Click here to switch to the map view.

The map label for this gene is xylR [H]

Identifier: 78222754

GI number: 78222754

Start: 1746931

End: 1748691

Strand: Direct

Name: xylR [H]

Synonym: Gmet_1542

Alternate gene names: 78222754

Gene position: 1746931-1748691 (Clockwise)

Preceding gene: 78222744

Following gene: 78222770

Centisome position: 43.7

GC content: 51.16

Gene sequence:

>1761_bases
GTGAAAAAGCGTAAGCTCGCGAAGGAAAGCTCTGCAAGTACCGTGGTCAGTGACAATCTACGTTCGCAATTGCGTTTTTC
AAGCGAAACCGGCGAGATATGGTTGTCTGAGCACCGTATGCTTCTTGTTCATGCGGATTCTCATGCGAAATTGCGCCGTG
AGTTGATCGAAACTCTCGGAGTGGGCCGTGCGCGGGGCGTGATCATGCGCATAGGCTATGCTACCGGCATAAGTGATTAT
GAACTCACCCATATCAGCCGGGATAGGGTAACTGATAATGAGAATTTCATGGCCGGGCCACTCTTATTCACATTGGAAGG
AAATGCCAAGACCACTATCCCCAAATTAGAAATTGATCGGAAAGCAGGTACGTTTTATGTCGAGGCTCTCTTGGAGAATT
CCTGGGAGAGTCAGGCATACCTGCGCCATTATGGTGAGTCCACCGAAGGGGTGTGTTGGTATTTACAGGGCTACTCGAGC
GGCTATTGCTCAGCTTTTATGGGCAAGCAAATTCTTTGCAGGGAAGTAAACTGTTTCGCTGCTGGCGCGAACCACTGCTA
CCTTGTCGGTAAACCAGTCGAGGAATGGGATGATTCCAGCGATTTTTCACTAATGATGAATCAGGAATCCATTACCGACC
AGCTCATCGGTCTTCAGAATCAGGTTGTGCACTTACGTTCCGCAATGGGTGAAAAAAAATTGCCTTCTGATGTGCTGGGG
AATTCGCCTGCTTTTCTCGCCGCTTACGATCTTCTTACGCAAGCAGTTGATAGCCAGATTACGGTACTGTTGCTTGGAGA
AACAGGAGTTGGCAAGGAGGTATTTGCGCGCACTCTCCATGAGAAGGGGGCACGTAGCAAGGAATCGTTCATCGCCATCA
ATTGTGCCGCCATTCCACACGACCTTGTGGAATCCGAACTGTTTGGCGTGGAAAAAGGCGCATTTACGGGCGCACATATA
TCTCGCCCCGGGCGCTTTGAGCGAGCCGACGGAGGCACGTTATTTCTTGACGAGGTGGGAGATCTCCCCCTTTCGGCGCA
GGCAAAACTACTTCGGGTGCTTCAGGAGGGAGAGGTTGAACGTCTCGGCGGGACAAAAACACATAAAGTAAACGTGCGTC
TTGTAGCGGCAACAAACATCGACCTGCGAAACCTGGTTGAGGTGGGAAAATTCAGATCCGATCTTTATTACCGGCTCAAC
GCGCTGCAGATTAACATTCCGTCACTCAGAGAGCGTAAGTTGGACATTTTGCTTCTCGCCAAGCACTTCCTCAAGAAGTA
CTCCGCGATCAACGGCAAGAGGCTGCGCGGATTTTCCGACAAGGCAAAGCTGGCTTTGCTCGCCTATCAATGGCCCGGCA
ACATCCGTGAACTGCAGAATGTTGTCGAACGCGCTGTAATGCTTGCGCCGAACGGCACCCACATCCATATAAACCATATG
TTTCCTTCGTACAAAAACGATGGACTCCACGAGTTTGCGCTTGATGTCAAAGGGGGCTTGAGCGCCGACCAGGAGGAAAG
TGGTGAAAGCTTCTGCGATGCCATCCTGAGCGGCAGGATAACAATGGAACAGGCCAATAACATGCTGGCAAACGCCGCCG
TGGAAAAGGCCAAAGGGAACCTTTCCGCTGCGGCGAGAATGCTCGGTCTGACCCGTGCCCAGTTGGCCTATCGACTCGAG
CACCAGCATGACAATGCAATCGTCAAATCCATACGAGTCAGAGCGAAATCCCGAGATTCGTCAAACTATCCACTGAAGTG
A

Upstream 100 bases:

>100_bases
ACTGACTGTATATAATTGCAATTTAAGTAAAAGCGACATATAAAACAATGTTAGATATTGCTGGGTCACTTCTGAGGCAT
TTATCGAGAAAGGTGGCATA

Downstream 100 bases:

>100_bases
GTAATTGTTGGTTGCCGCCTGTAGCCTAAATCACTAGGGGGCTTTCTCCTTCAGGTAGGCATCCTTGCAGTGTTCCGAGC
AAAAATAGAGCGCGCGGCCG

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: 67 kDa protein [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MKKRKLAKESSASTVVSDNLRSQLRFSSETGEIWLSEHRMLLVHADSHAKLRRELIETLGVGRARGVIMRIGYATGISDY
ELTHISRDRVTDNENFMAGPLLFTLEGNAKTTIPKLEIDRKAGTFYVEALLENSWESQAYLRHYGESTEGVCWYLQGYSS
GYCSAFMGKQILCREVNCFAAGANHCYLVGKPVEEWDDSSDFSLMMNQESITDQLIGLQNQVVHLRSAMGEKKLPSDVLG
NSPAFLAAYDLLTQAVDSQITVLLLGETGVGKEVFARTLHEKGARSKESFIAINCAAIPHDLVESELFGVEKGAFTGAHI
SRPGRFERADGGTLFLDEVGDLPLSAQAKLLRVLQEGEVERLGGTKTHKVNVRLVAATNIDLRNLVEVGKFRSDLYYRLN
ALQINIPSLRERKLDILLLAKHFLKKYSAINGKRLRGFSDKAKLALLAYQWPGNIRELQNVVERAVMLAPNGTHIHINHM
FPSYKNDGLHEFALDVKGGLSADQEESGESFCDAILSGRITMEQANNMLANAAVEKAKGNLSAAARMLGLTRAQLAYRLE
HQHDNAIVKSIRVRAKSRDSSNYPLK

Sequences:

>Translated_586_residues
MKKRKLAKESSASTVVSDNLRSQLRFSSETGEIWLSEHRMLLVHADSHAKLRRELIETLGVGRARGVIMRIGYATGISDY
ELTHISRDRVTDNENFMAGPLLFTLEGNAKTTIPKLEIDRKAGTFYVEALLENSWESQAYLRHYGESTEGVCWYLQGYSS
GYCSAFMGKQILCREVNCFAAGANHCYLVGKPVEEWDDSSDFSLMMNQESITDQLIGLQNQVVHLRSAMGEKKLPSDVLG
NSPAFLAAYDLLTQAVDSQITVLLLGETGVGKEVFARTLHEKGARSKESFIAINCAAIPHDLVESELFGVEKGAFTGAHI
SRPGRFERADGGTLFLDEVGDLPLSAQAKLLRVLQEGEVERLGGTKTHKVNVRLVAATNIDLRNLVEVGKFRSDLYYRLN
ALQINIPSLRERKLDILLLAKHFLKKYSAINGKRLRGFSDKAKLALLAYQWPGNIRELQNVVERAVMLAPNGTHIHINHM
FPSYKNDGLHEFALDVKGGLSADQEESGESFCDAILSGRITMEQANNMLANAAVEKAKGNLSAAARMLGLTRAQLAYRLE
HQHDNAIVKSIRVRAKSRDSSNYPLK
>Mature_586_residues
MKKRKLAKESSASTVVSDNLRSQLRFSSETGEIWLSEHRMLLVHADSHAKLRRELIETLGVGRARGVIMRIGYATGISDY
ELTHISRDRVTDNENFMAGPLLFTLEGNAKTTIPKLEIDRKAGTFYVEALLENSWESQAYLRHYGESTEGVCWYLQGYSS
GYCSAFMGKQILCREVNCFAAGANHCYLVGKPVEEWDDSSDFSLMMNQESITDQLIGLQNQVVHLRSAMGEKKLPSDVLG
NSPAFLAAYDLLTQAVDSQITVLLLGETGVGKEVFARTLHEKGARSKESFIAINCAAIPHDLVESELFGVEKGAFTGAHI
SRPGRFERADGGTLFLDEVGDLPLSAQAKLLRVLQEGEVERLGGTKTHKVNVRLVAATNIDLRNLVEVGKFRSDLYYRLN
ALQINIPSLRERKLDILLLAKHFLKKYSAINGKRLRGFSDKAKLALLAYQWPGNIRELQNVVERAVMLAPNGTHIHINHM
FPSYKNDGLHEFALDVKGGLSADQEESGESFCDAILSGRITMEQANNMLANAAVEKAKGNLSAAARMLGLTRAQLAYRLE
HQHDNAIVKSIRVRAKSRDSSNYPLK

Specific function: Regulatory protein of the TOL plasmid xyl operons. In the presence of m-xylene or m-methylbenzyl alcohol xylR activates both the xylCMABN operon and the regulatory gene xylS; xylS itself activates the xylXYZLTEGFJQKIH operon. XylR interacts with sigma- 54

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=347, Percent_Identity=43.2276657060519, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI87082117, Length=250, Percent_Identity=52.8, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1790437, Length=322, Percent_Identity=45.0310559006211, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1789087, Length=262, Percent_Identity=48.0916030534351, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1790299, Length=334, Percent_Identity=40.1197604790419, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI87081872, Length=328, Percent_Identity=41.1585365853659, Blast_Score=225, Evalue=7e-60,
Organism=Escherichia coli, GI1788905, Length=331, Percent_Identity=39.5770392749245, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1789233, Length=364, Percent_Identity=35.4395604395604, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI87082152, Length=234, Percent_Identity=45.7264957264957, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1786524, Length=349, Percent_Identity=38.10888252149, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1787583, Length=205, Percent_Identity=40.9756097560976, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1789828, Length=227, Percent_Identity=40.0881057268723, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081858, Length=238, Percent_Identity=34.453781512605, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR004096
- InterPro:   IPR010523
- ProDom:   PD485437 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat; PF02830 V4R; PF06505 XylR_N [H]

EC number: NA

Molecular weight: Translated: 65084; Mature: 65084

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRKLAKESSASTVVSDNLRSQLRFSSETGEIWLSEHRMLLVHADSHAKLRRELIETLG
CCCHHHCCCCCCCHHHHHHHHHHHEECCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHC
VGRARGVIMRIGYATGISDYELTHISRDRVTDNENFMAGPLLFTLEGNAKTTIPKLEIDR
CCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCCCCCCCCEEECC
KAGTFYVEALLENSWESQAYLRHYGESTEGVCWYLQGYSSGYCSAFMGKQILCREVNCFA
CCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHCCEEE
AGANHCYLVGKPVEEWDDSSDFSLMMNQESITDQLIGLQNQVVHLRSAMGEKKLPSDVLG
ECCCEEEEECCCHHHCCCCCCEEEEECCHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHC
NSPAFLAAYDLLTQAVDSQITVLLLGETGVGKEVFARTLHEKGARSKESFIAINCAAIPH
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCH
DLVESELFGVEKGAFTGAHISRPGRFERADGGTLFLDEVGDLPLSAQAKLLRVLQEGEVE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHH
RLGGTKTHKVNVRLVAATNIDLRNLVEVGKFRSDLYYRLNALQINIPSLRERKLDILLLA
HCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHHH
KHFLKKYSAINGKRLRGFSDKAKLALLAYQWPGNIRELQNVVERAVMLAPNGTHIHINHM
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHEEECCCCCEEEEEEC
FPSYKNDGLHEFALDVKGGLSADQEESGESFCDAILSGRITMEQANNMLANAAVEKAKGN
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCC
LSAAARMLGLTRAQLAYRLEHQHDNAIVKSIRVRAKSRDSSNYPLK
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKRKLAKESSASTVVSDNLRSQLRFSSETGEIWLSEHRMLLVHADSHAKLRRELIETLG
CCCHHHCCCCCCCHHHHHHHHHHHEECCCCCCEEEECCEEEEEECCCHHHHHHHHHHHHC
VGRARGVIMRIGYATGISDYELTHISRDRVTDNENFMAGPLLFTLEGNAKTTIPKLEIDR
CCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCCCCCCCCEEECC
KAGTFYVEALLENSWESQAYLRHYGESTEGVCWYLQGYSSGYCSAFMGKQILCREVNCFA
CCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHCCEEE
AGANHCYLVGKPVEEWDDSSDFSLMMNQESITDQLIGLQNQVVHLRSAMGEKKLPSDVLG
ECCCEEEEECCCHHHCCCCCCEEEEECCHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHC
NSPAFLAAYDLLTQAVDSQITVLLLGETGVGKEVFARTLHEKGARSKESFIAINCAAIPH
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCH
DLVESELFGVEKGAFTGAHISRPGRFERADGGTLFLDEVGDLPLSAQAKLLRVLQEGEVE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHH
RLGGTKTHKVNVRLVAATNIDLRNLVEVGKFRSDLYYRLNALQINIPSLRERKLDILLLA
HCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHHH
KHFLKKYSAINGKRLRGFSDKAKLALLAYQWPGNIRELQNVVERAVMLAPNGTHIHINHM
HHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHEEECCCCCEEEEEEC
FPSYKNDGLHEFALDVKGGLSADQEESGESFCDAILSGRITMEQANNMLANAAVEKAKGN
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCC
LSAAARMLGLTRAQLAYRLEHQHDNAIVKSIRVRAKSRDSSNYPLK
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3169574; 2993247; 2430049 [H]