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The map label for this gene is cysK [H]

Identifier: 78222770

GI number: 78222770

Start: 1762700

End: 1763629

Strand: Direct

Name: cysK [H]

Synonym: Gmet_1558

Alternate gene names: 78222770

Gene position: 1762700-1763629 (Clockwise)

Preceding gene: 78222754

Following gene: 78222771

Centisome position: 44.1

GC content: 60.11

Gene sequence:

>930_bases
ATGGCACAAATTTACGAGAATCTTACTGACCTGATCGGCAAAACTCCGTTGCTGAGGCTTCGCAAAGTGGCCGCGGGAAT
CGAGGCAGACATTGTAGCGAAGCTGGAATCGTTCAACCCCGGCGGCAGCGTCAAGGACCGGATCGGCGTGTCCATGATCC
GTGATGCCGAAGGGAGGGGACTCATCAACAAAGATACGGTCATTATCGAGCCGACCAGTGGCAACACCGGCATCGCTCTG
GCCTTCGTCTGCGCGGCAAGAGGATACCGCCTGGTCCTCACCATGCCCGATACCATGAGCGTCGAGCGGCGCAATCTCCT
GAAGGCCTACGGCGCCGAACTGGTTCTCACCCGGGGGGCGGAAGGGATGAAGGGTGCGATCAATAAAGCCGAGGAACTGG
CCGCGGATATCCCCAACTCGTTCATACCCCAGCAGTTCCGGAACCCGGCTAACCCCCACATCCATCGTTTCACCACCGCT
GAGGAGATCTGGACGGACACCGACGGCAAAGTCGACGTTTTCGTGGCCGGCGTCGGAACCGGCGGAACCGTGACCGGTGT
CGGCGAGGTGCTCAAAAAGAGAAACCCGGGGGTGAAAATCATCGCCGTGGAGCCGTTCGATTCACCGGTCCTTTCCGGCG
GCCATCCCGCCCCACACAAAATCCAGGGAATCGGCGCCGGTTTCGTGCCGGAGGTATTGAACCGTGAAGCCATTGACGAA
ATCTTCACGGTGAGAAACGAGGAGGCGCTGGAAACCGCCCGCCGTCTGGCCCGAGAAGAGGGGCTGCTTGTGGGGATCTC
CGCGGGAGCGGCGGCTTTCGCCGCCCTGGAGGTGGCGAGGCGTCCCGAAAACAAGGGGAAGAGAATTGTTGTTGTCCTTC
CCGACACCGGAGAGCGGTATCTCAGTACCGTTCTTTTCCAGGATCAGTAG

Upstream 100 bases:

>100_bases
GACACCATGGTGCACAGGACGAAGCGGCGGAGCGGCAGACCGGCAGCGGTGGTCACTCCCACTGACAACCACAATGACTC
AACAAAAGGAGCAGGGGCAC

Downstream 100 bases:

>100_bases
GAGTACCCCCGCGCCCCTGCCGGGGCCTTGGGCGGGGGTGCGGGGAGGGGTAACTAATGAACCGTCTTTTCATTGTTGTA
GTGATGCTCCTGGCTCCGCT

Product: cysteine synthase

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MAQIYENLTDLIGKTPLLRLRKVAAGIEADIVAKLESFNPGGSVKDRIGVSMIRDAEGRGLINKDTVIIEPTSGNTGIAL
AFVCAARGYRLVLTMPDTMSVERRNLLKAYGAELVLTRGAEGMKGAINKAEELAADIPNSFIPQQFRNPANPHIHRFTTA
EEIWTDTDGKVDVFVAGVGTGGTVTGVGEVLKKRNPGVKIIAVEPFDSPVLSGGHPAPHKIQGIGAGFVPEVLNREAIDE
IFTVRNEEALETARRLAREEGLLVGISAGAAAFAALEVARRPENKGKRIVVVLPDTGERYLSTVLFQDQ

Sequences:

>Translated_309_residues
MAQIYENLTDLIGKTPLLRLRKVAAGIEADIVAKLESFNPGGSVKDRIGVSMIRDAEGRGLINKDTVIIEPTSGNTGIAL
AFVCAARGYRLVLTMPDTMSVERRNLLKAYGAELVLTRGAEGMKGAINKAEELAADIPNSFIPQQFRNPANPHIHRFTTA
EEIWTDTDGKVDVFVAGVGTGGTVTGVGEVLKKRNPGVKIIAVEPFDSPVLSGGHPAPHKIQGIGAGFVPEVLNREAIDE
IFTVRNEEALETARRLAREEGLLVGISAGAAAFAALEVARRPENKGKRIVVVLPDTGERYLSTVLFQDQ
>Mature_308_residues
AQIYENLTDLIGKTPLLRLRKVAAGIEADIVAKLESFNPGGSVKDRIGVSMIRDAEGRGLINKDTVIIEPTSGNTGIALA
FVCAARGYRLVLTMPDTMSVERRNLLKAYGAELVLTRGAEGMKGAINKAEELAADIPNSFIPQQFRNPANPHIHRFTTAE
EIWTDTDGKVDVFVAGVGTGGTVTGVGEVLKKRNPGVKIIAVEPFDSPVLSGGHPAPHKIQGIGAGFVPEVLNREAIDEI
FTVRNEEALETARRLAREEGLLVGISAGAAAFAALEVARRPENKGKRIVVVLPDTGERYLSTVLFQDQ

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=313, Percent_Identity=42.8115015974441, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI295821200, Length=313, Percent_Identity=42.8115015974441, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4557415, Length=313, Percent_Identity=42.8115015974441, Blast_Score=243, Evalue=2e-64,
Organism=Escherichia coli, GI1788754, Length=317, Percent_Identity=57.0977917981073, Blast_Score=318, Evalue=2e-88,
Organism=Escherichia coli, GI2367138, Length=302, Percent_Identity=40.7284768211921, Blast_Score=224, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=53.3333333333333, Blast_Score=320, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=52, Blast_Score=306, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI115535073, Length=307, Percent_Identity=54.7231270358306, Blast_Score=301, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=52.5597269624573, Blast_Score=300, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=49.4983277591973, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=53.2710280373832, Blast_Score=228, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17534315, Length=322, Percent_Identity=39.1304347826087, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25147552, Length=320, Percent_Identity=36.875, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=47.008547008547, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321594, Length=316, Percent_Identity=39.873417721519, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6321449, Length=327, Percent_Identity=33.6391437308869, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24643623, Length=310, Percent_Identity=41.2903225806452, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI20129101, Length=310, Percent_Identity=41.2903225806452, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33142; Mature: 33011

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQIYENLTDLIGKTPLLRLRKVAAGIEADIVAKLESFNPGGSVKDRIGVSMIRDAEGRG
CCHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHCHHHEECCCCCC
LINKDTVIIEPTSGNTGIALAFVCAARGYRLVLTMPDTMSVERRNLLKAYGAELVLTRGA
CCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCEEEEECCC
EGMKGAINKAEELAADIPNSFIPQQFRNPANPHIHRFTTAEEIWTDTDGKVDVFVAGVGT
CCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEECHHHHHCCCCCCEEEEEEECCC
GGTVTGVGEVLKKRNPGVKIIAVEPFDSPVLSGGHPAPHKIQGIGAGFVPEVLNREAIDE
CCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHCHHHHHH
IFTVRNEEALETARRLAREEGLLVGISAGAAAFAALEVARRPENKGKRIVVVLPDTGERY
HHHCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHH
LSTVLFQDQ
HHHHHCCCC
>Mature Secondary Structure 
AQIYENLTDLIGKTPLLRLRKVAAGIEADIVAKLESFNPGGSVKDRIGVSMIRDAEGRG
CHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHCHHHEECCCCCC
LINKDTVIIEPTSGNTGIALAFVCAARGYRLVLTMPDTMSVERRNLLKAYGAELVLTRGA
CCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCEEEEECCC
EGMKGAINKAEELAADIPNSFIPQQFRNPANPHIHRFTTAEEIWTDTDGKVDVFVAGVGT
CCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEECHHHHHCCCCCCEEEEEEECCC
GGTVTGVGEVLKKRNPGVKIIAVEPFDSPVLSGGHPAPHKIQGIGAGFVPEVLNREAIDE
CCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCHHHHHHCHHHHHH
IFTVRNEEALETARRLAREEGLLVGISAGAAAFAALEVARRPENKGKRIVVVLPDTGERY
HHHCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHH
LSTVLFQDQ
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]