTTTGGTGCCGATCATGCGAACGTCCAGCCCCACTCCGGCTCCCAAGCCAATATGGCGGTTTATTTCTCGGTGCTGAA
GCCGGGCGACACCATTCTCGGCATGAATCTCTCCCACGGCGGCCACCTTACCCACGGTAGCCCGGTGAACTTCTCGGGCC
GGTTCTTCAACGTGGTTCCCTATGGTGTTTCCCAGGAAACCGAGACGATTGACTTCAACGAAGTCGAGCGTCTGGCCCTT
GAGCATAAGCCGAAGCTGATCGTTGTGGGGGCCAGCGCCTATCCACGTGTTCTGGATTTTGCCGCCTTCAGGGCCATAGC
CGACAAAGTGGGGGCGCTGGTCATGGTGGACATGGCCCATATTGCGGGGCTCGTTGCTGCCGGCCTTCACCCAAGCCCGG
TTCCCTATGCCGAGTTTGTCACCACGACAACCCACAAAACCCTGCGTGGACCCCGCGGCGGGATGATTCTCTGCCGCGAG
GAATTTGCCAAGACCCTCAATTCCAATATTTTCCCCGGTATTCAGGGCGGCCCTCTCATGCATGTCATCGCGGCCAAGGC
AGTTGCCTTCAAAGAGGCTCTTGCGCCTGAATTCAAGTTGTATCAGGAGCAGATTGTCAAGAATGCACGGACCCTTGCTG
ACGAACTCATGAAGCGCGGCTTCCGGCTTGTCTCCGGAGGTACCGACAACCACCTGATGCTTGTTAACCTGACGGGCACC
GAGTTGACCGGCAAGGTGGCCGAGGAGGCCCTCGACAAAGCGGGTATCACGGTCAACAAGAACACCGTCCCCTTTGAGAC
ACGCTCTCCCTTTGTTACCTCCGGCTTCCGGATCGGTACTCCTGCTGCCACAAGTCATGGACTCAAAGAGGCGGAGATGG
TGGAAGTTGCCGCTTTCATCGCCGAGGCCTTGGCAAATGTGGGGAACGAGGCAAAGCTTGCAGAGGTAAAGGGGAAAGTG
AACGCCCTTATGGGCCGTTTTCCGCTCTATGCCAGCCGCCTGAAGTAA

Upstream 100 bases:

>100_bases
ATGAAGTAGTCCGGCCGAACCGGCCGACTTGTGGAAGATACGCACTACGGGACTGTCAATGGTCCCGTTTTCCTTTTTGT
AATCGTGAAAGGAGTCAACT

Downstream 100 bases:

>100_bases
CGTTACCACACCATCTTACACAAAGAGGGGGAATGCCACGCTGCATTCCCCCTCTTTGTGTTTGGACTGTCGTGAGTCTT
ACTGTTGTATGAAGACCGCC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSILETFDPAVAEAIRHETERQEYNLELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCHHVDVVENLAIERAKE
LFGADHANVQPHSGSQANMAVYFSVLKPGDTILGMNLSHGGHLTHGSPVNFSGRFFNVVPYGVSQETETIDFNEVERLAL
EHKPKLIVVGASAYPRVLDFAAFRAIADKVGALVMVDMAHIAGLVAAGLHPSPVPYAEFVTTTTHKTLRGPRGGMILCRE
EFAKTLNSNIFPGIQGGPLMHVIAAKAVAFKEALAPEFKLYQEQIVKNARTLADELMKRGFRLVSGGTDNHLMLVNLTGT
ELTGKVAEEALDKAGITVNKNTVPFETRSPFVTSGFRIGTPAATSHGLKEAEMVEVAAFIAEALANVGNEAKLAEVKGKV
NALMGRFPLYASRLK

Sequences:

>Translated_415_residues
MSILETFDPAVAEAIRHETERQEYNLELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCHHVDVVENLAIERAKE
LFGADHANVQPHSGSQANMAVYFSVLKPGDTILGMNLSHGGHLTHGSPVNFSGRFFNVVPYGVSQETETIDFNEVERLAL
EHKPKLIVVGASAYPRVLDFAAFRAIADKVGALVMVDMAHIAGLVAAGLHPSPVPYAEFVTTTTHKTLRGPRGGMILCRE
EFAKTLNSNIFPGIQGGPLMHVIAAKAVAFKEALAPEFKLYQEQIVKNARTLADELMKRGFRLVSGGTDNHLMLVNLTGT
ELTGKVAEEALDKAGITVNKNTVPFETRSPFVTSGFRIGTPAATSHGLKEAEMVEVAAFIAEALANVGNEAKLAEVKGKV
NALMGRFPLYASRLK
>Mature_414_residues
SILETFDPAVAEAIRHETERQEYNLELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCHHVDVVENLAIERAKEL
FGADHANVQPHSGSQANMAVYFSVLKPGDTILGMNLSHGGHLTHGSPVNFSGRFFNVVPYGVSQETETIDFNEVERLALE
HKPKLIVVGASAYPRVLDFAAFRAIADKVGALVMVDMAHIAGLVAAGLHPSPVPYAEFVTTTTHKTLRGPRGGMILCREE
FAKTLNSNIFPGIQGGPLMHVIAAKAVAFKEALAPEFKLYQEQIVKNARTLADELMKRGFRLVSGGTDNHLMLVNLTGTE
LTGKVAEEALDKAGITVNKNTVPFETRSPFVTSGFRIGTPAATSHGLKEAEMVEVAAFIAEALANVGNEAKLAEVKGKVN
ALMGRFPLYASRLK

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=413, Percent_Identity=47.6997578692494, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI261862352, Length=418, Percent_Identity=47.3684210526316, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI261862350, Length=418, Percent_Identity=47.3684210526316, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI261862348, Length=418, Percent_Identity=47.3684210526316, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI261862346, Length=413, Percent_Identity=46.9733656174334, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI22547186, Length=428, Percent_Identity=45.3271028037383, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI22547189, Length=413, Percent_Identity=44.3099273607748, Blast_Score=306, Evalue=2e-83,
Organism=Escherichia coli, GI1788902, Length=411, Percent_Identity=61.5571776155718, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI25144732, Length=404, Percent_Identity=47.7722772277228, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25144729, Length=404, Percent_Identity=47.7722772277228, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319739, Length=412, Percent_Identity=46.1165048543689, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323087, Length=401, Percent_Identity=44.8877805486284, Blast_Score=347, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24640005, Length=410, Percent_Identity=49.2682926829268, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI221329721, Length=410, Percent_Identity=49.2682926829268, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_GEOMG (Q39V87)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384562.1
- ProteinModelPortal:   Q39V87
- SMR:   Q39V87
- STRING:   Q39V87
- GeneID:   3741621
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1605
- NMPDR:   fig|269799.3.peg.1444
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   MALMEPG
- PhylomeDB:   Q39V87
- ProtClustDB:   PRK00011
- BioCyc:   GMET269799:GMET_1605-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44877; Mature: 44746

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILETFDPAVAEAIRHETERQEYNLELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRY
CCCHHHCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCC
YGGCHHVDVVENLAIERAKELFGADHANVQPHSGSQANMAVYFSVLKPGDTILGMNLSHG
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCC
GHLTHGSPVNFSGRFFNVVPYGVSQETETIDFNEVERLALEHKPKLIVVGASAYPRVLDF
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHH
AAFRAIADKVGALVMVDMAHIAGLVAAGLHPSPVPYAEFVTTTTHKTLRGPRGGMILCRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHH
EFAKTLNSNIFPGIQGGPLMHVIAAKAVAFKEALAPEFKLYQEQIVKNARTLADELMKRG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
FRLVSGGTDNHLMLVNLTGTELTGKVAEEALDKAGITVNKNTVPFETRSPFVTSGFRIGT
CEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEECCEEECC
PAATSHGLKEAEMVEVAAFIAEALANVGNEAKLAEVKGKVNALMGRFPLYASRLK
CCHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure 
SILETFDPAVAEAIRHETERQEYNLELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRY
CCHHHCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCC
YGGCHHVDVVENLAIERAKELFGADHANVQPHSGSQANMAVYFSVLKPGDTILGMNLSHG
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCC
GHLTHGSPVNFSGRFFNVVPYGVSQETETIDFNEVERLALEHKPKLIVVGASAYPRVLDF
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHH
AAFRAIADKVGALVMVDMAHIAGLVAAGLHPSPVPYAEFVTTTTHKTLRGPRGGMILCRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHH
EFAKTLNSNIFPGIQGGPLMHVIAAKAVAFKEALAPEFKLYQEQIVKNARTLADELMKRG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
FRLVSGGTDNHLMLVNLTGTELTGKVAEEALDKAGITVNKNTVPFETRSPFVTSGFRIGT
CEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEECCEEECC
PAATSHGLKEAEMVEVAAFIAEALANVGNEAKLAEVKGKVNALMGRFPLYASRLK
CCHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA